{"id":"https://openalex.org/W3211035113","doi":"https://doi.org/10.1016/bs.mie.2021.09.001","title":"Improved folding of recombinant protein via co-expression of exogenous chaperones","display_name":"Improved folding of recombinant protein via co-expression of exogenous chaperones","publication_year":2021,"publication_date":"2021-01-01","ids":{"openalex":"https://openalex.org/W3211035113","doi":"https://doi.org/10.1016/bs.mie.2021.09.001","mag":"3211035113","pmid":"https://pubmed.ncbi.nlm.nih.gov/34752283"},"language":"en","primary_location":{"id":"doi:10.1016/bs.mie.2021.09.001","doi":"https://doi.org/10.1016/bs.mie.2021.09.001","is_oa":false,"landing_page_url":"https://doi.org/10.1016/bs.mie.2021.09.001","pdf_url":null,"source":{"id":"https://openalex.org/S182211142","display_name":"Methods in enzymology on CD-ROM/Methods in enzymology","issn_l":"0076-6879","issn":["0076-6879","1079-2376","1557-7988"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319955","host_organization_name":"Academic Press","host_organization_lineage":["https://openalex.org/P4310319955","https://openalex.org/P4310320990"],"host_organization_lineage_names":["Academic Press","Elsevier BV"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Enzymology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4230868431","display_name":"Recombinant Protein Expression: Prokaryotic Hosts and Cell-Free Systems"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071195487","display_name":"Maruda Shanmugasundaram","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I392282","display_name":"University at Albany, State University of New York","ror":"https://ror.org/012zs8222","country_code":"US","type":"education","lineage":["https://openalex.org/I392282"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maruda Shanmugasundaram","raw_affiliation_strings":["Department of Chemistry, University at Albany, State University of New York, Albany, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University at Albany, State University of New York, Albany, NY, United States","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I392282"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109124477","display_name":"Nadejda V. Pavlova","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I126744593","display_name":"University of Maryland, Baltimore","ror":"https://ror.org/04rq5mt64","country_code":"US","type":"education","lineage":["https://openalex.org/I126744593"]},{"id":"https://openalex.org/I4210127065","display_name":"Fidelity Systems (United States)","ror":"https://ror.org/033cp0g08","country_code":"US","type":"company","lineage":["https://openalex.org/I4210127065"]},{"id":"https://openalex.org/I4405261322","display_name":"Institute of Marine and Environmental Technology","ror":"https://ror.org/05431kn12","country_code":"US","type":"facility","lineage":["https://openalex.org/I4405261322"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nadejda V. Pavlova","raw_affiliation_strings":["Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States; Fidelity Systems, Inc., Gaithersburg, MD, United States","Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States","Fidelity Systems, Inc., Gaithersburg, MD, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States; Fidelity Systems, Inc., Gaithersburg, MD, United States","institution_ids":["https://openalex.org/I126744593","https://openalex.org/I4210127065","https://openalex.org/I4405261322"]},{"raw_affiliation_string":"Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States","institution_ids":["https://openalex.org/I126744593","https://openalex.org/I4405261322"]},{"raw_affiliation_string":"Fidelity Systems, Inc., Gaithersburg, MD, United States","institution_ids":["https://openalex.org/I4210127065"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101921678","display_name":"Andrey R. Pavlov","orcid":"https://orcid.org/0000-0003-2854-9911","observed_orcids":["https://orcid.org/0000-0003-2854-9911"]},"institutions":[{"id":"https://openalex.org/I126744593","display_name":"University of Maryland, Baltimore","ror":"https://ror.org/04rq5mt64","country_code":"US","type":"education","lineage":["https://openalex.org/I126744593"]},{"id":"https://openalex.org/I4210127065","display_name":"Fidelity Systems (United States)","ror":"https://ror.org/033cp0g08","country_code":"US","type":"company","lineage":["https://openalex.org/I4210127065"]},{"id":"https://openalex.org/I4405261322","display_name":"Institute of Marine and Environmental Technology","ror":"https://ror.org/05431kn12","country_code":"US","type":"facility","lineage":["https://openalex.org/I4405261322"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrey R. Pavlov","raw_affiliation_strings":["Fidelity Systems, Inc., Gaithersburg, MD, United States; Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States","Fidelity Systems, Inc., Gaithersburg, MD, United States","Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fidelity Systems, Inc., Gaithersburg, MD, United States; Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States","institution_ids":["https://openalex.org/I126744593","https://openalex.org/I4210127065","https://openalex.org/I4405261322"]},{"raw_affiliation_string":"Fidelity Systems, Inc., Gaithersburg, MD, United States","institution_ids":["https://openalex.org/I4210127065"]},{"raw_affiliation_string":"Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States","institution_ids":["https://openalex.org/I126744593","https://openalex.org/I4405261322"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030625987","display_name":"Igor K. Lednev","orcid":"https://orcid.org/0000-0002-6504-531X","observed_orcids":["https://orcid.org/0000-0002-6504-531X"]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I392282","display_name":"University at Albany, State University of New York","ror":"https://ror.org/012zs8222","country_code":"US","type":"education","lineage":["https://openalex.org/I392282"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Igor K. Lednev","raw_affiliation_strings":["Department of Chemistry, University at Albany, State University of New York, Albany, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University at Albany, State University of New York, Albany, NY, United States","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I392282"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5069542454","display_name":"Frank T. Robb","orcid":"https://orcid.org/0000-0001-5833-6496","observed_orcids":["https://orcid.org/0000-0001-5833-6496"]},"institutions":[{"id":"https://openalex.org/I126744593","display_name":"University of Maryland, Baltimore","ror":"https://ror.org/04rq5mt64","country_code":"US","type":"education","lineage":["https://openalex.org/I126744593"]},{"id":"https://openalex.org/I4405261322","display_name":"Institute of Marine and Environmental Technology","ror":"https://ror.org/05431kn12","country_code":"US","type":"facility","lineage":["https://openalex.org/I4405261322"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Frank T. Robb","raw_affiliation_strings":["Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States. Electronic address: frobb@som.umaryland.edu","Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States. Electronic address: frobb@som.umaryland.edu","institution_ids":["https://openalex.org/I126744593","https://openalex.org/I4405261322"]},{"raw_affiliation_string":"Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD, United States","institution_ids":["https://openalex.org/I126744593","https://openalex.org/I4405261322"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5069542454"],"corresponding_institution_ids":["https://openalex.org/I126744593","https://openalex.org/I4405261322"],"apc_list":null,"apc_paid":null,"fwci":2.1059,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.88031126,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"659","issue":null,"first_page":"145","last_page":"170"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.9345999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.9345999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.06270000338554382,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/recombinant-protein-folding","display_name":"recombinant protein folding","score":0.9760000109672546},{"id":"https://openalex.org/keywords/inclusion-bodies","display_name":"inclusion bodies","score":0.6669999957084656},{"id":"https://openalex.org/keywords/protein-misfolding","display_name":"protein misfolding","score":0.6930000185966492},{"id":"https://openalex.org/keywords/green-fluorescent-protein","display_name":"green fluorescent protein","score":0.5730000138282776},{"id":"https://openalex.org/keywords/aequorea-victoria","display_name":"Aequorea victoria","score":0.8759999871253967},{"id":"https://openalex.org/keywords/chaperonin","display_name":"chaperonin","score":0.7710000276565552},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.6779999732971191},{"id":"https://openalex.org/keywords/soluble-expression","display_name":"soluble protein expression","score":0.5260000228881836}],"concepts":[{"id":"https://openalex.org/C203750385","wikidata":"https://www.wikidata.org/wiki/Q1308970","display_name":"Inclusion bodies","level":4,"score":0.7858377695083618},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.7332573533058167},{"id":"https://openalex.org/C204328495","wikidata":"https://www.wikidata.org/wiki/Q847556","display_name":"Protein folding","level":2,"score":0.7259082794189453},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6891973614692688},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.678347110748291},{"id":"https://openalex.org/C2775962898","wikidata":"https://www.wikidata.org/wiki/Q5073152","display_name":"Chaperone (clinical)","level":2,"score":0.6641579270362854},{"id":"https://openalex.org/C98924942","wikidata":"https://www.wikidata.org/wiki/Q16886584","display_name":"Foldase","level":5,"score":0.6168389320373535},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5322157144546509},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.49813365936279297},{"id":"https://openalex.org/C85755625","wikidata":"https://www.wikidata.org/wiki/Q24771589","display_name":"Chaperonin","level":3,"score":0.46655553579330444},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.46355998516082764},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.44574180245399475},{"id":"https://openalex.org/C2777105759","wikidata":"https://www.wikidata.org/wiki/Q133055","display_name":"Aequorea victoria","level":4,"score":0.4313923716545105},{"id":"https://openalex.org/C61397870","wikidata":"https://www.wikidata.org/wiki/Q1069458","display_name":"Chemical chaperone","level":4,"score":0.4223071038722992},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.41261452436447144},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3803682327270508},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.3463855981826782},{"id":"https://openalex.org/C87190427","wikidata":"https://www.wikidata.org/wiki/Q14864994","display_name":"GroEL","level":4,"score":0.224895179271698},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1798025369644165},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017510","descriptor_name":"Protein Folding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018832","descriptor_name":"Molecular Chaperones","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018832","descriptor_name":"Molecular Chaperones","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/bs.mie.2021.09.001","doi":"https://doi.org/10.1016/bs.mie.2021.09.001","is_oa":false,"landing_page_url":"https://doi.org/10.1016/bs.mie.2021.09.001","pdf_url":null,"source":{"id":"https://openalex.org/S182211142","display_name":"Methods in enzymology on CD-ROM/Methods in enzymology","issn_l":"0076-6879","issn":["0076-6879","1079-2376","1557-7988"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319955","host_organization_name":"Academic Press","host_organization_lineage":["https://openalex.org/P4310319955","https://openalex.org/P4310320990"],"host_organization_lineage_names":["Academic Press","Elsevier BV"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Enzymology","raw_type":"book-chapter"},{"id":"pmid:34752283","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34752283","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in enzymology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2091101400","display_name":null,"funder_award_id":"FA9550-07-1-0022","funder_id":"https://openalex.org/F4320338279","funder_display_name":"Air Force Office of Scientific Research"},{"id":"https://openalex.org/G2367261793","display_name":null,"funder_award_id":"21-012","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G6104774460","display_name":null,"funder_award_id":"MCB 98090352","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G7028990616","display_name":null,"funder_award_id":"FA9550-06-1-0020","funder_id":"https://openalex.org/F4320338279","funder_display_name":"Air Force Office of Scientific Research"},{"id":"https://openalex.org/G8052653656","display_name":"ME:  Collaborative Research: Deployment of Enhanced Stress Responses to Improve Recombinant Expression Systems","funder_award_id":"0224717","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320338279","display_name":"Air Force Office of Scientific Research","ror":"https://ror.org/011e9bt93"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":26,"referenced_works":["https://openalex.org/W72883546","https://openalex.org/W610761774","https://openalex.org/W1970756887","https://openalex.org/W1974273851","https://openalex.org/W1975346659","https://openalex.org/W1983130861","https://openalex.org/W1995127855","https://openalex.org/W1995384532","https://openalex.org/W2015544869","https://openalex.org/W2019681615","https://openalex.org/W2036742051","https://openalex.org/W2036746284","https://openalex.org/W2039074823","https://openalex.org/W2046955172","https://openalex.org/W2052817140","https://openalex.org/W2055649671","https://openalex.org/W2058254034","https://openalex.org/W2061476466","https://openalex.org/W2092606978","https://openalex.org/W2098101572","https://openalex.org/W2104943438","https://openalex.org/W2120860614","https://openalex.org/W2127612970","https://openalex.org/W2154106340","https://openalex.org/W2318172186","https://openalex.org/W4210990206"],"related_works":["https://openalex.org/W4225496073","https://openalex.org/W2164343065","https://openalex.org/W4407608260","https://openalex.org/W7076691315","https://openalex.org/W2811476742","https://openalex.org/W150494183","https://openalex.org/W1482642935","https://openalex.org/W4252806788","https://openalex.org/W2127612970","https://openalex.org/W4392286392"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2021-11-08T00:00:00"}