{"id":"https://openalex.org/W3206762952","doi":"https://doi.org/10.1002/mas.21738","title":"Enriching extracellular vesicles for mass spectrometry","display_name":"Enriching extracellular vesicles for mass spectrometry","publication_year":2021,"publication_date":"2021-10-11","ids":{"openalex":"https://openalex.org/W3206762952","doi":"https://doi.org/10.1002/mas.21738","mag":"3206762952","pmid":"https://pubmed.ncbi.nlm.nih.gov/34632607"},"language":"en","primary_location":{"id":"doi:10.1002/mas.21738","doi":"https://doi.org/10.1002/mas.21738","is_oa":false,"landing_page_url":"https://doi.org/10.1002/mas.21738","pdf_url":null,"source":{"id":"https://openalex.org/S69764086","display_name":"Mass Spectrometry Reviews","issn_l":"0277-7037","issn":["0277-7037","1098-2787"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mass Spectrometry Reviews","raw_type":"journal-article"},"volume":null,"type":"review","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5030033652","display_name":"Jordan B. Burton","orcid":"https://orcid.org/0000-0002-5238-0872","observed_orcids":["https://orcid.org/0000-0002-5238-0872"]},"institutions":[{"id":"https://openalex.org/I185443292","display_name":"Wayne State University","ror":"https://ror.org/01070mq45","country_code":"US","type":"education","lineage":["https://openalex.org/I185443292"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jordan B. Burton","raw_affiliation_strings":["Institute of Environmental Health Sciences Wayne State University Detroit Michigan USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Environmental Health Sciences Wayne State University Detroit Michigan USA","institution_ids":["https://openalex.org/I185443292"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007165478","display_name":"Nicholas J. Carruthers","orcid":"https://orcid.org/0000-0003-3070-2057","observed_orcids":["https://orcid.org/0000-0003-3070-2057"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nicholas J. Carruthers","raw_affiliation_strings":["University of Michigan Ann Arbor Michigan USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Michigan Ann Arbor Michigan USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5049983845","display_name":"Paul M. Stemmer","orcid":"https://orcid.org/0000-0003-3315-9846","observed_orcids":["https://orcid.org/0000-0003-3315-9846"]},"institutions":[{"id":"https://openalex.org/I185443292","display_name":"Wayne State University","ror":"https://ror.org/01070mq45","country_code":"US","type":"education","lineage":["https://openalex.org/I185443292"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Paul M. Stemmer","raw_affiliation_strings":["Institute of Environmental Health Sciences Wayne State University Detroit Michigan USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Environmental Health Sciences Wayne State University Detroit Michigan USA","institution_ids":["https://openalex.org/I185443292"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5049983845"],"corresponding_institution_ids":["https://openalex.org/I185443292"],"apc_list":{"value":4000,"currency":"USD","value_usd":4000},"apc_paid":null,"fwci":0.6549,"has_fulltext":false,"cited_by_count":46,"citation_normalized_percentile":{"value":0.5614959,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":100},"biblio":{"volume":"42","issue":"2","first_page":"779","last_page":"795"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/extracellular-vesicles","display_name":"extracellular vesicles","score":0.9959999918937683},{"id":"https://openalex.org/keywords/exosomes","display_name":"exosomes","score":0.9950000047683716},{"id":"https://openalex.org/keywords/exosome-enrichment","display_name":"exosome enrichment","score":0.7649999856948853},{"id":"https://openalex.org/keywords/mass-spectrometry-sample-preparation","display_name":"mass spectrometry sample preparation","score":0.7360000014305115},{"id":"https://openalex.org/keywords/biomarkers","display_name":"biomarkers","score":0.6639999747276306},{"id":"https://openalex.org/keywords/plasma-proteins","display_name":"plasma proteins","score":0.5979999899864197},{"id":"https://openalex.org/keywords/ultracentrifugation","display_name":"ultracentrifugation","score":0.781000018119812},{"id":"https://openalex.org/keywords/chromatographic-separation","display_name":"chromatographic separation","score":0.8560000061988831},{"id":"https://openalex.org/keywords/hydrophobic-interaction-chromatography","display_name":"hydrophobic interaction chromatography","score":0.7879999876022339}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9166887998580933},{"id":"https://openalex.org/C20518536","wikidata":"https://www.wikidata.org/wiki/Q6840469","display_name":"Microvesicles","level":4,"score":0.916103720664978},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.7762573957443237},{"id":"https://openalex.org/C2781261824","wikidata":"https://www.wikidata.org/wiki/Q903634","display_name":"Exosome","level":5,"score":0.6893909573554993},{"id":"https://openalex.org/C54689828","wikidata":"https://www.wikidata.org/wiki/Q2029259","display_name":"Ultracentrifuge","level":2,"score":0.6399483680725098},{"id":"https://openalex.org/C79747257","wikidata":"https://www.wikidata.org/wiki/Q189814","display_name":"Endocytic cycle","level":4,"score":0.6394579410552979},{"id":"https://openalex.org/C2908689518","wikidata":"https://www.wikidata.org/wiki/Q21097485","display_name":"Extracellular vesicle","level":5,"score":0.5659177899360657},{"id":"https://openalex.org/C2992929900","wikidata":"https://www.wikidata.org/wiki/Q21097485","display_name":"Extracellular vesicles","level":2,"score":0.5526618361473083},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.5268650650978088},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.40683966875076294},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3404539227485657},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3078380227088928},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.24059665203094482},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.14437595009803772},{"id":"https://openalex.org/C28005876","wikidata":"https://www.wikidata.org/wiki/Q189814","display_name":"Endocytosis","level":3,"score":0.141431987285614},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.07294049859046936},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000067128","descriptor_name":"Extracellular Vesicles","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001798","descriptor_name":"Blood Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014461","descriptor_name":"Ultracentrifugation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055354","descriptor_name":"Exosomes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/mas.21738","doi":"https://doi.org/10.1002/mas.21738","is_oa":false,"landing_page_url":"https://doi.org/10.1002/mas.21738","pdf_url":null,"source":{"id":"https://openalex.org/S69764086","display_name":"Mass Spectrometry Reviews","issn_l":"0277-7037","issn":["0277-7037","1098-2787"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mass Spectrometry Reviews","raw_type":"journal-article"},{"id":"pmid:34632607","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34632607","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mass spectrometry reviews","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":77,"referenced_works":["https://openalex.org/W1762620449","https://openalex.org/W1888751811","https://openalex.org/W1967421034","https://openalex.org/W1987604193","https://openalex.org/W1989277387","https://openalex.org/W1995875735","https://openalex.org/W2021065026","https://openalex.org/W2044638539","https://openalex.org/W2059162472","https://openalex.org/W2061964401","https://openalex.org/W2068873393","https://openalex.org/W2072630322","https://openalex.org/W2087814846","https://openalex.org/W2094229819","https://openalex.org/W2097774418","https://openalex.org/W2102416146","https://openalex.org/W2103872250","https://openalex.org/W2145629087","https://openalex.org/W2170700901","https://openalex.org/W2170852633","https://openalex.org/W2259481044","https://openalex.org/W2290725277","https://openalex.org/W2338060077","https://openalex.org/W2441633445","https://openalex.org/W2592995008","https://openalex.org/W2613629789","https://openalex.org/W2738491178","https://openalex.org/W2741588972","https://openalex.org/W2753882592","https://openalex.org/W2759081117","https://openalex.org/W2759592955","https://openalex.org/W2762010667","https://openalex.org/W2763697797","https://openalex.org/W2767588247","https://openalex.org/W2770747919","https://openalex.org/W2786369638","https://openalex.org/W2789893810","https://openalex.org/W2790701366","https://openalex.org/W2791016239","https://openalex.org/W2801497388","https://openalex.org/W2808817086","https://openalex.org/W2886527502","https://openalex.org/W2886718156","https://openalex.org/W2891272237","https://openalex.org/W2896433162","https://openalex.org/W2900756811","https://openalex.org/W2904672292","https://openalex.org/W2904772677","https://openalex.org/W2908122377","https://openalex.org/W2918972495","https://openalex.org/W2921054831","https://openalex.org/W2931418311","https://openalex.org/W2937573806","https://openalex.org/W2964206917","https://openalex.org/W2964576521","https://openalex.org/W2970230025","https://openalex.org/W2976994348","https://openalex.org/W2982072667","https://openalex.org/W3004570579","https://openalex.org/W3017443898","https://openalex.org/W3030744287","https://openalex.org/W3035338775","https://openalex.org/W3047244309","https://openalex.org/W3080249817","https://openalex.org/W3081011277","https://openalex.org/W3100197798","https://openalex.org/W3107527779","https://openalex.org/W3108227279","https://openalex.org/W3108965324","https://openalex.org/W3133874665","https://openalex.org/W3137380305","https://openalex.org/W3143289476","https://openalex.org/W3148560233","https://openalex.org/W3159493458","https://openalex.org/W3197672348","https://openalex.org/W4233698560","https://openalex.org/W6647548779"],"related_works":["https://openalex.org/W3214359165","https://openalex.org/W2121875081","https://openalex.org/W2756787067","https://openalex.org/W3176710204","https://openalex.org/W2761680614","https://openalex.org/W2756293416","https://openalex.org/W2596974594","https://openalex.org/W3197241391","https://openalex.org/W2762605995","https://openalex.org/W7125421668"],"abstract_inverted_index":{"Extracellular":[0],"vesicles":[1,99],"from":[2,66,85,125],"plasma,":[3],"other":[4,93],"body":[5],"fluids":[6],"and":[7,35,60,92,140],"cell":[8],"culture":[9],"media":[10],"hold":[11,54],"great":[12],"promise":[13],"in":[14,20,31,90],"the":[15,22,32,58,64,86,98],"search":[16],"for":[17,48,102,123],"biomarkers.":[18],"Exosomes":[19],"particular,":[21],"vesicle":[23],"type":[24],"that":[25,135,141],"is":[26,76,100,138],"secreted":[27],"after":[28],"being":[29],"produced":[30],"endocytic":[33],"pathway":[34],"having":[36],"a":[37,46,110],"diameter":[38],"of":[39,63,74,97,114,120],"30-150":[40],"nm,":[41],"are":[42,69,148],"considered":[43],"to":[44,53,78,82,145],"be":[45],"conveyance":[47],"signaling":[49],"molecules":[50],"and,":[51],"therefore,":[52],"valuable":[55],"information":[56],"regarding":[57],"health":[59],"activity":[61],"status":[62],"cells":[65],"which":[67],"they":[68,107],"released.":[70],"The":[71,95,118],"vesicular":[72],"nature":[73],"exosomes":[75,124],"central":[77],"all":[79,115],"methods":[80],"used":[81],"separate":[83],"them":[84],"highly":[87],"abundant":[88],"proteins":[89],"plasma":[91,116],"fluids.":[94],"enrichment":[96,137,147],"essential":[101],"mass":[103],"spectrometry-based":[104],"analysis":[105],"as":[106],"represent":[108],"only":[109],"very":[111],"small":[112],"component":[113],"proteins.":[117],"progression":[119],"isolation":[121],"techniques":[122],"ultracentrifugation":[126],"through":[127],"chromatographic":[128],"separation":[129],"using":[130],"hydrophobic":[131],"packing":[132],"materials":[133],"shows":[134],"effective":[136],"possible":[139],"high":[142],"throughput":[143],"approaches":[144],"exosome":[146],"achievable.":[149]},"counts_by_year":[{"year":2026,"cited_by_count":7},{"year":2025,"cited_by_count":15},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":9},{"year":2022,"cited_by_count":8},{"year":2021,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2021-10-25T00:00:00"}