{"id":"https://openalex.org/W338277601","doi":"https://doi.org/10.3791/52836-v","title":"In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA","display_name":"In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA","publication_year":2015,"publication_date":"2015-05-21","ids":{"openalex":"https://openalex.org/W338277601","doi":"https://doi.org/10.3791/52836-v","mag":"338277601","pmid":"https://pubmed.ncbi.nlm.nih.gov/26066790","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4542956"},"language":"en","primary_location":{"id":"doi:10.3791/52836-v","doi":"https://doi.org/10.3791/52836-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/52836-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jove.com/pdf/52836/in-situ-detection-bacteria-within-paraffin-embedded-tissues-using","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040448932","display_name":"Yun Sik Choi","orcid":"https://orcid.org/0000-0002-1321-8119","observed_orcids":["https://orcid.org/0000-0002-1321-8119"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Yun Sik Choi","raw_affiliation_strings":["Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University","Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University; youngnim@snu.ac.kr"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University","institution_ids":["https://openalex.org/I139264467"]},{"raw_affiliation_string":"Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University; youngnim@snu.ac.kr","institution_ids":["https://openalex.org/I139264467"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060501268","display_name":"Yong Cheol Kim","orcid":"https://orcid.org/0000-0002-2124-427X","observed_orcids":["https://orcid.org/0000-0002-2124-427X"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Yong Cheol Kim","raw_affiliation_strings":["Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University","institution_ids":["https://openalex.org/I139264467"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113501239","display_name":"Keum Jin Baek","orcid":"https://orcid.org/0000-0002-4849-0083","observed_orcids":["https://orcid.org/0000-0002-4849-0083"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Keum Jin Baek","raw_affiliation_strings":["Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University","institution_ids":["https://openalex.org/I139264467"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013683329","display_name":"Youngnim Choi","orcid":"https://orcid.org/0000-0002-6496-5560","observed_orcids":["https://orcid.org/0000-0002-6496-5560"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Youngnim Choi","raw_affiliation_strings":["Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University","Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University; youngnim@snu.ac.kr"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University","institution_ids":["https://openalex.org/I139264467"]},{"raw_affiliation_string":"Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University; youngnim@snu.ac.kr","institution_ids":["https://openalex.org/I139264467"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I139264467"],"apc_list":null,"apc_paid":null,"fwci":2.0847,"has_fulltext":true,"cited_by_count":17,"citation_normalized_percentile":{"value":0.84205979,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":null,"issue":"99","first_page":"e52836","last_page":"e52836"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10257","display_name":"Oral microbiology and periodontitis research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/3506","display_name":"Periodontics"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10257","display_name":"Oral microbiology and periodontitis research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/3506","display_name":"Periodontics"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13768","display_name":"Oral and gingival health research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/3506","display_name":"Periodontics"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10066","display_name":"Gut microbiota and health","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/16s-rrna","display_name":"16S rRNA","score":0.9449999928474426},{"id":"https://openalex.org/keywords/periodontitis","display_name":"periodontitis","score":0.8299999833106995},{"id":"https://openalex.org/keywords/bacterial-morphology","display_name":"bacterial morphology","score":0.5149999856948853},{"id":"https://openalex.org/keywords/bacterial-localization","display_name":"bacterial localization","score":0.37400001287460327},{"id":"https://openalex.org/keywords/periodontal-pathogens","display_name":"periodontal pathogens","score":0.6060000061988831},{"id":"https://openalex.org/keywords/immunohistochemistry","display_name":"immunohistochemistry","score":0.6050000190734863}],"concepts":[{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.7302287817001343},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.7115025520324707},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.6533746719360352},{"id":"https://openalex.org/C518705261","wikidata":"https://www.wikidata.org/wiki/Q25615","display_name":"Connective tissue","level":2,"score":0.6204013228416443},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5499446392059326},{"id":"https://openalex.org/C111824103","wikidata":"https://www.wikidata.org/wiki/Q7086828","display_name":"Oligomer restriction","level":4,"score":0.5429953336715698},{"id":"https://openalex.org/C2778236600","wikidata":"https://www.wikidata.org/wiki/Q592324","display_name":"Immunofluorescence","level":3,"score":0.5383573174476624},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.49094918370246887},{"id":"https://openalex.org/C130197536","wikidata":"https://www.wikidata.org/wiki/Q418436","display_name":"Hybridization probe","level":3,"score":0.44374072551727295},{"id":"https://openalex.org/C2777822432","wikidata":"https://www.wikidata.org/wiki/Q216681","display_name":"In situ","level":2,"score":0.44093188643455505},{"id":"https://openalex.org/C2777542201","wikidata":"https://www.wikidata.org/wiki/Q1336182","display_name":"Fluorescence in situ hybridization","level":4,"score":0.4183503985404968},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3780704140663147},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.35821470618247986},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.34133094549179077},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.32238054275512695},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.29800933599472046},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.22340205311775208},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.12574651837348938},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10321515798568726},{"id":"https://openalex.org/C30481170","wikidata":"https://www.wikidata.org/wiki/Q37748","display_name":"Chromosome","level":3,"score":0.09804052114486694},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.09786805510520935},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0930449366569519},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D004077","descriptor_name":"Digoxin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008010","descriptor_name":"Lichen Planus","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D008175","descriptor_name":"Lung Neoplasms","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D008807","descriptor_name":"Mice, Inbred BALB C","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015342","descriptor_name":"DNA Probes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015342","descriptor_name":"DNA Probes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D016480","descriptor_name":"Helicobacter pylori","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D016612","descriptor_name":"Paraffin Embedding","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016966","descriptor_name":"Porphyromonas gingivalis","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D017404","descriptor_name":"In Situ Hybridization, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":6,"locations":[{"id":"doi:10.3791/52836-v","doi":"https://doi.org/10.3791/52836-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/52836-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"doi:10.3791/52836","doi":"https://doi.org/10.3791/52836","is_oa":true,"landing_page_url":"https://doi.org/10.3791/52836","pdf_url":"https://www.jove.com/pdf/52836/in-situ-detection-bacteria-within-paraffin-embedded-tissues-using","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:4286968","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4542956","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4542956","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4542956","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Vis Exp","raw_type":"Text"},{"id":"pmid:26066790","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26066790","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"},{"id":"pmh:oai:s-space.snu.ac.kr:10371/138644","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/10371/138644","pdf_url":null,"source":{"id":"https://openalex.org/S4306401345","display_name":"Seoul National University Open Repository (Seoul National University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I139264467","host_organization_name":"Seoul National University","host_organization_lineage":["https://openalex.org/I139264467"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Article"}],"best_oa_location":{"id":"doi:10.3791/52836","doi":"https://doi.org/10.3791/52836","is_oa":true,"landing_page_url":"https://doi.org/10.3791/52836","pdf_url":"https://www.jove.com/pdf/52836/in-situ-detection-bacteria-within-paraffin-embedded-tissues-using","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8048999905586243}],"study_designs":[],"awards":[{"id":"https://openalex.org/G5395450484","display_name":null,"funder_award_id":"2013R1A1A3005669","funder_id":"https://openalex.org/F4320322120","funder_display_name":"National Research Foundation of Korea"}],"funders":[{"id":"https://openalex.org/F4320320671","display_name":"National Research Foundation","ror":"https://ror.org/05s0g1g46"},{"id":"https://openalex.org/F4320322120","display_name":"National Research Foundation of Korea","ror":"https://ror.org/013aysd81"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W338277601.pdf","grobid_xml":"https://content.openalex.org/works/W338277601.grobid-xml"},"referenced_works_count":18,"referenced_works":["https://openalex.org/W162831375","https://openalex.org/W1790944182","https://openalex.org/W1976913432","https://openalex.org/W1995462159","https://openalex.org/W1999564887","https://openalex.org/W2019533305","https://openalex.org/W2020981992","https://openalex.org/W2029287600","https://openalex.org/W2036647435","https://openalex.org/W2054023957","https://openalex.org/W2090443712","https://openalex.org/W2100925800","https://openalex.org/W2117397238","https://openalex.org/W2126227426","https://openalex.org/W2146483592","https://openalex.org/W2160573033","https://openalex.org/W2322162516","https://openalex.org/W4252700583"],"related_works":["https://openalex.org/W7127142242","https://openalex.org/W2124755699","https://openalex.org/W1923409245","https://openalex.org/W2980562432","https://openalex.org/W6942354177","https://openalex.org/W2022261635","https://openalex.org/W2420789479","https://openalex.org/W2076038689","https://openalex.org/W2114929859","https://openalex.org/W7046359296"],"abstract_inverted_index":{"The":[0,74,119],"presence":[1],"of":[2,82,114,149],"bacteria":[3,63,115,150],"within":[4,64],"the":[5,78,128,147],"pocket":[6],"epithelium":[7],"and":[8,34,111,134,158],"underlying":[9],"connective":[10,130],"tissue":[11],"in":[12,36,94,151],"gingival":[13],"biopsies":[14],"from":[15,86],"patients":[16],"with":[17],"periodontitis":[18],"has":[19,71],"been":[20,72],"reported":[21],"using":[22,31,40,67],"various":[23,152],"methods,":[24],"including":[25],"electron":[26],"microscopy,":[27],"immunohistochemistry":[28],"or":[29,56,97,116],"immunofluorescence":[30],"bacteria-specific":[32],"antibodies,":[33],"fluorescent":[35],"situ":[37],"hybridization":[38],"(FISH)":[39],"a":[41,59,95],"fluorescence-labeled":[42],"oligonucleotide":[43],"probe.":[44],"Nevertheless,":[45],"these":[46],"methods":[47],"are":[48,77,102,137],"not":[49],"widely":[50],"used":[51,144],"due":[52],"to":[53,61,125,145],"technical":[54],"limitation":[55],"difficulties.":[57],"Here":[58],"method":[60,141],"localize":[62],"paraffin-embedded":[65,75],"tissues":[66,76,84],"DIG-labeled":[68],"DNA":[69],"probes":[70],"introduced.":[73],"most":[79],"common":[80],"form":[81],"biopsy":[83],"available":[85],"pathology":[87],"banks.":[88],"Bacteria":[89],"can":[90,142],"be":[91,126,143],"detected":[92,103],"either":[93,105],"species-specific":[96],"universal":[98],"manner.":[99],"Bacterial":[100],"signals":[101],"as":[104,155],"discrete":[106],"forms":[107],"(coccus,":[108],"rod,":[109],"fusiform,":[110],"hairy":[112],"form)":[113],"dispersed":[117],"forms.":[118],"technique":[120],"allows":[121],"other":[122],"histological":[123],"information":[124],"obtained:":[127],"epithelia,":[129],"tissue,":[131],"inflammatory":[132,159],"infiltrates,":[133],"blood":[135],"vessels":[136],"well":[138],"distinguished.":[139],"This":[140],"study":[146],"role":[148],"diseases,":[153],"such":[154],"periodontitis,":[156],"cancers,":[157],"immune":[160],"diseases.":[161]},"counts_by_year":[{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":3}],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}