{"id":"https://openalex.org/W36144294","doi":null,"title":"Computational Approaches for Structure Based Drug Design and Protein Structure-Function Prediction","display_name":"Computational Approaches for Structure Based Drug Design and Protein Structure-Function Prediction","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W36144294","mag":"36144294"},"language":"en","primary_location":{"id":"pmh:oai:scholarcommons.usf.edu:etd-5798","doi":null,"is_oa":true,"landing_page_url":"https://scholarcommons.usf.edu/etd/4601","pdf_url":"https://scholarcommons.usf.edu/cgi/viewcontent.cgi?article=5798&context=etd","source":{"id":"https://openalex.org/S4377196272","display_name":"Digital Commons - University of South Florida (University of South Florida)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2613432","host_organization_name":"University of South Florida","host_organization_lineage":["https://openalex.org/I2613432"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://scholarcommons.usf.edu/cgi/viewcontent.cgi?article=5798&context=etd","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051303435","display_name":"Sai Lakshmana Vankayala","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Sai Lakshmana Vankayala","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5051303435"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.0015855,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":92},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10211","display_name":"Computational Drug Discovery Methods","score":0.9404000043869019,"subfield":{"id":"https://openalex.org/subfields/3002","display_name":"Drug Discovery"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10211","display_name":"Computational Drug Discovery Methods","score":0.9404000043869019,"subfield":{"id":"https://openalex.org/subfields/3002","display_name":"Drug Discovery"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.02500000037252903,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.003700000001117587,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/structure-based-drug-design","display_name":"structure-based drug design","score":0.9660000205039978},{"id":"https://openalex.org/keywords/induced-fit-docking","display_name":"induced fit docking","score":0.8410000205039978},{"id":"https://openalex.org/keywords/flexible-docking","display_name":"flexible docking","score":0.8970000147819519},{"id":"https://openalex.org/keywords/virtual-screening","display_name":"virtual screening","score":0.5529999732971191},{"id":"https://openalex.org/keywords/sickle-cell-disease","display_name":"sickle cell disease","score":0.8659999966621399},{"id":"https://openalex.org/keywords/hydroxyurea","display_name":"hydroxyurea","score":0.8960000276565552},{"id":"https://openalex.org/keywords/nitric-oxide","display_name":"nitric oxide","score":0.8679999709129333},{"id":"https://openalex.org/keywords/catalase","display_name":"catalase","score":0.7689999938011169},{"id":"https://openalex.org/keywords/peroxidase","display_name":"peroxidase","score":0.6399999856948853},{"id":"https://openalex.org/keywords/substrate-binding-mode","display_name":"substrate binding mode","score":0.7089999914169312},{"id":"https://openalex.org/keywords/o6-methylguanine-dna-methyltransferase","display_name":"O6-methylguanine-DNA methyltransferase","score":0.9639999866485596},{"id":"https://openalex.org/keywords/monte-carlo-conformational-search","display_name":"Monte Carlo conformational search","score":0.8389999866485596}],"concepts":[{"id":"https://openalex.org/C18051474","wikidata":"https://www.wikidata.org/wiki/Q899656","display_name":"Protein structure prediction","level":3,"score":0.6984602212905884},{"id":"https://openalex.org/C2984217123","wikidata":"https://www.wikidata.org/wiki/Q25099949","display_name":"Structure function","level":2,"score":0.55677330493927},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.5185028314590454},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5104457139968872},{"id":"https://openalex.org/C2780035454","wikidata":"https://www.wikidata.org/wiki/Q8386","display_name":"Drug","level":2,"score":0.41119930148124695},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.33684301376342773},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.32545775175094604},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.1878574788570404},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.14923980832099915},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.13424387574195862},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C109214941","wikidata":"https://www.wikidata.org/wiki/Q18334","display_name":"Particle physics","level":1,"score":0.0},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:scholarcommons.usf.edu:etd-5798","doi":null,"is_oa":true,"landing_page_url":"https://scholarcommons.usf.edu/etd/4601","pdf_url":"https://scholarcommons.usf.edu/cgi/viewcontent.cgi?article=5798&context=etd","source":{"id":"https://openalex.org/S4377196272","display_name":"Digital Commons - University of South Florida (University of South Florida)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2613432","host_organization_name":"University of South Florida","host_organization_lineage":["https://openalex.org/I2613432"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"},{"id":"pmh:oai:digitalcommons.usf.edu:etd-5798","doi":null,"is_oa":false,"landing_page_url":"https://digitalcommons.usf.edu/etd/4601","pdf_url":null,"source":{"id":"https://openalex.org/S4377196272","display_name":"Digital Commons - University of South Florida (University of South Florida)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2613432","host_organization_name":"University of South Florida","host_organization_lineage":["https://openalex.org/I2613432"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"USF Tampa Graduate Theses and Dissertations","raw_type":"dissertation"},{"id":"mag:36144294","doi":null,"is_oa":false,"landing_page_url":"https://digitalcommons.usf.edu/cgi/viewcontent.cgi?article=5798&context=etd","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:scholarcommons.usf.edu:etd-5798","doi":null,"is_oa":true,"landing_page_url":"https://scholarcommons.usf.edu/etd/4601","pdf_url":"https://scholarcommons.usf.edu/cgi/viewcontent.cgi?article=5798&context=etd","source":{"id":"https://openalex.org/S4377196272","display_name":"Digital Commons - University of South Florida (University of South Florida)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2613432","host_organization_name":"University of South Florida","host_organization_lineage":["https://openalex.org/I2613432"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.6240000128746033,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320310847","display_name":"University of South Florida","ror":"https://ror.org/032db5x82"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W36144294.pdf","grobid_xml":"https://content.openalex.org/works/W36144294.grobid-xml"},"referenced_works_count":165,"referenced_works":["https://openalex.org/W15596153","https://openalex.org/W107960928","https://openalex.org/W144524225","https://openalex.org/W1484078749","https://openalex.org/W1529327704","https://openalex.org/W1537821775","https://openalex.org/W1539899672","https://openalex.org/W1547075580","https://openalex.org/W1582109070","https://openalex.org/W1589413892","https://openalex.org/W1605578858","https://openalex.org/W1806652950","https://openalex.org/W1892290338","https://openalex.org/W1901175601","https://openalex.org/W1964692086","https://openalex.org/W1965706775","https://openalex.org/W1966142937","https://openalex.org/W1969193253","https://openalex.org/W1971618381","https://openalex.org/W1972168561","https://openalex.org/W1972297061","https://openalex.org/W1973462822","https://openalex.org/W1973974137","https://openalex.org/W1974422922","https://openalex.org/W1974649841","https://openalex.org/W1974937987","https://openalex.org/W1974977468","https://openalex.org/W1975691556","https://openalex.org/W1977508606","https://openalex.org/W1977678718","https://openalex.org/W1978949176","https://openalex.org/W1978977657","https://openalex.org/W1979648690","https://openalex.org/W1980949045","https://openalex.org/W1981064726","https://openalex.org/W1985052577","https://openalex.org/W1986240377","https://openalex.org/W1989122447","https://openalex.org/W1991483463","https://openalex.org/W1992709071","https://openalex.org/W1993997982","https://openalex.org/W1994067196","https://openalex.org/W1999549596","https://openalex.org/W1999665302","https://openalex.org/W2004967944","https://openalex.org/W2007560426","https://openalex.org/W2010439031","https://openalex.org/W2012969029","https://openalex.org/W2014372020","https://openalex.org/W2017985800","https://openalex.org/W2019701333","https://openalex.org/W2023083727","https://openalex.org/W2023940675","https://openalex.org/W2024469135","https://openalex.org/W2025151994","https://openalex.org/W2025515800","https://openalex.org/W2026141986","https://openalex.org/W2026609683","https://openalex.org/W2027023138","https://openalex.org/W2028843689","https://openalex.org/W2029087609","https://openalex.org/W2030423316","https://openalex.org/W2031259080","https://openalex.org/W2034266737","https://openalex.org/W2035460800","https://openalex.org/W2035494124","https://openalex.org/W2035698634","https://openalex.org/W2037480267","https://openalex.org/W2037694981","https://openalex.org/W2038542598","https://openalex.org/W2041995873","https://openalex.org/W2042004447","https://openalex.org/W2042021080","https://openalex.org/W2043116111","https://openalex.org/W2046214500","https://openalex.org/W2047279058","https://openalex.org/W2048363942","https://openalex.org/W2048605424","https://openalex.org/W2051163038","https://openalex.org/W2051381895","https://openalex.org/W2052030759","https://openalex.org/W2056070442","https://openalex.org/W2056179306","https://openalex.org/W2056937556","https://openalex.org/W2057153853","https://openalex.org/W2057846043","https://openalex.org/W2059029931","https://openalex.org/W2061079400","https://openalex.org/W2061750520","https://openalex.org/W2062991061","https://openalex.org/W2065061109","https://openalex.org/W2065355613","https://openalex.org/W2066962978","https://openalex.org/W2068463090","https://openalex.org/W2070753604","https://openalex.org/W2073823496","https://openalex.org/W2074135025","https://openalex.org/W2078992248","https://openalex.org/W2079075641","https://openalex.org/W2083485969","https://openalex.org/W2086405025","https://openalex.org/W2087293823","https://openalex.org/W2088102370","https://openalex.org/W2089078246","https://openalex.org/W2090338886","https://openalex.org/W2091450709","https://openalex.org/W2092175995","https://openalex.org/W2092434707","https://openalex.org/W2094704437","https://openalex.org/W2096920054","https://openalex.org/W2099532120","https://openalex.org/W2101043007","https://openalex.org/W2102377211","https://openalex.org/W2102384571","https://openalex.org/W2103135489","https://openalex.org/W2103300124","https://openalex.org/W2103591844","https://openalex.org/W2103994532","https://openalex.org/W2105668062","https://openalex.org/W2106383417","https://openalex.org/W2112086982","https://openalex.org/W2114819818","https://openalex.org/W2121087546","https://openalex.org/W2121560055","https://openalex.org/W2124126888","https://openalex.org/W2127290087","https://openalex.org/W2128332459","https://openalex.org/W2129153475","https://openalex.org/W2130479394","https://openalex.org/W2132262459","https://openalex.org/W2133641393","https://openalex.org/W2135817448","https://openalex.org/W2137015675","https://openalex.org/W2137604324","https://openalex.org/W2138545604","https://openalex.org/W2138770756","https://openalex.org/W2139974478","https://openalex.org/W2147993766","https://openalex.org/W2150018605","https://openalex.org/W2152630148","https://openalex.org/W2156053468","https://openalex.org/W2158167632","https://openalex.org/W2158360182","https://openalex.org/W2158534713","https://openalex.org/W2159006106","https://openalex.org/W2160308620","https://openalex.org/W2162967120","https://openalex.org/W2164626973","https://openalex.org/W2165273498","https://openalex.org/W2165770167","https://openalex.org/W2165838079","https://openalex.org/W2166382251","https://openalex.org/W2167575821","https://openalex.org/W2167895705","https://openalex.org/W2169984716","https://openalex.org/W2170932866","https://openalex.org/W2206904580","https://openalex.org/W2280080732","https://openalex.org/W2313541759","https://openalex.org/W2321974263","https://openalex.org/W2324788201","https://openalex.org/W2412638920","https://openalex.org/W2952125901","https://openalex.org/W3146570008","https://openalex.org/W4249969224"],"related_works":["https://openalex.org/W2099532120","https://openalex.org/W3024760158","https://openalex.org/W4313457522","https://openalex.org/W2804784254","https://openalex.org/W7039820943","https://openalex.org/W4211071040","https://openalex.org/W4408756916","https://openalex.org/W2120406481","https://openalex.org/W2379003809","https://openalex.org/W2171405825"],"abstract_inverted_index":{"This":[0,54],"dissertation":[1],"thesis":[2],"consists":[3],"of":[4,7,32,35,41,80,85,97,130,148,164,172,182,209,225,248,254,291,294,306,318,348,381,395,407,421,466,489],"a":[5,252,292,322,344,352,413,440,453],"series":[6,293],"chapters":[8],"that":[9,70,269,327,365,449],"are":[10,92,311],"interwoven":[11],"by":[12],"solving":[13],"interesting":[14],"biological":[15],"problems,":[16],"employing":[17],"various":[18],"computational":[19,42],"methodologies.":[20],"These":[21],"techniques":[22],"provide":[23],"meaningful":[24],"physical":[25],"insights":[26,330],"to":[27,106,110,153,222,283,297,470],"promote":[28],"the":[29,39,58,72,77,95,112,128,143,161,169,176,179,206,223,244,274,285,302,307,396,422,450,460,467,477],"scientific":[30],"fields":[31],"interest.":[33],"Focus":[34],"chapter":[36,55,339],"1":[37],"concerns,":[38],"importance":[40,96],"tools":[43,279],"like":[44,61],"docking":[45,68,73,100,435],"studies":[46,69,91,101,485],"in":[47,83,104,175,195,212,338,487],"advancing":[48],"structure":[49,347],"based":[50,231],"drug":[51],"design":[52],"processes.":[53],"also":[56],"addresses":[57],"prime":[59],"concerns":[60],"scoring":[62],"functions,":[63],"sampling":[64],"algorithms":[65],"and":[66,89,117,133,192,236,301,315,321,371,393,399],"flexible":[67,81],"hamper":[71],"successes.":[74],"Information":[75],"about":[76],"different":[78],"kinds":[79],"dockings":[82],"terms":[84],"accuracy,":[86],"time":[87],"limitations":[88],"success":[90],"presented.":[93],"Later":[94],"Induced":[98],"fit":[99,262],"was":[102,444],"explained":[103],"comparison":[105],"traditional":[107],"MD":[108],"simulations":[109],"predict":[111],"absolute":[113],"binding":[114,289,335],"modes.\\nChapter":[115],"2":[116],"3":[118],"focuses":[119],"on":[120,136],"understanding,":[121],"how":[122],"sickle":[123,137,149,155],"cell":[124,138,150,156],"disease":[125,151],"progresses":[126],"through":[127],"production":[129,198],"sickled":[131],"hemoglobin":[132],"its":[134],"effects":[135],"patients.":[139],"And":[140],"how,":[141],"hydroxyurea,":[142],"only":[144],"FDA":[145],"approved":[146],"treatment":[147],"acts":[152],"subside":[154],"effects.":[157],"It":[158,362],"is":[159,166,185,325,363],"believed":[160],"primary":[162],"mechanism":[163,184,324],"action":[165],"associated":[167],"with":[168,190,205,228,266,313,410,457,476,493],"pharmacological":[170],"elevation":[171],"nitric":[173],"oxide":[174],"blood,":[177],"however,":[178],"exact":[180],"details":[181,247],"this":[183,201,249,480],"still":[186],"unclear.":[187],"HU":[188,215,226],"interacts":[189],"oxy":[191],"deoxyHb":[193],"resulting":[194],"slow":[196],"NO":[197,210],"rates.":[199],"However,":[200],"did":[202],"not":[203],"correlate":[204],"observed":[207],"increase":[208],"concentrations":[211],"patients":[213],"undergoing":[214],"therapy.":[216],"The":[217,391,419,446],"discrepancy":[218],"can":[219],"be":[220],"attributed":[221],"interaction":[224,271,465],"competing":[227],"other":[229],"heme":[230],"enzymes":[232],"such":[233],"as":[234,388],"catalase":[235,300],"peroxidases.":[237],"In":[238],"these":[239,278,417],"two":[240],"chapters,":[241],"we":[242,280,341],"investigate":[243],"atomic":[245,275],"level":[246],"process":[250],"using":[251,356],"combination":[253],"flexible-ligand":[255],"/":[256],"flexible-receptor":[257],"virtual":[258],"screening":[259,484],"(i.e.":[260],"induced":[261],"docking,":[263],"IFD)":[264],"coupled":[265],"energetic":[267],"analysis":[268,443],"decomposes":[270],"energies":[272],"at":[273],"level.":[276],"Using":[277,479],"were":[281,384],"able":[282],"elucidate":[284],"previously":[286],"unknown":[287],"substrate":[288,334],"modes":[290],"hydroxyurea":[295],"analogs":[296],"human":[298],"hemoglobin,":[299],"concomitant":[303],"structural":[304],"changes":[305],"enzymes.":[308],"Our":[309],"results":[310],"consistent":[312],"kinetic":[314],"EPR":[316],"measurements":[317],"hydroxyurea-hemoglobin":[319],"reactions":[320],"full":[323],"proposed":[326],"offers":[328],"new":[329],"into":[331],"possibly":[332],"improving":[333],"and/or":[336],"reactivity.\\nFinally":[337],"4,":[340],"have":[342],"developed":[343],"3D":[345],"bioactive":[346],"O6-alkylguanine-DNA":[349],"alkyltransferase":[350,411],"(AGT),":[351],"DNA":[353,368],"repair":[354],"protein":[355],"Monte":[357],"Carlo":[358],"conformational":[359],"search":[360],"process.":[361],"known":[364],"AGT":[366],"prevents":[367],"damage,":[369],"mutations":[370],"apoptosis":[372],"arising":[373],"from":[374,432,496],"alkylated":[375],"guanines.":[376],"Various":[377],"Benzyl":[378],"guanine":[379],"analouges":[380],"O6-":[382],"methylguanine":[383],"tested":[385],"for":[386,416,482],"activity":[387],"potential":[389],"inhibitors.":[390],"nature":[392],"position":[394],"substitutions":[397],"methyl":[398],"aminomethyl":[400],"profoundly":[401],"affected":[402],"their":[403,408],"activity.":[404],"Molecular":[405],"modeling":[406],"interactions":[409],"provided":[412],"molecular":[414],"explanation":[415],"results.":[418],"square":[420],"correlation":[423],"coef\ufb01cient":[424],"(R2":[425],")":[426],"obtained":[427],"between":[428],"E-model":[429],"scores":[430],"(obtained":[431],"GLIDE":[433],"XP/QPLD":[434],"calculations)":[436],"vs":[437],"log(ED)values":[438],"via":[439],"linear":[441],"regression":[442],"0.96.":[445],"models":[447],"indicate":[448],"ortho-substitution":[451],"causes":[452],"steric":[454],"clash":[455],"interfering":[456],"binding,":[458],"whereas":[459],"meta-aminomethyl":[461],"substitution":[462],"allows":[463],"an":[464,472],"amino":[468],"group":[469],"generate":[471],"additional":[473],"hydrogen":[474],"bond":[475],"protein.":[478],"model":[481],"virtually":[483],"resulted":[486],"identification":[488],"seven":[490],"lead":[491],"compounds":[492],"novel":[494],"scaffolds":[495],"National":[497],"Cancer":[498],"Institute":[499],"Diversity":[500],"Set2.":[501]},"counts_by_year":[{"year":2021,"cited_by_count":1}],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2016-06-24T00:00:00"}
