{"id":"https://openalex.org/W4200525366","doi":"https://doi.org/10.1093/ofid/ofab466.1220","title":"1026. Following the Hoof Prints: Detecting Coxiella and Brucella infections with A Plasma-based Microbial Cell-Free DNA Next-generation Sequencing Test","display_name":"1026. Following the Hoof Prints: Detecting Coxiella and Brucella infections with A Plasma-based Microbial Cell-Free DNA Next-generation Sequencing Test","publication_year":2021,"publication_date":"2021-11-01","ids":{"openalex":"https://openalex.org/W4200525366","doi":"https://doi.org/10.1093/ofid/ofab466.1220","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8644969"},"language":"en","primary_location":{"id":"doi:10.1093/ofid/ofab466.1220","doi":"https://doi.org/10.1093/ofid/ofab466.1220","is_oa":true,"landing_page_url":"https://doi.org/10.1093/ofid/ofab466.1220","pdf_url":null,"source":{"id":"https://openalex.org/S2735126445","display_name":"Open Forum Infectious Diseases","issn_l":"2328-8957","issn":["2328-8957"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Open Forum Infectious Diseases","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W4200525366"],"child_works_count":0,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1093/ofid/ofab466.1220","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003676684","display_name":"Nicholas R. Degner","orcid":"https://orcid.org/0000-0003-4578-3726","observed_orcids":["https://orcid.org/0000-0003-4578-3726"]},"institutions":[{"id":"https://openalex.org/I4405267391","display_name":"Karius, Inc. (United States)","ror":"https://ror.org/02jemby36","country_code":"US","type":"company","lineage":["https://openalex.org/I4405267391"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nicholas R Degner","raw_affiliation_strings":["Karius Inc., San Francisco, California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karius Inc., San Francisco, California","institution_ids":["https://openalex.org/I4405267391"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017283910","display_name":"Ricardo Castillo-Galvan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405267391","display_name":"Karius, Inc. (United States)","ror":"https://ror.org/02jemby36","country_code":"US","type":"company","lineage":["https://openalex.org/I4405267391"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ricardo Castillo-Galvan","raw_affiliation_strings":["Karius, Inc., Franklin, Tennessee"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karius, Inc., Franklin, Tennessee","institution_ids":["https://openalex.org/I4405267391"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044938300","display_name":"Jose Alexander","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405267391","display_name":"Karius, Inc. (United States)","ror":"https://ror.org/02jemby36","country_code":"US","type":"company","lineage":["https://openalex.org/I4405267391"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jose Alexander","raw_affiliation_strings":["Karius, Inc., Franklin, Tennessee"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karius, Inc., Franklin, Tennessee","institution_ids":["https://openalex.org/I4405267391"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057669149","display_name":"Aparna Arun","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405267391","display_name":"Karius, Inc. (United States)","ror":"https://ror.org/02jemby36","country_code":"US","type":"company","lineage":["https://openalex.org/I4405267391"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aparna Arun","raw_affiliation_strings":["Karius, Inc., Franklin, Tennessee"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karius, Inc., Franklin, Tennessee","institution_ids":["https://openalex.org/I4405267391"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011693820","display_name":"Christiaan R. de Vries","orcid":"https://orcid.org/0000-0002-4667-5973","observed_orcids":["https://orcid.org/0000-0002-4667-5973"]},"institutions":[{"id":"https://openalex.org/I4405267391","display_name":"Karius, Inc. (United States)","ror":"https://ror.org/02jemby36","country_code":"US","type":"company","lineage":["https://openalex.org/I4405267391"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christiaan R de Vries","raw_affiliation_strings":["Karius, Redwood City, California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karius, Redwood City, California","institution_ids":["https://openalex.org/I4405267391"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035980169","display_name":"Ann T. MacIntyre","orcid":"https://orcid.org/0000-0003-4410-113X","observed_orcids":["https://orcid.org/0000-0003-4410-113X"]},"institutions":[{"id":"https://openalex.org/I4405267391","display_name":"Karius, Inc. (United States)","ror":"https://ror.org/02jemby36","country_code":"US","type":"company","lineage":["https://openalex.org/I4405267391"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ann Macintyre","raw_affiliation_strings":["Karius, Redwood City, California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karius, Redwood City, California","institution_ids":["https://openalex.org/I4405267391"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051228915","display_name":"Bradley A. Perkins","orcid":"https://orcid.org/0009-0003-4542-2805","observed_orcids":["https://orcid.org/0009-0003-4542-2805"]},"institutions":[{"id":"https://openalex.org/I4405267391","display_name":"Karius, Inc. (United States)","ror":"https://ror.org/02jemby36","country_code":"US","type":"company","lineage":["https://openalex.org/I4405267391"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bradley Perkins","raw_affiliation_strings":["Karius, Inc., Franklin, Tennessee"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karius, Inc., Franklin, Tennessee","institution_ids":["https://openalex.org/I4405267391"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013495583","display_name":"Asim A. Ahmed","orcid":"https://orcid.org/0000-0003-1340-8825","observed_orcids":["https://orcid.org/0000-0003-1340-8825"]},"institutions":[{"id":"https://openalex.org/I4405267391","display_name":"Karius, Inc. (United States)","ror":"https://ror.org/02jemby36","country_code":"US","type":"company","lineage":["https://openalex.org/I4405267391"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Asim A Ahmed","raw_affiliation_strings":["Karius, Inc, Redwood City, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karius, Inc, Redwood City, CA","institution_ids":["https://openalex.org/I4405267391"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088941685","display_name":"Matthew Smollin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405267391","display_name":"Karius, Inc. (United States)","ror":"https://ror.org/02jemby36","country_code":"US","type":"company","lineage":["https://openalex.org/I4405267391"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Matthew Smollin","raw_affiliation_strings":["Karius, Inc., Franklin, Tennessee"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karius, Inc., Franklin, Tennessee","institution_ids":["https://openalex.org/I4405267391"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4405267391"],"apc_list":{"value":3465,"currency":"USD","value_usd":3465},"apc_paid":{"value":3465,"currency":"USD","value_usd":3465},"fwci":null,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"8","issue":"Supplement_1","first_page":"S604","last_page":"S604"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11899","display_name":"Brucella: diagnosis, epidemiology, treatment","score":0.7666000127792358,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11899","display_name":"Brucella: diagnosis, epidemiology, treatment","score":0.7666000127792358,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.16410000622272491,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":0.0340999998152256,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/coxiella-burnetii","display_name":"Coxiella burnetii","score":0.9760000109672546},{"id":"https://openalex.org/keywords/brucella-melitensis","display_name":"Brucella melitensis","score":0.9819999933242798},{"id":"https://openalex.org/keywords/q-fever","display_name":"Q fever","score":0.9660000205039978},{"id":"https://openalex.org/keywords/brucellosis","display_name":"brucellosis","score":0.9959999918937683},{"id":"https://openalex.org/keywords/microbial-cell-free-dna","display_name":"microbial cell-free DNA","score":0.9539999961853027},{"id":"https://openalex.org/keywords/karius-test","display_name":"Karius test","score":0.8519999980926514},{"id":"https://openalex.org/keywords/fever-of-unknown-origin","display_name":"fever of unknown origin","score":0.7089999914169312},{"id":"https://openalex.org/keywords/endocarditis","display_name":"endocarditis","score":0.6819999814033508},{"id":"https://openalex.org/keywords/vertebral-osteomyelitis","display_name":"vertebral osteomyelitis","score":0.8389999866485596},{"id":"https://openalex.org/keywords/epidural-abscess","display_name":"epidural abscess","score":0.9409999847412109},{"id":"https://openalex.org/keywords/non-invasive-diagnosis","display_name":"non-invasive diagnosis","score":0.640999972820282},{"id":"https://openalex.org/keywords/culture-negative-infection","display_name":"culture-negative infection","score":0.4880000054836273}],"concepts":[{"id":"https://openalex.org/C2776607032","wikidata":"https://www.wikidata.org/wiki/Q133971","display_name":"Coxiella burnetii","level":2,"score":0.9563238620758057},{"id":"https://openalex.org/C2780767784","wikidata":"https://www.wikidata.org/wiki/Q313358","display_name":"Brucella","level":3,"score":0.7628233432769775},{"id":"https://openalex.org/C2781429187","wikidata":"https://www.wikidata.org/wiki/Q156050","display_name":"Brucellosis","level":2,"score":0.6978665590286255},{"id":"https://openalex.org/C2776353676","wikidata":"https://www.wikidata.org/wiki/Q182672","display_name":"Zoonosis","level":2,"score":0.5962216854095459},{"id":"https://openalex.org/C2780731870","wikidata":"https://www.wikidata.org/wiki/Q164818","display_name":"Q fever","level":2,"score":0.5296570658683777},{"id":"https://openalex.org/C2777692091","wikidata":"https://www.wikidata.org/wiki/Q3512031","display_name":"Brucella melitensis","level":4,"score":0.4839901924133301},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.47708162665367126},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.40995118021965027},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.36190545558929443},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3540838062763214}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1093/ofid/ofab466.1220","doi":"https://doi.org/10.1093/ofid/ofab466.1220","is_oa":true,"landing_page_url":"https://doi.org/10.1093/ofid/ofab466.1220","pdf_url":null,"source":{"id":"https://openalex.org/S2735126445","display_name":"Open Forum Infectious Diseases","issn_l":"2328-8957","issn":["2328-8957"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Open Forum Infectious Diseases","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8644969","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8644969/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8644969/pdf/","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Open Forum Infect Dis","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/ofid/ofab466.1220","doi":"https://doi.org/10.1093/ofid/ofab466.1220","is_oa":true,"landing_page_url":"https://doi.org/10.1093/ofid/ofab466.1220","pdf_url":null,"source":{"id":"https://openalex.org/S2735126445","display_name":"Open Forum Infectious Diseases","issn_l":"2328-8957","issn":["2328-8957"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Open Forum Infectious Diseases","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9763000011444092}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4200525366.pdf","grobid_xml":"https://content.openalex.org/works/W4200525366.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4200047175","https://openalex.org/W7220744529","https://openalex.org/W4200491317","https://openalex.org/W7108079765","https://openalex.org/W4414262336","https://openalex.org/W3202989356","https://openalex.org/W7171369495","https://openalex.org/W4414608717","https://openalex.org/W4282833007","https://openalex.org/W4384120642"],"abstract_inverted_index":{"Abstract":[0],"Background":[1],"Coxiella":[2,172,200,234],"burnetii":[3,201],"and":[4,15,24,44,69,80,114,134,141,164,173,206,254,267,278,313],"Brucella":[5,174,210,261],"spp.":[6],"are":[7,139,144,162],"zoonotic":[8,307],"bacterial":[9],"pathogens":[10,19,335],"responsible":[11],"for":[12,88],"Q":[13,49,311],"fever":[14,50,245,312],"Brucellosis,":[16],"respectively.":[17],"Both":[18],"have":[20],"a":[21,101,147,159,297],"global":[22],"distribution":[23],"Brucellosis":[25,314],"is":[26,132],"the":[27,32,35,67,74,82,226,233,324,327],"most":[28],"common":[29],"zoonosis":[30],"in":[31,116,122,128,166,225,237,264],"world.":[33],"However,":[34],"CDC":[36],"reports":[37],"only":[38],"80-120":[39],"cases":[40,46,198,208,243,250,281,322],"of":[41,47,54,71,84,150,171,199,209,222,232,244,246,251,257,282,306,326],"human":[42,137],"brucellosis":[43],"~150":[45],"acute":[48],"annually.":[51],"The":[52,109],"diagnosis":[53],"these":[55,89,106,321],"infections":[56,308,331],"can":[57],"be":[58],"limited":[59],"by:":[60],"(1)":[61],"their":[62],"difficulty":[63],"to":[64,104,146,275,301,329],"culture;":[65],"(2)":[66],"insensitivity":[68],"nonspecificity":[70],"serology;":[72],"(3)":[73],"clinical":[75,182,304,338],"overlap":[76],"with":[77,186,241,273,336],"other":[78],"infections;":[79],"(4)":[81],"unreliability":[83],"epidemiological":[85],"exposure":[86,274],"history":[87],"zoonoses.":[90],"Unbiased":[91],"microbial":[92],"cell":[93],"free":[94],"DNA":[95],"(mcfDNA)":[96],"next-generation":[97],"sequencing":[98],"(NGS)":[99],"offers":[100],"potential":[102,325],"solution":[103],"overcome":[105],"limitations.":[107],"Methods":[108],"Karius":[110,347,355,367,372],"TestTM":[111],"(KT)":[112],"developed":[113],"validated":[115],"Karius\u2019s":[117],"CLIA":[118],"certified/CAP":[119],"accredited":[120],"lab":[121],"Redwood":[123],"City,":[124],"CA":[125],"detects":[126],"mcfDNA":[127,131,294],"plasma.":[129],"After":[130],"extracted":[133],"NGS":[135,295],"performed,":[136],"reads":[138],"removed,":[140],"remaining":[142],"sequences":[143],"aligned":[145],"curated":[148],"database":[149],">":[151],"1500":[152],"organisms.":[153],"McfDNA":[154],"from":[155,177],"organisms":[156],"present":[157],"above":[158],"statistical":[160],"threshold":[161],"reported":[163],"quantified":[165],"molecules/\u00b5L":[167],"(MPM).":[168],"KT":[169,195,328],"detections":[170,224,235,262],"were":[175,236],"reviewed":[176],"August":[178],"2017":[179],"-":[180],"present;":[181],"information":[183],"was":[184],"obtained":[185],"test":[187,300],"requisition":[188],"or":[189],"consultation":[190],"upon":[191],"result":[192],"reporting.":[193],"Results":[194],"detected":[196],"8":[197],"(1735":[202],"MPM":[203,214],"+/-":[204,215],"3000)":[205],"5":[207,242],"melitensis":[211],"(avg":[212],"296":[213],"223)":[216],"(Table":[217],"1),":[218],"representing":[219],"approximately":[220],"1-2%":[221],"all":[223],"US":[227],"during":[228],"this":[229],"period.":[230],"All":[231],"adults":[238,266],"(100%":[239],"male)":[240],"unknown":[247],"origin,":[248],"2":[249,268],"culture-negative":[252],"endocarditis":[253],"one":[255],"case":[256],"endovascular":[258],"graft":[259],"infection.":[260],"occurred":[263],"3":[265,272,280],"children":[269],"(60%":[270],"male),":[271],"unpasteurized":[276],"dairy":[277],"included":[279],"spine":[283],"infection":[284],"(2":[285],"vertebral":[286],"osteomyelitis,":[287],"1":[288],"epidural":[289],"abscess).":[290],"Conclusion":[291],"Open-ended,":[292],"plasma-based":[293],"provides":[296],"rapid,":[298],"non-invasive":[299],"diagnose":[302,330],"diverse":[303],"manifestations":[305],"such":[309],"as":[310],"against":[315],"competing":[316],"broad":[317],"differential":[318],"diagnoses.":[319],"Furthermore,":[320],"highlight":[323],"caused":[332],"by":[333],"fastidious/unculturable":[334],"cryptic":[337],"presentations.":[339],"Disclosures":[340],"Nicholas":[341],"R.":[342],"Degner,":[343],"MD,":[344,360,371,382,389],"MPH,":[345,354],"MS,":[346],"Inc.":[348,356,378,384,391,397],"(Employee,":[349],"Shareholder)":[350],"Ricardo":[351],"Castillo-Galvan,":[352],"MD":[353],"(Consultant)":[357],"Jose":[358],"Alexander,":[359],"D(ABMM),":[361],"FCCM,":[362],"CIC,":[363],"SM,":[364],"MB(ASCP),":[365],"BCMAS,":[366],"(Employee)":[368,373,379,385,392,398],"Aparna":[369],"Arun,":[370],"Ann":[374],"Macintyre,":[375],"DO,":[376],"Karius,":[377,383,390,396],"Bradley":[380],"Perkins,":[381],"Asim":[386],"A.":[387],"Ahmed,":[388],"Matthew":[393],"Smollin,":[394],"PharmD,":[395]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2021-12-31T00:00:00"}