{"id":"https://openalex.org/W4200317249","doi":"https://doi.org/10.1186/s13007-021-00832-4","title":"Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin","display_name":"Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin","publication_year":2021,"publication_date":"2021-12-01","ids":{"openalex":"https://openalex.org/W4200317249","doi":"https://doi.org/10.1186/s13007-021-00832-4","pmid":"https://pubmed.ncbi.nlm.nih.gov/34963500","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8714437"},"language":"en","primary_location":{"id":"doi:10.1186/s13007-021-00832-4","doi":"https://doi.org/10.1186/s13007-021-00832-4","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13007-021-00832-4","pdf_url":"https://link.springer.com/content/pdf/10.1186/s13007-021-00832-4.pdf","source":{"id":"https://openalex.org/S184470774","display_name":"Plant Methods","issn_l":"1746-4811","issn":["1746-4811"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Methods","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://link.springer.com/content/pdf/10.1186/s13007-021-00832-4.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059485387","display_name":"Davide Mancinotti","orcid":"https://orcid.org/0000-0002-4075-9893","observed_orcids":["https://orcid.org/0000-0002-4075-9893"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Davide Mancinotti","raw_affiliation_strings":["Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100733198","display_name":"Mar\u00eda Cecilia Rodriguez","orcid":"https://orcid.org/0000-0003-0024-5174","observed_orcids":["https://orcid.org/0000-0003-0024-5174"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Maria Cecilia Rodriguez","raw_affiliation_strings":["Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067135719","display_name":"Karen Michiko Frick","orcid":"https://orcid.org/0000-0002-5635-6693","observed_orcids":["https://orcid.org/0000-0002-5635-6693"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Karen Michiko Frick","raw_affiliation_strings":["Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036802639","display_name":"Bj\u00f8rn Dueholm","orcid":"https://orcid.org/0000-0001-5443-6959","observed_orcids":["https://orcid.org/0000-0001-5443-6959"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Bj\u00f8rn Dueholm","raw_affiliation_strings":["Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002939990","display_name":"Ditte Goldschmidt Jepsen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Ditte Goldschmidt Jepsen","raw_affiliation_strings":["Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035396060","display_name":"Niels Agerbirk","orcid":"https://orcid.org/0000-0003-4670-8330","observed_orcids":["https://orcid.org/0000-0003-4670-8330"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Niels Agerbirk","raw_affiliation_strings":["Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark"],"raw_orcid":"https://orcid.org/0000-0003-4670-8330","affiliations":[{"raw_affiliation_string":"Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5065188924","display_name":"Fernando Geu\u2010Flores","orcid":"https://orcid.org/0000-0002-5735-9810","observed_orcids":["https://orcid.org/0000-0002-5735-9810"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":true,"raw_author_name":"Fernando Geu-Flores","raw_affiliation_strings":["Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark. feg@plen.ku.dk","Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark"],"raw_orcid":"https://orcid.org/0000-0002-5735-9810","affiliations":[{"raw_affiliation_string":"Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark. feg@plen.ku.dk","institution_ids":["https://openalex.org/I124055696"]},{"raw_affiliation_string":"Section for Plant Biochemistry and Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark","institution_ids":["https://openalex.org/I124055696"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5065188924"],"corresponding_institution_ids":["https://openalex.org/I124055696"],"apc_list":{"value":2490,"currency":"USD","value_usd":2490},"apc_paid":{"value":2490,"currency":"USD","value_usd":2490},"fwci":1.0118,"has_fulltext":true,"cited_by_count":12,"citation_normalized_percentile":{"value":0.72461663,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":97},"biblio":{"volume":"17","issue":"1","first_page":"131","last_page":"131"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.8759999871253967,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.8759999871253967,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13506","display_name":"Botanical Research and Chemistry","score":0.04650000110268593,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.015200000256299973,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/virus-induced-gene-silencing","display_name":"virus-induced gene silencing","score":0.9610000252723694},{"id":"https://openalex.org/keywords/lupinus-angustifolius","display_name":"Lupinus angustifolius","score":0.9980000257492065},{"id":"https://openalex.org/keywords/apple-latent-spherical-virus","display_name":"apple latent spherical virus","score":0.9980000257492065},{"id":"https://openalex.org/keywords/gene-function-validation","display_name":"gene function validation","score":0.7409999966621399},{"id":"https://openalex.org/keywords/phytoene-desaturase","display_name":"phytoene desaturase","score":0.9210000038146973},{"id":"https://openalex.org/keywords/lysine-decarboxylase","display_name":"lysine decarboxylase","score":0.9769999980926514},{"id":"https://openalex.org/keywords/alkaloid-biosynthesis","display_name":"alkaloid biosynthesis","score":0.8389999866485596},{"id":"https://openalex.org/keywords/plant-virus-vectors","display_name":"plant virus vectors","score":0.3479999899864197}],"concepts":[{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.7407882809638977},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6831837892532349},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6284868717193604},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.552394449710846},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5073272585868835},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.47300654649734497},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3901163935661316},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.3230021595954895},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3227740228176117}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.1186/s13007-021-00832-4","doi":"https://doi.org/10.1186/s13007-021-00832-4","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13007-021-00832-4","pdf_url":"https://link.springer.com/content/pdf/10.1186/s13007-021-00832-4.pdf","source":{"id":"https://openalex.org/S184470774","display_name":"Plant Methods","issn_l":"1746-4811","issn":["1746-4811"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Methods","raw_type":"journal-article"},{"id":"pmid:34963500","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34963500","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant methods","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8714437","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8714437/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Methods","raw_type":"Text"},{"id":"pmh:oai:pure.atira.dk:openaire/fd46f257-cc52-40fb-b1a7-d31f20305c97","doi":null,"is_oa":true,"landing_page_url":"https://researchprofiles.ku.dk/da/publications/fd46f257-cc52-40fb-b1a7-d31f20305c97","pdf_url":null,"source":{"id":"https://openalex.org/S4306401983","display_name":"Research at the University of Copenhagen (University of Copenhagen)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I124055696","host_organization_name":"University of Copenhagen","host_organization_lineage":["https://openalex.org/I124055696"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mancinotti, D, Rodriguez, M C, Frick, K M, Dueholm, B, Jepsen, D G, Agerbirk, N & Geu-Flores, F 2021, 'Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin', Plant Methods, vol. 17, no. 1, 131. https://doi.org/10.1186/s13007-021-00832-4","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:doaj.org/article:a147fa13151147c48bd61748ecfb7070","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/a147fa13151147c48bd61748ecfb7070","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Plant Methods, Vol 17, Iss 1, Pp 1-13 (2021)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1186/s13007-021-00832-4","doi":"https://doi.org/10.1186/s13007-021-00832-4","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13007-021-00832-4","pdf_url":"https://link.springer.com/content/pdf/10.1186/s13007-021-00832-4.pdf","source":{"id":"https://openalex.org/S184470774","display_name":"Plant Methods","issn_l":"1746-4811","issn":["1746-4811"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Methods","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.9208999872207642,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2115677778","display_name":"Unleashing the potential of narrow leaf lupin as locally grown protein crop","funder_award_id":"846089","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G434337789","display_name":null,"funder_award_id":"HORIZON2020","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G5373911530","display_name":null,"funder_award_id":"NNF17OC0027744","funder_id":"https://openalex.org/F4320325957","funder_display_name":"Novo Nordisk Fonden"},{"id":"https://openalex.org/G6042272430","display_name":"Unleashing the potential of narrow leaf lupin as locally grown protein crop","funder_award_id":"846089","funder_id":"https://openalex.org/F4320338337","funder_display_name":"H2020 Marie Sk\u0142odowska-Curie Actions"},{"id":"https://openalex.org/G6835910553","display_name":null,"funder_award_id":"00015476","funder_id":"https://openalex.org/F4320310490","funder_display_name":"Villum Fonden"},{"id":"https://openalex.org/G8082347233","display_name":null,"funder_award_id":"15476","funder_id":"https://openalex.org/F4320310490","funder_display_name":"Villum Fonden"}],"funders":[{"id":"https://openalex.org/F4320310490","display_name":"Villum Fonden","ror":"https://ror.org/007ww2d15"},{"id":"https://openalex.org/F4320320300","display_name":"European Commission","ror":"https://ror.org/00k4n6c32"},{"id":"https://openalex.org/F4320322436","display_name":"Novo Nordisk","ror":"https://ror.org/0435rc536"},{"id":"https://openalex.org/F4320325957","display_name":"Novo Nordisk Fonden","ror":"https://ror.org/04txyc737"},{"id":"https://openalex.org/F4320338337","display_name":"H2020 Marie Sk\u0142odowska-Curie Actions","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4200317249.pdf","grobid_xml":"https://content.openalex.org/works/W4200317249.grobid-xml"},"referenced_works_count":51,"referenced_works":["https://openalex.org/W121482068","https://openalex.org/W1031098587","https://openalex.org/W1516440098","https://openalex.org/W1528019255","https://openalex.org/W1603807214","https://openalex.org/W1728204605","https://openalex.org/W1982348042","https://openalex.org/W1988099662","https://openalex.org/W1989454102","https://openalex.org/W1990100361","https://openalex.org/W2005256746","https://openalex.org/W2012111825","https://openalex.org/W2022187444","https://openalex.org/W2030188535","https://openalex.org/W2051807670","https://openalex.org/W2053384040","https://openalex.org/W2053899457","https://openalex.org/W2060254584","https://openalex.org/W2061383296","https://openalex.org/W2066147974","https://openalex.org/W2107925322","https://openalex.org/W2113987272","https://openalex.org/W2127936340","https://openalex.org/W2134126554","https://openalex.org/W2142330308","https://openalex.org/W2146186017","https://openalex.org/W2149406735","https://openalex.org/W2160643782","https://openalex.org/W2161645265","https://openalex.org/W2193801262","https://openalex.org/W2256783701","https://openalex.org/W2290490144","https://openalex.org/W2341088422","https://openalex.org/W2508114195","https://openalex.org/W2610352213","https://openalex.org/W2770517705","https://openalex.org/W2791481251","https://openalex.org/W2797491950","https://openalex.org/W2901726215","https://openalex.org/W2907162355","https://openalex.org/W2914153087","https://openalex.org/W2916007723","https://openalex.org/W2963694792","https://openalex.org/W2969104248","https://openalex.org/W2987920690","https://openalex.org/W2999807572","https://openalex.org/W3002915464","https://openalex.org/W3097316224","https://openalex.org/W3176696852","https://openalex.org/W3193107924","https://openalex.org/W4291567049"],"related_works":["https://openalex.org/W1031098587","https://openalex.org/W6957755725","https://openalex.org/W6902170396","https://openalex.org/W2060254584","https://openalex.org/W4281771012","https://openalex.org/W3035873587","https://openalex.org/W46791330","https://openalex.org/W2131287065","https://openalex.org/W2991615356","https://openalex.org/W2213922639"],"abstract_inverted_index":{"BACKGROUND:":[0],"Lupins":[1],"are":[2],"promising":[3],"protein":[4],"crops":[5],"with":[6],"an":[7,51,79],"increasing":[8],"amount":[9],"of":[10,23,45,102,116,141,163,188,243,253,283],"genomic":[11],"and":[12,37,109,199,202,266,281],"transcriptomic":[13],"resources.":[14],"The":[15,181],"new":[16],"resources":[17],"facilitate":[18],"the":[19,41,68,106,124,161,185,206,241,279],"in":[20,42,63,133,156,176,219,227,246,256,259,274],"silico":[21],"identification":[22,187],"candidate":[24],"genes":[25,62],"controlling":[26],"important":[27],"agronomic":[28],"traits.":[29],"However,":[30],"a":[31,89,169,177,220,264],"major":[32],"bottleneck":[33],"for":[34,54,99,110,270],"lupin":[35,65],"research":[36,280],"crop":[38],"improvement":[39,282],"is":[40,192,203,210],"planta":[43],"characterization":[44],"gene":[46,56,103,127,151,171,272],"function.":[47],"Here,":[48],"we":[49,122],"present":[50],"efficient":[52,100,267],"protocol":[53],"virus-induced":[55],"silencing":[57,136,147,191,242],"(VIGS)":[58],"to":[59,84,97,153,167],"down-regulate":[60],"endogenous":[61],"narrow-leafed":[64],"(NLL)":[66],"using":[67],"apple":[69],"latent":[70],"spherical":[71],"virus":[72],"(ALSV).":[73],"RESULTS:":[74],"We":[75,92,262],"identified":[76],"ALSV":[77,95],"as":[78,137],"appropriate":[80],"VIGS":[81,165,268],"vector":[82],"able":[83],"infect":[85],"NLL":[86],"without":[87],"causing":[88],"discernible":[90],"phenotype.":[91],"created":[93],"improved":[94],"vectors":[96],"allow":[98],"cloning":[101],"fragments":[104],"into":[105],"viral":[107,112],"genome":[108],"easier":[111],"propagation":[113],"via":[114],"agroinfiltration":[115],"Nicotiana":[117],"benthamiana.":[118],"Using":[119],"this":[120,284],"system,":[121],"silenced":[123,235],"visual":[125,186],"marker":[126],"phytoene":[128],"desaturase":[129],"(PDS),":[130],"which":[131,195],"resulted":[132,218,245],"systemic,":[134],"homogenous":[135],"indicated":[138],"by":[139,146,213],"bleaching":[140],"newly":[142],"produced":[143],"tissues.":[144],"Furthermore,":[145],"lysine":[148],"decarboxylase":[149],"(LaLDC)-a":[150],"likely":[152],"be":[154],"involved":[155],"toxic":[157],"alkaloid":[158,248,257],"biosynthesis-we":[159],"demonstrate":[160],"applicability":[162],"our":[164],"method":[166,269],"silence":[168],"target":[170],"alone":[172,236],"or":[173,223,237],"alongside":[174,238],"PDS":[175,214],"'PDS":[178],"co-silencing'":[179],"approach.":[180],"co-silencing":[182],"approach":[183],"allows":[184],"tissues":[189],"where":[190,205],"actively":[193],"occurring,":[194],"eases":[196],"tissue":[197],"harvesting":[198],"downstream":[200],"analysis,":[201],"useful":[204],"trait":[207],"under":[208],"study":[209],"not":[211],"affected":[212],"silencing.":[215],"Silencing":[216],"LaLDC":[217,233,244],"~":[221,224],"61%":[222],"67%":[225],"decrease":[226],"transcript":[228],"level,":[229],"depending":[230],"on":[231],"whether":[232],"was":[234],"PDS.":[239],"Overall,":[240],"reduced":[247],"levels,":[249],"providing":[250],"direct":[251],"evidence":[252],"its":[254],"involvement":[255],"biosynthesis":[258],"NLL.":[260,275],"CONCLUSIONS:":[261],"provide":[263],"rapid":[265],"validating":[271],"function":[273],"This":[276],"will":[277],"accelerate":[278],"underutilized":[285],"crop.":[286]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2021-12-31T00:00:00"}