{"id":"https://openalex.org/W332226709","doi":null,"title":"The effect of hydrocortisone on the incorporation of tritiated thymidine by human blood lymphocytes cultured with phytohaemagglutinin and pokeweed mitogen.","display_name":"The effect of hydrocortisone on the incorporation of tritiated thymidine by human blood lymphocytes cultured with phytohaemagglutinin and pokeweed mitogen.","publication_year":1973,"publication_date":"1973-10-01","ids":{"openalex":"https://openalex.org/W332226709","mag":"332226709","pmid":"https://pubmed.ncbi.nlm.nih.gov/4762017","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1553886"},"language":"en","primary_location":{"id":"pmh:oai:pubmedcentral.nih.gov:1553886","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1553886","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clin Exp Immunol","raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1553886","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074724229","display_name":"Dorothy H. Heilman","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D H Heilman","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016564820","display_name":"Margaret R. Gambrill","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M R Gambrill","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5006228644","display_name":"J. P. Leichner","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J P Leichner","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.0299,"has_fulltext":false,"cited_by_count":65,"citation_normalized_percentile":{"value":0.85157724,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"15","issue":"2","first_page":"203","last_page":"12"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T14072","display_name":"Immunotoxicology and immune responses","score":0.3176000118255615,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T14072","display_name":"Immunotoxicology and immune responses","score":0.3176000118255615,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.2653999924659729,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10529","display_name":"Stress Responses and Cortisol","score":0.12219999730587006,"subfield":{"id":"https://openalex.org/subfields/2802","display_name":"Behavioral Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cortisol","display_name":"cortisol","score":1.0},{"id":"https://openalex.org/keywords/corticosteroids","display_name":"corticosteroids","score":0.7110000252723694},{"id":"https://openalex.org/keywords/lymphocyte-transformation","display_name":"lymphocyte transformation","score":0.8240000009536743},{"id":"https://openalex.org/keywords/phytohemagglutinin","display_name":"phytohemagglutinin","score":0.9679999947547913},{"id":"https://openalex.org/keywords/pokeweed-mitogen","display_name":"pokeweed mitogen","score":1.0},{"id":"https://openalex.org/keywords/thymidine-incorporation","display_name":"thymidine incorporation","score":0.890999972820282},{"id":"https://openalex.org/keywords/b-cells","display_name":"B cells","score":0.8949999809265137},{"id":"https://openalex.org/keywords/lymphocyte-proliferation","display_name":"lymphocyte proliferation","score":0.36000001430511475}],"concepts":[{"id":"https://openalex.org/C2781005381","wikidata":"https://www.wikidata.org/wiki/Q7208633","display_name":"Pokeweed mitogen","level":4,"score":0.9358469247817993},{"id":"https://openalex.org/C2777108182","wikidata":"https://www.wikidata.org/wiki/Q422464","display_name":"Thymidine","level":3,"score":0.8242589235305786},{"id":"https://openalex.org/C2780050583","wikidata":"https://www.wikidata.org/wiki/Q2702796","display_name":"Phytohaemagglutinin","level":3,"score":0.8223477602005005},{"id":"https://openalex.org/C2778709874","wikidata":"https://www.wikidata.org/wiki/Q190875","display_name":"Hydrocortisone","level":2,"score":0.707482099533081},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.5994807481765747},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.5885438919067383},{"id":"https://openalex.org/C24998067","wikidata":"https://www.wikidata.org/wiki/Q4114622","display_name":"Stimulation","level":2,"score":0.5861765742301941},{"id":"https://openalex.org/C71315377","wikidata":"https://www.wikidata.org/wiki/Q11364","display_name":"Hormone","level":2,"score":0.4907478094100952},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4857404828071594},{"id":"https://openalex.org/C25642318","wikidata":"https://www.wikidata.org/wiki/Q1992532","display_name":"Incubation","level":2,"score":0.4337863326072693},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.42287909984588623},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.2482338547706604},{"id":"https://openalex.org/C19869320","wikidata":"https://www.wikidata.org/wiki/Q419277","display_name":"Concanavalin A","level":3,"score":0.22102820873260498},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2023608386516571},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.19790923595428467},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006854","descriptor_name":"Hydrocortisone","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D008213","descriptor_name":"Lymphocyte Activation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D008214","descriptor_name":"Lymphocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008264","descriptor_name":"Macrophages","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D008934","descriptor_name":"Mitogens","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D009040","descriptor_name":"Motion Pictures","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013936","descriptor_name":"Thymidine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014316","descriptor_name":"Tritium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D037102","descriptor_name":"Lectins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true}],"locations_count":3,"locations":[{"id":"pmh:oai:pubmedcentral.nih.gov:1553886","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1553886","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clin Exp Immunol","raw_type":"Text"},{"id":"pmid:4762017","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4762017","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical and experimental immunology","raw_type":"Journal Article"},{"id":"mag:332226709","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4762017","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:1553886","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1553886","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clin Exp Immunol","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.5683000087738037}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W118652452","https://openalex.org/W321217933","https://openalex.org/W1576254167","https://openalex.org/W1588053635","https://openalex.org/W1596318490","https://openalex.org/W1600051139","https://openalex.org/W1601513347","https://openalex.org/W1837470427","https://openalex.org/W1977480729","https://openalex.org/W2006684546","https://openalex.org/W2028258977","https://openalex.org/W2043145077","https://openalex.org/W2043326980","https://openalex.org/W2050543828","https://openalex.org/W2054657547","https://openalex.org/W2093117467","https://openalex.org/W2103179215","https://openalex.org/W2413493946","https://openalex.org/W4313376843"],"related_works":["https://openalex.org/W2027690843","https://openalex.org/W2413493946","https://openalex.org/W369877331","https://openalex.org/W1596455821","https://openalex.org/W4313310675","https://openalex.org/W2047901099","https://openalex.org/W1979943156","https://openalex.org/W2021147242","https://openalex.org/W351039942","https://openalex.org/W1918607841"],"abstract_inverted_index":{"Methods":[0],"based":[1],"on":[2,12,17,131,141],"[(3)H]thymidine":[3,48,99,194,220],"incorporation":[4,49,100],"and":[5,26,79,109,114,128,147,178,216,231],"morphology":[6],"were":[7,151],"used":[8],"for":[9,76],"further":[10],"studies":[11],"the":[13,18,90,123,142,168,229,236,247],"effect":[14],"of":[15,20,37,46,62,87,92,112,145,165,170,176,181,184,193,207,210,218,238,249],"hydrocortisone":[16,63,166,250],"transformation":[19,237],"human":[21],"lymphocytes":[22,240],"by":[23,59,107,241],"phytohaemagglutinin":[24],"(PHA)":[25],"pokeweed":[27],"mitogen":[28],"(PWM).":[29],"Result":[30],"obtained":[31,227],"with":[32,43,228],"both":[33,174],"methods":[34,233],"showed":[35,134],"inhibition":[36],"PHA":[38],"stimulation":[39,118],"in":[40,53,64,72,94,103,153,158,173,186,196,213,251],"cultures":[41,56,65,74,84,106,177,198],"preincubated":[42],"0\u00b701\u201310":[44],"\u03bcg/ml":[45,61,86,111,116],"hydrocortisone.":[47],"due":[50,203,221],"to":[51,122,201,204,222,246],"PWM":[52,105,125,137,171,242],"vertical":[54,83,188,197],"tube":[55,189],"was":[57,101,199],"depressed":[58,89],"1\u00b70":[60,110],"harvested":[66],"at":[67],"48":[68],"hr,":[69],"but":[70],"not":[71],"similar":[73],"incubated":[75],"3,":[77],"4":[78],"5":[80,149],"days.":[81],"In":[82],"10":[85,115],"hormone":[88,212],"level":[91],"uptake":[93,195],"most":[95],"experiments.":[96],"By":[97],"contrast":[98],"enhanced":[102,208],"horizontal":[104],"0\u00b71":[108],"hydrocortisone,":[113],"caused":[117,179],"or":[119],"a":[120,205],"return":[121],"normal":[124],"level.":[126],"Microscopic":[127],"time-lapse":[129],"observations":[130],"living":[132],"cells":[133],"that":[135,235],"typical":[136],"blasts":[138,172,185],"first":[139],"appeared":[140],"third":[143],"day":[144],"incubation":[146],"after":[148],"days":[150],"numerous":[152],"corticosteroid-treated":[154],"as":[155,157],"well":[156],"untreated":[159],"cultures.":[160,190],"Ten":[161],"micrograms":[162],"per":[163],"millilitre":[164],"increased":[167,223],"fragility":[169],"kinds":[175],"degeneration":[180],"variable":[182],"numbers":[183],"5-day-old":[187],"The":[191],"depression":[192],"thought":[200],"be":[202],"combination":[206],"toxicity":[209],"corticosteroid":[211],"deep":[214],"cultures,":[215],"loss":[217],"incorporated":[219],"cellular":[224],"fragility.":[225],"Results":[226],"isotopic":[230],"morphologic":[232],"indicated":[234],"B":[239],"is":[243],"relatively":[244],"resistant":[245],"action":[248],"vitro.":[252]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}