{"id":"https://openalex.org/W40488589","doi":null,"title":"Evaluation of three rapid RNA extraction reagents: relevance for use in RT-PCR's and measurement of low level gene expression in clinical samples.","display_name":"Evaluation of three rapid RNA extraction reagents: relevance for use in RT-PCR's and measurement of low level gene expression in clinical samples.","publication_year":1997,"publication_date":"1997-12-01","ids":{"openalex":"https://openalex.org/W40488589","mag":"40488589","pmid":"https://pubmed.ncbi.nlm.nih.gov/9489949"},"language":"en","primary_location":{"id":"pmid:9489949","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9489949","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cellular and molecular biology (Noisy-le-Grand, France)","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046750108","display_name":"Tony Chadderton","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Chadderton","raw_affiliation_strings":["Northeastern Ontario Regional Cancer Centre, Sudbury, Ontario, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Northeastern Ontario Regional Cancer Centre, Sudbury, Ontario, Canada","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"C Wilson","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C Wilson","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042833263","display_name":"Mary A. Bewick","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Bewick","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5109150061","display_name":"Stefan Gl\u00fcck","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S Gl\u00fcck","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4961,"has_fulltext":false,"cited_by_count":60,"citation_normalized_percentile":{"value":0.56352367,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"43","issue":"8","first_page":"1227","last_page":"34"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11314","display_name":"Clinical Laboratory Practices and Quality Control","score":0.0,"subfield":{"id":"https://openalex.org/subfields/3607","display_name":"Medical Laboratory Technology"},"field":{"id":"https://openalex.org/fields/36","display_name":"Health Professions"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/whole-blood-rna","display_name":"whole blood RNA","score":0.5440000295639038},{"id":"https://openalex.org/keywords/peripheral-blood","display_name":"peripheral blood","score":0.8119999766349792},{"id":"https://openalex.org/keywords/bone-marrow","display_name":"bone marrow","score":0.9850000143051147},{"id":"https://openalex.org/keywords/mdr1-gene-expression","display_name":"MDR1 gene expression","score":0.7549999952316284},{"id":"https://openalex.org/keywords/mrp","display_name":"MRP","score":0.6930000185966492},{"id":"https://openalex.org/keywords/topoisomerase-ii-alpha","display_name":"TOP2A","score":0.9539999961853027},{"id":"https://openalex.org/keywords/multidrug-resistance","display_name":"multidrug resistance","score":0.7300000190734863},{"id":"https://openalex.org/keywords/heparin-interference","display_name":"heparin interference","score":0.6880000233650208},{"id":"https://openalex.org/keywords/multiplex-rt-pcr","display_name":"multiplex RT-PCR","score":0.7350000143051147},{"id":"https://openalex.org/keywords/breast-cancer","display_name":"breast cancer","score":0.6859999895095825}],"concepts":[{"id":"https://openalex.org/C205713591","wikidata":"https://www.wikidata.org/wiki/Q4463029","display_name":"Trizol","level":5,"score":0.9499896764755249},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.6191821098327637},{"id":"https://openalex.org/C112190416","wikidata":"https://www.wikidata.org/wiki/Q2124450","display_name":"RNA extraction","level":4,"score":0.6187268495559692},{"id":"https://openalex.org/C2778271344","wikidata":"https://www.wikidata.org/wiki/Q903395","display_name":"Agarose gel electrophoresis","level":3,"score":0.5109497904777527},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4761597812175751},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.47432607412338257},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4489818513393402},{"id":"https://openalex.org/C1621761","wikidata":"https://www.wikidata.org/wiki/Q7997549","display_name":"Whole blood","level":2,"score":0.441257506608963},{"id":"https://openalex.org/C47698986","wikidata":"https://www.wikidata.org/wiki/Q10018973","display_name":"18S ribosomal RNA","level":4,"score":0.4162823557853699},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.41608723998069763},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.32528460025787354},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.27563154697418213},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.2634354531764984},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2596450448036194},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.25877463817596436},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.17589139938354492},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14169150590896606}],"mesh":[{"descriptor_ui":"D005069","descriptor_name":"Evaluation Studies as Topic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011933","descriptor_name":"Reagent Kits, Diagnostic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:9489949","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9489949","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cellular and molecular biology (Noisy-le-Grand, France)","raw_type":"Journal Article"},{"id":"mag:40488589","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9489949","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2074620807","https://openalex.org/W3046439740","https://openalex.org/W2022801364","https://openalex.org/W2384667834","https://openalex.org/W2393106926","https://openalex.org/W2009763816","https://openalex.org/W1719391341","https://openalex.org/W7099881484","https://openalex.org/W4392409300","https://openalex.org/W2077320790"],"abstract_inverted_index":{"Although":[0],"peripheral":[1,125,245],"blood":[2,28,77,129,335,411],"and":[3,24,110,123,132,185,208,238,252,279,339,375,391],"bone":[4,127],"marrow":[5],"are":[6,17,342],"usually":[7],"readily":[8],"available":[9],"from":[10,72,80,243,306,333,380],"patients,":[11],"present":[12],"techniques":[13],"of":[14,21,26,45,75,118,121,158,174,195,203,266,378,389],"RNA":[15,31,71,175,201,242,247,253,320,331,379],"extraction":[16],"tedious,":[18],"require":[19],"millilitres":[20],"starting":[22],"material":[23,382],"removal":[25,44],"red":[27],"cells":[29],"before":[30],"purification.":[32,359],"Further,":[33],"successful":[34,318],"reverse":[35],"transcriptase":[36],"polymerase":[37],"chain":[38],"reaction":[39,263],"(RT-PCR)":[40],"amplification":[41,314],"requires":[42],"the":[43,51,81,96,107,172,205,213,224,250,267,273,301,330,337,340,364,386],"haemoglobin":[46],"derivatives":[47],"which":[48,177],"interfere":[49],"with":[50,92,219,249,319,322,336,409],"PCR":[52,295],"process.":[53],"Recently,":[54],"one":[55],"step":[56],"rapid":[57],"use":[58,84,152,345,408],"reagents":[59,112,255],"have":[60],"become":[61],"available,":[62],"claiming":[63],"to":[64,85,102,141,149,310,395],"be":[65,147,288],"useful":[66],"for":[67,264,344,406],"obtaining":[68,372],"high":[69],"quality":[70,173],"microlitre":[73],"quantities":[74],"whole":[76,292,334,410],"drawn":[78],"directly":[79],"patient.":[82],"Their":[83],"date":[86],"in":[87,95,139,153,171,259,290,346,371,393],"clinical":[88,119],"samples":[89,308],"appears":[90],"limited":[91],"little":[93],"information":[94],"literature":[97],"documented.":[98],"In":[99,325],"an":[100,154],"attempt":[101],"overcome":[103],"this,":[104],"we":[105,327],"tested":[106],"Trizol-LS,":[108],"RNA-STAT-50":[109,341],"Ultraspec-3":[111,239,323,397,401],"upon":[113],"a":[114,133,260,373],"statistically":[115,226],"significant":[116,227],"number":[117],"isolates":[120],"fresh":[122,244],"cryopreserved":[124],"blood,":[126],"marrow,":[128],"apheresis":[130],"products":[131,296],"breast":[134],"cancer":[135],"cell":[136],"line":[137],"(MCF7)":[138],"order":[140],"evaluate":[142],"whether":[143],"these":[144,196],"methods":[145,197],"could":[146,198,287],"applied":[148],"routine":[150],"laboratory":[151],"RT-PCR":[155,262,313],"method":[156],"capable":[157,202],"detecting":[159],"rare":[160,354],"gene":[161,285],"expression.":[162],"Our":[163,360],"findings":[164],"showed":[165,192],"that":[166,193,231,329,352,363],"there":[167],"was":[168,178,233,256,304,315,367],"some":[169],"variation":[170],"extracted":[176,248,321,332],"indicated":[179,230,362],"by":[180,385],"absorbance":[181],"spectrophotometry":[182],"at":[183,240],"260":[184],"280":[186],"nm.":[187],"1%":[188],"agarose":[189],"gel":[190],"electrophoresis":[191],"each":[194],"yield":[199],"total":[200],"generating":[204],"signature":[206],"18S":[207],"28S":[209],"rRNA":[210],"bands.":[211],"Using":[212],"Kruskal-Wallis":[214],"non-parametric":[215],"anova":[216],"test":[217],"combined":[218],"Dunn's":[220],"multiple":[221],"comparison":[222,394],"test,":[223],"only":[225,298],"difference":[228],"(p<0.05)":[229],"Trizol-LS":[232,251,338,365],"more":[234,369],"reliable":[235],"than":[236],"RNA-STAT-50-LS":[237,399],"extracting":[241],"blood.":[246,293],"STAT-50":[254],"successfully":[257],"amplified":[258],"multiplex":[261],"detection":[265],"multi-drug":[268,274],"resistance":[269,275],"related":[270,276],"genes":[271,356],"MDR1,":[272],"protein":[277],"(MRP)":[278],"topoisomerase":[280],"IIalpha.":[281],"Low":[282],"level":[283],"MDR1":[284],"expression":[286],"detected":[289],"frozen":[291],"However,":[294],"were":[297],"seen":[299],"when":[300],"anti-coagulant":[302],"heparin":[303],"removed":[305],"all":[307],"prior":[309],"cDNA":[311],"production.":[312],"not":[316,403],"100%":[317],"reagent.":[324],"conclusion,":[326],"found":[328],"suitable":[343],"clinically":[347],"relevant":[348],"molecular":[349],"biology":[350],"protocols":[351],"analyze":[353],"event":[355],"without":[357],"further":[358],"results":[361],"reagent":[366],"generally":[368],"consistent":[370],"pure":[374],"sufficient":[376],"quantity":[377,392],"patient":[381],"as":[383],"shown":[384],"mean":[387],"result":[388],"purity":[390],"either":[396],"or":[398],"reagents.":[400],"is":[402],"easily":[404],"suited":[405],"direct":[407],"products.":[412]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
