{"id":"https://openalex.org/W3215517231","doi":"https://doi.org/10.21203/rs.3.rs-1057090/v1","title":"Dysregulation of Transcription Factor EB Contributes to Cell Proliferation, Metastasis and Stemness in Breast Cancer","display_name":"Dysregulation of Transcription Factor EB Contributes to Cell Proliferation, Metastasis and Stemness in Breast Cancer","publication_year":2021,"publication_date":"2021-11-22","ids":{"openalex":"https://openalex.org/W3215517231","doi":"https://doi.org/10.21203/rs.3.rs-1057090/v1","mag":"3215517231"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-1057090/v1","doi":"https://doi.org/10.21203/rs.3.rs-1057090/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-1057090/v1","pdf_url":"https://www.researchsquare.com/article/rs-1057090/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.researchsquare.com/article/rs-1057090/latest.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5054638002","display_name":"Ningwei Fu","orcid":"https://orcid.org/0000-0002-4966-0072","observed_orcids":["https://orcid.org/0000-0002-4966-0072"]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]},{"id":"https://openalex.org/I4210142152","display_name":"ORCID","ror":"https://ror.org/04fa4r544","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210142152"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Ningwei Fu","raw_affiliation_strings":["Dalian Medical College: Dalian Medical University","ORCiD: https://orcid.org/0000-0002-4966-0072"],"raw_orcid":"https://orcid.org/0000-0002-4966-0072","affiliations":[{"raw_affiliation_string":"Dalian Medical College: Dalian Medical University","institution_ids":["https://openalex.org/I191996457"]},{"raw_affiliation_string":"ORCiD: https://orcid.org/0000-0002-4966-0072","institution_ids":["https://openalex.org/I4210142152"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Ning Fan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ning Fan","raw_affiliation_strings":["Dalian Medical College: Dalian Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Medical College: Dalian Medical University","institution_ids":["https://openalex.org/I191996457"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017104954","display_name":"Wenchao Luo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wenchao Luo","raw_affiliation_strings":["Dalian Medical College: Dalian Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Medical College: Dalian Medical University","institution_ids":["https://openalex.org/I191996457"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072131746","display_name":"Lijia Lv","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lijia Lv","raw_affiliation_strings":["Dalian Medical College: Dalian Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Medical College: Dalian Medical University","institution_ids":["https://openalex.org/I191996457"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Jing Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jing Li","raw_affiliation_strings":["Dalian Medical College: Dalian Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Medical College: Dalian Medical University","institution_ids":["https://openalex.org/I191996457"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019539940","display_name":"Jun J. J. Mao","orcid":"https://orcid.org/0000-0001-9229-0380","observed_orcids":["https://orcid.org/0000-0001-9229-0380"]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jun Mao","raw_affiliation_strings":["Dalian Medical College: Dalian Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Medical College: Dalian Medical University","institution_ids":["https://openalex.org/I191996457"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100450471","display_name":"Feifei Li","orcid":"https://orcid.org/0009-0007-0254-0368","observed_orcids":["https://orcid.org/0009-0007-0254-0368"]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Feifei Li","raw_affiliation_strings":["Dalian Medical College: Dalian Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Medical College: Dalian Medical University","institution_ids":["https://openalex.org/I191996457"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067907421","display_name":"Xujiao Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xujiao Wang","raw_affiliation_strings":["Dalian Medical College: Dalian Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Medical College: Dalian Medical University","institution_ids":["https://openalex.org/I191996457"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100327375","display_name":"Xin Zhang","orcid":"https://orcid.org/0000-0001-8956-6024","observed_orcids":["https://orcid.org/0000-0001-8956-6024"]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xin Zhang","raw_affiliation_strings":["Dalian Medical College: Dalian Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Medical College: Dalian Medical University","institution_ids":["https://openalex.org/I191996457"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079772225","display_name":"Fei Xu","orcid":"https://orcid.org/0000-0001-9922-1927","observed_orcids":["https://orcid.org/0000-0001-9922-1927"]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Fei Xu","raw_affiliation_strings":["Dalian Medical College: Dalian Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Medical College: Dalian Medical University","institution_ids":["https://openalex.org/I191996457"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100380342","display_name":"Yan Li","orcid":"https://orcid.org/0000-0002-4110-5955","observed_orcids":["https://orcid.org/0000-0002-4110-5955"]},"institutions":[{"id":"https://openalex.org/I183067930","display_name":"Shanghai Jiao Tong University","ror":"https://ror.org/0220qvk04","country_code":"CN","type":"education","lineage":["https://openalex.org/I183067930"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yan Li","raw_affiliation_strings":["Shanghai Jiao Tong University Medical Library: Shanghai Jiao Tong University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shanghai Jiao Tong University Medical Library: Shanghai Jiao Tong University School of Medicine","institution_ids":["https://openalex.org/I183067930"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101829386","display_name":"Wei Ma","orcid":"https://orcid.org/0000-0002-2672-2533","observed_orcids":["https://orcid.org/0000-0002-2672-2533"]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Wei Ma","raw_affiliation_strings":["College of Basic Medical Sciences, Dalian Medical University"],"raw_orcid":"https://orcid.org/0000-0002-2672-2533","affiliations":[{"raw_affiliation_string":"College of Basic Medical Sciences, Dalian Medical University","institution_ids":["https://openalex.org/I191996457"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5101829386"],"corresponding_institution_ids":["https://openalex.org/I191996457"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.9520999789237976,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.9520999789237976,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.02280000038444996,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":0.004900000058114529,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tfeb","display_name":"TFEB","score":0.8629999756813049},{"id":"https://openalex.org/keywords/breast-cancer","display_name":"breast cancer","score":0.9750000238418579},{"id":"https://openalex.org/keywords/breast-cancer-stem-cells","display_name":"breast cancer stem cells","score":0.8859999775886536},{"id":"https://openalex.org/keywords/autophagy-lysosome-pathway","display_name":"autophagy-lysosome pathway","score":0.8410000205039978},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.8669999837875366},{"id":"https://openalex.org/keywords/metastasis","display_name":"metastasis","score":0.9729999899864197},{"id":"https://openalex.org/keywords/cell-migration-and-invasion","display_name":"cell migration and invasion","score":0.8510000109672546},{"id":"https://openalex.org/keywords/mammosphere-formation","display_name":"mammosphere formation","score":0.8759999871253967},{"id":"https://openalex.org/keywords/stemness","display_name":"stemness","score":0.6370000243186951},{"id":"https://openalex.org/keywords/lysosome-function","display_name":"lysosomal function","score":0.7839999794960022}],"concepts":[{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.7518830299377441},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.6435025930404663},{"id":"https://openalex.org/C2779013556","wikidata":"https://www.wikidata.org/wiki/Q181876","display_name":"Metastasis","level":3,"score":0.6310118436813354},{"id":"https://openalex.org/C530470458","wikidata":"https://www.wikidata.org/wiki/Q128581","display_name":"Breast cancer","level":3,"score":0.6072884202003479},{"id":"https://openalex.org/C2993589390","wikidata":"https://www.wikidata.org/wiki/Q12859063","display_name":"Breast cancer metastasis","level":5,"score":0.4928770065307617},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.428294837474823},{"id":"https://openalex.org/C143998085","wikidata":"https://www.wikidata.org/wiki/Q162555","display_name":"Oncology","level":1,"score":0.4046664535999298},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3754996061325073},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.36407339572906494},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.347320020198822},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.32807475328445435},{"id":"https://openalex.org/C2992691968","wikidata":"https://www.wikidata.org/wiki/Q181876","display_name":"Cancer metastasis","level":4,"score":0.21309462189674377},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.102143794298172},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09498608112335205}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-1057090/v1","doi":"https://doi.org/10.21203/rs.3.rs-1057090/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-1057090/v1","pdf_url":"https://www.researchsquare.com/article/rs-1057090/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-1057090/v1","doi":"https://doi.org/10.21203/rs.3.rs-1057090/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-1057090/v1","pdf_url":"https://www.researchsquare.com/article/rs-1057090/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.9763000011444092,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3215517231.pdf","grobid_xml":"https://content.openalex.org/works/W3215517231.grobid-xml"},"referenced_works_count":54,"referenced_works":["https://openalex.org/W1678795445","https://openalex.org/W1970661950","https://openalex.org/W1975200363","https://openalex.org/W1975823499","https://openalex.org/W1983889487","https://openalex.org/W1989548729","https://openalex.org/W1999306559","https://openalex.org/W2002583936","https://openalex.org/W2005895632","https://openalex.org/W2010608530","https://openalex.org/W2037337578","https://openalex.org/W2038049117","https://openalex.org/W2055498399","https://openalex.org/W2058818863","https://openalex.org/W2077499130","https://openalex.org/W2116505693","https://openalex.org/W2123508600","https://openalex.org/W2125805335","https://openalex.org/W2127714324","https://openalex.org/W2139285220","https://openalex.org/W2141133346","https://openalex.org/W2153824991","https://openalex.org/W2159640574","https://openalex.org/W2187002261","https://openalex.org/W2189480787","https://openalex.org/W2307542306","https://openalex.org/W2492923874","https://openalex.org/W2555959163","https://openalex.org/W2594727603","https://openalex.org/W2620590717","https://openalex.org/W2624576378","https://openalex.org/W2771031544","https://openalex.org/W2788528841","https://openalex.org/W2789162174","https://openalex.org/W2793142753","https://openalex.org/W2852284974","https://openalex.org/W2898653873","https://openalex.org/W2898745977","https://openalex.org/W2900818357","https://openalex.org/W2911073388","https://openalex.org/W2915026088","https://openalex.org/W2938250883","https://openalex.org/W2965577885","https://openalex.org/W2979846121","https://openalex.org/W2991456852","https://openalex.org/W2996589204","https://openalex.org/W3001036028","https://openalex.org/W3031480347","https://openalex.org/W3041217788","https://openalex.org/W3041723582","https://openalex.org/W3047054085","https://openalex.org/W3092177313","https://openalex.org/W3108470142","https://openalex.org/W6652950611"],"related_works":["https://openalex.org/W4406387361","https://openalex.org/W4225404429","https://openalex.org/W7206710669","https://openalex.org/W2555959163","https://openalex.org/W2946424580","https://openalex.org/W2996589204","https://openalex.org/W4399971540","https://openalex.org/W3108470142","https://openalex.org/W2067708052","https://openalex.org/W4362520571"],"abstract_inverted_index":{"Abstract":[0],"Purpose:":[1],"TFEB":[2,43,75,81,131,144,150,167,198],"is":[3,14,37],"a":[4,194],"key":[5],"regulator":[6],"of":[7,34,42,74,114,129,143,161,172,176,189,197],"autophagy-lysosomal":[8],"biogenesis":[9],"pathways,":[10],"while":[11],"its":[12,59],"dysregulation":[13],"highly":[15],"prevalent":[16],"in":[17,44,76,85,147,199,214],"various":[18],"human":[19],"cancers,":[20],"but":[21],"the":[22,40,72,108,127],"specific":[23],"contribution":[24],"to":[25,38,70,106,125],"breast":[26,45,51,77,89,115,133,148,162,200],"cancer":[27,46,52,90,116,134,163,177,201],"remains":[28],"poorly":[29],"understood.":[30],"The":[31,210],"main":[32],"purpose":[33],"this":[35],"study":[36],"explore":[39],"role":[41,196],"proliferation,":[47,109,157],"metastasis":[48],"and":[49,65,87,99,111,159,174,203,205,208,218],"maintaining":[50,215],"stem":[53,135,178],"cells":[54,82],"(BCSCs)":[55],"traits,":[56,217],"thus":[57],"uncovering":[58],"underlying":[60,211],"mechanism.Methods:":[61],"Bioinformatics,":[62],"western":[63],"blotting":[64],"immunohistochemical":[66],"staining":[67,122],"were":[68,83,104,123,184],"applied":[69],"analyze":[71],"expression":[73,142,175],"cancer.":[78,149],"Stable":[79],"down-regulation":[80],"established":[84],"MCF-7":[86],"MDA-MB-231":[88],"cell":[91,179],"lines.":[92],"MTT,":[93],"clone":[94],"formation,":[95,119],"wound":[96],"healing,":[97],"transwell":[98],"3D":[100],"tumor":[101],"invasion":[102,112,160],"assays":[103],"used":[105,124],"evaluate":[107],"migration":[110,158],"ability":[113,171],"cells.":[117,136,164],"Mammosphere":[118],"immunocytochemical":[120],"(ICC)":[121],"detect":[126],"effect":[128],"down-regulating":[130],"on":[132,155],"Results:":[137],"we":[138,192],"demonstrated":[139],"that":[140],"higher":[141],"was":[145],"found":[146],"depletion":[151],"had":[152],"inhibitory":[153],"effects":[154],"cellular":[156],"Moreover,":[165],"knockdown":[166],"decreased":[168,185],"mammosphere":[169],"formation":[170],"BCSCs":[173,206,216],"markers.":[180],"Autophagy-lysosomal":[181],"related":[182],"proteins":[183],"by":[186],"down":[187],"regulation":[188],"TFEB.":[190],"Conclusion:":[191],"uncovered":[193],"critical":[195],"proliferation":[202],"metastasis,":[204],"self-renewal":[207],"stemness.":[209],"mechanisms":[212],"involve":[213],"dysregulating":[219],"lysosome":[220],"functions.":[221]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2021-12-06T00:00:00"}