{"id":"https://openalex.org/W4200324876","doi":"https://doi.org/10.21203/rs.3.rs-1100361/v1","title":"Monosialoganglioside GM1 Deficiency Inhibits the Neurotrophic Effects of GDNF by Disrupting Lipid Raft Assembly","display_name":"Monosialoganglioside GM1 Deficiency Inhibits the Neurotrophic Effects of GDNF by Disrupting Lipid Raft Assembly","publication_year":2021,"publication_date":"2021-12-02","ids":{"openalex":"https://openalex.org/W4200324876","doi":"https://doi.org/10.21203/rs.3.rs-1100361/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-1100361/v1","doi":"https://doi.org/10.21203/rs.3.rs-1100361/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-1100361/v1","pdf_url":"https://www.researchsquare.com/article/rs-1100361/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.researchsquare.com/article/rs-1100361/latest.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040722674","display_name":"Shimin Jiang","orcid":"https://orcid.org/0000-0002-1795-8587","observed_orcids":["https://orcid.org/0000-0002-1795-8587"]},"institutions":[{"id":"https://openalex.org/I177388780","display_name":"Xuzhou Medical College","ror":"https://ror.org/04fe7hy80","country_code":"CN","type":"education","lineage":["https://openalex.org/I177388780"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shimin Jiang","raw_affiliation_strings":["Xuzhou Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Xuzhou Medical University","institution_ids":["https://openalex.org/I177388780"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102046313","display_name":"Tai Zhou","orcid":"https://orcid.org/0009-0004-8096-8229","observed_orcids":["https://orcid.org/0009-0004-8096-8229"]},"institutions":[{"id":"https://openalex.org/I177388780","display_name":"Xuzhou Medical College","ror":"https://ror.org/04fe7hy80","country_code":"CN","type":"education","lineage":["https://openalex.org/I177388780"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Tai Zhou","raw_affiliation_strings":["Xuzhou Medical University","Xuzhou Medical University Zhongcheng Wang Xuzhou Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Xuzhou Medical University","institution_ids":["https://openalex.org/I177388780"]},{"raw_affiliation_string":"Xuzhou Medical University Zhongcheng Wang Xuzhou Medical University","institution_ids":["https://openalex.org/I177388780"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100614385","display_name":"Kejia Zhang","orcid":"https://orcid.org/0000-0002-6970-8571","observed_orcids":["https://orcid.org/0000-0002-6970-8571"]},"institutions":[{"id":"https://openalex.org/I177388780","display_name":"Xuzhou Medical College","ror":"https://ror.org/04fe7hy80","country_code":"CN","type":"education","lineage":["https://openalex.org/I177388780"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Kejia Zhang","raw_affiliation_strings":["Xuzhou Medical University","Xuzhou Medical University Zhongcheng Wang Xuzhou Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Xuzhou Medical University","institution_ids":["https://openalex.org/I177388780"]},{"raw_affiliation_string":"Xuzhou Medical University Zhongcheng Wang Xuzhou Medical University","institution_ids":["https://openalex.org/I177388780"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045504014","display_name":"Yao Zhou","orcid":"https://orcid.org/0000-0002-2845-6339","observed_orcids":["https://orcid.org/0000-0002-2845-6339"]},"institutions":[{"id":"https://openalex.org/I177388780","display_name":"Xuzhou Medical College","ror":"https://ror.org/04fe7hy80","country_code":"CN","type":"education","lineage":["https://openalex.org/I177388780"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yao Zhou","raw_affiliation_strings":["Xuzhou Medical University","Xuzhou Medical University Zhongcheng Wang Xuzhou Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Xuzhou Medical University","institution_ids":["https://openalex.org/I177388780"]},{"raw_affiliation_string":"Xuzhou Medical University Zhongcheng Wang Xuzhou Medical University","institution_ids":["https://openalex.org/I177388780"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101501945","display_name":"Zhongcheng Wang","orcid":"https://orcid.org/0000-0001-8971-7742","observed_orcids":["https://orcid.org/0000-0001-8971-7742"]},"institutions":[{"id":"https://openalex.org/I177388780","display_name":"Xuzhou Medical College","ror":"https://ror.org/04fe7hy80","country_code":"CN","type":"education","lineage":["https://openalex.org/I177388780"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zhongcheng Wang","raw_affiliation_strings":["Xuzhou Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Xuzhou Medical University","institution_ids":["https://openalex.org/I177388780"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100361065","display_name":"Li Li","orcid":"https://orcid.org/0000-0001-9461-596X","observed_orcids":["https://orcid.org/0000-0001-9461-596X"]},"institutions":[{"id":"https://openalex.org/I177388780","display_name":"Xuzhou Medical College","ror":"https://ror.org/04fe7hy80","country_code":"CN","type":"education","lineage":["https://openalex.org/I177388780"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Li Li","raw_affiliation_strings":["Xuzhou Medical University"],"raw_orcid":"https://orcid.org/0000-0001-9461-596X","affiliations":[{"raw_affiliation_string":"Xuzhou Medical University","institution_ids":["https://openalex.org/I177388780"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5100361065"],"corresponding_institution_ids":["https://openalex.org/I177388780"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10085","display_name":"Parkinson's Disease Mechanisms and Treatments","score":0.21439999341964722,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10085","display_name":"Parkinson's Disease Mechanisms and Treatments","score":0.21439999341964722,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10483","display_name":"Nerve injury and regeneration","score":0.18379999697208405,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10945","display_name":"Lysosomal Storage Disorders Research","score":0.18150000274181366,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics (clinical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glial-cell-line-derived-neurotrophic-factor","display_name":"glial cell line-derived neurotrophic factor","score":0.972000002861023},{"id":"https://openalex.org/keywords/ret-signaling","display_name":"RET signaling","score":0.6259999871253967},{"id":"https://openalex.org/keywords/lipid-rafts","display_name":"lipid rafts","score":0.9909999966621399},{"id":"https://openalex.org/keywords/parkinson-s-disease","display_name":"Parkinson's disease","score":0.8579999804496765},{"id":"https://openalex.org/keywords/prescription-drug-monitoring-programs","display_name":"prescription drug monitoring programs","score":0.718999981880188},{"id":"https://openalex.org/keywords/sh-sy5y-cells","display_name":"SH-SY5Y cells","score":0.8960000276565552},{"id":"https://openalex.org/keywords/caveolin-1","display_name":"caveolin-1","score":0.7559999823570251},{"id":"https://openalex.org/keywords/flotillin-1","display_name":"flotillin-1","score":1.0},{"id":"https://openalex.org/keywords/neurotrophic-effects","display_name":"neurotrophic effects","score":0.6639999747276306}],"concepts":[{"id":"https://openalex.org/C185856081","wikidata":"https://www.wikidata.org/wiki/Q14916286","display_name":"Glial cell line-derived neurotrophic factor","level":4,"score":0.9032509326934814},{"id":"https://openalex.org/C9132879","wikidata":"https://www.wikidata.org/wiki/Q17100313","display_name":"Raft","level":4,"score":0.6813438534736633},{"id":"https://openalex.org/C79266657","wikidata":"https://www.wikidata.org/wiki/Q424178","display_name":"Lipid raft","level":3,"score":0.6203570365905762},{"id":"https://openalex.org/C92020748","wikidata":"https://www.wikidata.org/wiki/Q420457","display_name":"Neurotrophin","level":3,"score":0.6050549745559692},{"id":"https://openalex.org/C160539049","wikidata":"https://www.wikidata.org/wiki/Q3064187","display_name":"Neurotrophic factors","level":3,"score":0.5522829294204712},{"id":"https://openalex.org/C94296188","wikidata":"https://www.wikidata.org/wiki/Q5513091","display_name":"GDNF family of ligands","level":5,"score":0.49999117851257324},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4777202308177948},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.43153998255729675},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4293994605541229},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.19944196939468384},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1918419599533081},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.0752485990524292},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.06581079959869385},{"id":"https://openalex.org/C15920480","wikidata":"https://www.wikidata.org/wiki/Q421281","display_name":"Copolymer","level":3,"score":0.0},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-1100361/v1","doi":"https://doi.org/10.21203/rs.3.rs-1100361/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-1100361/v1","pdf_url":"https://www.researchsquare.com/article/rs-1100361/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-1100361/v1","doi":"https://doi.org/10.21203/rs.3.rs-1100361/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-1100361/v1","pdf_url":"https://www.researchsquare.com/article/rs-1100361/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2308592945","display_name":null,"funder_award_id":"81401056","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4200324876.pdf","grobid_xml":"https://content.openalex.org/works/W4200324876.grobid-xml"},"referenced_works_count":29,"referenced_works":["https://openalex.org/W1650022805","https://openalex.org/W1971208141","https://openalex.org/W1997600119","https://openalex.org/W2012246166","https://openalex.org/W2013139563","https://openalex.org/W2016730284","https://openalex.org/W2027600274","https://openalex.org/W2054810445","https://openalex.org/W2064354608","https://openalex.org/W2087131780","https://openalex.org/W2087713361","https://openalex.org/W2108598406","https://openalex.org/W2142563601","https://openalex.org/W2149917767","https://openalex.org/W2179386188","https://openalex.org/W2303974212","https://openalex.org/W2312705086","https://openalex.org/W2333911275","https://openalex.org/W2411113549","https://openalex.org/W2412904829","https://openalex.org/W2598168513","https://openalex.org/W2753184583","https://openalex.org/W2808399879","https://openalex.org/W2907925544","https://openalex.org/W2973720548","https://openalex.org/W3003365674","https://openalex.org/W3022072199","https://openalex.org/W3092264819","https://openalex.org/W3152616949"],"related_works":["https://openalex.org/W3022072199","https://openalex.org/W2753184583","https://openalex.org/W2059572565","https://openalex.org/W1461170193","https://openalex.org/W1991365348","https://openalex.org/W2064821389","https://openalex.org/W2465985806","https://openalex.org/W4206297637","https://openalex.org/W2303974212","https://openalex.org/W1968640414"],"abstract_inverted_index":{"Abstract":[0],"Recent":[1],"studies":[2],"have":[3],"shown":[4],"that":[5,125,198],"monosialoganglioside":[6],"GM1":[7,48,72,200],"deficiency":[8,73,201],"can":[9],"inhibit":[10],"the":[11,28,45,69,75,87,111,129,141,161,204,214],"signal":[12],"transduction":[13],"process":[14],"of":[15,30,47,71,79,106,113,133,143,164,210,216],"glial":[16],"cell":[17,58],"line-derived":[18],"neurotrophic":[19,205],"factor":[20],"(GDNF),":[21],"which":[22],"plays":[23],"an":[24],"important":[25],"role":[26],"in":[27],"pathogenesis":[29],"Parkinson's":[31],"disease":[32],"(PD).":[33],"However,":[34],"its":[35,146],"specific":[36],"mechanism":[37],"still":[38],"needs":[39],"to":[40,67,171],"be":[41,188],"explored.":[42],"We":[43,123],"inhibited":[44,128],"expression":[46],"by":[49,83,95,137,191,212],"treating":[50],"cells":[51,81,135],"with":[52],"D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol":[53],"(PDMP).":[54],"CCK-8":[55],"assay,":[56],"EdU":[57],"proliferation":[59,76,130],"assay":[60,64],"and":[61,77,85,99,110,131,139,145,151,159,175,207],"Western":[62],"blot":[63],"were":[65,93,118],"used":[66],"evaluate":[68],"effect":[70],"on":[74,86],"differentiation":[78,132],"SH-SY5Y":[80,134],"induced":[82,136],"GDNF":[84,138,211],"GDNF-RET":[88],"signaling":[89,148],"pathway.":[90],"Lipid":[91],"rafts":[92,117,170,181],"isolated":[94],"Triton":[96],"X-100":[97],"solubilization":[98],"OptiPrepTM":[100],"density":[101],"gradient":[102],"centrifugation.":[103],"The":[104],"alterations":[105,186],"lipid":[107,116,165,169,180,217],"raft":[108,173,218],"assembly":[109,215],"translocation":[112,178],"RET":[114,144,177],"into":[115,179],"evaluated":[119],"after":[120,155],"PDMP":[121,126,156],"treatment.":[122],"found":[124],"treatment":[127],"reduced":[140],"phosphorylation":[142],"downstream":[147,209],"molecules":[149],"Erk":[150],"Akt.":[152],"In":[153],"addition,":[154],"treatment,":[157],"caveolin-1":[158],"flotillin-1,":[160],"prototypical":[162],"markers":[163],"rafts,":[166],"diffused":[167],"from":[168],"non-lipid":[172],"microdomains,":[174],"GDNF-induced":[176],"was":[182],"also":[183],"reduced.":[184],"These":[185],"could":[187,202],"partially":[189],"reversed":[190],"adding":[192],"exogenous":[193],"GM1.":[194],"Our":[195],"results":[196],"suggest":[197],"ganglioside":[199],"compromise":[203],"effects":[206],"signals":[208],"altering":[213],"membrane":[219],"microdomains.":[220]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2021-12-31T00:00:00"}