{"id":"https://openalex.org/W4200220891","doi":"https://doi.org/10.1093/ornithology/ukab075","title":"Evaluating the impacts of metabarcoding primer selection on DNA characterization of diet in an aerial insectivore, the Purple Martin","display_name":"Evaluating the impacts of metabarcoding primer selection on DNA characterization of diet in an aerial insectivore, the Purple Martin","publication_year":2021,"publication_date":"2021-12-03","ids":{"openalex":"https://openalex.org/W4200220891","doi":"https://doi.org/10.1093/ornithology/ukab075"},"language":"en","primary_location":{"id":"doi:10.1093/ornithology/ukab075","doi":"https://doi.org/10.1093/ornithology/ukab075","is_oa":false,"landing_page_url":"https://doi.org/10.1093/ornithology/ukab075","pdf_url":null,"source":{"id":"https://openalex.org/S4210233486","display_name":"The Auk","issn_l":"2732-4613","issn":["2732-4613"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["jufo-1","russia-white-list-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Ornithology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5054185834","display_name":"Anna Forsman","orcid":"https://orcid.org/0000-0002-5942-4671","observed_orcids":["https://orcid.org/0000-0002-5942-4671"]},"institutions":[{"id":"https://openalex.org/I106165777","display_name":"University of Central Florida","ror":"https://ror.org/036nfer12","country_code":"US","type":"education","lineage":["https://openalex.org/I106165777"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Anna M Forsman","raw_affiliation_strings":["Department of Biology, University of Central Florida, Orlando, Florida, USA","Genomics & Bioinformatics Cluster, University of Central Florida, Orlando, Florida, USA"],"raw_orcid":"https://orcid.org/0000-0002-5942-4671","affiliations":[{"raw_affiliation_string":"Department of Biology, University of Central Florida, Orlando, Florida, USA","institution_ids":["https://openalex.org/I106165777"]},{"raw_affiliation_string":"Genomics & Bioinformatics Cluster, University of Central Florida, Orlando, Florida, USA","institution_ids":["https://openalex.org/I106165777"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016383781","display_name":"Brandon D. Hoenig","orcid":"https://orcid.org/0000-0001-6344-3202","observed_orcids":["https://orcid.org/0000-0001-6344-3202"]},"institutions":[{"id":"https://openalex.org/I165102784","display_name":"Duquesne University","ror":"https://ror.org/02336z538","country_code":"US","type":"education","lineage":["https://openalex.org/I165102784"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brandon D Hoenig","raw_affiliation_strings":["Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania, USA","institution_ids":["https://openalex.org/I165102784"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027302470","display_name":"Stephanie A Gaspar","orcid":"https://orcid.org/0000-0002-5055-1199","observed_orcids":["https://orcid.org/0000-0002-5055-1199"]},"institutions":[{"id":"https://openalex.org/I106165777","display_name":"University of Central Florida","ror":"https://ror.org/036nfer12","country_code":"US","type":"education","lineage":["https://openalex.org/I106165777"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephanie A Gaspar","raw_affiliation_strings":["Department of Biology, University of Central Florida, Orlando, Florida, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Central Florida, Orlando, Florida, USA","institution_ids":["https://openalex.org/I106165777"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052767166","display_name":"Jason D. Fischer","orcid":"https://orcid.org/0000-0002-2997-2933","observed_orcids":["https://orcid.org/0000-0002-2997-2933"]},"institutions":[{"id":"https://openalex.org/I4210142140","display_name":"Walt Disney (United States)","ror":"https://ror.org/04eg47h42","country_code":"US","type":"company","lineage":["https://openalex.org/I4210142140"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jason D Fischer","raw_affiliation_strings":["Disney\u2019s Animals, Science and Environment, Lake Buena Vista, Florida, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Disney\u2019s Animals, Science and Environment, Lake Buena Vista, Florida, USA","institution_ids":["https://openalex.org/I4210142140"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033500983","display_name":"Joe Siegrist","orcid":"https://orcid.org/0000-0002-4381-0623","observed_orcids":["https://orcid.org/0000-0002-4381-0623"]},"institutions":[{"id":"https://openalex.org/I4210162342","display_name":"Purple Martin Conservation Association","ror":"https://ror.org/05amca937","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210162342"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joe Siegrist","raw_affiliation_strings":["Purple Martin Conservation Association, Erie, Pennsylvania, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Purple Martin Conservation Association, Erie, Pennsylvania, USA","institution_ids":["https://openalex.org/I4210162342"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5012246400","display_name":"Kevin C. Fraser","orcid":"https://orcid.org/0000-0002-0308-716X","observed_orcids":["https://orcid.org/0000-0002-0308-716X"]},"institutions":[{"id":"https://openalex.org/I46247651","display_name":"University of Manitoba","ror":"https://ror.org/02gfys938","country_code":"CA","type":"education","lineage":["https://openalex.org/I46247651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Kevin Fraser","raw_affiliation_strings":["Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada","institution_ids":["https://openalex.org/I46247651"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5054185834"],"corresponding_institution_ids":["https://openalex.org/I106165777"],"apc_list":{"value":3194,"currency":"USD","value_usd":3194},"apc_paid":null,"fwci":3.4347,"has_fulltext":false,"cited_by_count":41,"citation_normalized_percentile":{"value":0.93461281,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"139","issue":"1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12388","display_name":"Identification and Quantification in Food","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12073","display_name":"Isotope Analysis in Ecology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-metabarcoding","display_name":"DNA metabarcoding","score":0.9620000123977661},{"id":"https://openalex.org/keywords/primer-selection","display_name":"primer selection","score":0.9079999923706055},{"id":"https://openalex.org/keywords/purple-martin","display_name":"purple martin","score":0.7559999823570251},{"id":"https://openalex.org/keywords/arthropod-prey","display_name":"arthropod prey","score":0.7379999756813049},{"id":"https://openalex.org/keywords/fecal-samples","display_name":"fecal samples","score":0.6639999747276306},{"id":"https://openalex.org/keywords/taxonomic-bias","display_name":"taxonomic bias","score":0.6899999976158142},{"id":"https://openalex.org/keywords/dietary-variation","display_name":"diet variation","score":0.503000020980835},{"id":"https://openalex.org/keywords/alpha-and-beta-diversity","display_name":"alpha and beta diversity","score":0.7509999871253967},{"id":"https://openalex.org/keywords/feeding-ecology","display_name":"feeding ecology","score":0.7269999980926514}],"concepts":[{"id":"https://openalex.org/C175406946","wikidata":"https://www.wikidata.org/wiki/Q677088","display_name":"Insectivore","level":3,"score":0.9055938720703125},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.7785074710845947},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7478690147399902},{"id":"https://openalex.org/C165287380","wikidata":"https://www.wikidata.org/wiki/Q2916569","display_name":"Foraging","level":2,"score":0.5961849689483643},{"id":"https://openalex.org/C90856448","wikidata":"https://www.wikidata.org/wiki/Q431","display_name":"Zoology","level":1,"score":0.5224239230155945},{"id":"https://openalex.org/C188382862","wikidata":"https://www.wikidata.org/wiki/Q170430","display_name":"Predation","level":2,"score":0.472608357667923},{"id":"https://openalex.org/C81917197","wikidata":"https://www.wikidata.org/wiki/Q628760","display_name":"Selection (genetic algorithm)","level":2,"score":0.4695573151111603},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.4159519076347351},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1093/ornithology/ukab075","doi":"https://doi.org/10.1093/ornithology/ukab075","is_oa":false,"landing_page_url":"https://doi.org/10.1093/ornithology/ukab075","pdf_url":null,"source":{"id":"https://openalex.org/S4210233486","display_name":"The Auk","issn_l":"2732-4613","issn":["2732-4613"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["jufo-1","russia-white-list-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Ornithology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310161","display_name":"University of Central Florida","ror":"https://ror.org/036nfer12"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":69,"referenced_works":["https://openalex.org/W1494969944","https://openalex.org/W1571552535","https://openalex.org/W1994022238","https://openalex.org/W1997125947","https://openalex.org/W2006282417","https://openalex.org/W2008871895","https://openalex.org/W2027573634","https://openalex.org/W2037977039","https://openalex.org/W2051331361","https://openalex.org/W2051569068","https://openalex.org/W2065838202","https://openalex.org/W2096488278","https://openalex.org/W2099901798","https://openalex.org/W2102408656","https://openalex.org/W2103739720","https://openalex.org/W2106778108","https://openalex.org/W2114259093","https://openalex.org/W2115853379","https://openalex.org/W2123119542","https://openalex.org/W2131894360","https://openalex.org/W2149415648","https://openalex.org/W2159858445","https://openalex.org/W2171783890","https://openalex.org/W2172820226","https://openalex.org/W2175688155","https://openalex.org/W2234437295","https://openalex.org/W2262375083","https://openalex.org/W2329198233","https://openalex.org/W2371344252","https://openalex.org/W2401404581","https://openalex.org/W2460850757","https://openalex.org/W2507473831","https://openalex.org/W2530132666","https://openalex.org/W2556227571","https://openalex.org/W2582743722","https://openalex.org/W2592367925","https://openalex.org/W2724989966","https://openalex.org/W2748110867","https://openalex.org/W2766208183","https://openalex.org/W2803287262","https://openalex.org/W2810540820","https://openalex.org/W2858246490","https://openalex.org/W2888451776","https://openalex.org/W2898359744","https://openalex.org/W2898547558","https://openalex.org/W2910984889","https://openalex.org/W2923297262","https://openalex.org/W2952734356","https://openalex.org/W2963276645","https://openalex.org/W2963928659","https://openalex.org/W2966260219","https://openalex.org/W2968380997","https://openalex.org/W2974449448","https://openalex.org/W3004940541","https://openalex.org/W3007666433","https://openalex.org/W3024064015","https://openalex.org/W3026163996","https://openalex.org/W3044127477","https://openalex.org/W3046526526","https://openalex.org/W3088141516","https://openalex.org/W3089811728","https://openalex.org/W3105400276","https://openalex.org/W3113240106","https://openalex.org/W3121953277","https://openalex.org/W4200297812","https://openalex.org/W4213027992","https://openalex.org/W4213130891","https://openalex.org/W4250963355","https://openalex.org/W6685423195"],"related_works":["https://openalex.org/W3137456990","https://openalex.org/W3026163996","https://openalex.org/W4285785954","https://openalex.org/W7081701179","https://openalex.org/W7213279843","https://openalex.org/W4229022292","https://openalex.org/W3139487233","https://openalex.org/W2903599381","https://openalex.org/W6948447762","https://openalex.org/W6929428519"],"abstract_inverted_index":{"Abstract":[0],"DNA":[1,40],"metabarcoding":[2,41,110,278],"is":[3,47,274],"a":[4],"molecular":[5],"technique":[6],"frequently":[7,38],"used":[8,39,95,121],"to":[9,20,96,150,157,203,213,216,283,291],"characterize":[10],"diet":[11,154,166,249],"composition":[12,155],"of":[13,32,67,111,165,206,234,248],"insectivorous":[14,80,98],"birds.":[15,99],"However,":[16,49],"results":[17,294],"are":[18],"sensitive":[19],"methodological":[21],"decisions":[22],"made":[23],"during":[24],"sample":[25],"processing,":[26],"with":[27,83,117],"primer":[28,42,72,105,123,160,240,264,300],"selection":[29,161],"being":[30],"one":[31],"the":[33,103,118,143,244,275,289],"most":[34,37,245],"critical.":[35],"The":[36],"set":[43,106,265],"for":[44,78,107,227],"avian":[45,112,285],"insectivores":[46],"ZBJ.":[48,199],"recent":[50],"studies":[51],"have":[52],"found":[53,222,261],"that":[54,62,223,237],"ZBJ":[55,122,219,226,299],"produces":[56],"significant":[57],"biases":[58,86],"in":[59,109,142,148,153,253,268],"prey":[60,176],"classification":[61],"likely":[63],"influence":[64],"our":[65,163,271],"understanding":[66,164],"foraging":[68],"ecology.":[69],"A":[70],"new":[71],"set,":[73],"ANML,":[74,191],"has":[75,91],"shown":[76],"promise":[77],"characterizing":[79],"bat":[81],"diets":[82,114],"fewer":[84],"taxonomic":[85,230],"than":[87],"ZBJ,":[88],"but":[89,236],"ANML":[90,104,211,224,281,297],"not":[92],"yet":[93],"been":[94],"study":[97,279],"Here,":[100],"we":[101,172],"evaluate":[102],"use":[108],"insectivore":[113],"through":[115],"comparison":[116],"more":[119],"commonly":[120],"set.":[124],"Fecal":[125],"samples":[126],"were":[127,187,195,201],"collected":[128],"from":[129,210,218],"both":[130,239,254],"adult":[131],"and":[132,145,156,180,232,256,267,287,298],"nestling":[133],"Purple":[134],"Martins":[135],"(Progne":[136],"subis":[137],"subis)":[138],"at":[139],"2":[140],"sites":[141,259],"USA":[144],"1":[146],"site":[147],"Canada":[149],"maximize":[151],"variation":[152,167,252],"determine":[158],"if":[159],"impacts":[162],"among":[168],"sites.":[169],"In":[170],"total,":[171],"detected":[173,189,197],"71":[174],"arthropod":[175],"species,":[177],"39":[178],"families,":[179],"10":[181],"orders.":[182],"Of":[183],"these,":[184],"40":[185],"species":[186,214],"uniquely":[188,196],"by":[190,198,296],"whereas":[192],"only":[193],"11":[194],"We":[200,221],"able":[202],"classify":[204],"54.8%":[205],"exact":[207],"sequence":[208],"variants":[209],"libraries":[212],"compared":[215],"33.3%":[217],"libraries.":[220],"outperformed":[225],"PCR":[228],"efficacy,":[229],"coverage,":[231],"specificity":[233],"classification,":[235],"using":[238,262,280],"sets":[241],"together":[242],"produced":[243],"comprehensive":[246],"characterizations":[247],"composition.":[250],"Significant":[251],"alpha-":[255],"beta-diversity":[257],"between":[258],"was":[260],"each":[263],"separately":[266],"combination.":[269],"To":[270],"knowledge,":[272],"this":[273],"first":[276,290],"published":[277],"primers":[282],"describe":[284],"diet,":[286],"also":[288],"directly":[292],"compare":[293],"returned":[295],"sets.":[301]},"counts_by_year":[{"year":2026,"cited_by_count":7},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":10},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":9},{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2021-12-31T00:00:00"}