{"id":"https://openalex.org/W35777511","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.439.6","title":"RNA interference techniques as an approach to elucidating ranavirus gene function.","display_name":"RNA interference techniques as an approach to elucidating ranavirus gene function.","publication_year":2009,"publication_date":"2009-04-01","ids":{"openalex":"https://openalex.org/W35777511","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.439.6","mag":"35777511"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.23.1_supplement.439.6","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.439.6","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.23.1_supplement.439.6","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5012597016","display_name":"Robert Cressten Sample","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Robert Cressten Sample","raw_affiliation_strings":["Microbiology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microbiology","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062719600","display_name":"Dexter S. Whitley","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dexter Whitley","raw_affiliation_strings":["Microbiology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microbiology","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046775881","display_name":"Jeffrey R. Henegar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jeffrey Henegar","raw_affiliation_strings":["Pathology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pathology","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066299587","display_name":"Donna C. Sullivan","orcid":"https://orcid.org/0000-0001-5413-4176","observed_orcids":["https://orcid.org/0000-0001-5413-4176"]},"institutions":[{"id":"https://openalex.org/I29606459","display_name":"University of Mississippi Medical Center","ror":"https://ror.org/044pcn091","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I29606459","https://openalex.org/I4210141039"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Donna C Sullivan","raw_affiliation_strings":["Medicine UMMC Jackson MS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medicine UMMC Jackson MS","institution_ids":["https://openalex.org/I29606459"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110786655","display_name":"Gregory V. Chinchar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Greg Chinchar","raw_affiliation_strings":["Microbiology","Pathology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microbiology","institution_ids":[]},{"raw_affiliation_string":"Pathology","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00231384,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"23","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10506","display_name":"Aquaculture disease management and microbiota","score":0.3386000096797943,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10506","display_name":"Aquaculture disease management and microbiota","score":0.3386000096797943,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10332","display_name":"Amphibian and Reptile Biology","score":0.11670000106096268,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11668","display_name":"interferon and immune responses","score":0.10209999978542328,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ranavirus","display_name":"ranavirus","score":0.878000020980835},{"id":"https://openalex.org/keywords/frog-virus-3","display_name":"frog virus 3","score":0.9729999899864197},{"id":"https://openalex.org/keywords/rna-interference","display_name":"RNA interference","score":0.8109999895095825},{"id":"https://openalex.org/keywords/morpholino-antisense","display_name":"morpholino antisense","score":0.9190000295639038},{"id":"https://openalex.org/keywords/gene-function","display_name":"gene function","score":0.8399999737739563},{"id":"https://openalex.org/keywords/viral-replication","display_name":"viral replication","score":0.8410000205039978},{"id":"https://openalex.org/keywords/pathogenesis","display_name":"pathogenesis","score":0.7049999833106995},{"id":"https://openalex.org/keywords/major-capsid-protein","display_name":"major capsid protein","score":0.9739999771118164},{"id":"https://openalex.org/keywords/virus-assembly","display_name":"viral assembly","score":0.8349999785423279},{"id":"https://openalex.org/keywords/cytopathic-effect","display_name":"cytopathic effect","score":0.8309999704360962},{"id":"https://openalex.org/keywords/poikilothermic-vertebrates","display_name":"poikilothermic vertebrates","score":0.7839999794960022},{"id":"https://openalex.org/keywords/sirna","display_name":"siRNA","score":0.6700000166893005}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.839536190032959},{"id":"https://openalex.org/C2780929364","wikidata":"https://www.wikidata.org/wiki/Q3418768","display_name":"Ranavirus","level":3,"score":0.7560625076293945},{"id":"https://openalex.org/C140704245","wikidata":"https://www.wikidata.org/wiki/Q3933202","display_name":"Viral replication","level":3,"score":0.5693132877349854},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5504103302955627},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5424382090568542},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.5071148872375488},{"id":"https://openalex.org/C140933497","wikidata":"https://www.wikidata.org/wiki/Q7933606","display_name":"Viral pathogenesis","level":4,"score":0.46462520956993103},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.4267400801181793},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.407633900642395},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2952636778354645}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.23.1_supplement.439.6","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.439.6","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.23.1_supplement.439.6","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.504800021648407,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2145915291","https://openalex.org/W7100832096","https://openalex.org/W2072953422","https://openalex.org/W2068424908","https://openalex.org/W2091218679","https://openalex.org/W2103951847","https://openalex.org/W6990005026","https://openalex.org/W2063342054","https://openalex.org/W3180414308","https://openalex.org/W2028868996"],"abstract_inverted_index":{"Viruses":[0],"of":[1,12,23,68,117,142,170,181,187],"the":[2,35,49,56,69,75,101,114,129,185],"genus":[3],"Ranavirus":[4],",":[5,8],"family":[6],"Iridoviridae":[7],"are":[9],"a":[10,179],"group":[11],"large,":[13],"icosahedral":[14],"dsDNA":[15],"viruses":[16],"which":[17,37],"have":[18,71,78],"emerged":[19],"as":[20,55,178],"new":[21],"pathogens":[22],"poikilothermic":[24],"vertebrates.":[25],"To":[26],"confront":[27],"their":[28],"spread,":[29],"it":[30],"is":[31],"vital":[32],"to":[33,95],"understand":[34],"mechanisms":[36],"control":[38],"virus":[39,46],"replication,":[40],"host\u2010range,":[41],"and":[42,66,89,99,107,128,147,168,183,193],"pathogenesis.":[43,108,194],"Therefore,":[44],"Frog":[45],"3":[47],"(FV3),":[48],"ranavirus":[50],"type":[51],"species,":[52],"was":[53,93],"chosen":[54,94],"model":[57],"system.":[58],"The":[59,109],"FV3":[60],"genome":[61],"has":[62],"been":[63],"completely":[64],"sequenced":[65],"~25%":[67],"genes":[70,188],"recognized":[72],"functions":[73],"whereas":[74],"remaining":[76],"~75%":[77],"no":[79],"known":[80],"function.":[81],"An":[82],"antisense":[83,90,176],"approach":[84],"using":[85,135],"RNA":[86,118],"interference":[87],"(RNAi)":[88],"morpholinos":[91],"(asMOs)":[92],"identify":[96],"gene":[97,136,145],"function":[98,186],"determine":[100],"requirements":[102],"for":[103],"replication":[104,192],"in":[105,144,149,157,165,190],"vitro":[106],"major":[110],"capsid":[111],"protein":[112,124],"(MCP),":[113],"viral":[115,150,191],"homologue":[116],"Polymerase":[119],"II":[120],"(vPol\u2010II\u03b1}),":[121],"myristolated":[122],"membrane":[123],"(MMP),":[125],"ICP\u201046":[126],"(46K),":[127],"cytosine":[130],"DNA\u2010methyltransferase":[131],"(DMT)":[132],"were":[133,152],"targeted":[134],"specific":[137],"siRNAs":[138],"or":[139],"asMOs.":[140],"Reductions":[141],">80%":[143],"expression":[146],">90%":[148],"titers":[151],"observed":[153],"along":[154],"with":[155],"reductions":[156,164],"cytopathic":[158],"effect.":[159],"Transmission":[160],"electron":[161],"microscopy":[162],"revealed":[163],"virion":[166],"assembly":[167],"production":[169],"atypical":[171],"elements.":[172],"These":[173],"results":[174],"support":[175],"techniques":[177],"way":[180],"identifying":[182],"elucidating":[184],"involved":[189]},"counts_by_year":[],"updated_date":"2026-09-27T08:07:27.782135","created_date":"2016-06-24T00:00:00"}