{"id":"https://openalex.org/W3216614609","doi":"https://doi.org/10.21203/rs.3.rs-1001809/v1","title":"From Hair to Heart: Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Can Simultaneously Differentiate Into Mature Cardiomyocytes and Atrial Myocytes","display_name":"From Hair to Heart: Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Can Simultaneously Differentiate Into Mature Cardiomyocytes and Atrial Myocytes","publication_year":2021,"publication_date":"2021-11-03","ids":{"openalex":"https://openalex.org/W3216614609","doi":"https://doi.org/10.21203/rs.3.rs-1001809/v1","mag":"3216614609"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-1001809/v1","doi":"https://doi.org/10.21203/rs.3.rs-1001809/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-1001809/v1","pdf_url":"https://www.researchsquare.com/article/rs-1001809/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.researchsquare.com/article/rs-1001809/latest.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060990334","display_name":"Michiko Yamane","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Michiko Yamane","raw_affiliation_strings":["Kitasato University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kitasato University School of Medicine","institution_ids":["https://openalex.org/I64189623"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041573827","display_name":"Nanako Takaoka","orcid":"https://orcid.org/0000-0001-5408-7226","observed_orcids":["https://orcid.org/0000-0001-5408-7226"]},"institutions":[{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Nanako Takaoka","raw_affiliation_strings":["Kitasato University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kitasato University School of Medicine","institution_ids":["https://openalex.org/I64189623"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056085252","display_name":"Koya Obara","orcid":"https://orcid.org/0000-0002-5235-3161","observed_orcids":["https://orcid.org/0000-0002-5235-3161"]},"institutions":[{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Koya Obara","raw_affiliation_strings":["Kitasato University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kitasato University School of Medicine","institution_ids":["https://openalex.org/I64189623"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059394545","display_name":"Kyoumi Shirai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kyoumi Shirai","raw_affiliation_strings":["Kitasato University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kitasato University School of Medicine","institution_ids":["https://openalex.org/I64189623"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080333862","display_name":"Ryoichi Aki","orcid":"https://orcid.org/0000-0001-7120-5714","observed_orcids":["https://orcid.org/0000-0001-7120-5714"]},"institutions":[{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Ryoichi Aki","raw_affiliation_strings":["Kitasato University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kitasato University School of Medicine","institution_ids":["https://openalex.org/I64189623"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110848291","display_name":"Yuko Hamada","orcid":"https://orcid.org/0009-0005-5818-5727","observed_orcids":["https://orcid.org/0009-0005-5818-5727"]},"institutions":[{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuko Hamada","raw_affiliation_strings":["Kitasato University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kitasato University School of Medicine","institution_ids":["https://openalex.org/I64189623"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037480411","display_name":"Nobuko Arakawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Nobuko Arakawa","raw_affiliation_strings":["Kitasato University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kitasato University School of Medicine","institution_ids":["https://openalex.org/I64189623"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068311071","display_name":"Manabu Tanaka","orcid":"https://orcid.org/0000-0003-1765-0088","observed_orcids":["https://orcid.org/0000-0003-1765-0088"]},"institutions":[{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Manabu Tanaka","raw_affiliation_strings":["Bio-Imaging Center, Kitasato University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bio-Imaging Center, Kitasato University School of Medicine","institution_ids":["https://openalex.org/I64189623"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040819666","display_name":"Robert M. Hoffman","orcid":"https://orcid.org/0000-0002-0049-0041","observed_orcids":["https://orcid.org/0000-0002-0049-0041"]},"institutions":[{"id":"https://openalex.org/I4210121314","display_name":"AntiCancer (United States)","ror":"https://ror.org/02pp1v282","country_code":"US","type":"company","lineage":["https://openalex.org/I4210121314"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert M. Hoffman","raw_affiliation_strings":["AntiCancer (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AntiCancer (United States)","institution_ids":["https://openalex.org/I4210121314"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5081516321","display_name":"Yasuyuki Amoh","orcid":"https://orcid.org/0000-0002-3522-7868","observed_orcids":["https://orcid.org/0000-0002-3522-7868"]},"institutions":[{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yasuyuki Amoh","raw_affiliation_strings":["Kitasato University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kitasato University School of Medicine","institution_ids":["https://openalex.org/I64189623"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11677","display_name":"Congenital heart defects research","score":0.005799999926239252,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cardiomyocyte-differentiation","display_name":"cardiomyocyte differentiation","score":0.9049999713897705},{"id":"https://openalex.org/keywords/cardiac-regeneration","display_name":"cardiac regeneration","score":0.6620000004768372},{"id":"https://openalex.org/keywords/isoproterenol","display_name":"isoproterenol","score":0.8259999752044678},{"id":"https://openalex.org/keywords/activin-a","display_name":"activin A","score":0.9779999852180481},{"id":"https://openalex.org/keywords/bmp4","display_name":"BMP4","score":0.8600000143051147},{"id":"https://openalex.org/keywords/basic-fibroblast-growth-factor","display_name":"basic fibroblast growth factor","score":0.9300000071525574},{"id":"https://openalex.org/keywords/cyclosporine","display_name":"cyclosporine","score":0.9980000257492065},{"id":"https://openalex.org/keywords/c-kit","display_name":"CD117","score":0.7630000114440918},{"id":"https://openalex.org/keywords/nestin","display_name":"nestin","score":0.5879999995231628}],"concepts":[{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.6907740235328674},{"id":"https://openalex.org/C207200792","wikidata":"https://www.wikidata.org/wiki/Q428914","display_name":"Myocyte","level":2,"score":0.6756218671798706},{"id":"https://openalex.org/C2777216303","wikidata":"https://www.wikidata.org/wiki/Q866324","display_name":"Hair follicle","level":2,"score":0.6594732403755188},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.6544898152351379},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6210912466049194},{"id":"https://openalex.org/C2777738006","wikidata":"https://www.wikidata.org/wiki/Q14906812","display_name":"Nestin","level":4,"score":0.4968321621417999},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.45442771911621094},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4419369101524353},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.3755219578742981},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.355293333530426},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.336497962474823},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.15395790338516235},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.12039783596992493},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0555972158908844},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C136834591","wikidata":"https://www.wikidata.org/wiki/Q2944097","display_name":"Neural stem cell","level":3,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-1001809/v1","doi":"https://doi.org/10.21203/rs.3.rs-1001809/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-1001809/v1","pdf_url":"https://www.researchsquare.com/article/rs-1001809/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-1001809/v1","doi":"https://doi.org/10.21203/rs.3.rs-1001809/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-1001809/v1","pdf_url":"https://www.researchsquare.com/article/rs-1001809/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9763000011444092}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2619405282","display_name":"Establishment of myocardial induction method from autologous hair follicle stem cell-derived stem cells under hypoxic environment and regenerative medicine for heart failure","funder_award_id":"20K17359","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G6443860546","display_name":"Regenerative medicine of spinal cord injury and heart failure using biomaterial including hair-follicle-associated pluripotent (HAP) stem cells","funder_award_id":"19K08781","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3216614609.pdf","grobid_xml":"https://content.openalex.org/works/W3216614609.grobid-xml"},"referenced_works_count":16,"referenced_works":["https://openalex.org/W1539226899","https://openalex.org/W1772643847","https://openalex.org/W1929408314","https://openalex.org/W1979427131","https://openalex.org/W1999160414","https://openalex.org/W2013869667","https://openalex.org/W2026910705","https://openalex.org/W2103637156","https://openalex.org/W2121227333","https://openalex.org/W2126168919","https://openalex.org/W2158482971","https://openalex.org/W2487035651","https://openalex.org/W2954020519","https://openalex.org/W3094090903","https://openalex.org/W3156429697","https://openalex.org/W3186438061"],"related_works":["https://openalex.org/W38277391","https://openalex.org/W4280504052","https://openalex.org/W2323417701","https://openalex.org/W2892333378","https://openalex.org/W2526310237","https://openalex.org/W2526659469","https://openalex.org/W2607334453","https://openalex.org/W2604688691","https://openalex.org/W3013947513","https://openalex.org/W2495914876"],"abstract_inverted_index":{"Abstract":[0],"Hair-follicle-associated":[1],"pluripotent":[2],"(HAP)":[3],"stem":[4,35,71,89],"cells":[5,36,72,90],"express":[6],"nestin,":[7],"and":[8,18,42,62,76,94],"are":[9],"located":[10],"in":[11],"the":[12,27,83],"bulge":[13],"area":[14],"of":[15,24,47,69,82],"hair":[16],"follicles":[17],"can":[19],"differentiate":[20],"to":[21,39,73,92],"numerous":[22],"types":[23],"cells.":[25],"In":[26],"present":[28,84],"study,":[29],"we":[30],"demonstrate":[31],"that":[32,87],"rat":[33],"HAP":[34,70,88],"simultaneously":[37,67],"differentiated":[38],"mature":[40],"cardiomyocytes":[41,75,93],"atrial":[43,78,95],"myocytes.":[44,79],"The":[45,80],"addition":[46],"isoproterenol,":[48],"activin":[49],"A,":[50],"bone":[51],"morphogenetic":[52],"protein":[53],"4":[54],"(BMP":[55],"4),":[56],"basic":[57],"fibroblast":[58],"growth":[59],"factor":[60],"(bFGF),":[61],"cyclosporin":[63],"A":[64],"(CSA),":[65],"induced":[66],"differentiation":[68],"c-kit-positive":[74],"MLC-2a-expressing":[77],"results":[81],"study":[85],"suggest":[86],"differentiating":[91],"myocytes":[96],"have":[97],"future":[98],"clinical":[99],"potential":[100],"for":[101],"heart":[102],"regeneration.":[103]},"counts_by_year":[],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2021-12-06T00:00:00"}