{"id":"https://openalex.org/W36507799","doi":null,"title":"A novel strategy for myocardial protection using in vivo transfection of cis element 'decoy' against NFkappaB binding site: evidence for a role of NFkappaB in ischemia-reperfusion injury.","display_name":"A novel strategy for myocardial protection using in vivo transfection of cis element 'decoy' against NFkappaB binding site: evidence for a role of NFkappaB in ischemia-reperfusion injury.","publication_year":1997,"publication_date":"1997-11-04","ids":{"openalex":"https://openalex.org/W36507799","mag":"36507799","pmid":"https://pubmed.ncbi.nlm.nih.gov/9386111"},"language":"en","primary_location":{"id":"pmid:9386111","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9386111","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Circulation","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045683397","display_name":"Yoshiki Sawa","orcid":"https://orcid.org/0000-0001-6132-9684","observed_orcids":["https://orcid.org/0000-0001-6132-9684"]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Y Sawa","raw_affiliation_strings":["First Department of Surgery, Institute for Cellular and Molecular Biology, and Fujisawa Pharmaceutical Co, Ltd, Osaka University Medical School, Suita, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"First Department of Surgery, Institute for Cellular and Molecular Biology, and Fujisawa Pharmaceutical Co, Ltd, Osaka University Medical School, Suita, Japan","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025005882","display_name":"Ryuichi Morishita","orcid":"https://orcid.org/0000-0002-8855-0730","observed_orcids":["https://orcid.org/0000-0002-8855-0730"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R Morishita","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062288838","display_name":"Ken Suzuki","orcid":"https://orcid.org/0000-0002-8970-1553","observed_orcids":["https://orcid.org/0000-0002-8970-1553"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K Suzuki","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111601041","display_name":"K Kagisaki","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K Kagisaki","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102026777","display_name":"Yasufumi Kaneda","orcid":"https://orcid.org/0009-0009-6106-5434","observed_orcids":["https://orcid.org/0009-0009-6106-5434"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Y Kaneda","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017230904","display_name":"Kiyoshi Maeda","orcid":"https://orcid.org/0000-0002-5138-4767","observed_orcids":["https://orcid.org/0000-0002-5138-4767"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K Maeda","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109959883","display_name":"Keishi Kadoba","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K Kadoba","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5058261979","display_name":"Hikaru Matsuda","orcid":"https://orcid.org/0000-0002-7805-7192","observed_orcids":["https://orcid.org/0000-0002-7805-7192","https://orcid.org/0009-0003-1597-9098"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Matsuda","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.0831,"has_fulltext":false,"cited_by_count":91,"citation_normalized_percentile":{"value":0.94139965,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":96},"biblio":{"volume":"96","issue":"9 Suppl","first_page":"II","last_page":"280"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11057","display_name":"Cardiac Ischemia and Reperfusion","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11057","display_name":"Cardiac Ischemia and Reperfusion","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11591","display_name":"NF-\u03baB Signaling Pathways","score":0.00279999990016222,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11055","display_name":"Cardiac and Coronary Surgery Techniques","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cardioprotection","display_name":"cardioprotection","score":0.746999979019165},{"id":"https://openalex.org/keywords/nf-kappab","display_name":"NF-\u03baB","score":0.8970000147819519},{"id":"https://openalex.org/keywords/ischemia-reperfusion-injury","display_name":"ischemia-reperfusion injury","score":0.8920000195503235},{"id":"https://openalex.org/keywords/in-vivo-gene-transfection","display_name":"in vivo gene transfection","score":0.8550000190734863},{"id":"https://openalex.org/keywords/hvj-liposome","display_name":"HVJ-liposome","score":0.7459999918937683},{"id":"https://openalex.org/keywords/cardioplegic-arrest","display_name":"cardioplegic arrest","score":0.8809999823570251},{"id":"https://openalex.org/keywords/neutrophil-adhesion","display_name":"neutrophil adhesion","score":0.9129999876022339},{"id":"https://openalex.org/keywords/interleukin-8","display_name":"interleukin-8","score":0.9940000176429749},{"id":"https://openalex.org/keywords/coronary-blood-flow","display_name":"coronary blood flow","score":0.4309999942779541},{"id":"https://openalex.org/keywords/left-ventricular-function","display_name":"left ventricular function","score":0.6729999780654907}],"concepts":[{"id":"https://openalex.org/C2779179475","wikidata":"https://www.wikidata.org/wiki/Q3545649","display_name":"Decoy","level":3,"score":0.8176175951957703},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.7629262208938599},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.7465978860855103},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.5652835369110107},{"id":"https://openalex.org/C2780114680","wikidata":"https://www.wikidata.org/wiki/Q1413991","display_name":"Reperfusion injury","level":3,"score":0.48869588971138},{"id":"https://openalex.org/C2778690821","wikidata":"https://www.wikidata.org/wiki/Q212354","display_name":"Cytokine","level":2,"score":0.4490877687931061},{"id":"https://openalex.org/C541997718","wikidata":"https://www.wikidata.org/wiki/Q188151","display_name":"Ischemia","level":2,"score":0.43814817070961},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.37479740381240845},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.34858906269073486},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.3241952955722809},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.2687014937400818},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.21477213501930237},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.16258728504180908},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.14348021149635315},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13551628589630127},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002448","descriptor_name":"Cell Adhesion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009504","descriptor_name":"Neutrophils","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015316","descriptor_name":"Genetic Therapy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015428","descriptor_name":"Myocardial Reperfusion Injury","qualifier_ui":"Q000209","qualifier_name":"etiology","is_major_topic":true},{"descriptor_ui":"D016209","descriptor_name":"Interleukin-8","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D016328","descriptor_name":"NF-kappa B","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016328","descriptor_name":"NF-kappa B","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D017207","descriptor_name":"Rats, Sprague-Dawley","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:9386111","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9386111","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Circulation","raw_type":"Journal Article"},{"id":"mag:36507799","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9386111","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.979200005531311,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1996343873","https://openalex.org/W2027835440","https://openalex.org/W1979360986","https://openalex.org/W2031344267","https://openalex.org/W2376550798","https://openalex.org/W2318876874","https://openalex.org/W2183290946","https://openalex.org/W2056668196","https://openalex.org/W2086309499","https://openalex.org/W2006326400"],"abstract_inverted_index":{"BACKGROUND:":[0],"NFkappaB,":[1],"an":[2,50],"important":[3],"transcriptional":[4,38],"factor,":[5],"has":[6],"been":[7],"reported":[8],"to":[9,48,58,185,210,291],"play":[10],"a":[11,203,217,269,293],"significant":[12,245,270],"role":[13,271],"in":[14,33,120,247,252,272,278],"the":[15,26,34,60,112,121,126,132,140,152,180,229,255],"coordinated":[16],"transcription":[17],"of":[18,62,102,105,123,128,134,150,159,161,207,221,257,282],"cytokine":[19],"and":[20,52,93,114,172,192,216,260,295],"adhesion":[21],"molecule":[22],"genes.":[23],"Therefore,":[24],"blocking":[25,37],"NFkappaB":[27,67,87,241,267,287],"may":[28],"attenuate":[29],"ischemia":[30,186],"reperfusion":[31,193],"injury":[32,251],"myocardium.":[35],"For":[36],"factors,":[39],"gene":[40,129,280],"therapy,":[41],"such":[42],"as":[43,137],"cis":[44,63,83,237,283],"element":[45,64,84,238,284],"\"decoy,\"":[46],"appears":[47,290],"be":[49,292],"innovative":[51],"useful":[53],"therapy.":[54],"This":[55,264,275],"study":[56],"aimed":[57],"prove":[59],"efficacy":[61,127],"decoy":[65,85,95,239,285],"against":[66,86,240,249,286],"binding":[68,242,288],"site":[69,89,243],"for":[70,298],"myocardial":[71,299],"protection.":[72,300],"METHODS":[73],"AND":[74],"RESULTS:":[75],"Rat":[76],"hearts":[77,182,234],"were":[78],"transfected":[79,143,235],"with":[80,139,144,236,254],"fluorescence":[81],"isothiocyanate-labeled":[82],"(NF)-binding":[88],"(NF":[90,166],"group,":[91],"n=6)":[92],"scrambled":[94],"(SD)":[96],"group":[97,142,154,201],"(n=6)":[98],"by":[99],"coronary":[100,173],"infusion":[101],"hemagglutinating":[103],"virus":[104],"Japan":[106],"(HVJ)-liposome":[107],"during":[108],"cardioplegic":[109],"arrest.":[110],"Both":[111],"NF":[113,153,200],"SD":[115,230],"groups":[116],"showed":[117,155,202,244],"marked":[118],"FITC-staining":[119],"nuclei":[122,133],"myocytes,":[124],"demonstrating":[125],"transfer":[130],"into":[131],"cardiac":[135],"myocytes":[136],"compared":[138],"control":[141,181],"empty":[145],"liposomes.":[146],"After":[147],"3":[148],"days":[149],"transfection,":[151],"significantly":[156,204],"higher":[157],"percentages":[158],"recovery":[160],"left":[162],"ventricular":[163],"developed":[164],"pressure":[165],"versus":[167,170,176,214,224],"SD,":[168],"87+/-11":[169],"54+/-12%)":[171],"flow":[174],"(97+/-16":[175],"61+/-15%)":[177],"than":[178,227],"did":[179,228],"when":[183],"exposed":[184],"(30":[187,194],"minutes,":[188,195],"37":[189,196],"degrees":[190,197],"C)":[191],"C).":[198],"The":[199,233],"lower":[205,218],"percentage":[206],"neutrophil":[208,258],"adherence":[209,259],"endothelial":[211],"cells":[212],"(38+/-6":[213],"81+/-3%)":[215],"tissue":[219,261],"level":[220],"interleukin-8":[222],"(109+/-48":[223],"210+/-55":[225],"ng/mg)":[226],"group.":[231],"CONCLUSION:":[232],"improvement":[246],"tolerance":[248],"ischemia-reperfusion":[250,273],"association":[253],"inhibition":[256],"IL-8":[262],"production.":[263],"suggests":[265],"that":[266],"plays":[268],"injury.":[274],"method,":[276],"using":[277],"vivo":[279],"transfection":[281],"site,":[289],"novel":[294],"future":[296],"strategy":[297]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}