{"id":"https://openalex.org/W3211547749","doi":null,"title":"Tyrosyl DNA phosphodiesterase 1 (Tdp1) is a fidelity factor for non-homologous end-joining reactions.","display_name":"Tyrosyl DNA phosphodiesterase 1 (Tdp1) is a fidelity factor for non-homologous end-joining reactions.","publication_year":2008,"publication_date":"2008-05-01","ids":{"openalex":"https://openalex.org/W3211547749","mag":"3211547749"},"language":"en","primary_location":{"id":"mag:3211547749","doi":null,"is_oa":false,"landing_page_url":"https://cancerres.aacrjournals.org/content/68/9_Supplement/5780","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Cancer Research","raw_type":null},"volume":null,"type":"article","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074241171","display_name":"Karim Bahmed","orcid":"https://orcid.org/0000-0001-9854-5452","observed_orcids":["https://orcid.org/0000-0001-9854-5452","https://orcid.org/0000-0002-8932-1331"]},"institutions":[{"id":"https://openalex.org/I1313298211","display_name":"St. Jude Children's Research Hospital","ror":"https://ror.org/02r3e0967","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1313298211","https://openalex.org/I2802152183"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Karim Bahmed","raw_affiliation_strings":["St. Jude Children's Hospital, Memphis, TN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"St. Jude Children's Hospital, Memphis, TN","institution_ids":["https://openalex.org/I1313298211"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072102696","display_name":"Karin C. Nitiss","orcid":"https://orcid.org/0000-0002-3548-5090","observed_orcids":["https://orcid.org/0000-0002-3548-5090"]},"institutions":[{"id":"https://openalex.org/I1313298211","display_name":"St. Jude Children's Research Hospital","ror":"https://ror.org/02r3e0967","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1313298211","https://openalex.org/I2802152183"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Karin C. Nitiss","raw_affiliation_strings":["St. Jude Children's Hospital, Memphis, TN"],"raw_orcid":"https://orcid.org/0000-0002-3548-5090","affiliations":[{"raw_affiliation_string":"St. Jude Children's Hospital, Memphis, TN","institution_ids":["https://openalex.org/I1313298211"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059528417","display_name":"Aman Seth","orcid":"https://orcid.org/0000-0002-6130-7490","observed_orcids":["https://orcid.org/0000-0002-6130-7490"]},"institutions":[{"id":"https://openalex.org/I1313298211","display_name":"St. Jude Children's Research Hospital","ror":"https://ror.org/02r3e0967","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1313298211","https://openalex.org/I2802152183"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aman Seth","raw_affiliation_strings":["St. Jude Children's Hospital, Memphis, TN"],"raw_orcid":"https://orcid.org/0000-0002-6130-7490","affiliations":[{"raw_affiliation_string":"St. Jude Children's Hospital, Memphis, TN","institution_ids":["https://openalex.org/I1313298211"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5022566853","display_name":"John L. Nitiss","orcid":"https://orcid.org/0000-0002-1013-4972","observed_orcids":["https://orcid.org/0000-0002-1013-4972"]},"institutions":[{"id":"https://openalex.org/I1313298211","display_name":"St. Jude Children's Research Hospital","ror":"https://ror.org/02r3e0967","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1313298211","https://openalex.org/I2802152183"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John L. Nitiss","raw_affiliation_strings":["St. Jude Children's Hospital, Memphis, TN"],"raw_orcid":"https://orcid.org/0000-0002-1013-4972","affiliations":[{"raw_affiliation_string":"St. Jude Children's Hospital, Memphis, TN","institution_ids":["https://openalex.org/I1313298211"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1313298211"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.36347222,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"68","issue":null,"first_page":"5780","last_page":"5780"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11845","display_name":"Cancer therapeutics and mechanisms","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11914","display_name":"PARP inhibition in cancer therapy","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tyrosyl-dna-phosphodiesterase-1","display_name":"TDP1","score":0.8679999709129333},{"id":"https://openalex.org/keywords/tyrosyl-dna-phosphodiesterase","display_name":"tyrosyl-DNA phosphodiesterase","score":0.9139999747276306},{"id":"https://openalex.org/keywords/non-homologous-end-joining","display_name":"non-homologous end joining","score":0.9909999966621399},{"id":"https://openalex.org/keywords/double-strand-break-repair","display_name":"DNA double-strand break repair","score":0.9430000185966492},{"id":"https://openalex.org/keywords/repair-fidelity","display_name":"repair fidelity","score":0.5770000219345093},{"id":"https://openalex.org/keywords/nucleotide-incorporation","display_name":"nucleotide incorporation","score":0.6129999756813049},{"id":"https://openalex.org/keywords/ku80","display_name":"Ku80","score":0.8069999814033508},{"id":"https://openalex.org/keywords/dna-ligase-iv","display_name":"DNA ligase IV","score":0.9869999885559082},{"id":"https://openalex.org/keywords/rad52","display_name":"RAD52","score":0.703000009059906}],"concepts":[{"id":"https://openalex.org/C122917832","wikidata":"https://www.wikidata.org/wiki/Q2065945","display_name":"Non-homologous end joining","level":4,"score":0.8499479293823242},{"id":"https://openalex.org/C193104389","wikidata":"https://www.wikidata.org/wiki/Q18032397","display_name":"Ku80","level":5,"score":0.8019405603408813},{"id":"https://openalex.org/C175114707","wikidata":"https://www.wikidata.org/wiki/Q408483","display_name":"DNA ligase","level":3,"score":0.6680601835250854},{"id":"https://openalex.org/C145803527","wikidata":"https://www.wikidata.org/wiki/Q18032396","display_name":"DNA repair protein XRCC4","level":5,"score":0.60211181640625},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.5486794114112854},{"id":"https://openalex.org/C143425029","wikidata":"https://www.wikidata.org/wiki/Q5205747","display_name":"DNA damage","level":3,"score":0.5292633771896362},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5091001391410828},{"id":"https://openalex.org/C74054111","wikidata":"https://www.wikidata.org/wiki/Q5891445","display_name":"Homology directed repair","level":5,"score":0.4953376352787018},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4850696623325348},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4815926253795624},{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.4600816071033478},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.43550291657447815},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4015333354473114},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.32125890254974365},{"id":"https://openalex.org/C104451858","wikidata":"https://www.wikidata.org/wiki/Q2714458","display_name":"Nucleotide excision repair","level":4,"score":0.31436169147491455},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1588321030139923},{"id":"https://openalex.org/C94966510","wikidata":"https://www.wikidata.org/wiki/Q2252764","display_name":"DNA-binding protein","level":4,"score":0.09886819124221802},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:3211547749","doi":null,"is_oa":false,"landing_page_url":"https://cancerres.aacrjournals.org/content/68/9_Supplement/5780","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Cancer Research","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.41999998688697815}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2043287646","https://openalex.org/W2766967220","https://openalex.org/W2915360286","https://openalex.org/W2325733101","https://openalex.org/W2008605706","https://openalex.org/W2164425287","https://openalex.org/W2407010699","https://openalex.org/W2886979167","https://openalex.org/W819339012","https://openalex.org/W3090848763"],"abstract_inverted_index":{"5780":[0],"DNA":[2,30,212,244,344,356,372,411,421,459],"strand":[3,32],"break":[4],"repair":[5,40,133,354],"is":[6,79,260,394,442],"critical":[7,388],"for":[8,81,263,434],"maintaining":[9],"cell":[10],"survival":[11],"following":[12,413],"exposure":[13],"to":[14,55,103,122,166,215,380,450,457],"agents":[15,86],"such":[16,239],"as":[17,240],"ionizing":[18],"radiation":[19],"or":[20,141,243],"cytotoxic":[21],"anti-cancer":[22],"drugs.":[23],"Two":[24],"major":[25],"pathways":[26],"involved":[27],"in":[28,114,151,158,170,178,193,231,252,273,299,392,407,436],"repairing":[29,82],"double":[31],"breaks":[33],"are":[34,58,100,416],"homologous":[35,258],"recombination,":[36],"a":[37,46,64,95,118,144,167,186,211,279,320,395,404,408],"highly":[38],"accurate":[39],"pathway,":[41,224],"and":[42,128,236,423],"non-homologous":[43],"end-joining":[44,127,156],"(NHEJ),":[45],"pathway":[47],"that":[48,52,66,87,107,257,285,313,326,336,368,386],"can":[49,67],"generate":[50],"translocations":[51],"may":[53,446],"lead":[54],"oncogenesis.":[56],"We":[57,105,116,311,333,384],"studying":[59],"tyrosyl-DNA":[60],"phosphodiesterase":[61],"1":[62],"(Tdp1),":[63],"protein":[65],"remove":[68],"phosphotyrosyl-linked":[69],"peptides":[70],"from":[71,85,289],"the":[72,89,98,124,129,132,152,155,200,207,226,267,283,286,314,330,339,387,452],"ends":[73,209,359,375],"of":[74,92,126,131,154,163,188,199,206,341,355,371,390,403,438,454],"DNA.":[75,104],"The":[76,175,197,217,248],"Tdp1p":[77,391],"reaction":[78,90,157],"important":[80],"damage":[83],"arising":[84],"block":[88],"cycle":[91],"topoisomerases":[93],"at":[94],"point":[96],"where":[97],"enzymes":[99,379,456],"covalently":[101],"bound":[102],"found":[106],"yeast":[108,294],"Tdp1":[109,414,435],"has":[110,296],"an":[111,221],"unexpected":[112],"role":[113,433],"NHEJ.":[115],"used":[117],"plasmid":[119],"recircularization":[120],"assay":[121],"assess":[123],"efficiency":[125,153,340],"accuracy":[130],"reaction.":[134],"When":[135],"plasmids":[136,274],"were":[137,181,228,270,317],"linearized":[138],"with":[139,172,204,276,343,346,357,373],"HindIII":[140],"EcoRI":[142],"(generating":[143,278],"5\u2032":[145],"overhang)":[146],"there":[147],"was":[148,202],"no":[149],"change":[150],"\u0394tdp1":[159,179,253,306,337,349],"cells.":[160,196,364],"However,":[161],"loss":[162],"TDP1":[164,235],"led":[165],"large":[168],"increase":[169],"junctions":[171,177],"sequence":[173,198],"alterations.":[174],"altered":[176],"cells":[180,232,255,308],"mainly":[182],"2-4":[183],"nucleotide":[184,264],"insertions,":[185],"type":[187,195,363],"joining":[189,437],"error":[190],"seldom":[191],"seen":[192,230,272],"wild":[194,362],"insertions":[201],"consistent":[203],"filling-in":[205],"cohesive":[208,374],"by":[210,420,428],"polymerase":[213,290],"prior":[214],"ligation.":[216],"errors":[218,227],"depend":[219],"on":[220,319],"intact":[222],"NHEJ":[223,237,301,393],"since":[225],"not":[229,261,271],"lacking":[233],"both":[234],"components":[238],"Ku80":[241],"(YKU80)":[242],"ligase":[245],"IV":[246],"(DNL4).":[247],"addition":[249,268,315,331],"reactions":[250,269,316,415],"occur":[251],"\u0394rad52":[254],"showing":[256],"recombination":[259],"required":[262],"additions.":[265,310],"Also,":[266],"cut":[275],"Pst1":[277],"3\u2032":[280,398,409],"overhang),":[281],"supporting":[282],"hypothesis":[284],"additions":[287,419],"arise":[288],"filling":[291],"in.":[292],"Since":[293,401],"Pol4":[295,327],"been":[297],"implicated":[298],"some":[300],"reactions,":[302],"we":[303],"tested":[304],"whether":[305],"\u0394pol4":[307],"generated":[309],"showed":[312],"dependent":[318],"functional":[321],"POL4":[322],"gene,":[323],"strongly":[324],"suggesting":[325],"carries":[328],"out":[329],"reactions.":[332],"also":[334,447],"find":[335],"influences":[338],"endjoining":[342],"substrates":[345,441],"blunts":[347],"ends.":[348,460],"strains":[350],"exhibit":[351],"more":[352],"efficient":[353],"blunt":[358,439],"than":[360],"do":[361],"Our":[365],"results":[366,406],"indicate":[367],"\u2032\u2032simple":[369],"ligations\u2032\u2032":[370],"frequently":[376],"require":[377],"end-processing":[378],"ensure":[381],"their":[382],"fidelity.":[383],"hypothesize":[385],"activity":[389],"previously":[396],"characterized":[397],"nucleosidase":[399],"activity.":[400],"removal":[402],"nucleoside":[405],"phosphate,":[410],"molecules":[412],"blocked,":[417],"preventing":[418],"polymerases,":[422],"perhaps":[424],"blocking":[425],"nucleolytic":[426],"digestion":[427],"3\u2032-5\u2032":[429],"exonucleases.":[430],"A":[431],"biochemical":[432],"end":[440],"less":[443],"clear,":[444],"but":[445],"be":[448],"related":[449],"controlling":[451],"ability":[453],"different":[455],"process":[458]},"counts_by_year":[],"updated_date":"2026-09-24T07:57:37.273135","created_date":"2021-11-22T00:00:00"}