{"id":"https://openalex.org/W3214685776","doi":null,"title":"Structural and functional studies of Gram -Negative Bacteria Phospholipid Transport Proteins and Human SHP2 Protein","display_name":"Structural and functional studies of Gram -Negative Bacteria Phospholipid Transport Proteins and Human SHP2 Protein","publication_year":2020,"publication_date":"2020-10-01","ids":{"openalex":"https://openalex.org/W3214685776","mag":"3214685776"},"language":"en","primary_location":{"id":"pmh:oai:ueaeprints.uea.ac.uk:82149","doi":null,"is_oa":false,"landing_page_url":"https://ueaeprints.uea.ac.uk/id/eprint/82149/1/James_Coleman_Thesis_FINAL.pdf","pdf_url":"https://ueaeprints.uea.ac.uk/id/eprint/82149/1/James_Coleman_Thesis_FINAL.pdf","source":{"id":"https://openalex.org/S4306400384","display_name":"UEA Digital Repository (University of East Anglia)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1118541","host_organization_name":"University of East Anglia","host_organization_lineage":["https://openalex.org/I1118541"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086392643","display_name":"James L. Coleman","orcid":"https://orcid.org/0000-0001-7430-2587","observed_orcids":["https://orcid.org/0000-0001-7430-2587"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"James L. Coleman","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-7430-2587","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5086392643"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.8657000064849854,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.8657000064849854,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.06840000301599503,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.03060000017285347,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phospholipid-transport","display_name":"phospholipid transport","score":0.9359999895095825},{"id":"https://openalex.org/keywords/gram-negative-bacteria","display_name":"Gram-negative bacteria","score":0.6800000071525574},{"id":"https://openalex.org/keywords/outer-membrane","display_name":"outer membrane","score":0.7429999709129333},{"id":"https://openalex.org/keywords/lipid-asymmetry","display_name":"lipid asymmetry","score":0.7459999918937683},{"id":"https://openalex.org/keywords/lipopolysaccharide-transport","display_name":"lipopolysaccharide transport","score":0.824999988079071},{"id":"https://openalex.org/keywords/shp2","display_name":"SHP2","score":0.781000018119812},{"id":"https://openalex.org/keywords/methylene-blue","display_name":"methylene blue","score":0.8610000014305115},{"id":"https://openalex.org/keywords/pd-1","display_name":"PD-1","score":0.9729999899864197},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.5490000247955322},{"id":"https://openalex.org/keywords/protein-structure","display_name":"protein structure","score":0.414000004529953},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8029999732971191},{"id":"https://openalex.org/keywords/antibiotic-resistance","display_name":"antibiotic resistance","score":0.3400000035762787}],"concepts":[{"id":"https://openalex.org/C201663137","wikidata":"https://www.wikidata.org/wiki/Q21198990","display_name":"Periplasmic space","level":4,"score":0.8335647583007812},{"id":"https://openalex.org/C146587185","wikidata":"https://www.wikidata.org/wiki/Q258248","display_name":"Bacterial outer membrane","level":4,"score":0.8333210349082947},{"id":"https://openalex.org/C2781302388","wikidata":"https://www.wikidata.org/wiki/Q183453","display_name":"Cardiolipin","level":4,"score":0.7599844932556152},{"id":"https://openalex.org/C46371251","wikidata":"https://www.wikidata.org/wiki/Q4886486","display_name":"Cell envelope","level":4,"score":0.720279335975647},{"id":"https://openalex.org/C65871279","wikidata":"https://www.wikidata.org/wiki/Q42884945","display_name":"Inner membrane","level":3,"score":0.6721169352531433},{"id":"https://openalex.org/C2780726299","wikidata":"https://www.wikidata.org/wiki/Q3078751","display_name":"Phosphatidylglycerol","level":5,"score":0.601879894733429},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5208663940429688},{"id":"https://openalex.org/C57581600","wikidata":"https://www.wikidata.org/wiki/Q2449730","display_name":"Transport protein","level":2,"score":0.5001440048217773},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.49448132514953613},{"id":"https://openalex.org/C2781170229","wikidata":"https://www.wikidata.org/wiki/Q426095","display_name":"Phosphatidylethanolamine","level":5,"score":0.48476165533065796},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4718242585659027},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.43801411986351013},{"id":"https://openalex.org/C2776051709","wikidata":"https://www.wikidata.org/wiki/Q632006","display_name":"Gram-negative bacteria","level":4,"score":0.4367803931236267},{"id":"https://openalex.org/C2778918659","wikidata":"https://www.wikidata.org/wiki/Q186915","display_name":"Phospholipid","level":3,"score":0.43195343017578125},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.4149463176727295},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.40682584047317505},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3361009955406189},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.32077041268348694},{"id":"https://openalex.org/C2776330855","wikidata":"https://www.wikidata.org/wiki/Q650187","display_name":"Phosphatidylcholine","level":4,"score":0.18493330478668213},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:ueaeprints.uea.ac.uk:82149","doi":null,"is_oa":false,"landing_page_url":"https://ueaeprints.uea.ac.uk/id/eprint/82149/1/James_Coleman_Thesis_FINAL.pdf","pdf_url":"https://ueaeprints.uea.ac.uk/id/eprint/82149/1/James_Coleman_Thesis_FINAL.pdf","source":{"id":"https://openalex.org/S4306400384","display_name":"UEA Digital Repository (University of East Anglia)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1118541","host_organization_name":"University of East Anglia","host_organization_lineage":["https://openalex.org/I1118541"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:3214685776","doi":null,"is_oa":false,"landing_page_url":"https://ueaeprints.uea.ac.uk/id/eprint/82149/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":109,"referenced_works":["https://openalex.org/W1555008776","https://openalex.org/W1555792171","https://openalex.org/W1580981982","https://openalex.org/W1607921146","https://openalex.org/W1643603004","https://openalex.org/W1705999641","https://openalex.org/W1964082146","https://openalex.org/W1969918683","https://openalex.org/W1973578915","https://openalex.org/W1982889726","https://openalex.org/W1985139403","https://openalex.org/W1986480819","https://openalex.org/W1990697265","https://openalex.org/W1991366673","https://openalex.org/W1993562651","https://openalex.org/W1996607909","https://openalex.org/W1999851893","https://openalex.org/W2004916218","https://openalex.org/W2006922696","https://openalex.org/W2007697641","https://openalex.org/W2013979997","https://openalex.org/W2015384574","https://openalex.org/W2023607853","https://openalex.org/W2024937856","https://openalex.org/W2027249579","https://openalex.org/W2029235012","https://openalex.org/W2030424572","https://openalex.org/W2030632688","https://openalex.org/W2032467549","https://openalex.org/W2049526353","https://openalex.org/W2051925784","https://openalex.org/W2058645085","https://openalex.org/W2062219923","https://openalex.org/W2066002010","https://openalex.org/W2082289040","https://openalex.org/W2085792563","https://openalex.org/W2089353165","https://openalex.org/W2096246094","https://openalex.org/W2098472937","https://openalex.org/W2100100451","https://openalex.org/W2103188931","https://openalex.org/W2105052930","https://openalex.org/W2109531607","https://openalex.org/W2111836375","https://openalex.org/W2111967267","https://openalex.org/W2114335580","https://openalex.org/W2115404371","https://openalex.org/W2117349454","https://openalex.org/W2119582312","https://openalex.org/W2124266141","https://openalex.org/W2128697793","https://openalex.org/W2128744164","https://openalex.org/W2129123584","https://openalex.org/W2130526419","https://openalex.org/W2131778683","https://openalex.org/W2133551372","https://openalex.org/W2136027907","https://openalex.org/W2136860804","https://openalex.org/W2137678717","https://openalex.org/W2144081223","https://openalex.org/W2154139219","https://openalex.org/W2154557455","https://openalex.org/W2154623108","https://openalex.org/W2154714625","https://openalex.org/W2160605524","https://openalex.org/W2163341755","https://openalex.org/W2165134866","https://openalex.org/W2167573033","https://openalex.org/W2171516699","https://openalex.org/W2177070127","https://openalex.org/W2201963902","https://openalex.org/W2212629349","https://openalex.org/W2222655602","https://openalex.org/W2313789413","https://openalex.org/W2475259576","https://openalex.org/W2524697235","https://openalex.org/W2525566551","https://openalex.org/W2533004672","https://openalex.org/W2547310148","https://openalex.org/W2605033818","https://openalex.org/W2609653397","https://openalex.org/W2612429297","https://openalex.org/W2768197926","https://openalex.org/W2783579690","https://openalex.org/W2790891762","https://openalex.org/W2793167197","https://openalex.org/W2883000299","https://openalex.org/W2887452072","https://openalex.org/W2891517856","https://openalex.org/W2894832627","https://openalex.org/W2898146177","https://openalex.org/W2898440791","https://openalex.org/W2899749851","https://openalex.org/W2912990441","https://openalex.org/W2940182637","https://openalex.org/W2950876916","https://openalex.org/W2955652692","https://openalex.org/W2968257264","https://openalex.org/W2972212968","https://openalex.org/W2973457155","https://openalex.org/W2976028181","https://openalex.org/W2995624929","https://openalex.org/W3003540174","https://openalex.org/W3005146385","https://openalex.org/W3010145555","https://openalex.org/W3021650822","https://openalex.org/W3023016632","https://openalex.org/W3023494905","https://openalex.org/W3045016484"],"related_works":["https://openalex.org/W2995624929","https://openalex.org/W4223588022","https://openalex.org/W3155771921","https://openalex.org/W1986480819","https://openalex.org/W2968579141","https://openalex.org/W2806333133","https://openalex.org/W3092234427","https://openalex.org/W2476750730","https://openalex.org/W7099641791","https://openalex.org/W3173742695"],"abstract_inverted_index":{"Gram-Negative":[0],"bacteria":[1],"have":[2,167],"an":[3,7,25,30,54,63,399],"envelope":[4],"composed":[5],"of":[6,53,59,130,160,177,200,250,257,320,349,411],"Inner":[8],"Membrane":[9,13],"(IM)":[10],"and":[11,37,47,62,75,91,139,164,180,207,217,234,240,255,270,310,323,351,368,379,392],"Outer":[12],"(OM)":[14],"with":[15,98,230,336],"a":[16,39,158,223,267,282,321],"periplasmic":[17],"space":[18],"between":[19,88,122,304,366],"the":[20,35,89,92,99,115,123,146,149,175,181,198,211,220,247,258,275,302,305,311,318,331,337,347,364,381,407],"two.":[21],"The":[22,50,81,128,162,192,341],"OM":[23,51,93,147],"is":[24,38,86,140,157,228,261,296,317,393],"asymmetrical":[26],"membrane":[27],"which":[28,84,118,185,401],"forms":[29],"important":[31,242],"protective":[32],"layer":[33],"for":[34,106,226,274,284],"cell":[36],"contributing":[40],"factor":[41],"to":[42,44,111,142,148,204,266,290,300,325,329,354,358,397],"resistances":[43],"toxic":[45],"compounds":[46],"antibiotic":[48],"agents.":[49],"consists":[52],"outer":[55],"leaflet":[56,65],"made":[57],"up":[58],"Lipopolysaccharides":[60],"(LPS),":[61],"inner":[64],"constructed":[66],"from":[67,109,145],"Phospholipids":[68,119],"(PLs),":[69],"predominantly":[70],"Phosphatidylethanolamine":[71],"(PE),":[72],"Phosphatidylglycerol":[73],"(PG)":[74],"Cardiolipin":[76],"(CA)":[77],"in":[78,171,194,232,235,264,413],"Escherichia":[79],"coli.":[80],"manner":[82],"by":[83,117,184,346],"LPS":[85],"transported":[87,121],"IM":[90,110,150],"has":[94,135],"been":[95,136,169],"well":[96,245],"studied,":[97],"Lipopolysaccharide":[100],"transport":[101,188,214],"(Lpt)":[102],"protein":[103,259,309,339],"complex":[104],"responsible":[105],"their":[107],"trafficking":[108],"OM.":[112],"In":[113,219],"contrast,":[114],"pathways":[116,166,215],"are":[120,190],"two":[124],"membranes":[125],"remain":[126],"unclear.":[127],"Maintenance":[129],"lipid":[131],"asymmetry":[132],"(Mla)":[133],"pathway":[134],"partially":[137],"characterised":[138],"thought":[141],"shuttle":[143],"PLs":[144,189],"(the":[151],"retrograde":[152],"direction),":[153],"although":[154],"this":[155,195,291],"directionality":[156],"point":[159],"contention.":[161],"YebST":[163,216],"PqiABC":[165],"also":[168,262,297,371],"implicated":[170],"PL":[172,213],"trafficking,":[173],"but":[174],"structures":[176],"these":[178],"proteins":[179],"associated":[182],"mechanisms":[183],"they":[186],"potentially":[187],"unknown.":[191],"work":[193,348,382],"thesis":[196],"uses":[197,410],"technique":[199,269],"Protein":[201,293],"X-ray":[202,294],"Crystallography":[203,295],"gain":[205],"structural":[206,286],"functional":[208],"insights":[209],"into":[210,363,406],"putative":[212],"PqiABC.":[218],"following":[221],"work,":[222,292],"novel":[224],"structure":[225],"PqiC":[227],"determined,":[229],"preliminary":[231],"vitro":[233],"vivo":[236],"assays":[237],"suggesting":[238],"functionally":[239],"structurally":[241],"residues,":[243],"as":[244,246,281],"protein\u2019s":[248],"capability":[249],"binding":[251,303,357],"PLs.":[252],"Successful":[253],"purification":[254,268],"crystallisation":[256,272],"YebT":[260],"outlined,":[263],"addition":[265],"initial":[271],"trials":[273],"IM-localised":[276],"YebS":[277],"protein,":[278],"highlighting":[279],"it":[280],"target":[283],"future":[285],"studies.":[287],"\\n":[288],"\\nFurther":[289],"here":[298,383],"used":[299],"characterise":[301],"Homo":[306],"sapiens":[307],"SHP2":[308,316,356,367,375,390],"compound":[312],"Methylene":[313],"Blue":[314],"(MB).":[315],"product":[319],"protooncogene":[322],"appears":[324],"allow":[326],"cancer":[327],"cells":[328],"evade":[330],"immune":[332,415],"response":[333],"through":[334],"cooperation":[335],"regulatory":[338],"PD-1.":[340,359],"SHP2-MB":[342],"interaction":[343,365],"was":[344,352,370,376],"identified":[345],"collaborators":[350],"shown":[353],"prevent":[355],"A":[360],"concurrent":[361],"investigation":[362],"PD-1":[369],"carried":[372],"out.":[373],"While":[374],"successfully":[377],"purified":[378],"crystallised,":[380],"shows":[384],"that":[385],"MB":[386,412],"does":[387],"not":[388],"bind":[389],"directly,":[391],"instead":[394],"likely":[395],"binds":[396],"PD-1,":[398],"insight":[400],"will":[402],"inform":[403],"further":[404],"study":[405],"potential":[408],"therapeutic":[409],"PD-1-mediated":[414],"evasion.":[416]},"counts_by_year":[],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2021-11-22T00:00:00"}