{"id":"https://openalex.org/W345935162","doi":null,"title":"Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization.","display_name":"Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization.","publication_year":1987,"publication_date":"1987-06-01","ids":{"openalex":"https://openalex.org/W345935162","mag":"345935162","pmid":"https://pubmed.ncbi.nlm.nih.gov/3035929","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1899767"},"language":"en","primary_location":{"id":"pmh:oai:pubmedcentral.nih.gov:1899767","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1899767","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Am J Pathol","raw_type":"Text"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1899767","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5004256523","display_name":"Edward Seto","orcid":"https://orcid.org/0000-0001-7562-7316","observed_orcids":["https://orcid.org/0000-0001-7562-7316"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"E Seto","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":null,"display_name":"T S Yen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T S Yen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.7149,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.89160598,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"127","issue":"3","first_page":"409","last_page":"13"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10981","display_name":"Cytomegalovirus and herpesvirus research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10981","display_name":"Cytomegalovirus and herpesvirus research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10625","display_name":"Herpesvirus Infections and Treatments","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10685","display_name":"Viral-associated cancers and disorders","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cytomegalovirus","display_name":"cytomegalovirus","score":0.9079999923706055},{"id":"https://openalex.org/keywords/cmv-dna-detection","display_name":"CMV DNA detection","score":0.871999979019165},{"id":"https://openalex.org/keywords/paraffin-embedded-tissue","display_name":"paraffin-embedded tissue","score":0.9819999933242798},{"id":"https://openalex.org/keywords/dot-blot-hybridization","display_name":"dot blot","score":0.8320000171661377},{"id":"https://openalex.org/keywords/formalin-fixed-tissue","display_name":"formalin-fixed tissue","score":0.8090000152587891},{"id":"https://openalex.org/keywords/biotinylated-dna-probes","display_name":"biotinylated DNA probes","score":0.9610000252723694},{"id":"https://openalex.org/keywords/diagnostic-sensitivity","display_name":"diagnostic sensitivity","score":0.6589999794960022},{"id":"https://openalex.org/keywords/viral-diagnosis","display_name":"viral diagnosis","score":0.5320000052452087}],"concepts":[{"id":"https://openalex.org/C2777732132","wikidata":"https://www.wikidata.org/wiki/Q6946","display_name":"Cytomegalovirus","level":5,"score":0.6981604695320129},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.682794988155365},{"id":"https://openalex.org/C204628709","wikidata":"https://www.wikidata.org/wiki/Q864854","display_name":"Biotinylation","level":2,"score":0.6599791646003723},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5834895968437195},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5685407519340515},{"id":"https://openalex.org/C130197536","wikidata":"https://www.wikidata.org/wiki/Q418436","display_name":"Hybridization probe","level":3,"score":0.5681284666061401},{"id":"https://openalex.org/C54871578","wikidata":"https://www.wikidata.org/wiki/Q2074275","display_name":"Nucleic acid thermodynamics","level":4,"score":0.5644176006317139},{"id":"https://openalex.org/C2776409557","wikidata":"https://www.wikidata.org/wiki/Q149348","display_name":"Herpesviridae","level":4,"score":0.5077956318855286},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5009589195251465},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.4854481816291809},{"id":"https://openalex.org/C2777662283","wikidata":"https://www.wikidata.org/wiki/Q747711","display_name":"Betaherpesvirinae","level":5,"score":0.4536246657371521},{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.4426548480987549},{"id":"https://openalex.org/C137390916","wikidata":"https://www.wikidata.org/wiki/Q900923","display_name":"DNA\u2013DNA hybridization","level":3,"score":0.4375995695590973},{"id":"https://openalex.org/C2780727368","wikidata":"https://www.wikidata.org/wiki/Q1928978","display_name":"Viral disease","level":3,"score":0.2709317207336426},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1053132712841034},{"id":"https://openalex.org/C3017666073","wikidata":"https://www.wikidata.org/wiki/Q1764062","display_name":"Base sequence","level":3,"score":0.09699156880378723},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.06304758787155151},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.05920678377151489}],"mesh":[{"descriptor_ui":"D001344","descriptor_name":"Autopsy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001706","descriptor_name":"Biopsy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003586","descriptor_name":"Cytomegalovirus Infections","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D003586","descriptor_name":"Cytomegalovirus Infections","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003587","descriptor_name":"Cytomegalovirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D005404","descriptor_name":"Fixatives","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009693","descriptor_name":"Nucleic Acid Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010232","descriptor_name":"Paraffin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"pmh:oai:pubmedcentral.nih.gov:1899767","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1899767","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Am J Pathol","raw_type":"Text"},{"id":"pmid:3035929","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3035929","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The American journal of pathology","raw_type":"Journal Article"},{"id":"mag:345935162","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3035929","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:1899767","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1899767","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Am J Pathol","raw_type":"Text"},"sustainable_development_goals":[{"score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W1790066431","https://openalex.org/W1902188623","https://openalex.org/W1969845182","https://openalex.org/W1980147683","https://openalex.org/W1998649191","https://openalex.org/W2008354532","https://openalex.org/W2014343505","https://openalex.org/W2037098216","https://openalex.org/W2050717506","https://openalex.org/W2331428177"],"related_works":["https://openalex.org/W1973328890","https://openalex.org/W2059031603","https://openalex.org/W304133910","https://openalex.org/W2058491192","https://openalex.org/W2066960430","https://openalex.org/W2331428177","https://openalex.org/W2014059118","https://openalex.org/W2078359567","https://openalex.org/W1966764305","https://openalex.org/W1588278006"],"abstract_inverted_index":{"A":[0],"specific":[1],"and":[2,95],"rapid":[3],"method":[4,74],"for":[5],"detecting":[6],"the":[7,32,47,50,100],"presence":[8,33],"of":[9,34,46,64,75,102],"cytomegalovirus":[10],"(CMV)":[11],"DNA":[12,30],"in":[13,49,93],"formalin-":[14],"or":[15],"mercuric":[16],"chloride-fixed,":[17],"paraffin-embedded":[18],"tissue":[19,51],"has":[20],"been":[21],"developed.":[22],"With":[23],"dot":[24],"hybridization":[25],"with":[26],"a":[27,44],"biotinylated":[28],"CMV":[29,35],"probe,":[31],"infection":[36],"can":[37],"be":[38,85,97],"readily":[39],"detected":[40],"even":[41],"if":[42,66],"only":[43],"minority":[45],"cells":[48],"block":[52],"are":[53],"infected.":[54],"The":[55],"sensitivity":[56],"is":[57,71,77],"further":[58],"increased":[59],"by":[60,90],"about":[61],"two":[62],"orders":[63],"magnitude":[65],"high":[67],"specific-activity":[68],"32P-labeled":[69],"probe":[70],"used.":[72],"This":[73],"detection":[76,101],"more":[78],"sensitive":[79],"than":[80],"histologic":[81],"diagnosis,":[82],"appears":[83],"to":[84,88,99],"roughly":[86],"comparable":[87],"that":[89],"virus":[91],"isolation":[92],"culture,":[94],"should":[96],"applicable":[98],"other":[103],"infectious":[104],"agents.":[105]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}