{"id":"https://openalex.org/W40430544","doi":null,"title":"Improved design of riboprobes from pBluescript and related vectors for in situ hybridization.","display_name":"Improved design of riboprobes from pBluescript and related vectors for in situ hybridization.","publication_year":1993,"publication_date":"1993-03-01","ids":{"openalex":"https://openalex.org/W40430544","mag":"40430544","pmid":"https://pubmed.ncbi.nlm.nih.gov/8457354"},"language":"en","primary_location":{"id":"pmid:8457354","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8457354","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5083373028","display_name":"Halina Witkiewicz","orcid":"https://orcid.org/0000-0001-5126-5015","observed_orcids":["https://orcid.org/0000-0001-5126-5015"]},"institutions":[{"id":"https://openalex.org/I1330342723","display_name":"Mayo Clinic","ror":"https://ror.org/02qp3tb03","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1330342723"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"H Witkiewicz","raw_affiliation_strings":["Department of Orthopedics, Mayo Clinic, Rochester, MN 55905"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, Mayo Clinic, Rochester, MN 55905","institution_ids":["https://openalex.org/I1330342723"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077089290","display_name":"Mark E. Bolander","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M E Bolander","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5050814031","display_name":"Dylan R. Edwards","orcid":"https://orcid.org/0000-0002-3292-2064","observed_orcids":["https://orcid.org/0000-0002-3292-2064"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D R Edwards","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.9802,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.71753418,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"14","issue":"3","first_page":"458","last_page":"63"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/riboprobes","display_name":"riboprobes","score":0.9860000014305115},{"id":"https://openalex.org/keywords/in-situ-hybridization","display_name":"in situ hybridization","score":0.8519999980926514},{"id":"https://openalex.org/keywords/multiple-cloning-site","display_name":"multiple cloning site","score":0.8149999976158142},{"id":"https://openalex.org/keywords/stromelysin-3","display_name":"stromelysin-3","score":0.9449999928474426},{"id":"https://openalex.org/keywords/lung-cancer","display_name":"lung cancer","score":0.9179999828338623},{"id":"https://openalex.org/keywords/probe-design","display_name":"probe design","score":0.4909999966621399}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7604838609695435},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6923525929450989},{"id":"https://openalex.org/C107618985","wikidata":"https://www.wikidata.org/wiki/Q358963","display_name":"Multiple cloning site","level":5,"score":0.647149920463562},{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.6216332912445068},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5381956696510315},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.500253438949585},{"id":"https://openalex.org/C168374021","wikidata":"https://www.wikidata.org/wiki/Q1924179","display_name":"Cloning vector","level":5,"score":0.4980316162109375},{"id":"https://openalex.org/C143141573","wikidata":"https://www.wikidata.org/wiki/Q7450971","display_name":"Sense (electronics)","level":2,"score":0.49687936902046204},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.48345527052879333},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3391106128692627},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3005489110946655},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.24873089790344238},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.18066895008087158},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.15344879031181335},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.1138353943824768},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.09808486700057983},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008175","descriptor_name":"Lung Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008666","descriptor_name":"Metalloendopeptidases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009363","descriptor_name":"Neoplasm Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012339","descriptor_name":"RNA, Ribosomal, 28S","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014066","descriptor_name":"Palatine Tonsil","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015336","descriptor_name":"Molecular Probe Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015347","descriptor_name":"RNA Probes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017403","descriptor_name":"In Situ Hybridization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D053507","descriptor_name":"Matrix Metalloproteinase 11","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:8457354","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8457354","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},{"id":"mag:40430544","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8457354","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2037667594","https://openalex.org/W2089655242","https://openalex.org/W4282924456","https://openalex.org/W2161559739","https://openalex.org/W1976828303","https://openalex.org/W2094287835","https://openalex.org/W2029085668","https://openalex.org/W328870440","https://openalex.org/W2314345556","https://openalex.org/W107621418"],"abstract_inverted_index":{"The":[0],"pBluescript":[1,50],"family":[2],"of":[3,16,80,118,127,132],"plasmids":[4],"and":[5,19,88,120],"phagemids":[6],"are":[7,92],"sophisticated":[8],"multi-purpose":[9],"cloning":[10,32,83],"vectors":[11],"that":[12,38,68,91],"allow":[13],"convenient":[14],"production":[15],"single-stranded":[17],"sense":[18,42,119],"anti-sense":[20,121],"RNA":[21],"probes":[22,44,122],"corresponding":[23,123],"to":[24,61,74,94,107,124,130],"DNA":[25],"sequences":[26,75],"inserted":[27],"into":[28,49],"a":[29,125],"large":[30],"multiple":[31,82],"site":[33,84],"array.":[34],"We":[35],"have":[36],"observed":[37],"in":[39,58,102,105,115],"many":[40],"applications":[41],"(control)":[43],"generated":[45],"from":[46],"genes":[47],"cloned":[48],"II":[51],"KS(-)":[52],"give":[53],"high":[54],"background":[55,110],"signals":[56],"on":[57],"situ":[59,116],"hybridization":[60,71,117],"human":[62,95,128,133],"tissue":[63],"sections.":[64],"Our":[65],"studies":[66],"indicate":[67],"this":[69],"spurious":[70],"is":[72,100],"due":[73],"contained":[76],"within":[77],"both":[78],"strands":[79],"the":[81,86],"between":[85],"SmaI":[87],"SacI":[89],"sites":[90],"similar":[93],"28S":[96],"rRNA.":[97],"This":[98],"information":[99],"useful":[101],"construct":[103],"design":[104],"order":[106],"minimize":[108],"nonspecific":[109],"problems,":[111],"as":[112],"demonstrated":[113],"by":[114],"portion":[126],"stromelysin-3":[129],"sections":[131],"lung":[134],"carcinoma.":[135]},"counts_by_year":[{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}