{"id":"https://openalex.org/W346937772","doi":null,"title":"Method Development for an Easy and Direct Quantitation of Protein Adsorption by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)","display_name":"Method Development for an Easy and Direct Quantitation of Protein Adsorption by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)","publication_year":2014,"publication_date":"2014-11-20","ids":{"openalex":"https://openalex.org/W346937772","mag":"346937772"},"language":"en","primary_location":{"id":"pmh:oai:summit.sfu.ca:14962","doi":null,"is_oa":true,"landing_page_url":"http://summit.sfu.ca/item/14962","pdf_url":"http://summit.sfu.ca/item/14962","source":{"id":"https://openalex.org/S4306400490","display_name":"Summit (Simon Fraser University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I18014758","host_organization_name":"Simon Fraser University","host_organization_lineage":["https://openalex.org/I18014758"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://summit.sfu.ca/item/14962","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5035502440","display_name":"Man Chung Gilbert Lee","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Man Chung Gilbert Lee","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5035502440"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.006500000134110451,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-adsorption","display_name":"protein adsorption","score":0.9959999918937683},{"id":"https://openalex.org/keywords/protein-quantification","display_name":"protein quantification","score":0.7210000157356262},{"id":"https://openalex.org/keywords/sample-loss","display_name":"sample loss","score":0.6029999852180481},{"id":"https://openalex.org/keywords/disease-biomarkers","display_name":"disease biomarkers","score":0.8709999918937683},{"id":"https://openalex.org/keywords/sample-preparation","display_name":"sample preparation","score":0.34299999475479126}],"concepts":[{"id":"https://openalex.org/C2778913249","wikidata":"https://www.wikidata.org/wiki/Q422241","display_name":"Sodium dodecyl sulfate","level":2,"score":0.7990257740020752},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.7707483768463135},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6802273988723755},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5916568636894226},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.5523949265480042},{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.5139000415802002},{"id":"https://openalex.org/C35859425","wikidata":"https://www.wikidata.org/wiki/Q384609","display_name":"Gel electrophoresis of proteins","level":4,"score":0.5028647780418396},{"id":"https://openalex.org/C40250595","wikidata":"https://www.wikidata.org/wiki/Q185098","display_name":"Electrophoresis","level":2,"score":0.46284645795822144},{"id":"https://openalex.org/C2778862431","wikidata":"https://www.wikidata.org/wiki/Q416002","display_name":"Polyacrylamide","level":2,"score":0.4505329132080078},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.4241657853126526},{"id":"https://openalex.org/C47793336","wikidata":"https://www.wikidata.org/wiki/Q4688061","display_name":"Two-dimensional gel electrophoresis","level":4,"score":0.41193538904190063},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.20126581192016602},{"id":"https://openalex.org/C188027245","wikidata":"https://www.wikidata.org/wiki/Q750446","display_name":"Polymer chemistry","level":1,"score":0.06678140163421631},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.05108082294464111},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.035124629735946655},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:summit.sfu.ca:14962","doi":null,"is_oa":true,"landing_page_url":"http://summit.sfu.ca/item/14962","pdf_url":"http://summit.sfu.ca/item/14962","source":{"id":"https://openalex.org/S4306400490","display_name":"Summit (Simon Fraser University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I18014758","host_organization_name":"Simon Fraser University","host_organization_lineage":["https://openalex.org/I18014758"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"pmh:oai:summit.sfu.ca:node-14962","doi":null,"is_oa":false,"landing_page_url":"https://summit.sfu.ca/_flysystem/fedora/sfu_migrate/14962/etd8754_MLee.pdf","pdf_url":"https://summit.sfu.ca/_flysystem/fedora/sfu_migrate/14962/etd8754_MLee.pdf","source":{"id":"https://openalex.org/S4306400490","display_name":"Summit (Simon Fraser University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I18014758","host_organization_name":"Simon Fraser University","host_organization_lineage":["https://openalex.org/I18014758"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},{"id":"mag:346937772","doi":null,"is_oa":false,"landing_page_url":"https://summit.sfu.ca/system/files/iritems1/14962/etd8754_MLee.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:summit.sfu.ca:14962","doi":null,"is_oa":true,"landing_page_url":"http://summit.sfu.ca/item/14962","pdf_url":"http://summit.sfu.ca/item/14962","source":{"id":"https://openalex.org/S4306400490","display_name":"Summit (Simon Fraser University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I18014758","host_organization_name":"Simon Fraser University","host_organization_lineage":["https://openalex.org/I18014758"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W346937772.pdf","grobid_xml":"https://content.openalex.org/works/W346937772.grobid-xml"},"referenced_works_count":141,"referenced_works":["https://openalex.org/W24237016","https://openalex.org/W42974555","https://openalex.org/W50359395","https://openalex.org/W162472049","https://openalex.org/W350472026","https://openalex.org/W1566254055","https://openalex.org/W1578154829","https://openalex.org/W1727368590","https://openalex.org/W1920608637","https://openalex.org/W1963641510","https://openalex.org/W1964327205","https://openalex.org/W1965304129","https://openalex.org/W1965516068","https://openalex.org/W1966591376","https://openalex.org/W1970467184","https://openalex.org/W1970513664","https://openalex.org/W1970731237","https://openalex.org/W1974467570","https://openalex.org/W1977032636","https://openalex.org/W1977078515","https://openalex.org/W1977182778","https://openalex.org/W1980240275","https://openalex.org/W1982229543","https://openalex.org/W1983255121","https://openalex.org/W1983706729","https://openalex.org/W1985011835","https://openalex.org/W1985676128","https://openalex.org/W1987507626","https://openalex.org/W1990270715","https://openalex.org/W1991366673","https://openalex.org/W1991770120","https://openalex.org/W1993363985","https://openalex.org/W1995582835","https://openalex.org/W1996251473","https://openalex.org/W1997848759","https://openalex.org/W1998866603","https://openalex.org/W1999460433","https://openalex.org/W2001482471","https://openalex.org/W2003261719","https://openalex.org/W2003670177","https://openalex.org/W2004965765","https://openalex.org/W2005593738","https://openalex.org/W2007681573","https://openalex.org/W2007808060","https://openalex.org/W2008275763","https://openalex.org/W2008309511","https://openalex.org/W2009235113","https://openalex.org/W2009340358","https://openalex.org/W2009589847","https://openalex.org/W2009778396","https://openalex.org/W2011162762","https://openalex.org/W2012651880","https://openalex.org/W2014536520","https://openalex.org/W2014741634","https://openalex.org/W2017694438","https://openalex.org/W2020243385","https://openalex.org/W2021264738","https://openalex.org/W2021370964","https://openalex.org/W2021496896","https://openalex.org/W2023361213","https://openalex.org/W2027100614","https://openalex.org/W2027195682","https://openalex.org/W2030370943","https://openalex.org/W2032986905","https://openalex.org/W2036332397","https://openalex.org/W2037199116","https://openalex.org/W2038245901","https://openalex.org/W2039108689","https://openalex.org/W2039111594","https://openalex.org/W2040343906","https://openalex.org/W2040576110","https://openalex.org/W2042018302","https://openalex.org/W2043201241","https://openalex.org/W2043807407","https://openalex.org/W2043862933","https://openalex.org/W2043951056","https://openalex.org/W2044252957","https://openalex.org/W2044350423","https://openalex.org/W2044936649","https://openalex.org/W2047358993","https://openalex.org/W2048386842","https://openalex.org/W2048806783","https://openalex.org/W2049304826","https://openalex.org/W2050343840","https://openalex.org/W2051809212","https://openalex.org/W2052100687","https://openalex.org/W2059160347","https://openalex.org/W2060259421","https://openalex.org/W2063669910","https://openalex.org/W2063932359","https://openalex.org/W2064940913","https://openalex.org/W2065843914","https://openalex.org/W2067137241","https://openalex.org/W2070514988","https://openalex.org/W2070685019","https://openalex.org/W2072641355","https://openalex.org/W2072766894","https://openalex.org/W2077085156","https://openalex.org/W2077888386","https://openalex.org/W2078594804","https://openalex.org/W2079273397","https://openalex.org/W2080014512","https://openalex.org/W2080690891","https://openalex.org/W2083840814","https://openalex.org/W2084069730","https://openalex.org/W2085316659","https://openalex.org/W2086608330","https://openalex.org/W2087481305","https://openalex.org/W2090739820","https://openalex.org/W2091746443","https://openalex.org/W2091836140","https://openalex.org/W2093477515","https://openalex.org/W2102642012","https://openalex.org/W2106376891","https://openalex.org/W2110114053","https://openalex.org/W2110176833","https://openalex.org/W2115640378","https://openalex.org/W2118177631","https://openalex.org/W2120919608","https://openalex.org/W2121800230","https://openalex.org/W2123960992","https://openalex.org/W2126966136","https://openalex.org/W2128252450","https://openalex.org/W2138373796","https://openalex.org/W2140886928","https://openalex.org/W2140985419","https://openalex.org/W2149412239","https://openalex.org/W2155027540","https://openalex.org/W2158359119","https://openalex.org/W2164455066","https://openalex.org/W2166308139","https://openalex.org/W2166691093","https://openalex.org/W2172237901","https://openalex.org/W2312983840","https://openalex.org/W2314894294","https://openalex.org/W2317513811","https://openalex.org/W2324828024","https://openalex.org/W2333064713","https://openalex.org/W2476523197","https://openalex.org/W2502949873","https://openalex.org/W2949167950"],"related_works":["https://openalex.org/W2009778396","https://openalex.org/W2920105708","https://openalex.org/W2793251318","https://openalex.org/W2301785349","https://openalex.org/W2153928015","https://openalex.org/W2088211136","https://openalex.org/W2056777570","https://openalex.org/W2144046187","https://openalex.org/W2593781313","https://openalex.org/W21830548"],"abstract_inverted_index":{"The":[0],"current":[1],"proteomic":[2],"approach":[3],"is":[4,56,131],"to":[5,31,96,112],"identify":[6],"and":[7,69,103,120],"analyze":[8],"proteins":[9,21,82],"of":[10,62,81,100,127,139],"interest,":[11],"which":[12],"can":[13],"potentially":[14],"be":[15],"disease":[16],"biomarkers.":[17],"However,":[18],"these":[19],"important":[20],"usually":[22],"exist":[23],"in":[24,117,123],"very":[25],"low":[26,88],"concentration,":[27,101],"making":[28],"them":[29],"vulnerable":[30],"sample":[32,115],"loss":[33,116],"by":[34],"adsorption":[35,53],"during":[36],"any":[37],"laboratory":[38],"manipulations.":[39],"A":[40],"simple":[41],"method,":[42],"sodium":[43],"dodecyl":[44],"sulfate":[45],"polyacrylamide":[46],"gel":[47],"electrophoresis":[48],"coupled":[49],"with":[50],"direct":[51],"protein":[52,64,77,118],"analysis":[54],"(SDS-PAGE/DPA),":[55],"presented":[57],"here":[58],"for":[59,76,135],"the":[60,98],"quantitation":[61],"adsorption-caused":[63,140],"loss.":[65,142],"No":[66],"complicated":[67],"steps":[68],"expensive":[70],"equipment":[71],"are":[72],"involved;":[73],"this":[74,94,110],"method":[75,95,111,130],"study":[78],"enables":[79],"measurement":[80],"adsorbed":[83],"on":[84,105],"vials":[85],"at":[86],"extremely":[87],"concentrations":[89],"(in":[90],"pg/\u03bcl).":[91],"We":[92,107],"used":[93],"characterize":[97],"effects":[99],"time,":[102],"volume":[104],"adsorption.":[106,128],"also":[108],"applied":[109],"discover":[113],"differential":[114],"mixtures":[119],"its":[121],"utility":[122],"developing":[124],"preventive":[125],"strategies":[126],"This":[129],"further":[132],"being":[133],"employed":[134],"studying":[136],"different":[137],"aspects":[138],"peptide":[141]},"counts_by_year":[{"year":2017,"cited_by_count":1}],"updated_date":"2026-08-30T07:30:35.949966","created_date":"2016-06-24T00:00:00"}