{"id":"https://openalex.org/W3216501971","doi":"https://doi.org/10.1111/jam.15383","title":"Development of a loop-mediated isothermal amplification assay for molecular serotyping of Shigella flexneri Serotypes 2 and Xv","display_name":"Development of a loop-mediated isothermal amplification assay for molecular serotyping of Shigella flexneri Serotypes 2 and Xv","publication_year":2021,"publication_date":"2021-11-25","ids":{"openalex":"https://openalex.org/W3216501971","doi":"https://doi.org/10.1111/jam.15383","mag":"3216501971","pmid":"https://pubmed.ncbi.nlm.nih.gov/34822198"},"language":"en","primary_location":{"id":"doi:10.1111/jam.15383","doi":"https://doi.org/10.1111/jam.15383","is_oa":false,"landing_page_url":"https://doi.org/10.1111/jam.15383","pdf_url":null,"source":{"id":"https://openalex.org/S39794811","display_name":"Journal of Applied Microbiology","issn_l":"1364-5072","issn":["1364-5072","1365-2672"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100402689","display_name":"Sha Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210134288","display_name":"Sanya Central Hospital","ror":"https://ror.org/03t65z939","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210134288"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Sha Li","raw_affiliation_strings":["Central & Clinical Laboratory of Sanya People's Hospital, Sanya People's Hospital, Hainan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central & Clinical Laboratory of Sanya People's Hospital, Sanya People's Hospital, Hainan, China","institution_ids":["https://openalex.org/I4210134288"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081574871","display_name":"Shunshi Ji","orcid":"https://orcid.org/0000-0003-4588-1115","observed_orcids":["https://orcid.org/0000-0003-4588-1115"]},"institutions":[{"id":"https://openalex.org/I184490438","display_name":"Chinese Center For Disease Control and Prevention","ror":"https://ror.org/04wktzw65","country_code":"CN","type":"government","lineage":["https://openalex.org/I184490438","https://openalex.org/I4210127390","https://openalex.org/I4210151987"]},{"id":"https://openalex.org/I4210146685","display_name":"National Institute for Communicable Disease Control and Prevention","ror":"https://ror.org/04f7g6845","country_code":"CN","type":"government","lineage":["https://openalex.org/I184490438","https://openalex.org/I4210127390","https://openalex.org/I4210146685","https://openalex.org/I4210151987"]},{"id":"https://openalex.org/I4391768093","display_name":"State Key Laboratory of Infectious Disease Prevention and Control","ror":"https://ror.org/04bhsv505","country_code":"CN","type":"facility","lineage":["https://openalex.org/I184490438","https://openalex.org/I4210127390","https://openalex.org/I4210151987","https://openalex.org/I4391768093"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shunshi Ji","raw_affiliation_strings":["State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing, China"],"raw_orcid":"https://orcid.org/0000-0003-4588-1115","affiliations":[{"raw_affiliation_string":"State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing, China","institution_ids":["https://openalex.org/I184490438","https://openalex.org/I4210146685","https://openalex.org/I4391768093"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076251001","display_name":"Xiong Zhu","orcid":"https://orcid.org/0000-0002-2594-875X","observed_orcids":["https://orcid.org/0000-0002-2594-875X"]},"institutions":[{"id":"https://openalex.org/I4210134288","display_name":"Sanya Central Hospital","ror":"https://ror.org/03t65z939","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210134288"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiong Zhu","raw_affiliation_strings":["Central & Clinical Laboratory of Sanya People's Hospital, Sanya People's Hospital, Hainan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central & Clinical Laboratory of Sanya People's Hospital, Sanya People's Hospital, Hainan, China","institution_ids":["https://openalex.org/I4210134288"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103759077","display_name":"Hai Chen","orcid":"https://orcid.org/0009-0008-1958-7194","observed_orcids":["https://orcid.org/0009-0008-1958-7194"]},"institutions":[{"id":"https://openalex.org/I4210134288","display_name":"Sanya Central Hospital","ror":"https://ror.org/03t65z939","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210134288"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Hai Chen","raw_affiliation_strings":["Central & Clinical Laboratory of Sanya People's Hospital, Sanya People's Hospital, Hainan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central & Clinical Laboratory of Sanya People's Hospital, Sanya People's Hospital, Hainan, China","institution_ids":["https://openalex.org/I4210134288"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5058233569","display_name":"Dong Jin","orcid":"https://orcid.org/0000-0001-9701-7674","observed_orcids":["https://orcid.org/0000-0001-9701-7674"]},"institutions":[{"id":"https://openalex.org/I184490438","display_name":"Chinese Center For Disease Control and Prevention","ror":"https://ror.org/04wktzw65","country_code":"CN","type":"government","lineage":["https://openalex.org/I184490438","https://openalex.org/I4210127390","https://openalex.org/I4210151987"]},{"id":"https://openalex.org/I4210106542","display_name":"Shanghai Public Health Clinical Center","ror":"https://ror.org/01nnwyz44","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210106542"]},{"id":"https://openalex.org/I4210146685","display_name":"National Institute for Communicable Disease Control and Prevention","ror":"https://ror.org/04f7g6845","country_code":"CN","type":"government","lineage":["https://openalex.org/I184490438","https://openalex.org/I4210127390","https://openalex.org/I4210146685","https://openalex.org/I4210151987"]},{"id":"https://openalex.org/I4391768093","display_name":"State Key Laboratory of Infectious Disease Prevention and Control","ror":"https://ror.org/04bhsv505","country_code":"CN","type":"facility","lineage":["https://openalex.org/I184490438","https://openalex.org/I4210127390","https://openalex.org/I4210151987","https://openalex.org/I4391768093"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Dong Jin","raw_affiliation_strings":["Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai, China","State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing, China"],"raw_orcid":"https://orcid.org/0000-0001-9701-7674","affiliations":[{"raw_affiliation_string":"Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai, China","institution_ids":["https://openalex.org/I4210106542"]},{"raw_affiliation_string":"State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing, China","institution_ids":["https://openalex.org/I184490438","https://openalex.org/I4210146685","https://openalex.org/I4391768093"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5058233569"],"corresponding_institution_ids":["https://openalex.org/I184490438","https://openalex.org/I4210106542","https://openalex.org/I4210146685","https://openalex.org/I4391768093"],"apc_list":{"value":2400,"currency":"GBP","value_usd":3600},"apc_paid":null,"fwci":0.1363,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.3057421,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"132","issue":"4","first_page":"2980","last_page":"2989"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.6977999806404114,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.6977999806404114,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.22419999539852142,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10943","display_name":"Escherichia coli research studies","score":0.05620000138878822,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/shigella-flexneri","display_name":"Shigella flexneri","score":0.9629999995231628},{"id":"https://openalex.org/keywords/serotyping","display_name":"serotyping","score":0.9399999976158142},{"id":"https://openalex.org/keywords/loop-mediated-isothermal-amplification","display_name":"loop-mediated isothermal amplification","score":0.9660000205039978},{"id":"https://openalex.org/keywords/serotype-2","display_name":"serotype 2","score":0.8740000128746033},{"id":"https://openalex.org/keywords/opt","display_name":"OPT","score":0.8339999914169312},{"id":"https://openalex.org/keywords/rapid-molecular-diagnostics","display_name":"rapid molecular diagnostics","score":0.4740000069141388},{"id":"https://openalex.org/keywords/fecal-samples","display_name":"fecal samples","score":0.4620000123977661}],"concepts":[{"id":"https://openalex.org/C2781217973","wikidata":"https://www.wikidata.org/wiki/Q1644417","display_name":"Shigella flexneri","level":4,"score":0.97234046459198},{"id":"https://openalex.org/C10389963","wikidata":"https://www.wikidata.org/wiki/Q848328","display_name":"Serotype","level":2,"score":0.8850830793380737},{"id":"https://openalex.org/C60635243","wikidata":"https://www.wikidata.org/wiki/Q6675780","display_name":"Loop-mediated isothermal amplification","level":3,"score":0.742006778717041},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.6866666078567505},{"id":"https://openalex.org/C2776986154","wikidata":"https://www.wikidata.org/wiki/Q131029","display_name":"Shigella","level":4,"score":0.629364013671875},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5374909043312073},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.44202226400375366},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3840416669845581},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.2634879946708679},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.20518824458122253},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.13979381322860718},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11780327558517456}],"mesh":[{"descriptor_ui":"D012703","descriptor_name":"Serotyping","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D012760","descriptor_name":"Shigella","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012763","descriptor_name":"Shigella flexneri","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D021141","descriptor_name":"Nucleic Acid Amplification Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D025202","descriptor_name":"Molecular Diagnostic Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060885","descriptor_name":"Multiplex Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D065288","descriptor_name":"Serogroup","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/jam.15383","doi":"https://doi.org/10.1111/jam.15383","is_oa":false,"landing_page_url":"https://doi.org/10.1111/jam.15383","pdf_url":null,"source":{"id":"https://openalex.org/S39794811","display_name":"Journal of Applied Microbiology","issn_l":"1364-5072","issn":["1364-5072","1365-2672"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Microbiology","raw_type":"journal-article"},{"id":"pmid:34822198","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34822198","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of applied microbiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.7246000170707703,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1573411577","display_name":null,"funder_award_id":"2019YFC1200505","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"},{"id":"https://openalex.org/G1975698740","display_name":null,"funder_award_id":"SZSM201811071","funder_id":"https://openalex.org/F4320335767","funder_display_name":"Sanming Project of Medicine in Shenzhen"},{"id":"https://openalex.org/G2593153398","display_name":null,"funder_award_id":"2019YFC1200500","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"},{"id":"https://openalex.org/G4868545773","display_name":null,"funder_award_id":"2019HK125","funder_id":"https://openalex.org/F4320330645","funder_display_name":"General Administration of Customs"},{"id":"https://openalex.org/G5636628458","display_name":null,"funder_award_id":"2017FY101202","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"}],"funders":[{"id":"https://openalex.org/F4320330645","display_name":"General Administration of Customs","ror":"https://ror.org/00s255r43"},{"id":"https://openalex.org/F4320335767","display_name":"Sanming Project of Medicine in Shenzhen","ror":null},{"id":"https://openalex.org/F4320335777","display_name":"National Key Research and Development Program of China","ror":null}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":21,"referenced_works":["https://openalex.org/W1859343315","https://openalex.org/W1979974453","https://openalex.org/W1982394927","https://openalex.org/W2041694079","https://openalex.org/W2077977024","https://openalex.org/W2088672819","https://openalex.org/W2090874339","https://openalex.org/W2093874872","https://openalex.org/W2094900351","https://openalex.org/W2105275554","https://openalex.org/W2114053580","https://openalex.org/W2114933883","https://openalex.org/W2119590573","https://openalex.org/W2123227667","https://openalex.org/W2165979400","https://openalex.org/W2298505624","https://openalex.org/W2399416550","https://openalex.org/W2551289541","https://openalex.org/W2594810826","https://openalex.org/W2912603304","https://openalex.org/W6734877151"],"related_works":["https://openalex.org/W3201304018","https://openalex.org/W2762072130","https://openalex.org/W2090874339","https://openalex.org/W2366338016","https://openalex.org/W2182085958","https://openalex.org/W2383716802","https://openalex.org/W2011653702","https://openalex.org/W3108880818","https://openalex.org/W2293752562","https://openalex.org/W2035687501"],"abstract_inverted_index":{"AIMS:":[0],"This":[1,74,122,163],"study":[2,185],"developed":[3],"and":[4,15,22,35,45,51,61,66,84,118,133,143,152,169,176],"evaluated":[5,54],"a":[6],"loop-mediated":[7],"isothermal":[8],"amplification":[9],"(LAMP)":[10],"assay":[11,29,76,102,124,165],"to":[12,127],"simply,":[13],"rapidly":[14],"accurately":[16],"identify":[17,128],"Shigella":[18,64,116],"flexneri":[19,40,60,99,110,130,138,173],"serotypes":[20,57,131,174,193],"2":[21,132,175],"Xv.":[23,177],"METHODS":[24],"AND":[25,179],"RESULTS:":[26],"The":[27,97,183],"LAMP":[28,75,101,123,164,189],"based":[30],"on":[31],"the":[32,159,187],"O-antigen":[33],"synthesis":[34],"modification":[36],"genes":[37],"of":[38,58,115,149,194],"S.":[39,59,98,109,129,137,172,195],"including":[41],"gtrII,":[42],"gtrX,":[43],"opt":[44],"wzx":[46],"was":[47,77,125,186],"developed.":[48],"Its":[49],"specificity":[50,105],"sensitivity":[52],"were":[53,145],"with":[55,147],"19":[56,108],"96":[62,113],"other":[63,119],"species":[65],"bacterial":[67,120],"pathogens":[68],"commonly":[69],"found":[70],"in":[71,141],"stool":[72],"samples.":[73],"completed":[78],"within":[79],"20":[80],"min":[81],"at":[82,90],"61\u00b0C":[83],"could":[85],"detect":[86],"boiled":[87],"DNA":[88],"samples":[89],"concentrations":[91],"as":[92,94],"low":[93],"1":[95],"pg/\u03bcl.":[96],"serotype":[100],"exhibited":[103],"100%":[104],"for":[106,158,191],"detecting":[107],"serotypes,":[111],"no":[112],"strains":[114,139],"spp.":[117],"pathogens.":[121],"used":[126],"Xv":[134],"from":[135],"299":[136],"isolated":[140],"China":[142],"results":[144,157],"consistent":[146],"that":[148],"slide":[150],"agglutination":[151],"multiplex":[153],"polymerase":[154],"chain":[155],"reaction":[156],"same":[160],"isolates.":[161],"CONCLUSIONS:":[162],"may":[166],"facilitate":[167],"rapid":[168],"reliable":[170],"identifying":[171,192],"SIGNIFICANCE":[178],"IMPACT":[180],"OF":[181],"STUDY:":[182],"present":[184],"first":[188],"method":[190],"flexneri.":[196]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2021-12-06T00:00:00"}