{"id":"https://openalex.org/W34876996","doi":null,"title":"Real-time calcium response of cultured bone cells to fluid flow.","display_name":"Real-time calcium response of cultured bone cells to fluid flow.","publication_year":1995,"publication_date":"1995-04-01","ids":{"openalex":"https://openalex.org/W34876996","mag":"34876996","pmid":"https://pubmed.ncbi.nlm.nih.gov/7641488"},"language":"en","primary_location":{"id":"pmid:7641488","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7641488","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical orthopaedics and related research","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5010048130","display_name":"Clark T. Hung","orcid":"https://orcid.org/0000-0002-7189-9477","observed_orcids":["https://orcid.org/0000-0002-7189-9477"]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C T Hung","raw_affiliation_strings":["Department of Bioengineering, University of Pennsylvania, Philadelphia 19104-6392, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, University of Pennsylvania, Philadelphia 19104-6392, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103559124","display_name":"Solomon R. Pollack","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S R Pollack","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"T M Reilly","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T M Reilly","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5043516951","display_name":"Carl T. Brighton","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C T Brighton","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.701,"has_fulltext":false,"cited_by_count":227,"citation_normalized_percentile":{"value":0.82659329,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":null,"issue":"313","first_page":"256","last_page":"69"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9458000063896179,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9458000063896179,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11219","display_name":"Bone Metabolism and Diseases","score":0.014100000262260437,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12005","display_name":"Spaceflight effects on biology","score":0.007199999876320362,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fluid-shear-stress","display_name":"fluid shear stress","score":0.7929999828338623},{"id":"https://openalex.org/keywords/intracellular-calcium","display_name":"intracellular calcium","score":0.8339999914169312},{"id":"https://openalex.org/keywords/bone-cells","display_name":"bone cells","score":0.75},{"id":"https://openalex.org/keywords/calvarial-osteoblasts","display_name":"calvarial osteoblasts","score":0.8420000076293945},{"id":"https://openalex.org/keywords/fluid-flow","display_name":"fluid flow","score":0.3790000081062317},{"id":"https://openalex.org/keywords/inositol-trisphosphate","display_name":"inositol trisphosphate","score":0.9300000071525574},{"id":"https://openalex.org/keywords/cell-heterogeneity","display_name":"cellular heterogeneity","score":0.6869999766349792},{"id":"https://openalex.org/keywords/dose-response-relationship","display_name":"dose-response relationship","score":0.515999972820282},{"id":"https://openalex.org/keywords/mechanotransduction","display_name":"mechanotransduction","score":0.5289999842643738}],"concepts":[{"id":"https://openalex.org/C519063684","wikidata":"https://www.wikidata.org/wiki/Q706","display_name":"Calcium","level":2,"score":0.6708178520202637},{"id":"https://openalex.org/C158617107","wikidata":"https://www.wikidata.org/wiki/Q79927","display_name":"Endoplasmic reticulum","level":2,"score":0.5685722827911377},{"id":"https://openalex.org/C21141959","wikidata":"https://www.wikidata.org/wiki/Q657936","display_name":"Shear stress","level":2,"score":0.5484977960586548},{"id":"https://openalex.org/C181080969","wikidata":"https://www.wikidata.org/wiki/Q60097","display_name":"Calcium in biology","level":3,"score":0.4949790835380554},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4929020404815674},{"id":"https://openalex.org/C133605539","wikidata":"https://www.wikidata.org/wiki/Q913236","display_name":"Bone cell","level":2,"score":0.48198002576828003},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.47601157426834106},{"id":"https://openalex.org/C2910081258","wikidata":"https://www.wikidata.org/wiki/Q177807","display_name":"Hydrostatic pressure","level":2,"score":0.45887529850006104},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.45549827814102173},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.3395726680755615},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3085523247718811},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.30261707305908203},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.16550588607788086},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.14631280303001404},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001842","descriptor_name":"Bone and Bones","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D001842","descriptor_name":"Bone and Bones","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D001842","descriptor_name":"Bone and Bones","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D001842","descriptor_name":"Bone and Bones","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001842","descriptor_name":"Bone and Bones","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001842","descriptor_name":"Bone and Bones","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007295","descriptor_name":"Inositol Phosphates","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D007295","descriptor_name":"Inositol Phosphates","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D007295","descriptor_name":"Inositol Phosphates","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010006","descriptor_name":"Osteoblasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010006","descriptor_name":"Osteoblasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010006","descriptor_name":"Osteoblasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010006","descriptor_name":"Osteoblasts","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D010006","descriptor_name":"Osteoblasts","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D010006","descriptor_name":"Osteoblasts","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D012212","descriptor_name":"Rheology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012212","descriptor_name":"Rheology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012212","descriptor_name":"Rheology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013314","descriptor_name":"Stress, Mechanical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013314","descriptor_name":"Stress, Mechanical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013314","descriptor_name":"Stress, Mechanical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017207","descriptor_name":"Rats, Sprague-Dawley","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017207","descriptor_name":"Rats, Sprague-Dawley","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017207","descriptor_name":"Rats, Sprague-Dawley","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:7641488","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7641488","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical orthopaedics and related research","raw_type":"Journal Article"},{"id":"mag:34876996","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7641488","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":null,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.8100000023841858,"id":"https://metadata.un.org/sdg/6","display_name":"Clean water and sanitation"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G116648535","display_name":"INTERACTION OF HORMONES AND PHYSICAL STRESS ON BONE MASS","funder_award_id":"5p50ar039226-03","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000888012","display_name":"Training Program in Musculoskeletal Research","funder_award_id":"5t32ar007132-49","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2040618621","https://openalex.org/W2168982181","https://openalex.org/W2004838499","https://openalex.org/W2747808735","https://openalex.org/W2035074158","https://openalex.org/W2070040557","https://openalex.org/W2091379972","https://openalex.org/W2089277316","https://openalex.org/W2617239383","https://openalex.org/W2030599914"],"abstract_inverted_index":{"Using":[0],"a":[1,149],"parallel-plate":[2],"flow":[3,173],"chamber":[4],"and":[5,27,42,61,81,115],"fura-2":[6],"fluorescence":[7],"microscopy,":[8],"intracellular":[9,141],"calcium":[10,139,155],"was":[11,44,67,159],"measured":[12],"cell":[13,15,97],"by":[14,90],"in":[16,122,130,175],"preconfluent":[17],"primary":[18],"culture":[19],"rat":[20],"calvarial":[21],"bone":[22,131,176],"cells":[23,74,86,105],"to":[24,39,93,100,125],"18,":[25],"35,":[26],"70":[28],"dynes/cm2":[29],"of":[30,59,72,103,138],"fluid-induced":[31],"shear":[32,117],"stress.":[33],"A":[34,64],"heterogeneous":[35],"response":[36,60,98,174],"with":[37,49,133,161],"respect":[38],"peak":[40,57],"amplitude":[41,58],"latency":[43],"observed":[45,68,96,153],"for":[46],"the":[47,55,70,123,136,145,171],"culture,":[48],"an":[50,166],"overriding":[51],"dose-dependent":[52],"relationship":[53],"between":[54,69],"mean":[56],"shear-stress":[62,82],"magnitude.":[63,83],"dose":[65],"dependence":[66],"number":[71],"responsive":[73],"(responding":[75],">":[76],"50%":[77],"over":[78],"basal":[79],"levels)":[80],"Not":[84],"all":[85],"could":[87],"be":[88,101],"restimulated":[89],"repeated":[91],"exposure":[92],"flow.":[94],"The":[95],"appears":[99],"independent":[102],"whether":[104],"are":[106],"clustered":[107],"together":[108],"or":[109],"isolated.":[110],"Substratum":[111],"stretch,":[112],"hydrostatic":[113],"pressure,":[114],"fluid":[116,172],"stress":[118],"have":[119],"been":[120],"shown":[121],"literature":[124],"increase":[126],"inositol":[127],"phosphate":[128],"(IP3)":[129],"cells,":[132],"IP3":[134,167],"causing":[135],"release":[137,156],"from":[140,157],"stores":[142,158],"such":[143],"as":[144],"endoplasmic":[146],"reticulum.":[147],"Therefore,":[148],"6-fold":[150],"inhibitory":[151],"effect":[152],"when":[154],"blocked":[160],"8-(n,N-diethylamino)octyl":[162],"1-3,4,5-atrimethoxybenzoate":[163],"hydrochloride":[164],"implicates":[165],"biochemical":[168],"pathway":[169],"mediating":[170],"cells.":[177]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":11},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":7},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":9}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2025-10-10T00:00:00"}