{"id":"https://openalex.org/W335697497","doi":"https://doi.org/10.1182/blood.v59.6.1310.1310","title":"Factor VIII/von Willebrand factor protein: sensitivity of periodic acid Schiff stain to carbohydrate deficiency","display_name":"Factor VIII/von Willebrand factor protein: sensitivity of periodic acid Schiff stain to carbohydrate deficiency","publication_year":1982,"publication_date":"1982-06-01","ids":{"openalex":"https://openalex.org/W335697497","doi":"https://doi.org/10.1182/blood.v59.6.1310.1310","mag":"335697497","pmid":"https://pubmed.ncbi.nlm.nih.gov/6805533"},"language":"en","primary_location":{"id":"doi:10.1182/blood.v59.6.1310.1310","doi":"https://doi.org/10.1182/blood.v59.6.1310.1310","is_oa":true,"landing_page_url":"https://doi.org/10.1182/blood.v59.6.1310.1310","pdf_url":"https://ashpublications.org/blood/article-pdf/59/6/1310/588317/1310.pdf","source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://ashpublications.org/blood/article-pdf/59/6/1310/588317/1310.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5114007397","display_name":"HR Gralnick","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"HR Gralnick","raw_affiliation_strings":["From the Hematology Service. Clinical Pathology Department, National Institutes of Health, Bethesda. Md"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Hematology Service. Clinical Pathology Department, National Institutes of Health, Bethesda. Md","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":null,"display_name":"GM Jackson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"GM Jackson","raw_affiliation_strings":["From the Hematology Service. Clinical Pathology Department, National Institutes of Health, Bethesda. Md"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Hematology Service. Clinical Pathology Department, National Institutes of Health, Bethesda. Md","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083369831","display_name":"SB Williams","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"SB Williams","raw_affiliation_strings":["From the Hematology Service. Clinical Pathology Department, National Institutes of Health, Bethesda. Md"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Hematology Service. Clinical Pathology Department, National Institutes of Health, Bethesda. Md","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062138446","display_name":"MC Cregger","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"MC Cregger","raw_affiliation_strings":["From the Hematology Service. Clinical Pathology Department, National Institutes of Health, Bethesda. Md"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Hematology Service. Clinical Pathology Department, National Institutes of Health, Bethesda. Md","institution_ids":["https://openalex.org/I1299303238"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1299303238"],"apc_list":{"value":5500,"currency":"USD","value_usd":5500},"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":4,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"59","issue":"6","first_page":"1310","last_page":"1316"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11280","display_name":"Hemophilia Treatment and Research","score":0.8148999810218811,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11280","display_name":"Hemophilia Treatment and Research","score":0.8148999810218811,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10746","display_name":"Platelet Disorders and Treatments","score":0.13490000367164612,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.03709999844431877,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/factor-viii","display_name":"factor VIII","score":0.8379999995231628},{"id":"https://openalex.org/keywords/von-willebrand-factor","display_name":"von Willebrand factor","score":1.0},{"id":"https://openalex.org/keywords/periodic-acid-schiff-staining","display_name":"PAS staining","score":0.8339999914169312},{"id":"https://openalex.org/keywords/coomassie-staining","display_name":"Coomassie staining","score":0.796999990940094},{"id":"https://openalex.org/keywords/carbohydrate-deficiency","display_name":"carbohydrate deficiency","score":0.7409999966621399},{"id":"https://openalex.org/keywords/desialylation","display_name":"desialylation","score":0.7870000004768372},{"id":"https://openalex.org/keywords/n-acetylglucosamine","display_name":"N-acetylglucosamine","score":0.9990000128746033},{"id":"https://openalex.org/keywords/sialic-acid","display_name":"sialic acid","score":0.972000002861023},{"id":"https://openalex.org/keywords/protein-multimers","display_name":"protein multimers","score":0.8040000200271606},{"id":"https://openalex.org/keywords/glycosylation","display_name":"glycosylation","score":0.7089999914169312},{"id":"https://openalex.org/keywords/hemophilia","display_name":"hemophilia","score":0.5590000152587891}],"concepts":[{"id":"https://openalex.org/C2780761556","wikidata":"https://www.wikidata.org/wiki/Q418516","display_name":"Coomassie Brilliant Blue","level":3,"score":0.7478488683700562},{"id":"https://openalex.org/C2780115458","wikidata":"https://www.wikidata.org/wiki/Q419311","display_name":"Sialic acid","level":2,"score":0.7465581893920898},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6956448554992676},{"id":"https://openalex.org/C2779394231","wikidata":"https://www.wikidata.org/wiki/Q412310","display_name":"Von Willebrand factor","level":3,"score":0.6675096750259399},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.6296356320381165},{"id":"https://openalex.org/C75786782","wikidata":"https://www.wikidata.org/wiki/Q1391262","display_name":"Periodic acid\u2013Schiff stain","level":3,"score":0.519309937953949},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.48058509826660156},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3937869966030121},{"id":"https://openalex.org/C89560881","wikidata":"https://www.wikidata.org/wiki/Q101026","display_name":"Platelet","level":2,"score":0.188703715801239},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.16594895720481873},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.07933041453361511},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001779","descriptor_name":"Blood Coagulation Factors","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D002241","descriptor_name":"Carbohydrates","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D005169","descriptor_name":"Factor VIII","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D006651","descriptor_name":"Histocytochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010503","descriptor_name":"Periodic Acid-Schiff Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012394","descriptor_name":"Rosaniline Dyes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012794","descriptor_name":"Sialic Acids","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D014841","descriptor_name":"von Willebrand Factor","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1182/blood.v59.6.1310.1310","doi":"https://doi.org/10.1182/blood.v59.6.1310.1310","is_oa":true,"landing_page_url":"https://doi.org/10.1182/blood.v59.6.1310.1310","pdf_url":"https://ashpublications.org/blood/article-pdf/59/6/1310/588317/1310.pdf","source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},{"id":"doi:10.1182/blood.v59.6.1310.bloodjournal5961310","doi":"https://doi.org/10.1182/blood.v59.6.1310.bloodjournal5961310","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v59.6.1310.bloodjournal5961310","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},{"id":"pmid:6805533","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6805533","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1182/blood.v59.6.1310.1310","doi":"https://doi.org/10.1182/blood.v59.6.1310.1310","is_oa":true,"landing_page_url":"https://doi.org/10.1182/blood.v59.6.1310.1310","pdf_url":"https://ashpublications.org/blood/article-pdf/59/6/1310/588317/1310.pdf","source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.7246000170707703,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W335697497.pdf","grobid_xml":"https://content.openalex.org/works/W335697497.grobid-xml"},"referenced_works_count":20,"referenced_works":["https://openalex.org/W1526076455","https://openalex.org/W1580023435","https://openalex.org/W1585764350","https://openalex.org/W1589732206","https://openalex.org/W1775749144","https://openalex.org/W1965846023","https://openalex.org/W1967882894","https://openalex.org/W1970458194","https://openalex.org/W1990790360","https://openalex.org/W1992257865","https://openalex.org/W2046054203","https://openalex.org/W2065133556","https://openalex.org/W2073720849","https://openalex.org/W2082697150","https://openalex.org/W2123125920","https://openalex.org/W2204334573","https://openalex.org/W2339860575","https://openalex.org/W2342796094","https://openalex.org/W2461972766","https://openalex.org/W2916193017"],"related_works":["https://openalex.org/W2082697150","https://openalex.org/W2342796094","https://openalex.org/W2945868031","https://openalex.org/W2945915356","https://openalex.org/W2946579561","https://openalex.org/W2077806825","https://openalex.org/W1967882894","https://openalex.org/W1992257865","https://openalex.org/W2139376868","https://openalex.org/W2465863028"],"abstract_inverted_index":{"We":[0],"have":[1,101],"investigated":[2],"the":[3,12,60,67,81,90,98,119,128,133,137],"periodic":[4],"acid":[5,105,121],"Schiff":[6],"(PAS)":[7],"Coomassie":[8],"staining":[9,22,108],"ratio":[10,23,31],"of":[11,32,57,72,92,97,132],"human":[13],"factor":[14,17],"VIII/von":[15],"Willebrand":[16],"(fVIII/vWf)":[18],"protein.":[19,62],"The":[20,29,47,94],"PAS-Coomassie":[21,30,68,82,107],"is":[24],"consistent":[25],"over":[26],"8":[27],"days.":[28],"fVIII/vWf":[33,61,99,139],"protein":[34,100],"purified":[35],"from":[36],"different":[37],"starting":[38],"materials":[39],"does":[40,112],"not":[41,65,113],"appear":[42,114],"to":[43,115,118,127],"be":[44,116,125],"significantly":[45],"different.":[46],"PAS":[48],"stain":[49],"can":[50],"detect":[51],"as":[52,54],"little":[53],"300":[55],"ng":[56],"carbohydrate":[58,134],"in":[59,76,80],"Desialation":[63],"did":[64],"affect":[66],"ratio,":[69],"while":[70],"removal":[71,91],"penultimate":[73],"galactose":[74],"resulted":[75],"a":[77,102],"marked":[78],"reduction":[79,85],"ratio.":[83,109],"This":[84,110],"was":[86],"further":[87],"accentuated":[88],"with":[89],"N-acetylglucosamine.":[93],"smaller":[95,138],"multimers":[96],"reduced":[103],"sialic":[104,120],"and":[106],"difference":[111],"related":[117,126],"deficiency":[122],"but":[123],"may":[124],"distribution":[129],"or":[130],"organization":[131],"moieties":[135],"on":[136],"multimers.":[140]},"counts_by_year":[{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}