{"id":"https://openalex.org/W33309701","doi":"https://doi.org/10.1038/npre.2008.1978.1","title":"Structural basis of the allosteric trigger of the Hsp70 chaperone proteins.","display_name":"Structural basis of the allosteric trigger of the Hsp70 chaperone proteins.","publication_year":2008,"publication_date":"2008-06-18","ids":{"openalex":"https://openalex.org/W33309701","doi":"https://doi.org/10.1038/npre.2008.1978.1","mag":"33309701"},"language":"en","primary_location":{"id":"doi:10.1038/npre.2008.1978.1","doi":"https://doi.org/10.1038/npre.2008.1978.1","is_oa":true,"landing_page_url":"https://doi.org/10.1038/npre.2008.1978.1","pdf_url":"https://www.nature.com/articles/npre.2008.1978.1.pdf","source":{"id":"https://openalex.org/S48744551","display_name":"Nature Precedings","issn_l":"1756-0357","issn":["1756-0357"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Precedings","raw_type":"journal-article"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://www.nature.com/articles/npre.2008.1978.1.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5077172319","display_name":"Akash Bhattacharya","orcid":"https://orcid.org/0000-0001-8748-9969","observed_orcids":["https://orcid.org/0000-0001-8748-9969"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Akash Bhattacharya","raw_affiliation_strings":["University of Michigan, Department of Biological Chemistry and Department of Chemistry https://www.nature.com/nature","University of Michigan, Department of Biological Chemistry and Department of Chemistry"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Michigan, Department of Biological Chemistry and Department of Chemistry https://www.nature.com/nature","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"University of Michigan, Department of Biological Chemistry and Department of Chemistry","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Alexander Kurochkin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alexander Kurochkin","raw_affiliation_strings":["University of Michigan, Department of Biological Chemistry and Department of Chemistry https://www.nature.com/nature","University of Michigan, Department of Biological Chemistry and Department of Chemistry"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Michigan, Department of Biological Chemistry and Department of Chemistry https://www.nature.com/nature","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"University of Michigan, Department of Biological Chemistry and Department of Chemistry","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002582823","display_name":"Grover N.B. Yip","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Grover Yip","raw_affiliation_strings":["University of Michigan, Department of Biological Chemistry and Department of Chemistry https://www.nature.com/nature","University of Michigan, Department of Biological Chemistry and Department of Chemistry"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Michigan, Department of Biological Chemistry and Department of Chemistry https://www.nature.com/nature","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"University of Michigan, Department of Biological Chemistry and Department of Chemistry","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009719847","display_name":"Eric B. Bertelsen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eric Bertelsen","raw_affiliation_strings":["University of Michigan, Department of Biological Chemistry and Department of Chemistry https://www.nature.com/nature","University of Michigan, Department of Biological Chemistry and Department of Chemistry"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Michigan, Department of Biological Chemistry and Department of Chemistry https://www.nature.com/nature","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"University of Michigan, Department of Biological Chemistry and Department of Chemistry","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009411980","display_name":"Erik R. P. Zuiderweg","orcid":"https://orcid.org/0000-0001-6766-6446","observed_orcids":["https://orcid.org/0000-0001-6766-6446"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Erik Zuiderweg","raw_affiliation_strings":["University of Michigan, Department of Biological Chemistry and Department of Chemistry https://www.nature.com/nature","University of Michigan, Department of Biological Chemistry and Department of Chemistry"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Michigan, Department of Biological Chemistry and Department of Chemistry https://www.nature.com/nature","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"University of Michigan, Department of Biological Chemistry and Department of Chemistry","institution_ids":["https://openalex.org/I27837315"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5009411980"],"corresponding_institution_ids":["https://openalex.org/I27837315"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hsp70-chaperones","display_name":"Hsp70 chaperones","score":0.9100000262260437},{"id":"https://openalex.org/keywords/allosteric-communication","display_name":"allosteric communication","score":0.9639999866485596},{"id":"https://openalex.org/keywords/nucleotide-binding-domain","display_name":"nucleotide-binding domain","score":0.9589999914169312},{"id":"https://openalex.org/keywords/substrate-binding-domain","display_name":"substrate-binding domain","score":0.8700000047683716},{"id":"https://openalex.org/keywords/thermus-thermophilus","display_name":"Thermus thermophilus","score":0.7839999794960022},{"id":"https://openalex.org/keywords/amp-pnp","display_name":"AMP-PNP","score":0.7770000100135803},{"id":"https://openalex.org/keywords/protein-conformational-change","display_name":"protein conformational change","score":0.6859999895095825},{"id":"https://openalex.org/keywords/residual-dipolar-couplings","display_name":"residual dipolar couplings","score":0.7409999966621399},{"id":"https://openalex.org/keywords/protein-folding","display_name":"protein folding","score":0.6290000081062317}],"concepts":[{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.8604918718338013},{"id":"https://openalex.org/C2780883830","wikidata":"https://www.wikidata.org/wiki/Q139470","display_name":"Thermus thermophilus","level":4,"score":0.7147338390350342},{"id":"https://openalex.org/C109964505","wikidata":"https://www.wikidata.org/wiki/Q3321436","display_name":"Allosteric enzyme","level":4,"score":0.6475420594215393},{"id":"https://openalex.org/C2775962898","wikidata":"https://www.wikidata.org/wiki/Q5073152","display_name":"Chaperone (clinical)","level":2,"score":0.6297656893730164},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5449532270431519},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.5243654251098633},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.5181483030319214},{"id":"https://openalex.org/C113027372","wikidata":"https://www.wikidata.org/wiki/Q24768350","display_name":"Cyclic nucleotide-binding domain","level":4,"score":0.49733737111091614},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.43563610315322876},{"id":"https://openalex.org/C204328495","wikidata":"https://www.wikidata.org/wiki/Q847556","display_name":"Protein folding","level":2,"score":0.43273070454597473},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4307517409324646},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.41280707716941833},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.26812660694122314},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1787596344947815},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1038/npre.2008.1978.1","doi":"https://doi.org/10.1038/npre.2008.1978.1","is_oa":true,"landing_page_url":"https://doi.org/10.1038/npre.2008.1978.1","pdf_url":"https://www.nature.com/articles/npre.2008.1978.1.pdf","source":{"id":"https://openalex.org/S48744551","display_name":"Nature Precedings","issn_l":"1756-0357","issn":["1756-0357"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Precedings","raw_type":"journal-article"},{"id":"pmh:oai:nature.com:10101/npre.2008.1978.1","doi":null,"is_oa":true,"landing_page_url":"http://precedings.nature.com/documents/1978/version/1","pdf_url":null,"source":{"id":"https://openalex.org/S48744551","display_name":"Nature Precedings","issn_l":"1756-0357","issn":["1756-0357"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Nature Precedings","raw_type":"Manuscript"}],"best_oa_location":{"id":"doi:10.1038/npre.2008.1978.1","doi":"https://doi.org/10.1038/npre.2008.1978.1","is_oa":true,"landing_page_url":"https://doi.org/10.1038/npre.2008.1978.1","pdf_url":"https://www.nature.com/articles/npre.2008.1978.1.pdf","source":{"id":"https://openalex.org/S48744551","display_name":"Nature Precedings","issn_l":"1756-0357","issn":["1756-0357"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Precedings","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320306159","display_name":"W. M. Keck Foundation","ror":"https://ror.org/000dswa46"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332460","display_name":"College of Pharmacy, University of Michigan","ror":"https://ror.org/00jmfr291"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W33309701.pdf","grobid_xml":"https://content.openalex.org/works/W33309701.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1976476593","https://openalex.org/W2109052254","https://openalex.org/W2055269320","https://openalex.org/W591955276","https://openalex.org/W2089271742","https://openalex.org/W2129194339","https://openalex.org/W1999268424","https://openalex.org/W1980505864","https://openalex.org/W1972828492","https://openalex.org/W2086312214"],"abstract_inverted_index":{"Abstract":[0],"This":[1],"work":[2],"solves":[3],"a":[4,57,65,110,194,218,259],"decades-old":[5],"dilemma":[6,170],"that":[7,71,179,234,258],"stood":[8],"in":[9,34,81,89,123,142,174,190,207],"the":[10,14,73,82,85,90,117,120,128,135,146,152,156,159,169,177,186,199,202,208,215,223,235,248,267,278,281,293,297],"way":[11],"of":[12,17,53,112,119,134,158,168,176,198,201,227,280],"understanding":[13],"allosteric":[15,121,188,268,282],"mechanism":[16],"Hsp70":[18,203,287],"(heat":[19],"shock":[20],"70":[21],"kDa)":[22],"chaperone":[23],"proteins.":[24,125],"Hsp70s":[25,50,180],"are":[26,51,92,181],"central":[27],"to":[28,39,115,263],"protein":[29],"folding,":[30],"refolding,":[31],"and":[32,45,64,84,148,164,184,211,251,272,284],"trafficking":[33],"organisms":[35],"ranging":[36],"from":[37,204],"Archae":[38],"Homo":[40],"Sapiens,":[41],"both":[42],"at":[43,46],"normal":[44],"stressed":[47],"cellular":[48],"conditions.":[49],"comprised":[52],"two":[54],"main":[55],"domains:":[56],"44":[58],"kDa":[59,67],"N-terminal":[60],"nucleotide-binding":[61],"domain":[62,69],"(NBD),":[63],"25":[66],"substrate-binding":[68],"(SBD)":[70],"harbors":[72],"substrate":[74,86,98,104],"binding":[75,79,87,96,102,237],"site.":[76],"The":[77,166],"nucleotide":[78,160,236],"site":[80,88],"NBD":[83,137,163,200,271],"SBD":[91],"allosterically":[93],"linked:":[94],"ADP":[95,149,210,252],"promotes":[97,103],"binding,":[99],"while":[100],"ATP":[101,147,220],"release.":[105],"It":[106],"has":[107],"long":[108],"been":[109],"goal":[111],"structural":[113],"biology":[114],"characterize":[116],"nature":[118,279],"coupling":[122,230,269,285],"these":[124,275],"However,":[126],"even":[127],"most":[129],"sophisticated":[130],"X-ray":[131],"crystallography":[132],"studies":[133],"isolated":[136],"could":[138],"show":[139],"no":[140],"difference":[141],"overall":[143],"conformation":[144],"between":[145,162,247,270],"state.":[150,254],"Hence":[151],"dilemma:":[153],"how":[154],"is":[155,171,217,289],"state":[157],"communicated":[161],"SBD?":[165],"solution":[167,195],"especially":[172],"interesting":[173],"light":[175],"fact":[178],"ancient":[182],"proteins,":[183],"amongst":[185],"first":[187,294],"proteins":[189],"nature.Here":[191],"we":[192,232],"report":[193],"NMR":[196,225],"study":[197],"Thermus":[205],"thermophilus,":[206],"APO,":[209],"AMP-PNP":[212,249],"states,":[213],"where":[214],"latter":[216],"non-hydrolysable":[219],"analogue.":[221],"Using":[222],"modern":[224],"methods":[226],"residual":[228],"dipolar":[229],"analysis,":[231],"discovered":[233],"cleft":[238],"opens":[239],"up":[240],"by":[241],"as":[242,244],"much":[243],"20":[245],"degrees":[246],"(closed)":[250],"(open)":[253],"We":[255],"also":[256],"discover":[257],"surface":[260],"cleft,":[261],"hypothesized":[262],"be":[264],"essential":[265],"for":[266,286,292],"SBD,":[273],"echoes":[274],"changes.":[276],"Hence,":[277],"trigger":[283],"chaperones":[288],"revealed":[290],"here":[291],"time,":[295],"solving":[296],"dilemma.":[298]},"counts_by_year":[{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-01T08:54:57.152042","created_date":"2016-06-24T00:00:00"}