{"id":"https://openalex.org/W39779123","doi":null,"title":"A cellular model to mimic exhaled cigarette smokeinduced lung microvascular endothelial cell injury and death.","display_name":"A cellular model to mimic exhaled cigarette smokeinduced lung microvascular endothelial cell injury and death.","publication_year":2010,"publication_date":"2010-07-31","ids":{"openalex":"https://openalex.org/W39779123","mag":"39779123","pmid":"https://pubmed.ncbi.nlm.nih.gov/20827320","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2929948"},"language":"en","primary_location":{"id":"pmid:20827320","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20827320","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of clinical and experimental medicine","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2929948/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101583160","display_name":"Jianliang Zhang","orcid":"https://orcid.org/0000-0002-5370-8446","observed_orcids":["https://orcid.org/0000-0002-5370-8446"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jianliang Zhang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064961728","display_name":"Noah Juedes","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Noah Juedes","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067816501","display_name":"Vikram M. Narayan","orcid":"https://orcid.org/0000-0003-3731-4209","observed_orcids":["https://orcid.org/0000-0003-3731-4209"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Vikram M Narayan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087903477","display_name":"Bingfang Yue","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bingfang Yue","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029547013","display_name":"Alan L. Rockwood","orcid":"https://orcid.org/0000-0002-3792-1204","observed_orcids":["https://orcid.org/0000-0002-3792-1204"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Alan L Rockwood","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031205090","display_name":"Nadia L. Palma","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nadia L Palma","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5111808777","display_name":"Jawaharlal M. Patel","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jawaharlal M Patel","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.3173,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.66292085,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":92,"max":97},"biblio":{"volume":"3","issue":"3","first_page":"223","last_page":"32"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10060","display_name":"Smoking Behavior and Cessation","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10060","display_name":"Smoking Behavior and Cessation","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10143","display_name":"Chronic Obstructive Pulmonary Disease (COPD) Research","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10155","display_name":"Nitric Oxide and Endothelin Effects","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/passive-smoking","display_name":"passive smoking","score":0.9369999766349792},{"id":"https://openalex.org/keywords/lung-microvascular-endothelial-cells","display_name":"lung microvascular endothelial cells","score":0.9020000100135803},{"id":"https://openalex.org/keywords/endothelial-injury","display_name":"endothelial injury","score":0.8799999952316284},{"id":"https://openalex.org/keywords/cell-death","display_name":"cell death","score":0.8399999737739563},{"id":"https://openalex.org/keywords/nicotine","display_name":"nicotine","score":0.6579999923706055},{"id":"https://openalex.org/keywords/cotinine","display_name":"cotinine","score":0.9990000128746033},{"id":"https://openalex.org/keywords/alveolar-wall-destruction","display_name":"alveolar wall destruction","score":0.7210000157356262},{"id":"https://openalex.org/keywords/in-vitro-exposure-model","display_name":"in vitro exposure model","score":0.6299999952316284},{"id":"https://openalex.org/keywords/cell-viability","display_name":"cell viability","score":0.5389999747276306},{"id":"https://openalex.org/keywords/cell-detachment","display_name":"cell detachment","score":0.6520000100135803}],"concepts":[{"id":"https://openalex.org/C129990765","wikidata":"https://www.wikidata.org/wiki/Q7508482","display_name":"Sidestream smoke","level":3,"score":0.8285049796104431},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.6715719699859619},{"id":"https://openalex.org/C2777714996","wikidata":"https://www.wikidata.org/wiki/Q7886","display_name":"Lung","level":2,"score":0.6407280564308167},{"id":"https://openalex.org/C2910307670","wikidata":"https://www.wikidata.org/wiki/Q2133097","display_name":"Smoke Inhalation Injury","level":4,"score":0.629197359085083},{"id":"https://openalex.org/C2779547902","wikidata":"https://www.wikidata.org/wiki/Q28086552","display_name":"Nicotine","level":2,"score":0.6195652484893799},{"id":"https://openalex.org/C2778355491","wikidata":"https://www.wikidata.org/wiki/Q2386268","display_name":"Tobacco smoke","level":2,"score":0.5718156099319458},{"id":"https://openalex.org/C2776319443","wikidata":"https://www.wikidata.org/wiki/Q421177","display_name":"Cotinine","level":3,"score":0.5716661810874939},{"id":"https://openalex.org/C58874564","wikidata":"https://www.wikidata.org/wiki/Q130768","display_name":"Smoke","level":2,"score":0.5272727608680725},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.4609319567680359},{"id":"https://openalex.org/C2777390754","wikidata":"https://www.wikidata.org/wiki/Q2133097","display_name":"Smoke inhalation","level":3,"score":0.4226875305175781},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.40711769461631775},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.3873634934425354},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3575478196144104},{"id":"https://openalex.org/C2993351451","wikidata":"https://www.wikidata.org/wiki/Q2386268","display_name":"Cigarette smoke","level":2,"score":0.3025677800178528},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.19779938459396362},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.18405365943908691},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmid:20827320","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20827320","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of clinical and experimental medicine","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2929948","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2929948/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Int J Clin Exp Med","raw_type":"Text"},{"id":"mag:39779123","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20827320","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2929948","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2929948/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Int J Clin Exp Med","raw_type":"Text"},"sustainable_development_goals":[{"score":0.9763000011444092,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4594926734","display_name":"CALRETICULIN REGULATION OF LUNG ENDOTHELIAL CELL NOS","funder_award_id":"R01HL068666","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G8112425330","display_name":"Novel Peptide for Dynamic Regulation of Lung Endothelium NOS/cGMP Functions","funder_award_id":"R01HL085133","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W153904168","https://openalex.org/W1497098097","https://openalex.org/W1585287391","https://openalex.org/W1870141733","https://openalex.org/W1966061020","https://openalex.org/W1989314580","https://openalex.org/W1990840234","https://openalex.org/W2001734241","https://openalex.org/W2001890949","https://openalex.org/W2003344121","https://openalex.org/W2004803270","https://openalex.org/W2013217130","https://openalex.org/W2030892279","https://openalex.org/W2032979359","https://openalex.org/W2033845596","https://openalex.org/W2038979163","https://openalex.org/W2049285262","https://openalex.org/W2081920314","https://openalex.org/W2106299783","https://openalex.org/W2110615895","https://openalex.org/W2111828045","https://openalex.org/W2116438644","https://openalex.org/W2124631075","https://openalex.org/W2125308937","https://openalex.org/W2144970165","https://openalex.org/W2202540163","https://openalex.org/W2395700795","https://openalex.org/W2406610221","https://openalex.org/W2408178740"],"related_works":["https://openalex.org/W2169358061","https://openalex.org/W2302324388","https://openalex.org/W2781887955","https://openalex.org/W2080842362","https://openalex.org/W153904168","https://openalex.org/W1982021097","https://openalex.org/W2401064442","https://openalex.org/W2883307761","https://openalex.org/W6969026809","https://openalex.org/W1877733676"],"abstract_inverted_index":{"Tobacco":[0,142],"smoke":[1,28,39,61,68,81,143,242],"exhaled":[2,38,60,79],"from":[3,71,191],"smokers":[4],"is":[5,35],"a":[6,72,127,208],"key":[7],"component":[8],"of":[9,59,84,98,130,177,218,238,257],"secondhand":[10],"smoke,":[11],"contributing":[12],"to":[13,31,49,55,77,86,89,138,167,179,182,187,244,254],"lung":[14,21,32,41,63,150,160,203,260],"alveolar":[15],"wall":[16],"destruction":[17],"seen":[18],"in":[19,52,92,121],"chronic":[20],"diseases.":[22,261],"Although":[23],"mainstream":[24,240],"and":[25,169,174,230,248],"sidestream":[26],"tobacco":[27,80],"are":[29,116],"cyto-toxic":[30],"cells,":[33],"it":[34,153],"unclear":[36],"whether":[37],"induces":[40,227],"cell":[42,172,204,212,221,228,249],"injury":[43,173],"or":[44],"even":[45],"death.":[46,175],"We":[47],"sought":[48],"establish":[50],"an":[51,103],"vitro":[53],"model":[54],"examine":[56],"the":[57,99,122,145,155,192,195,215,255],"effects":[58],"on":[62],"cells.":[64],"Phosphate-buffered":[65],"saline-conditioned":[66],"cigarette":[67,241],"(CCS)":[69],"derived":[70],"blow-by":[73],"system":[74],"was":[75],"used":[76,137],"mimic":[78],"exposure.":[82,141],"Exposure":[83,176],"medium":[85,101],"CCS":[87,140,168,180,200,206,219,225],"leads":[88,186,243],"dose-dependent":[90],"increases":[91,211],"nicotine/cotinine":[93,246],"levels.":[94],"Scanning":[95],"spectrophotometric":[96],"analysis":[97],"CCS-exposed":[100],"reveals":[102],"absorption":[104],"peak":[105],"at":[106,113,132,149,207],"290":[107,114,133],"nm":[108,115,134],"wavelength.":[109],"The":[110],"OD":[111,131],"values":[112],"correlated":[117],"with":[118],"nicotine":[119],"levels":[120,247],"exposed":[123,166],"medium,":[124],"indicating":[125],"that":[126,199,236],"simple":[128],"measurement":[129],"can":[135,201],"be":[136],"monitor":[139],"contacts":[144],"microvascular":[146,161],"endothelium":[147],"located":[148],"alveoli,":[151],"before":[152],"enters":[154],"blood":[156],"stream.":[157],"Hence,":[158],"human":[159],"endothelial":[162],"cells":[163,193],"(hMVEC)":[164],"were":[165],"assessed":[170],"for":[171],"hMVEC":[178],"equivalent":[181],"burning":[183],"12-16":[184],"cigarettes":[185],"increased":[188,245],"LDH":[189],"release":[190],"into":[194],"medium.":[196],"This":[197],"suggests":[198],"induce":[202],"injury.":[205],"low":[209],"level":[210,217],"growth,":[213],"whereas":[214],"high":[216],"decreases":[220],"viability.":[222],"In":[223],"addition,":[224],"exposure":[226,237],"detachment":[229],"morphological":[231],"changes.":[232],"Our":[233],"results":[234],"demonstrate":[235],"buffer-conditioned":[239],"injury/death,":[250],"which":[251],"may":[252],"contribute":[253],"pathophysiology":[256],"passive":[258],"smoking-associated":[259]},"counts_by_year":[{"year":2021,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":3}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}