{"id":"https://openalex.org/W33254170","doi":null,"title":"Comparative analysis of class I, II and III epitope-detecting CD34 monoclonal antibodies by quantitative flow cytometry.","display_name":"Comparative analysis of class I, II and III epitope-detecting CD34 monoclonal antibodies by quantitative flow cytometry.","publication_year":1998,"publication_date":"1998-12-01","ids":{"openalex":"https://openalex.org/W33254170","mag":"33254170","pmid":"https://pubmed.ncbi.nlm.nih.gov/9924925"},"language":"en","primary_location":{"id":"pmid:9924925","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9924925","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hematology and cell therapy","raw_type":"Comparative Study"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045576636","display_name":"Hanna Sovalat","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210135664","display_name":"H\u00f4pital du Hasenrain","ror":"https://ror.org/04d45ra64","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210117936","https://openalex.org/I4210135664"]},{"id":"https://openalex.org/I4210139102","display_name":"Institut de Recherche en H\u00e9matologie et Transplantation","ror":"https://ror.org/039ztcx57","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210139102"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"H Sovalat","raw_affiliation_strings":["Institut de Recherche en H\u00e9matologie et Transfusion, H\u00f4pital du Hasenrain, Mulhouse, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut de Recherche en H\u00e9matologie et Transfusion, H\u00f4pital du Hasenrain, Mulhouse, France","institution_ids":["https://openalex.org/I4210135664","https://openalex.org/I4210139102"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015380178","display_name":"Evelyne Racadot","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"E Racadot","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072243285","display_name":"Philippe R.G. Henon","orcid":"https://orcid.org/0000-0002-8798-5994","observed_orcids":["https://orcid.org/0000-0002-8798-5994"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P H\u00e9non","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011839226","display_name":"PETER C. FUCHS","orcid":"https://orcid.org/0000-0003-3933-7674","observed_orcids":["https://orcid.org/0000-0003-3933-7674"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P Fuchs","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076393298","display_name":"H. Lewandowski","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Lewandowski","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5029225323","display_name":"Maryse Billot","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Billot","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.2125,"has_fulltext":false,"cited_by_count":9,"citation_normalized_percentile":{"value":0.48605904,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"40","issue":"6","first_page":"259","last_page":"68"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.46630001068115234,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.46630001068115234,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.24779999256134033,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11346","display_name":"Mast cells and histamine","score":0.0284000001847744,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cd34","display_name":"CD34","score":0.8709999918937683},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.9610000252723694},{"id":"https://openalex.org/keywords/antigen-density","display_name":"antigen density","score":0.7009999752044678},{"id":"https://openalex.org/keywords/hematopoietic-stem-cells","display_name":"hematopoietic stem cells","score":0.7540000081062317},{"id":"https://openalex.org/keywords/bone-marrow","display_name":"bone marrow","score":0.8529999852180481}],"concepts":[{"id":"https://openalex.org/C10205521","wikidata":"https://www.wikidata.org/wiki/Q4035578","display_name":"CD34","level":3,"score":0.7304348349571228},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.7084547281265259},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6726301908493042},{"id":"https://openalex.org/C159912055","wikidata":"https://www.wikidata.org/wiki/Q14885119","display_name":"CD38","level":4,"score":0.6665709614753723},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.6595160961151123},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.5459507703781128},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.529496967792511},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.49070656299591064},{"id":"https://openalex.org/C2778236600","wikidata":"https://www.wikidata.org/wiki/Q592324","display_name":"Immunofluorescence","level":3,"score":0.41965654492378235},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.4127289354801178},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3732226490974426},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.330710768699646},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.18193429708480835},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.06478267908096313}],"mesh":[{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000939","descriptor_name":"Epitopes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D000943","descriptor_name":"Antigens, Differentiation","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001854","descriptor_name":"Bone Marrow Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001854","descriptor_name":"Bone Marrow Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002452","descriptor_name":"Cell Count","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007963","descriptor_name":"Leukocytes, Mononuclear","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D007963","descriptor_name":"Leukocytes, Mononuclear","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008562","descriptor_name":"Membrane Glycoproteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009244","descriptor_name":"NAD+ Nucleosidase","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D013234","descriptor_name":"Stem Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D013234","descriptor_name":"Stem Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015703","descriptor_name":"Antigens, CD","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016131","descriptor_name":"Lymphocyte Subsets","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D016131","descriptor_name":"Lymphocyte Subsets","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018952","descriptor_name":"Antigens, CD34","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D019084","descriptor_name":"Fluorescent Antibody Technique, Indirect","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D036541","descriptor_name":"ADP-ribosyl Cyclase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051997","descriptor_name":"ADP-ribosyl Cyclase 1","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:9924925","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9924925","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hematology and cell therapy","raw_type":"Comparative Study"},{"id":"mag:33254170","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9924925","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":8,"referenced_works":["https://openalex.org/W2097746043","https://openalex.org/W2275619513","https://openalex.org/W2077571864","https://openalex.org/W2055566690","https://openalex.org/W1509975354","https://openalex.org/W2008905653","https://openalex.org/W2161552346","https://openalex.org/W2145486738"],"related_works":["https://openalex.org/W1978233800","https://openalex.org/W2004863725","https://openalex.org/W196303326","https://openalex.org/W2045894831","https://openalex.org/W2420389054","https://openalex.org/W2509876506","https://openalex.org/W2124898580","https://openalex.org/W2188997971","https://openalex.org/W2034909662","https://openalex.org/W7098510585"],"abstract_inverted_index":{"UNLABELLED:":[0],"The":[1,142,200],"aim":[2],"of":[3,27,56,62,78,99,112,129,144,158,237],"this":[4],"study":[5,77],"was":[6,115,133,139,148,152,203],"to":[7,15,96],"compare":[8],"different":[9,17],"CD34":[10,37,63,84,118,130,162,195,211],"monoclonal":[11],"antibodies":[12],"(MAbs)":[13],"belonging":[14],"three":[16,28],"classes:":[18],"MY10":[19,146,181,222],"class":[20,23,31,41,171,182,192,197,226,245],"I,":[21],"QBend10":[22,191],"II,":[24],"a":[25,70,122],"mixture":[26],"selected":[29],"MAbs":[30,215,230,247],"I":[32,183],"and":[33,39,85,103,136,151,163,194,228],"II":[34,193,227],"designated":[35],"as":[36],"Pool,":[38,119],"8G12":[40,88,170,214],"III.":[42],"Bone":[43],"marrow":[44],"(BM)":[45],"samples":[46],"from":[47],"13":[48],"healthy":[49],"donors":[50],"were":[51],"analyzed":[52],"for:":[53],"1)":[54],"percentage":[55,98,111,128],"CD34+":[57,79,101,113,159,238,241],"cells,":[58],"2)":[59],"quantitative":[60,71],"expression":[61,143],"epitopes":[64],"(antigen's":[65],"density":[66],"-":[67],"AgD)":[68],"using":[69],"indirect":[72],"immunofluorescence":[73],"(QIFI)":[74],"test,":[75],"3)":[76],"cell":[80,242],"subsets":[81,243],"defined":[82],"by":[83,209],"CD38":[86,164],"coexpression.":[87],"MAb":[89,173],"showed":[90],"the":[91,97,127,137,145,149,155,206],"highest":[92],"reactivity":[93],"with":[94,117,121,177,180,186,205,217],"regard":[95],"detected":[100,116,153,174,204],"cells":[102,114,132,176],"AgD":[104,138],"on":[105,154],"these":[106],"cells.":[107,160],"A":[108],"nearly":[109],"identical":[110],"but":[120,185,216],"lower":[123],"AgD.":[124],"With":[125],"QBend10,":[126,210],"expressing":[131],"insignificantly":[134],"decreased":[135],"slightly":[140],"lower.":[141],"epitope":[147],"lowest":[150,156],"number":[157],"Concerning":[161],"coexpressing":[165],"subset,":[166],"we":[167],"observed":[168],"that":[169],"III":[172,229,246],"CD34loCD38dim":[175],"comparable":[178],"efficiency":[179,208],"MAb,":[184],"significantly":[187,218],"higher":[188,219],"level":[189],"than":[190,221],"Pool":[196,212],"I+II":[198],"MAbs.":[199],"CD34hiCD38dim":[201],"subset":[202],"same":[207],"or":[213],"frequency":[220],"MAb.":[223],"IN":[224],"CONCLUSION:":[225],"appear":[231],"preferable":[232],"for":[233,240],"flow":[234],"cytometric":[235],"quantification":[236],"cells;":[239],"determination":[244],"should":[248],"be":[249],"suitable.":[250]},"counts_by_year":[{"year":2018,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}