{"id":"https://openalex.org/W323642754","doi":null,"title":"Kinetics and mechanism of the interaction of the C1 domain of protein kinase C with lipid membranes","display_name":"Kinetics and mechanism of the interaction of the C1 domain of protein kinase C with lipid membranes","publication_year":2007,"publication_date":"2007-01-01","ids":{"openalex":"https://openalex.org/W323642754","mag":"323642754"},"language":"en","primary_location":{"id":"pmh:oai:escholarship.org/ark:/13030/qt1vw5x6bm","doi":null,"is_oa":true,"landing_page_url":"https://escholarship.org/uc/item/1vw5x6bm","pdf_url":"https://escholarship.org/uc/item/1vw5x6bm","source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},"volume":null,"type":"dissertation","indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://escholarship.org/uc/item/1vw5x6bm","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051057068","display_name":"Daniel Robert Dries","orcid":"https://orcid.org/0000-0002-0530-5212","observed_orcids":["https://orcid.org/0000-0002-0530-5212"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Daniel R. Dries","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-0530-5212","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5051057068"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.9473000168800354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.9473000168800354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.04910000041127205,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11308","display_name":"Sphingolipid Metabolism and Signaling","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-kinase-c","display_name":"protein kinase C","score":0.9950000047683716},{"id":"https://openalex.org/keywords/c1-domain","display_name":"C1 domain","score":0.996999979019165},{"id":"https://openalex.org/keywords/membrane-binding","display_name":"membrane binding","score":0.5870000123977661},{"id":"https://openalex.org/keywords/diacylglycerol","display_name":"diacylglycerol","score":0.9800000190734863},{"id":"https://openalex.org/keywords/phorbol-ester","display_name":"phorbol ester","score":0.8740000128746033},{"id":"https://openalex.org/keywords/binding-kinetics","display_name":"binding kinetics","score":0.7730000019073486},{"id":"https://openalex.org/keywords/hydrophobic-interactions","display_name":"hydrophobic interactions","score":0.6769999861717224},{"id":"https://openalex.org/keywords/binding-pocket","display_name":"binding pocket","score":0.7889999747276306},{"id":"https://openalex.org/keywords/golgi-localization","display_name":"Golgi localization","score":0.8889999985694885}],"concepts":[{"id":"https://openalex.org/C195794163","wikidata":"https://www.wikidata.org/wiki/Q420877","display_name":"Protein kinase C","level":3,"score":0.6479120254516602},{"id":"https://openalex.org/C143840085","wikidata":"https://www.wikidata.org/wiki/Q5008102","display_name":"C2 domain","level":3,"score":0.6344068646430969},{"id":"https://openalex.org/C36020004","wikidata":"https://www.wikidata.org/wiki/Q106951674","display_name":"Diacylglycerol kinase","level":4,"score":0.6072720289230347},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.5778068900108337},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5014574527740479},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4286208152770996},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.42742007970809937},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4144664406776428},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.3271269202232361},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3180239200592041}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:escholarship.org/ark:/13030/qt1vw5x6bm","doi":null,"is_oa":true,"landing_page_url":"https://escholarship.org/uc/item/1vw5x6bm","pdf_url":"https://escholarship.org/uc/item/1vw5x6bm","source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},{"id":"mag:323642754","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/content/qt1vw5x6bm/qt1vw5x6bm.pdf?t=lq64r8","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:escholarship.org/ark:/13030/qt1vw5x6bm","doi":null,"is_oa":true,"landing_page_url":"https://escholarship.org/uc/item/1vw5x6bm","pdf_url":"https://escholarship.org/uc/item/1vw5x6bm","source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332603","display_name":"University of California, San Diego","ror":"https://ror.org/0168r3w48"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W323642754.pdf","grobid_xml":"https://content.openalex.org/works/W323642754.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2765315469","https://openalex.org/W2188476874","https://openalex.org/W2032959153","https://openalex.org/W236817708","https://openalex.org/W7067395171","https://openalex.org/W4233767967","https://openalex.org/W7095641381","https://openalex.org/W3133007942","https://openalex.org/W2101773993","https://openalex.org/W2066855988"],"abstract_inverted_index":{"Protein":[0],"kinase":[1],"C":[2],"(PKC)":[3],"is":[4,74,176],"a":[5,71,130,177,279,294,299,305,311],"critical":[6],"component":[7],"of":[8,34,40,65,104,123,188,227,234,241,260,265,281,301,326,329,343],"many":[9],"signaling":[10],"pathways.":[11],"As":[12],"such,":[13],"PKC":[14,41,66,243,248],"regulates":[15,31,163,224],"such":[16],"diverse":[17],"phenomena":[18],"as":[19],"memory":[20],"and":[21,26,36,89,98,117,144,192,231,245,336],"learning,":[22],"immune":[23],"system":[24],"responses,":[25],"cancer.":[27],"Binding":[28],"to":[29,43,47,80,142,182,214,278],"membranes":[30,44,143,335],"the":[32,37,48,57,62,82,86,102,105,114,121,134,138,147,164,186,189,194,201,211,225,228,232,238,261,269,285,323,327,330],"activity":[33],"PKC,":[35],"C1":[38,63,87,106,115,139,165,254,263,270,307,331],"domain":[39,64,88,107,116,140,213,264,271,290,332],"binds":[42,141],"in":[45,319],"response":[46],"lipid":[49,90,334],"second":[50],"messenger,":[51],"diacylglycerol":[52],"(DG).":[53],"This":[54,173],"dissertation":[55,321],"describes":[56,133],"molecular":[58,324],"nature":[59,325],"by":[60,136],"which":[61,137,175],"interacts":[67],"with":[68,76,108,333],"membranes.":[69,216],"First,":[70],"fluorescent":[72,78],"reporter":[73],"used":[75],"stopped-flow":[77],"spectroscopy":[79],"analyze":[81],"binding":[83,286],"kinetics":[84],"between":[85,113],"vesicles.":[91],"Kinetic":[92],"data":[93,126,317],"reveal":[94],"that":[95,132,162,220,268],"both":[96],"specific":[97],"non-specific":[99],"interactions":[100],"guide":[101],"association":[103],"membranes,":[109],"whereas":[110],"hydrophobic":[111],"contacts":[112],"its":[118],"ligand":[119],"determine":[120],"rate":[122],"dissociation.":[124],"The":[125,316],"provide":[127],"evidence":[128],"for":[129,168,196,203,256,298],"model":[131],"mechanism":[135],"explain":[145],"how":[146],"DG-mimicking,":[148],"tumor-promoting":[149],"phorbol":[150],"esters":[151],"(i.e.,":[152],"PMA)":[153],"exert":[154],"their":[155,253],"potency.":[156],"Second,":[157],"an":[158],"evolutionarily-":[159],"conserved":[160],"residue":[161,236],"domain's":[166],"affinity":[167,195,202,297],"DG":[169,197,274],"has":[170],"been":[171],"identified.":[172],"residue,":[174],"conservative":[178],"change":[179],"from":[180],"tryptophan":[181,205],"tyrosine,":[183],"lies":[184],"at":[185,206],"apex":[187],"ligand-binding":[190,229],"pocket":[191],"lowers":[193],"30-fold":[198],"without":[199],"affecting":[200],"PMA;":[204],"this":[207,221,235,289,320,340],"position":[208],"also":[209],"localizes":[210],"isolated":[212],"internal":[215],"Molecular":[217],"modeling":[218,259],"suggests":[219],"amino":[222],"acid":[223],"width":[226],"pocket,":[230],"identity":[233],"explains":[237],"Golgi":[239],"localization":[240],"novel":[242,247],"isoforms":[244,249],"why":[246],"rely":[250],"solely":[251],"on":[252],"domains":[255,345],"activation.":[257],"Finally,":[258],"atypical":[262],"PKCz":[266],"reveals":[267],"cannot":[272],"bind":[273],"or":[275],"PMA":[276],"due":[277],"cluster":[280],"basic":[282],"residues":[283],"occluding":[284],"pocket.":[287],"Moreover,":[288],"does":[291,304],"not":[292],"have":[293],"higher":[295],"intrinsic":[296],"variety":[300],"phospholipids":[302],"than":[303],"typical":[306],"domain,":[308],"despite":[309],"having":[310],"more":[312],"positive":[313],"electrostatic":[314],"potential.":[315],"presented":[318],"redefine":[322],"interaction":[328],"offer":[337],"insights":[338],"into":[339],"unique":[341],"class":[342],"lipid-targeting":[344]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}