{"id":"https://openalex.org/W322651592","doi":null,"title":"Production, Purification and Characterization of Recombinant Viral Proteins","display_name":"Production, Purification and Characterization of Recombinant Viral Proteins","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W322651592","mag":"322651592"},"language":"en","primary_location":{"id":"mag:322651592","doi":null,"is_oa":false,"landing_page_url":"http://ethesis.nitrkl.ac.in/4452/1/Full_PhD_Thesis-508CH802.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5062072057","display_name":"Nagesh Kumar Tripathi","orcid":"https://orcid.org/0000-0003-3533-3262","observed_orcids":["https://orcid.org/0000-0003-3533-3262"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Nagesh K. Tripathi","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-3533-3262","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5062072057"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.005900000222027302,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.0024999999441206455,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dengue-virus","display_name":"dengue virus","score":0.921999990940094},{"id":"https://openalex.org/keywords/japanese-encephalitis-virus","display_name":"Japanese encephalitis virus","score":0.996999979019165},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8690000176429749},{"id":"https://openalex.org/keywords/inclusion-bodies","display_name":"inclusion bodies","score":0.7620000243186951},{"id":"https://openalex.org/keywords/protein-refolding","display_name":"protein refolding","score":0.6359999775886536},{"id":"https://openalex.org/keywords/affinity-chromatography","display_name":"affinity chromatography","score":0.6740000247955322},{"id":"https://openalex.org/keywords/vaccine-candidate","display_name":"vaccine candidate","score":0.5490000247955322},{"id":"https://openalex.org/keywords/batch-fermentation","display_name":"batch fermentation","score":0.5709999799728394},{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.9980000257492065},{"id":"https://openalex.org/keywords/culture-medium-optimization","display_name":"culture medium optimization","score":0.8569999933242798},{"id":"https://openalex.org/keywords/ion-exchange-chromatography","display_name":"ion-exchange chromatography","score":0.7099999785423279},{"id":"https://openalex.org/keywords/antibody-detection","display_name":"antibody detection","score":0.3919999897480011},{"id":"https://openalex.org/keywords/serological-diagnosis","display_name":"serological diagnosis","score":0.47200000286102295},{"id":"https://openalex.org/keywords/mosquito-borne-viral-infection","display_name":"mosquito-borne viral disease","score":0.49000000953674316}],"concepts":[{"id":"https://openalex.org/C533803919","wikidata":"https://www.wikidata.org/wiki/Q30953","display_name":"Dengue fever","level":2,"score":0.7106396555900574},{"id":"https://openalex.org/C2779635636","wikidata":"https://www.wikidata.org/wiki/Q476209","display_name":"Dengue virus","level":3,"score":0.6170244216918945},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5471441149711609},{"id":"https://openalex.org/C2779141303","wikidata":"https://www.wikidata.org/wiki/Q738292","display_name":"Japanese encephalitis","level":4,"score":0.5470484495162964},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5277807116508484},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5074612498283386},{"id":"https://openalex.org/C2779308166","wikidata":"https://www.wikidata.org/wiki/Q18388297","display_name":"Dengue vaccine","level":4,"score":0.5060808062553406},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.457576721906662},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.2959672212600708},{"id":"https://openalex.org/C83455156","wikidata":"https://www.wikidata.org/wiki/Q199615","display_name":"Encephalitis","level":3,"score":0.2549797296524048},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.15612900257110596},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:322651592","doi":null,"is_oa":false,"landing_page_url":"http://ethesis.nitrkl.ac.in/4452/1/Full_PhD_Thesis-508CH802.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":252,"referenced_works":["https://openalex.org/W4137992","https://openalex.org/W37702736","https://openalex.org/W113121632","https://openalex.org/W118126351","https://openalex.org/W158830970","https://openalex.org/W183210926","https://openalex.org/W188591322","https://openalex.org/W565180991","https://openalex.org/W1483154637","https://openalex.org/W1516116810","https://openalex.org/W1523829503","https://openalex.org/W1536161194","https://openalex.org/W1549531973","https://openalex.org/W1562028374","https://openalex.org/W1567194265","https://openalex.org/W1574082422","https://openalex.org/W1590081705","https://openalex.org/W1599389506","https://openalex.org/W1612962545","https://openalex.org/W1675705467","https://openalex.org/W1821174360","https://openalex.org/W1867765096","https://openalex.org/W1873199501","https://openalex.org/W1881586736","https://openalex.org/W1897163834","https://openalex.org/W1909090758","https://openalex.org/W1910318255","https://openalex.org/W1934988108","https://openalex.org/W1965111937","https://openalex.org/W1966264195","https://openalex.org/W1968157175","https://openalex.org/W1968473374","https://openalex.org/W1968838674","https://openalex.org/W1970214436","https://openalex.org/W1970455211","https://openalex.org/W1972444392","https://openalex.org/W1972486625","https://openalex.org/W1974699496","https://openalex.org/W1974840555","https://openalex.org/W1975504808","https://openalex.org/W1977501471","https://openalex.org/W1978113142","https://openalex.org/W1978974196","https://openalex.org/W1979312516","https://openalex.org/W1979633027","https://openalex.org/W1980037410","https://openalex.org/W1980511810","https://openalex.org/W1980825106","https://openalex.org/W1981794351","https://openalex.org/W1981895261","https://openalex.org/W1982219875","https://openalex.org/W1984012771","https://openalex.org/W1984119456","https://openalex.org/W1985641916","https://openalex.org/W1986082370","https://openalex.org/W1986531033","https://openalex.org/W1987228160","https://openalex.org/W1988209979","https://openalex.org/W1988527492","https://openalex.org/W1990170781","https://openalex.org/W1990413588","https://openalex.org/W1992394885","https://openalex.org/W1994321094","https://openalex.org/W1995611933","https://openalex.org/W1995940305","https://openalex.org/W1997220850","https://openalex.org/W1998908967","https://openalex.org/W1999531660","https://openalex.org/W1999676950","https://openalex.org/W1999756366","https://openalex.org/W2001245409","https://openalex.org/W2002638462","https://openalex.org/W2004044328","https://openalex.org/W2004676082","https://openalex.org/W2005906788","https://openalex.org/W2007310953","https://openalex.org/W2009760265","https://openalex.org/W2009953715","https://openalex.org/W2011206425","https://openalex.org/W2015919714","https://openalex.org/W2016129617","https://openalex.org/W2016159719","https://openalex.org/W2016317869","https://openalex.org/W2017081541","https://openalex.org/W2017207494","https://openalex.org/W2017329638","https://openalex.org/W2018544077","https://openalex.org/W2018587074","https://openalex.org/W2021733409","https://openalex.org/W2023147536","https://openalex.org/W2024244058","https://openalex.org/W2024692615","https://openalex.org/W2025121851","https://openalex.org/W2025489393","https://openalex.org/W2025621680","https://openalex.org/W2027252177","https://openalex.org/W2027765835","https://openalex.org/W2030949148","https://openalex.org/W2031336579","https://openalex.org/W2033054784","https://openalex.org/W2033134248","https://openalex.org/W2033666909","https://openalex.org/W2035317892","https://openalex.org/W2036562152","https://openalex.org/W2036954988","https://openalex.org/W2038055214","https://openalex.org/W2038433611","https://openalex.org/W2039186581","https://openalex.org/W2042479028","https://openalex.org/W2043352558","https://openalex.org/W2044671049","https://openalex.org/W2045151225","https://openalex.org/W2046787808","https://openalex.org/W2047045134","https://openalex.org/W2048441281","https://openalex.org/W2049361387","https://openalex.org/W2049424635","https://openalex.org/W2050512136","https://openalex.org/W2050699175","https://openalex.org/W2052821867","https://openalex.org/W2053448984","https://openalex.org/W2055059689","https://openalex.org/W2056302050","https://openalex.org/W2057550814","https://openalex.org/W2057829396","https://openalex.org/W2058017947","https://openalex.org/W2059197256","https://openalex.org/W2059416305","https://openalex.org/W2059955015","https://openalex.org/W2060140364","https://openalex.org/W2062190635","https://openalex.org/W2064530135","https://openalex.org/W2065952556","https://openalex.org/W2067423878","https://openalex.org/W2067528600","https://openalex.org/W2067685428","https://openalex.org/W2068825716","https://openalex.org/W2070658428","https://openalex.org/W2072592700","https://openalex.org/W2072897393","https://openalex.org/W2073665342","https://openalex.org/W2073983719","https://openalex.org/W2074393517","https://openalex.org/W2074468568","https://openalex.org/W2076145201","https://openalex.org/W2076294121","https://openalex.org/W2076679556","https://openalex.org/W2077207144","https://openalex.org/W2078375321","https://openalex.org/W2078998316","https://openalex.org/W2079394172","https://openalex.org/W2080259596","https://openalex.org/W2081153769","https://openalex.org/W2081182500","https://openalex.org/W2081529090","https://openalex.org/W2085792563","https://openalex.org/W2086652584","https://openalex.org/W2088158522","https://openalex.org/W2088245061","https://openalex.org/W2088820685","https://openalex.org/W2089576620","https://openalex.org/W2090422998","https://openalex.org/W2093681352","https://openalex.org/W2093777251","https://openalex.org/W2093844440","https://openalex.org/W2094521812","https://openalex.org/W2094943792","https://openalex.org/W2095087335","https://openalex.org/W2096188784","https://openalex.org/W2097088780","https://openalex.org/W2097875868","https://openalex.org/W2099094631","https://openalex.org/W2100837269","https://openalex.org/W2100891069","https://openalex.org/W2103578073","https://openalex.org/W2104391647","https://openalex.org/W2105152313","https://openalex.org/W2105544490","https://openalex.org/W2105886585","https://openalex.org/W2106667383","https://openalex.org/W2107860691","https://openalex.org/W2108240258","https://openalex.org/W2110495161","https://openalex.org/W2111101948","https://openalex.org/W2111260183","https://openalex.org/W2111368649","https://openalex.org/W2111986404","https://openalex.org/W2112535453","https://openalex.org/W2113039776","https://openalex.org/W2114354917","https://openalex.org/W2114582288","https://openalex.org/W2115828237","https://openalex.org/W2116743864","https://openalex.org/W2116904877","https://openalex.org/W2117072353","https://openalex.org/W2118127502","https://openalex.org/W2120354550","https://openalex.org/W2123205277","https://openalex.org/W2124937802","https://openalex.org/W2128631050","https://openalex.org/W2131112023","https://openalex.org/W2131390184","https://openalex.org/W2131522827","https://openalex.org/W2132721526","https://openalex.org/W2133312047","https://openalex.org/W2134857592","https://openalex.org/W2137995709","https://openalex.org/W2138816428","https://openalex.org/W2140568524","https://openalex.org/W2141075182","https://openalex.org/W2141380048","https://openalex.org/W2144088993","https://openalex.org/W2147560666","https://openalex.org/W2147763877","https://openalex.org/W2147967486","https://openalex.org/W2150985653","https://openalex.org/W2151265414","https://openalex.org/W2153028888","https://openalex.org/W2153280361","https://openalex.org/W2153384932","https://openalex.org/W2154600204","https://openalex.org/W2155371520","https://openalex.org/W2158515300","https://openalex.org/W2159081448","https://openalex.org/W2160475351","https://openalex.org/W2160888384","https://openalex.org/W2161070934","https://openalex.org/W2163143680","https://openalex.org/W2163412375","https://openalex.org/W2164893710","https://openalex.org/W2165014988","https://openalex.org/W2165961841","https://openalex.org/W2166371656","https://openalex.org/W2166561873","https://openalex.org/W2166931131","https://openalex.org/W2167712022","https://openalex.org/W2167780795","https://openalex.org/W2169205227","https://openalex.org/W2170703458","https://openalex.org/W2171377206","https://openalex.org/W2189481352","https://openalex.org/W2192326515","https://openalex.org/W2242254310","https://openalex.org/W2319103644","https://openalex.org/W2415437671","https://openalex.org/W2417941326","https://openalex.org/W2470014202","https://openalex.org/W2566026207","https://openalex.org/W2782303467","https://openalex.org/W2800284038","https://openalex.org/W3140551954","https://openalex.org/W6922348476"],"related_works":["https://openalex.org/W2067528600","https://openalex.org/W2151949677","https://openalex.org/W2039604885","https://openalex.org/W2027765835","https://openalex.org/W2267525644","https://openalex.org/W2030224342","https://openalex.org/W2607362261","https://openalex.org/W2038433611","https://openalex.org/W2030100681","https://openalex.org/W4405260682"],"abstract_inverted_index":{"Dengue":[0,167,474],"fever,":[1],"a":[2,8,15,57,334],"mosquito-borne":[3],"viral":[4,497],"disease":[5],"has":[6],"become":[7],"major":[9],"worldwide":[10],"public":[11],"health":[12],"problem":[13],"with":[14,251,372],"dramatic":[16],"expansion":[17],"in":[18,38,86,126,149,172,213,221,239,246,258,312,348,359,435,481],"recent":[19],"years.":[20],"Similarly,":[21],"Japanese":[22,77,120],"encephalitis":[23,121],"(JE)":[24],"is":[25,42,84,107,449],"one":[26],"of":[27,31,65,103,161,164,177,197,201,210,243,261,351,362,369,390,407,419,462,471,494],"the":[28,39,104,259,282,349,360,391,420,440,460,469,491],"leading":[29],"causes":[30],"acute":[32],"encephalopathy":[33],"affecting":[34],"children":[35],"and":[36,67,76,91,112,119,135,155,166,180,229,275,315,353,381,405,415,422,452,473],"adolescents":[37],"tropics.":[40],"There":[41],"neither":[43],"an":[44,62,300],"antiviral":[45],"therapy":[46],"nor":[47],"any":[48],"effective":[49,70,179],"vaccine":[50,157,323,482],"available":[51],"for":[52,74,116,144,193,207,325,468,478,490],"dengue.":[53],"Early":[54],"diagnosis":[55,470],"plays":[56],"crucial":[58],"role":[59],"to":[60,68,88,108,131,302,309,320,431,465],"forecast":[61],"early":[63],"warning":[64],"epidemic":[66],"undertake":[69],"vector":[71],"control":[72],"measures":[73],"dengue":[75,118],"encephalitis.":[78],"Envelope":[79],"domain":[80,123],"III":[81,124],"(EDIII)":[82],"protein":[83,138,212,245,255,283,296,330,341,394,409,425,448],"involved":[85],"binding":[87],"host":[89],"receptors":[90],"it":[92],"contains":[93],"speci\ufb01c":[94],"epitopes":[95],"that":[96,437,445],"elicit":[97,453],"virus":[98,475],"neutralizing":[99,455],"antibodies.":[100,456],"The":[101,254,292,388],"objective":[102],"present":[105],"work":[106],"develop":[109,303],"high":[110,133],"yield":[111],"scalable":[113],"production":[114,196,209,493],"process":[115,130,220,235,367,485],"recombinant":[117,173,198,328,392,446,496],"envelope":[122],"proteins":[125,163,464],"Escherichia":[127,174],"coli,":[128],"purification":[129,366],"achieve":[132],"purity":[134,389],"biologically":[136],"active":[137],"as":[139,141,146,184,186,299,343],"well":[140,185],"their":[142,429],"characterization":[143],"use":[145],"diagnostic":[147,280],"reagent":[148],"enzyme":[150,304],"linked":[151,305],"immunosorbent":[152,306],"assay":[153,307],"(ELISA)":[154,308],"possible":[156],"candidate":[158,324],"molecule.":[159],"Expression":[160,242],"EDIII":[162,211,329,340,393,424,447],"JE":[165,472],"viruses":[168],"was":[169,204,224,236,249,256,284,297,331,346,386,395,410,426],"carried":[170,205],"out":[171,206],"coli.":[175,215],"Developments":[176],"cost":[178],"simple":[181],"culture":[182,188,202,230],"media":[183,203,228],"appropriate":[187],"conditions":[189,288],"are":[190],"generally":[191],"favorable":[192],"large":[194],"scale":[195,217,336],"proteins.":[199,498],"Optimization":[200],"enhanced":[208],"E.":[214,222,247],"Laboratory":[216],"batch":[218],"fermentation":[219,234,337],"coli":[223,248],"developed":[225,238],"using":[226,269,289],"optimized":[227,240],"conditions.":[231],"Furthermore,":[232],"fed-batch":[233],"also":[237,487],"medium.":[241],"this":[244,322,408],"induced":[250],"isopropyl":[252],"\u03b2-D-thiogalactoside.":[253],"overexpressed":[257],"form":[260],"insoluble":[262],"inclusion":[263,344],"bodies":[264,345],"(IBs).":[265],"Cells":[266],"were":[267,277],"disrupted":[268],"sonicator":[270],"or":[271,477],"agitator":[272],"bead":[273],"mill":[274],"IBs":[276],"purified.":[278],"For":[279],"studies,":[281,327],"purified":[285,295,423],"under":[286],"denaturing":[287],"affinity":[290,293,373],"chromatography.":[291],"chromatography":[294,376],"used":[298,467],"antigen":[301],"detect":[310],"antibodies":[311,434],"infected":[313],"serum":[314],"CSF":[316],"samples.":[317],"In":[318],"order":[319],"take":[321],"further":[326,479],"produced":[332],"employing":[333],"pilot":[335],"process.":[338],"Recombinant":[339],"expressed":[342],"solubilized":[347],"presence":[350,361],"urea":[352],"renatured":[354],"by":[355,397,412,428],"oncolumn":[356],"refolding":[357,371],"protocol":[358],"glycerol.":[363],"A":[364],"three-step":[365],"comprising":[368],"on-column":[370],"chromatography,":[374],"ion-exchange":[375],"(IEX)":[377],"based":[378,383],"on":[379,384],"salt,":[380],"IEX":[382],"pH":[385],"developed.":[387],"checked":[396],"sodium":[398],"dodecyl":[399],"sulfate":[400],"polyacrylamide":[401],"gel":[402],"electrophoresis":[403],"analysis,":[404],"reactivity":[406],"determined":[411],"Western":[413],"blotting":[414],"ELISA.":[416],"Biological":[417],"function":[418],"refolded":[421],"confirmed":[427],"ability":[430],"generate":[432],"EDIII-specific":[433],"mice":[436],"could":[438],"neutralize":[439],"virus.":[441],"These":[442,457],"findings":[443],"suggest":[444],"highly":[450],"immunogenic":[451],"high-titer":[454],"results":[458],"establish":[459],"application":[461],"these":[463],"be":[466,488],"infection":[476],"studies":[480],"development.":[483],"This":[484],"may":[486],"suitable":[489],"high-yield":[492],"other":[495]},"counts_by_year":[{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
