{"id":"https://openalex.org/W3209837036","doi":"https://doi.org/10.1261/rna.078703.121","title":"Identification of high-confidence human poly(A) RNA isoform scaffolds using nanopore sequencing","display_name":"Identification of high-confidence human poly(A) RNA isoform scaffolds using nanopore sequencing","publication_year":2021,"publication_date":"2021-11-02","ids":{"openalex":"https://openalex.org/W3209837036","doi":"https://doi.org/10.1261/rna.078703.121","mag":"3209837036","pmid":"https://pubmed.ncbi.nlm.nih.gov/34728536","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8906549"},"language":"en","primary_location":{"id":"doi:10.1261/rna.078703.121","doi":"https://doi.org/10.1261/rna.078703.121","is_oa":true,"landing_page_url":"https://doi.org/10.1261/rna.078703.121","pdf_url":"http://rnajournal.cshlp.org/content/28/2/162.full.pdf","source":{"id":"https://openalex.org/S187657468","display_name":"RNA","issn_l":"1355-8382","issn":["1355-8382","1469-9001"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RNA","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"http://rnajournal.cshlp.org/content/28/2/162.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044265645","display_name":"Logan Mulroney","orcid":"https://orcid.org/0000-0002-0534-0165","observed_orcids":["https://orcid.org/0000-0002-0534-0165"]},"institutions":[{"id":"https://openalex.org/I185103710","display_name":"University of California, Santa Cruz","ror":"https://ror.org/03s65by71","country_code":"US","type":"education","lineage":["https://openalex.org/I185103710"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Logan Mulroney","raw_affiliation_strings":["Biomolecular Engineering Department, UC Santa Cruz, CA 95064","1Biomolecular Engineering Department, UC Santa Cruz, California 95064, USA"],"raw_orcid":"https://orcid.org/0000-0002-0534-0165","affiliations":[{"raw_affiliation_string":"Biomolecular Engineering Department, UC Santa Cruz, CA 95064","institution_ids":["https://openalex.org/I185103710"]},{"raw_affiliation_string":"1Biomolecular Engineering Department, UC Santa Cruz, California 95064, USA","institution_ids":["https://openalex.org/I185103710"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055731051","display_name":"Madalee G. Wulf","orcid":"https://orcid.org/0000-0002-7601-413X","observed_orcids":["https://orcid.org/0000-0002-7601-413X"]},"institutions":[{"id":"https://openalex.org/I115020962","display_name":"New England Biolabs (United States)","ror":"https://ror.org/04ywg3445","country_code":"US","type":"company","lineage":["https://openalex.org/I115020962"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Madalee G. Wulf","raw_affiliation_strings":["New England Biolabs, Ipswich, MA 01938","2New England Biolabs, Ipswich, Massachusetts 01938, USA"],"raw_orcid":"https://orcid.org/0000-0002-7601-413X","affiliations":[{"raw_affiliation_string":"New England Biolabs, Ipswich, MA 01938","institution_ids":["https://openalex.org/I115020962"]},{"raw_affiliation_string":"2New England Biolabs, Ipswich, Massachusetts 01938, USA","institution_ids":["https://openalex.org/I115020962"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002096304","display_name":"Ira Schildkraut","orcid":"https://orcid.org/0000-0002-1143-6630","observed_orcids":["https://orcid.org/0000-0002-1143-6630"]},"institutions":[{"id":"https://openalex.org/I115020962","display_name":"New England Biolabs (United States)","ror":"https://ror.org/04ywg3445","country_code":"US","type":"company","lineage":["https://openalex.org/I115020962"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ira Schildkraut","raw_affiliation_strings":["New England Biolabs, Ipswich, MA 01938","2New England Biolabs, Ipswich, Massachusetts 01938, USA"],"raw_orcid":"https://orcid.org/0000-0002-1143-6630","affiliations":[{"raw_affiliation_string":"New England Biolabs, Ipswich, MA 01938","institution_ids":["https://openalex.org/I115020962"]},{"raw_affiliation_string":"2New England Biolabs, Ipswich, Massachusetts 01938, USA","institution_ids":["https://openalex.org/I115020962"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056082416","display_name":"George Tzertzinis","orcid":"https://orcid.org/0000-0001-8821-8060","observed_orcids":["https://orcid.org/0000-0001-8821-8060"]},"institutions":[{"id":"https://openalex.org/I115020962","display_name":"New England Biolabs (United States)","ror":"https://ror.org/04ywg3445","country_code":"US","type":"company","lineage":["https://openalex.org/I115020962"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"George Tzertzinis","raw_affiliation_strings":["New England Biolabs, Ipswich, MA 01938","2New England Biolabs, Ipswich, Massachusetts 01938, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"New England Biolabs, Ipswich, MA 01938","institution_ids":["https://openalex.org/I115020962"]},{"raw_affiliation_string":"2New England Biolabs, Ipswich, Massachusetts 01938, USA","institution_ids":["https://openalex.org/I115020962"]}]},{"author_position":"middle","author":{"id":null,"display_name":"John Buswell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I115020962","display_name":"New England Biolabs (United States)","ror":"https://ror.org/04ywg3445","country_code":"US","type":"company","lineage":["https://openalex.org/I115020962"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John Buswell","raw_affiliation_strings":["New England Biolabs, Ipswich, MA 01938","2New England Biolabs, Ipswich, Massachusetts 01938, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"New England Biolabs, Ipswich, MA 01938","institution_ids":["https://openalex.org/I115020962"]},{"raw_affiliation_string":"2New England Biolabs, Ipswich, Massachusetts 01938, USA","institution_ids":["https://openalex.org/I115020962"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018121239","display_name":"Miten Jain","orcid":"https://orcid.org/0000-0002-4571-3982","observed_orcids":["https://orcid.org/0000-0002-4571-3982"]},"institutions":[{"id":"https://openalex.org/I185103710","display_name":"University of California, Santa Cruz","ror":"https://ror.org/03s65by71","country_code":"US","type":"education","lineage":["https://openalex.org/I185103710"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Miten Jain","raw_affiliation_strings":["Biomolecular Engineering Department, UC Santa Cruz, CA 95064","1Biomolecular Engineering Department, UC Santa Cruz, California 95064, USA"],"raw_orcid":"https://orcid.org/0000-0002-4571-3982","affiliations":[{"raw_affiliation_string":"Biomolecular Engineering Department, UC Santa Cruz, CA 95064","institution_ids":["https://openalex.org/I185103710"]},{"raw_affiliation_string":"1Biomolecular Engineering Department, UC Santa Cruz, California 95064, USA","institution_ids":["https://openalex.org/I185103710"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086203077","display_name":"Hugh E. Olsen","orcid":"https://orcid.org/0000-0002-7293-8853","observed_orcids":["https://orcid.org/0000-0002-7293-8853"]},"institutions":[{"id":"https://openalex.org/I185103710","display_name":"University of California, Santa Cruz","ror":"https://ror.org/03s65by71","country_code":"US","type":"education","lineage":["https://openalex.org/I185103710"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hugh Olsen","raw_affiliation_strings":["Biomolecular Engineering Department, UC Santa Cruz, CA 95064","1Biomolecular Engineering Department, UC Santa Cruz, California 95064, USA"],"raw_orcid":"https://orcid.org/0000-0002-7293-8853","affiliations":[{"raw_affiliation_string":"Biomolecular Engineering Department, UC Santa Cruz, CA 95064","institution_ids":["https://openalex.org/I185103710"]},{"raw_affiliation_string":"1Biomolecular Engineering Department, UC Santa Cruz, California 95064, USA","institution_ids":["https://openalex.org/I185103710"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054009720","display_name":"Mark Diekhans","orcid":"https://orcid.org/0000-0002-0430-0989","observed_orcids":["https://orcid.org/0000-0002-0430-0989"]},"institutions":[{"id":"https://openalex.org/I185103710","display_name":"University of California, Santa Cruz","ror":"https://ror.org/03s65by71","country_code":"US","type":"education","lineage":["https://openalex.org/I185103710"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark Diekhans","raw_affiliation_strings":["Genomics Institute, UC Santa Cruz, CA 95064","3Genomics Institute, UC Santa Cruz, California 95064, USA"],"raw_orcid":"https://orcid.org/0000-0002-0430-0989","affiliations":[{"raw_affiliation_string":"Genomics Institute, UC Santa Cruz, CA 95064","institution_ids":["https://openalex.org/I185103710"]},{"raw_affiliation_string":"3Genomics Institute, UC Santa Cruz, California 95064, USA","institution_ids":["https://openalex.org/I185103710"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087842003","display_name":"Ivan Reis Correa Junior","orcid":"https://orcid.org/0000-0002-3169-6878","observed_orcids":["https://orcid.org/0000-0002-3169-6878"]},"institutions":[{"id":"https://openalex.org/I115020962","display_name":"New England Biolabs (United States)","ror":"https://ror.org/04ywg3445","country_code":"US","type":"company","lineage":["https://openalex.org/I115020962"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ivan R. Corr\u00eaa","raw_affiliation_strings":["New England Biolabs, Ipswich, MA 01938","2New England Biolabs, Ipswich, Massachusetts 01938, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"New England Biolabs, Ipswich, MA 01938","institution_ids":["https://openalex.org/I115020962"]},{"raw_affiliation_string":"2New England Biolabs, Ipswich, Massachusetts 01938, USA","institution_ids":["https://openalex.org/I115020962"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012942178","display_name":"Mark A. Akeson","orcid":"https://orcid.org/0000-0001-9392-8075","observed_orcids":["https://orcid.org/0000-0001-9392-8075"]},"institutions":[{"id":"https://openalex.org/I185103710","display_name":"University of California, Santa Cruz","ror":"https://ror.org/03s65by71","country_code":"US","type":"education","lineage":["https://openalex.org/I185103710"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Mark Akeson","raw_affiliation_strings":["Biomolecular Engineering Department, UC Santa Cruz, CA 95064","1Biomolecular Engineering Department, UC Santa Cruz, California 95064, USA"],"raw_orcid":"https://orcid.org/0000-0001-9392-8075","affiliations":[{"raw_affiliation_string":"Biomolecular Engineering Department, UC Santa Cruz, CA 95064","institution_ids":["https://openalex.org/I185103710"]},{"raw_affiliation_string":"1Biomolecular Engineering Department, UC Santa Cruz, California 95064, USA","institution_ids":["https://openalex.org/I185103710"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5064283272","display_name":"Laurence M. Ettwiller","orcid":"https://orcid.org/0000-0002-3957-6539","observed_orcids":["https://orcid.org/0000-0002-3957-6539"]},"institutions":[{"id":"https://openalex.org/I115020962","display_name":"New England Biolabs (United States)","ror":"https://ror.org/04ywg3445","country_code":"US","type":"company","lineage":["https://openalex.org/I115020962"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Laurence Ettwiller","raw_affiliation_strings":["New England Biolabs, Ipswich, MA 01938","2New England Biolabs, Ipswich, Massachusetts 01938, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"New England Biolabs, Ipswich, MA 01938","institution_ids":["https://openalex.org/I115020962"]},{"raw_affiliation_string":"2New England Biolabs, Ipswich, Massachusetts 01938, USA","institution_ids":["https://openalex.org/I115020962"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5012942178","https://openalex.org/A5064283272","https://openalex.org/A5087842003"],"corresponding_institution_ids":["https://openalex.org/I115020962","https://openalex.org/I185103710"],"apc_list":null,"apc_paid":null,"fwci":1.2546,"has_fulltext":true,"cited_by_count":28,"citation_normalized_percentile":{"value":0.79132971,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":95,"max":99},"biblio":{"volume":"28","issue":"2","first_page":"162","last_page":"176"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.6685000061988831,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.6685000061988831,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.2538999915122986,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.01360000018030405,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cage","display_name":"CAGE","score":0.5789999961853027},{"id":"https://openalex.org/keywords/open-chromatin","display_name":"open chromatin","score":0.8050000071525574},{"id":"https://openalex.org/keywords/rna-degradation","display_name":"RNA degradation","score":0.4490000009536743}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9032963514328003},{"id":"https://openalex.org/C126513998","wikidata":"https://www.wikidata.org/wiki/Q8973627","display_name":"Nanopore sequencing","level":4,"score":0.6891060471534729},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6425189971923828},{"id":"https://openalex.org/C53345823","wikidata":"https://www.wikidata.org/wiki/Q5531554","display_name":"Gene isoform","level":3,"score":0.6027984023094177},{"id":"https://openalex.org/C141795571","wikidata":"https://www.wikidata.org/wiki/Q580942","display_name":"Nanopore","level":2,"score":0.5590556859970093},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5585628747940063},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.48917677998542786},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.38223984837532043},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.31658363342285156},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2178289294242859},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.17179864645004272},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.07653868198394775},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000081414","descriptor_name":"Nanopore Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D039104","descriptor_name":"RNA 3' Polyadenylation Signals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059368","descriptor_name":"RNA Isoforms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D059368","descriptor_name":"RNA Isoforms","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D059467","descriptor_name":"Transcriptome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1261/rna.078703.121","doi":"https://doi.org/10.1261/rna.078703.121","is_oa":true,"landing_page_url":"https://doi.org/10.1261/rna.078703.121","pdf_url":"http://rnajournal.cshlp.org/content/28/2/162.full.pdf","source":{"id":"https://openalex.org/S187657468","display_name":"RNA","issn_l":"1355-8382","issn":["1355-8382","1469-9001"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RNA","raw_type":"journal-article"},{"id":"doi:10.1101/2020.11.18.389049","doi":"https://doi.org/10.1101/2020.11.18.389049","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2020.11.18.389049","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2020/11/20/2020.11.18.389049.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:8906549","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8906549","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RNA","raw_type":"Text"},{"id":"pmid:34728536","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34728536","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RNA (New York, N.Y.)","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1261/rna.078703.121","doi":"https://doi.org/10.1261/rna.078703.121","is_oa":true,"landing_page_url":"https://doi.org/10.1261/rna.078703.121","pdf_url":"http://rnajournal.cshlp.org/content/28/2/162.full.pdf","source":{"id":"https://openalex.org/S187657468","display_name":"RNA","issn_l":"1355-8382","issn":["1355-8382","1469-9001"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RNA","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3655240679","display_name":null,"funder_award_id":"GM12878","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G433284018","display_name":null,"funder_award_id":"SC20130149","funder_id":"https://openalex.org/F4320312414","funder_display_name":"Oxford Nanopore Technologies"},{"id":"https://openalex.org/G464362581","display_name":null,"funder_award_id":"U54-HG004555","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G4810982209","display_name":"Integrated human genome annotation: generation of a reference gene set","funder_award_id":"5u54hg004555-04","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G6369178755","display_name":"A Unified Nanopore Platform for Direct Sequencing of Individual Full Length RNA Strands Bearing Modified Nucleotides","funder_award_id":"R01HG010053","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G6455741448","display_name":null,"funder_award_id":"HG010053","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G6674078858","display_name":"GENCODE: comprehensive genome annotation for human and mouse","funder_award_id":"U41HG007234","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G9000867612","display_name":"GENCODE: comprehensive gene annotation for human and mouse","funder_award_id":"U24HG007234","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"}],"funders":[{"id":"https://openalex.org/F4320307991","display_name":"New England Biolabs","ror":"https://ror.org/04ywg3445"},{"id":"https://openalex.org/F4320310094","display_name":"University of Washington","ror":"https://ror.org/00cvxb145"},{"id":"https://openalex.org/F4320312414","display_name":"Oxford Nanopore Technologies","ror":"https://ror.org/04hyfx005"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3209837036.pdf","grobid_xml":"https://content.openalex.org/works/W3209837036.grobid-xml"},"referenced_works_count":52,"referenced_works":["https://openalex.org/W178710137","https://openalex.org/W398308359","https://openalex.org/W1696199194","https://openalex.org/W1964619043","https://openalex.org/W1973931828","https://openalex.org/W1986223165","https://openalex.org/W2007772656","https://openalex.org/W2008934800","https://openalex.org/W2015416439","https://openalex.org/W2036897871","https://openalex.org/W2075181140","https://openalex.org/W2092977521","https://openalex.org/W2098832713","https://openalex.org/W2104005232","https://openalex.org/W2108138064","https://openalex.org/W2108388593","https://openalex.org/W2112113834","https://openalex.org/W2116194397","https://openalex.org/W2129055445","https://openalex.org/W2143513235","https://openalex.org/W2160444236","https://openalex.org/W2166810745","https://openalex.org/W2169456326","https://openalex.org/W2173732482","https://openalex.org/W2180304330","https://openalex.org/W2236822143","https://openalex.org/W2263197966","https://openalex.org/W2341539131","https://openalex.org/W2767826728","https://openalex.org/W2775755225","https://openalex.org/W2789843538","https://openalex.org/W2797911854","https://openalex.org/W2807138843","https://openalex.org/W2891014523","https://openalex.org/W2894854766","https://openalex.org/W2898598946","https://openalex.org/W2905146042","https://openalex.org/W2936958295","https://openalex.org/W2949707636","https://openalex.org/W2950063384","https://openalex.org/W2951278111","https://openalex.org/W2952440593","https://openalex.org/W2956027726","https://openalex.org/W2980708325","https://openalex.org/W2988267788","https://openalex.org/W2989499631","https://openalex.org/W2995527562","https://openalex.org/W2999689892","https://openalex.org/W3011428862","https://openalex.org/W3026218458","https://openalex.org/W3216640235","https://openalex.org/W4300118964"],"related_works":["https://openalex.org/W3135771840","https://openalex.org/W2989499631","https://openalex.org/W4252675765","https://openalex.org/W3195602130","https://openalex.org/W7219885609","https://openalex.org/W4409157129","https://openalex.org/W3149519053","https://openalex.org/W4386889285","https://openalex.org/W6945296625","https://openalex.org/W3195224616"],"abstract_inverted_index":{"ABSTRACT":[0],"Nanopore":[1],"sequencing":[2,117],"devices":[3],"read":[4],"individual":[5,72,155],"RNA":[6,29,50,75,83,88,158,183,193],"strands":[7,89],"directly.":[8],"This":[9,34,110],"facilitates":[10],"identification":[11,121],"of":[12,27,42,122,161,190],"exon":[13],"linkages":[14],"and":[15,24,46,54,62,118],"nucleotide":[16],"modifications;":[17],"however,":[18],"using":[19],"conventional":[20],"methods":[21],"the":[22,40,43,98,123,188],"5\u2032":[23,91,115,124],"3\u2032":[25,145],"ends":[26],"poly(A)":[28],"cannot":[30],"be":[31],"identified":[32,153,182],"unambiguously.":[33],"is":[35],"due":[36],"in":[37,47,52,55],"part":[38,48],"to":[39,49,70,86,106,165,179],"architecture":[41],"nanopore/enzyme-motor":[44],"complex,":[45],"degradation":[51],"vivo":[53],"vitro":[56],"that":[57,175],"can":[58],"obscure":[59],"transcription":[60],"start":[61],"end":[63,116],"sites.":[64],"In":[65],"this":[66],"study,":[67],"we":[68,96,136,152],"aimed":[69],"identify":[71,87],"full-length":[73,157],"human":[74],"isoform":[76],"scaffolds":[77,171],"among":[78,131],"\u223c4":[79],"million":[80],"nanopore":[81,134],"poly(A)-selected":[82],"reads.":[84],"First,":[85],"bearing":[90],"m":[92,125],"7":[93,126],"G":[94,127],"caps,":[95],"exchanged":[97],"biological":[99],"cap":[100,104],"for":[101,138],"a":[102,107,144],"modified":[103],"attached":[105],"45-nucleotide":[108],"oligomer.":[109],"oligomer":[111],"adaptation":[112],"method":[113],"improved":[114],"ensured":[119],"correct":[120],"capped":[128],"ends.":[129],"Second,":[130],"these":[132,149,191],"5\u2032-capped":[133],"reads,":[135],"screened":[137],"ionic":[139],"current":[140],"signatures":[141],"consistent":[142],"with":[143],"polyadenylation":[146],"site.":[147],"Combining":[148],"two":[150],"steps,":[151],"294,107":[154],"high-confidence":[156,192],"scaffolds,":[159],"most":[160],"which":[162],"(257,721)":[163],"aligned":[164],"protein-coding":[166],"genes.":[167],"Of":[168],"these,":[169],"4,876":[170],"indicated":[172],"unannotated":[173],"isoforms":[174],"were":[176],"often":[177],"internal":[178],"longer,":[180],"previously":[181],"isoforms.":[184],"Orthogonal":[185],"data":[186],"confirmed":[187],"validity":[189],"scaffolds.":[194]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2021-11-08T00:00:00"}