{"id":"https://openalex.org/W3216492897","doi":"https://doi.org/10.3389/fbioe.2021.764188","title":"Improved the Activity of Phosphite Dehydrogenase and its Application in Plant Biotechnology","display_name":"Improved the Activity of Phosphite Dehydrogenase and its Application in Plant Biotechnology","publication_year":2021,"publication_date":"2021-11-25","ids":{"openalex":"https://openalex.org/W3216492897","doi":"https://doi.org/10.3389/fbioe.2021.764188","mag":"3216492897","pmid":"https://pubmed.ncbi.nlm.nih.gov/34900961","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8655118"},"language":"en","primary_location":{"id":"doi:10.3389/fbioe.2021.764188","doi":"https://doi.org/10.3389/fbioe.2021.764188","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fbioe.2021.764188","pdf_url":null,"source":{"id":"https://openalex.org/S2595131146","display_name":"Frontiers in Bioengineering and Biotechnology","issn_l":"2296-4185","issn":["2296-4185"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Bioengineering and Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.3389/fbioe.2021.764188","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100638095","display_name":"Tongtong Liu","orcid":"https://orcid.org/0000-0001-9779-7519","observed_orcids":["https://orcid.org/0000-0001-9779-7519"]},"institutions":[{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Tongtong Liu","raw_affiliation_strings":["Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), MOA, Huazhong Agricultural University, Wuhan, China","Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), MOA, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]},{"raw_affiliation_string":"Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067366496","display_name":"Lili Yuan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]},{"id":"https://openalex.org/I4210138501","display_name":"Chinese Academy of Agricultural Sciences","ror":"https://ror.org/0313jb750","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210127390","https://openalex.org/I4210138501","https://openalex.org/I4210151987"]},{"id":"https://openalex.org/I4210142396","display_name":"Oil Crops Research Institute","ror":"https://ror.org/0354r6c10","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210127390","https://openalex.org/I4210138501","https://openalex.org/I4210142396","https://openalex.org/I4210151987"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lili Yuan","raw_affiliation_strings":["Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China","Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]},{"raw_affiliation_string":"Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210138501","https://openalex.org/I4210142396"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091571416","display_name":"Suren Deng","orcid":"https://orcid.org/0000-0002-6422-0171","observed_orcids":["https://orcid.org/0000-0002-6422-0171"]},"institutions":[{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Suren Deng","raw_affiliation_strings":["Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055360649","display_name":"Xiangxian Zhang","orcid":"https://orcid.org/0000-0001-6765-9713","observed_orcids":["https://orcid.org/0000-0001-6765-9713"]},"institutions":[{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]},{"id":"https://openalex.org/I4391768000","display_name":"State Key Laboratory of Agricultural Microbiology","ror":"https://ror.org/003hwx626","country_code":"CN","type":"facility","lineage":["https://openalex.org/I204823248","https://openalex.org/I4391768000"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiangxian Zhang","raw_affiliation_strings":["State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248","https://openalex.org/I4391768000"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089901559","display_name":"Hongmei Cai","orcid":"https://orcid.org/0000-0002-1430-5126","observed_orcids":["https://orcid.org/0000-0002-1430-5126"]},"institutions":[{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Hongmei Cai","raw_affiliation_strings":["Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029673789","display_name":"Guangda Ding","orcid":"https://orcid.org/0000-0003-3702-5087","observed_orcids":["https://orcid.org/0000-0003-3702-5087","https://orcid.org/0009-0001-2589-1538"]},"institutions":[{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Guangda Ding","raw_affiliation_strings":["Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), MOA, Huazhong Agricultural University, Wuhan, China","Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), MOA, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]},{"raw_affiliation_string":"Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090607642","display_name":"Fangsen Xu","orcid":"https://orcid.org/0000-0003-3564-1644","observed_orcids":["https://orcid.org/0000-0003-3564-1644","https://orcid.org/0000-0002-6327-889X"]},"institutions":[{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Fangsen Xu","raw_affiliation_strings":["Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), MOA, Huazhong Agricultural University, Wuhan, China","Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), MOA, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]},{"raw_affiliation_string":"Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077113159","display_name":"Lei Gang Shi","orcid":"https://orcid.org/0000-0002-5312-8521","observed_orcids":["https://orcid.org/0000-0002-5312-8521"]},"institutions":[{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lei Shi","raw_affiliation_strings":["Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), MOA, Huazhong Agricultural University, Wuhan, China","Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), MOA, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]},{"raw_affiliation_string":"Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084689618","display_name":"Gaobing Wu","orcid":"https://orcid.org/0000-0003-4156-5406","observed_orcids":["https://orcid.org/0000-0003-4156-5406"]},"institutions":[{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]},{"id":"https://openalex.org/I4391768000","display_name":"State Key Laboratory of Agricultural Microbiology","ror":"https://ror.org/003hwx626","country_code":"CN","type":"facility","lineage":["https://openalex.org/I204823248","https://openalex.org/I4391768000"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Gaobing Wu","raw_affiliation_strings":["State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248","https://openalex.org/I4391768000"]}]},{"author_position":"last","author":{"id":null,"display_name":"Chuang Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Chuang Wang","raw_affiliation_strings":["Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), MOA, Huazhong Agricultural University, Wuhan, China","Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), MOA, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]},{"raw_affiliation_string":"Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China","institution_ids":["https://openalex.org/I204823248"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5084689618"],"corresponding_institution_ids":["https://openalex.org/I204823248","https://openalex.org/I4391768000"],"apc_list":{"value":2950,"currency":"USD","value_usd":2950},"apc_paid":{"value":2950,"currency":"USD","value_usd":2950},"fwci":0.4336,"has_fulltext":true,"cited_by_count":6,"citation_normalized_percentile":{"value":0.57342275,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"9","issue":null,"first_page":"764188","last_page":"764188"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.9628999829292297,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.9628999829292297,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12186","display_name":"Phosphorus and nutrient management","score":0.01119999960064888,"subfield":{"id":"https://openalex.org/subfields/2311","display_name":"Waste Management and Disposal"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12991","display_name":"Phytase and its Applications","score":0.007400000002235174,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phosphite-dehydrogenase","display_name":"phosphite dehydrogenase","score":1.0},{"id":"https://openalex.org/keywords/phosphorus-fertilizer","display_name":"phosphorus fertilizer","score":0.75},{"id":"https://openalex.org/keywords/directed-evolution","display_name":"directed evolution","score":0.7409999966621399},{"id":"https://openalex.org/keywords/saturation-mutagenesis","display_name":"saturation mutagenesis","score":0.9599999785423279},{"id":"https://openalex.org/keywords/genetically-modified-plants","display_name":"transgenic plants","score":0.781000018119812},{"id":"https://openalex.org/keywords/arabidopsis-thaliana","display_name":"Arabidopsis thaliana","score":0.9210000038146973},{"id":"https://openalex.org/keywords/rice","display_name":"rice","score":0.9850000143051147},{"id":"https://openalex.org/keywords/catalytic-efficiency","display_name":"catalytic efficiency","score":0.7720000147819519},{"id":"https://openalex.org/keywords/phosphorus-deficiency","display_name":"phosphorus deficiency","score":0.4000000059604645}],"concepts":[{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.5530611872673035},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5423726439476013},{"id":"https://openalex.org/C2776317432","wikidata":"https://www.wikidata.org/wiki/Q412547","display_name":"Dehydrogenase","level":3,"score":0.5186045169830322},{"id":"https://openalex.org/C2779491563","wikidata":"https://www.wikidata.org/wiki/Q157892","display_name":"Arabidopsis","level":4,"score":0.5076891183853149},{"id":"https://openalex.org/C2777031885","wikidata":"https://www.wikidata.org/wiki/Q153418","display_name":"Trimethyl phosphite","level":2,"score":0.439778596162796},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.43396273255348206},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4279002249240875},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.4040786325931549},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3974614441394806},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37940847873687744},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.3316076397895813},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.325661301612854},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2243550419807434},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1924481987953186},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.1317678987979889},{"id":"https://openalex.org/C155647269","wikidata":"https://www.wikidata.org/wiki/Q243455","display_name":"Medicinal chemistry","level":1,"score":0.11997225880622864},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07810118794441223},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3389/fbioe.2021.764188","doi":"https://doi.org/10.3389/fbioe.2021.764188","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fbioe.2021.764188","pdf_url":null,"source":{"id":"https://openalex.org/S2595131146","display_name":"Frontiers in Bioengineering and Biotechnology","issn_l":"2296-4185","issn":["2296-4185"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Bioengineering and Biotechnology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8655118","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8655118/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8655118/pdf/","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Bioeng Biotechnol","raw_type":"Text"},{"id":"pmid:34900961","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34900961","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in bioengineering and biotechnology","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:1784fb4a319544698e8ee4e9dacca24e","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/1784fb4a319544698e8ee4e9dacca24e","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fbioe.2021.764188","doi":"https://doi.org/10.3389/fbioe.2021.764188","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fbioe.2021.764188","pdf_url":null,"source":{"id":"https://openalex.org/S2595131146","display_name":"Frontiers in Bioengineering and Biotechnology","issn_l":"2296-4185","issn":["2296-4185"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Bioengineering and Biotechnology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.5666999816894531}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3549164641","display_name":null,"funder_award_id":"31772378","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G5834807989","display_name":null,"funder_award_id":"32072663 31772378","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G8375675150","display_name":null,"funder_award_id":"32072663","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320321540","display_name":"Ministry of Science and Technology of the People's Republic of China","ror":"https://ror.org/027s68j25"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3216492897.pdf","grobid_xml":"https://content.openalex.org/works/W3216492897.grobid-xml"},"referenced_works_count":33,"referenced_works":["https://openalex.org/W75112750","https://openalex.org/W1508200105","https://openalex.org/W1971580698","https://openalex.org/W1972375550","https://openalex.org/W1974724367","https://openalex.org/W1980496938","https://openalex.org/W1981819797","https://openalex.org/W1986555537","https://openalex.org/W2016914398","https://openalex.org/W2061811741","https://openalex.org/W2074295053","https://openalex.org/W2113120245","https://openalex.org/W2115790919","https://openalex.org/W2127129003","https://openalex.org/W2131558643","https://openalex.org/W2159574993","https://openalex.org/W2303647691","https://openalex.org/W2344856356","https://openalex.org/W2409205128","https://openalex.org/W2488681145","https://openalex.org/W2548252348","https://openalex.org/W2555413320","https://openalex.org/W2745260049","https://openalex.org/W2806904689","https://openalex.org/W2914424615","https://openalex.org/W2916022596","https://openalex.org/W2993409148","https://openalex.org/W3004928888","https://openalex.org/W3014478943","https://openalex.org/W3015406324","https://openalex.org/W3140698379","https://openalex.org/W3153033661","https://openalex.org/W4236568477"],"related_works":["https://openalex.org/W3024498857","https://openalex.org/W7165386997","https://openalex.org/W4394846138","https://openalex.org/W1995906680","https://openalex.org/W4409239125","https://openalex.org/W2811373270","https://openalex.org/W1501394508","https://openalex.org/W2095523947","https://openalex.org/W1972375550","https://openalex.org/W2344856356"],"abstract_inverted_index":{"Phosphorus":[0],"(P)":[1],"is":[2,7,161],"a":[3],"nonrenewable":[4],"resource,":[5],"which":[6],"one":[8],"of":[9,73,93,132,141,155],"the":[10,23,27,59,70,90,107,118,122,128,152],"major":[11],"challenges":[12],"for":[13,43,165],"sustainable":[14],"agriculture.":[15],"Although":[16],"phosphite":[17,50,57,142,148,170],"(Phi)":[18],"can":[19,55],"be":[20,32],"absorbed":[21],"by":[22,34,79,86,117],"plant":[24,35,160],"cells":[25],"through":[26],"Pi":[28],"transporters,":[29],"it":[30],"cannot":[31],"metabolized":[33],"and":[36,96,124,136,144],"unable":[37],"to":[38,68,114],"use":[39],"as":[40,58,112,151,171],"P":[41,61,172],"fertilizers":[42],"crops.":[44],"However,":[45],"transgenic":[46,159],"plants":[47],"that":[48],"overexpressed":[49],"dehydrogenase":[51],"(PtxD)":[52],"from":[53,75],"bacteria":[54],"utilize":[56],"sole":[60],"source.":[62],"In":[63],"this":[64],"study,":[65],"we":[66],"aimed":[67],"improve":[69],"catalytic":[71,99,109],"efficiency":[72,100,110,140],"PtxD":[74,94,158],"Ralstonia":[76],"sp.4506":[77],"(PtxDR4506),":[78],"directed":[80],"evolution.":[81],"Five":[82],"mutations":[83],"were":[84],"generated":[85],"saturation":[87],"mutagenesis":[88],"at":[89],"139th":[91],"site":[92],"R4506":[95],"showed":[97,106,138],"higher":[98],"than":[101],"native":[102],"PtxDR4506.":[103],"The":[104],"PtxDQ":[105,133],"highest":[108],"(5.83-fold":[111],"compared":[113],"PtxDR4506)":[115],"contributed":[116],"41.1%":[119],"decrease":[120],"in":[121,127,134],"Km":[123],"2.5-fold":[125],"increase":[126],"kcat":[129],"values.":[130],"Overexpression":[131],"Arabidopsis":[135],"rice":[137],"increased":[139],"utilization":[143],"excellent":[145],"development":[146],"when":[147],"was":[149],"used":[150],"primary":[153],"source":[154],"P.":[156],"High-efficiency":[157],"an":[162],"essential":[163],"prerequisite":[164],"future":[166],"agricultural":[167],"production":[168],"using":[169],"fertilizers.":[173]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":2}],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2021-12-06T00:00:00"}