{"id":"https://openalex.org/W40793414","doi":"https://doi.org/10.2460/ajvr.1991.52.06.880","title":"Replication of parainfluenza type-3 virus and bovine respiratory syncytial virus in isolated bovine type-II alveolar epithelial cells","display_name":"Replication of parainfluenza type-3 virus and bovine respiratory syncytial virus in isolated bovine type-II alveolar epithelial cells","publication_year":1991,"publication_date":"1991-06-01","ids":{"openalex":"https://openalex.org/W40793414","doi":"https://doi.org/10.2460/ajvr.1991.52.06.880","mag":"40793414","pmid":"https://pubmed.ncbi.nlm.nih.gov/1652906"},"language":"en","primary_location":{"id":"doi:10.2460/ajvr.1991.52.06.880","doi":"https://doi.org/10.2460/ajvr.1991.52.06.880","is_oa":true,"landing_page_url":"https://doi.org/10.2460/ajvr.1991.52.06.880","pdf_url":"https://avmajournals.avma.org/downloadpdf/view/journals/ajvr/52/6/ajvr.1991.52.06.880.pdf","source":{"id":"https://openalex.org/S196975874","display_name":"American Journal of Veterinary Research","issn_l":"0002-9645","issn":["0002-9645","1943-5681"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-2","medline","norway-1","oarl","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4322545313","host_organization_name":"American Veterinary Medical Association","host_organization_lineage":["https://openalex.org/P4322545313"],"host_organization_lineage_names":["American Veterinary Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Veterinary Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://avmajournals.avma.org/downloadpdf/view/journals/ajvr/52/6/ajvr.1991.52.06.880.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046012473","display_name":"William L. Castleman","orcid":"https://orcid.org/0000-0002-2868-346X","observed_orcids":["https://orcid.org/0000-0002-2868-346X"]},"institutions":[{"id":"https://openalex.org/I1304256225","display_name":"University of Wisconsin System","ror":"https://ror.org/03ydkyb10","country_code":"US","type":"education","lineage":["https://openalex.org/I1304256225"]},{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"W. L. Castleman","raw_affiliation_strings":["From the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Dr West, Madison, WI 53706"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Dr West, Madison, WI 53706","institution_ids":["https://openalex.org/I1304256225","https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041859055","display_name":"P. J. Northrop","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1304256225","display_name":"University of Wisconsin System","ror":"https://ror.org/03ydkyb10","country_code":"US","type":"education","lineage":["https://openalex.org/I1304256225"]},{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P. J. Northrop","raw_affiliation_strings":["From the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Dr West, Madison, WI 53706"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Dr West, Madison, WI 53706","institution_ids":["https://openalex.org/I1304256225","https://openalex.org/I135310074"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5012339879","display_name":"Pamela K. McAllister","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1304256225","display_name":"University of Wisconsin System","ror":"https://ror.org/03ydkyb10","country_code":"US","type":"education","lineage":["https://openalex.org/I1304256225"]},{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P. K. McAllister","raw_affiliation_strings":["From the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Dr West, Madison, WI 53706"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Dr West, Madison, WI 53706","institution_ids":["https://openalex.org/I1304256225","https://openalex.org/I135310074"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":9,"citation_normalized_percentile":{"value":0.00547558,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"52","issue":"6","first_page":"880","last_page":"885"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11243","display_name":"Respiratory viral infections research","score":0.8003000020980835,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11243","display_name":"Respiratory viral infections research","score":0.8003000020980835,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.12780000269412994,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.040699999779462814,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bovine-respiratory-syncytial-virus","display_name":"bovine respiratory syncytial virus","score":1.0},{"id":"https://openalex.org/keywords/viral-replication","display_name":"viral replication","score":0.8410000205039978},{"id":"https://openalex.org/keywords/cell-isolation","display_name":"cell isolation","score":0.7120000123977661},{"id":"https://openalex.org/keywords/cell-culture","display_name":"cell culture","score":0.6060000061988831},{"id":"https://openalex.org/keywords/alveolar-epithelium","display_name":"alveolar epithelium","score":0.6060000061988831},{"id":"https://openalex.org/keywords/calves","display_name":"calves","score":0.4449999928474426},{"id":"https://openalex.org/keywords/respiratory-syncytial-virus","display_name":"respiratory syncytial virus","score":0.5379999876022339}],"concepts":[{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.7261180877685547},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.6220002174377441},{"id":"https://openalex.org/C534529494","wikidata":"https://www.wikidata.org/wiki/Q7891","display_name":"Respiratory system","level":2,"score":0.4985477924346924},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4648389518260956},{"id":"https://openalex.org/C2780813555","wikidata":"https://www.wikidata.org/wiki/Q911129","display_name":"Paramyxoviridae","level":4,"score":0.45109790563583374},{"id":"https://openalex.org/C12590798","wikidata":"https://www.wikidata.org/wiki/Q3933199","display_name":"Replication (statistics)","level":2,"score":0.4114507734775543},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3460777997970581},{"id":"https://openalex.org/C2780727368","wikidata":"https://www.wikidata.org/wiki/Q1928978","display_name":"Viral disease","level":3,"score":0.12142211198806763},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002499","descriptor_name":"Centrifugation, Density Gradient","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004848","descriptor_name":"Epithelium","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D004848","descriptor_name":"Epithelium","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D005374","descriptor_name":"Filtration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007074","descriptor_name":"Immunoglobulin G","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010224","descriptor_name":"Parainfluenza Virus 3, Human","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D010224","descriptor_name":"Parainfluenza Virus 3, Human","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D011650","descriptor_name":"Pulmonary Alveoli","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D011650","descriptor_name":"Pulmonary Alveoli","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D011650","descriptor_name":"Pulmonary Alveoli","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D012136","descriptor_name":"Respiratory Syncytial Viruses","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D012136","descriptor_name":"Respiratory Syncytial Viruses","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D014779","descriptor_name":"Virus Replication","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D037102","descriptor_name":"Lectins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.2460/ajvr.1991.52.06.880","doi":"https://doi.org/10.2460/ajvr.1991.52.06.880","is_oa":true,"landing_page_url":"https://doi.org/10.2460/ajvr.1991.52.06.880","pdf_url":"https://avmajournals.avma.org/downloadpdf/view/journals/ajvr/52/6/ajvr.1991.52.06.880.pdf","source":{"id":"https://openalex.org/S196975874","display_name":"American Journal of Veterinary Research","issn_l":"0002-9645","issn":["0002-9645","1943-5681"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-2","medline","norway-1","oarl","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4322545313","host_organization_name":"American Veterinary Medical Association","host_organization_lineage":["https://openalex.org/P4322545313"],"host_organization_lineage_names":["American Veterinary Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Veterinary Research","raw_type":"journal-article"},{"id":"pmid:1652906","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1652906","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American journal of veterinary research","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.2460/ajvr.1991.52.06.880","doi":"https://doi.org/10.2460/ajvr.1991.52.06.880","is_oa":true,"landing_page_url":"https://doi.org/10.2460/ajvr.1991.52.06.880","pdf_url":"https://avmajournals.avma.org/downloadpdf/view/journals/ajvr/52/6/ajvr.1991.52.06.880.pdf","source":{"id":"https://openalex.org/S196975874","display_name":"American Journal of Veterinary Research","issn_l":"0002-9645","issn":["0002-9645","1943-5681"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-2","medline","norway-1","oarl","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4322545313","host_organization_name":"American Veterinary Medical Association","host_organization_lineage":["https://openalex.org/P4322545313"],"host_organization_lineage_names":["American Veterinary Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Veterinary Research","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.504800021648407,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W40793414.pdf","grobid_xml":"https://content.openalex.org/works/W40793414.grobid-xml"},"referenced_works_count":30,"referenced_works":["https://openalex.org/W15112479","https://openalex.org/W72079153","https://openalex.org/W1410463955","https://openalex.org/W1417746342","https://openalex.org/W1434937197","https://openalex.org/W1493924809","https://openalex.org/W1847592245","https://openalex.org/W1911192242","https://openalex.org/W1919840375","https://openalex.org/W1924167294","https://openalex.org/W1949178966","https://openalex.org/W1954428203","https://openalex.org/W1970385846","https://openalex.org/W1994908414","https://openalex.org/W2021989778","https://openalex.org/W2023253493","https://openalex.org/W2024107440","https://openalex.org/W2028869335","https://openalex.org/W2032341993","https://openalex.org/W2072485020","https://openalex.org/W2076945592","https://openalex.org/W2114930069","https://openalex.org/W2178389233","https://openalex.org/W2389739558","https://openalex.org/W2408188831","https://openalex.org/W2415123690","https://openalex.org/W2424384909","https://openalex.org/W4298363866","https://openalex.org/W4407210155","https://openalex.org/W4409121920"],"related_works":["https://openalex.org/W1410463955","https://openalex.org/W1847592245","https://openalex.org/W2922478799","https://openalex.org/W2885175330","https://openalex.org/W2385797627","https://openalex.org/W2098598301","https://openalex.org/W2403819019","https://openalex.org/W3155757136","https://openalex.org/W2056357276","https://openalex.org/W1909340305"],"abstract_inverted_index":{"The":[0],"objectives":[1],"of":[2,41,59,69,77,82,128,144,159,169],"our":[3],"research":[4],"were":[5,55,91,108,137],"to":[6,27,61],"determine":[7,28],"whether":[8,29],"bovine":[9,19,31,42,47,145,150],"pulmonary":[10,70],"type-II":[11,32,105,118,129,173],"alveolar":[12,33,52,106,121,174],"epithelial":[13,34,53,175],"cells":[14,35,54,78,107,119,176],"could":[15],"be":[16],"isolated":[17,30,56],"from":[18,57,162],"lung":[20],"and":[21,26,46,74,84,100,123,140,149,166],"maintained":[22,109,124],"in":[23,172],"tissue":[24,71,113],"culture":[25],"would":[36],"support":[37],"productive":[38,142],"viral":[39,170],"replication":[40,143],"parainfluenza":[43,146],"type-3":[44,147],"virus":[45,148],"respiratory":[48,151],"syncytial":[49,152],"virus.":[50,153],"Type-II":[51],"lungs":[58],"4-":[60],"7-day-old":[62],"male":[63],"Holstein":[64],"calves":[65],"by":[66,75,94,101,157,167,177],"enzymatic":[67],"dissociation":[68],"with":[72,79,139],"trypsin":[73],"separation":[76],"the":[80],"use":[81],"filtration":[83],"centrifugation":[85],"on":[86,96,110],"continuous":[87],"Percoll":[88],"gradients.":[89],"Cells":[90],"further":[92],"separated":[93],"panning":[95],"IgG-coated":[97],"plastic":[98],"plates":[99],"lectin":[102],"binding.":[103],"Isolated":[104],"basement":[111],"membrane-coated":[112],"cultured":[114],"plates.":[115],"In":[116],"culture,":[117],"formed":[120],"structures":[122,171],"other":[125],"cytologic":[126],"features":[127],"cells,":[130],"including":[131],"osmiophilic":[132],"lamellar":[133],"inclusions.":[134],"Cell":[135],"cultures":[136,165],"inoculated":[138,163],"supported":[141],"This":[154],"was":[155],"determined":[156],"recovery":[158],"infectious":[160],"viruses":[161],"cell":[164],"identification":[168],"transmission":[178],"electron":[179],"microscopy.":[180]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}