{"id":"https://openalex.org/W3205837077","doi":"https://doi.org/10.1039/d1cb00118c","title":"Activation of a G protein-coupled receptor through indirect antibody-mediated tethering of ligands","display_name":"Activation of a G protein-coupled receptor through indirect antibody-mediated tethering of ligands","publication_year":2021,"publication_date":"2021-01-01","ids":{"openalex":"https://openalex.org/W3205837077","doi":"https://doi.org/10.1039/d1cb00118c","mag":"3205837077","pmid":"https://pubmed.ncbi.nlm.nih.gov/34977584","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8637866"},"language":"en","primary_location":{"id":"doi:10.1039/d1cb00118c","doi":"https://doi.org/10.1039/d1cb00118c","is_oa":true,"landing_page_url":"https://doi.org/10.1039/d1cb00118c","pdf_url":"https://pubs.rsc.org/en/content/articlepdf/2021/cb/d1cb00118c","source":{"id":"https://openalex.org/S4210222191","display_name":"RSC Chemical Biology","issn_l":"2633-0679","issn":["2633-0679"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","norway-1","oarl","poland-20","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RSC Chemical Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://pubs.rsc.org/en/content/articlepdf/2021/cb/d1cb00118c","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5018356508","display_name":"Ross W. Cheloha","orcid":"https://orcid.org/0000-0001-9871-8333","observed_orcids":["https://orcid.org/0000-0001-9871-8333"]},"institutions":[{"id":"https://openalex.org/I1288882113","display_name":"Boston Children's Hospital","ror":"https://ror.org/00dvg7y05","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1288882113"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ross W. Cheloha","raw_affiliation_strings":["Program in Cellular and Molecular Medicine, Boston Children's Hospital, 1 Blackfan Circle, Boston, MA, 02115, USA"],"raw_orcid":"https://orcid.org/0000-0001-9871-8333","affiliations":[{"raw_affiliation_string":"Program in Cellular and Molecular Medicine, Boston Children's Hospital, 1 Blackfan Circle, Boston, MA, 02115, USA","institution_ids":["https://openalex.org/I1288882113"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039071922","display_name":"Fabian A. Fischer","orcid":"https://orcid.org/0000-0002-4116-9879","observed_orcids":["https://orcid.org/0000-0002-4116-9879"]},"institutions":[{"id":"https://openalex.org/I1288882113","display_name":"Boston Children's Hospital","ror":"https://ror.org/00dvg7y05","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1288882113"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Fabian A. Fischer","raw_affiliation_strings":["Program in Cellular and Molecular Medicine, Boston Children's Hospital, 1 Blackfan Circle, Boston, MA, 02115, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Program in Cellular and Molecular Medicine, Boston Children's Hospital, 1 Blackfan Circle, Boston, MA, 02115, USA","institution_ids":["https://openalex.org/I1288882113"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015542125","display_name":"Thomas James Gardella","orcid":"https://orcid.org/0000-0002-2163-5772","observed_orcids":["https://orcid.org/0000-0002-2163-5772"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas J. Gardella","raw_affiliation_strings":["Massachusetts General Hospital and Harvard Medical School, 50 Blossom Street, Boston, MA, 02114, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Massachusetts General Hospital and Harvard Medical School, 50 Blossom Street, Boston, MA, 02114, USA","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I4210087915"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5081514135","display_name":"Hidde L. Ploegh","orcid":"https://orcid.org/0000-0002-1090-6071","observed_orcids":["https://orcid.org/0000-0002-1090-6071"]},"institutions":[{"id":"https://openalex.org/I1288882113","display_name":"Boston Children's Hospital","ror":"https://ror.org/00dvg7y05","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1288882113"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Hidde L. Ploegh","raw_affiliation_strings":["Program in Cellular and Molecular Medicine, Boston Children's Hospital, 1 Blackfan Circle, Boston, MA, 02115, USA"],"raw_orcid":"https://orcid.org/0000-0002-1090-6071","affiliations":[{"raw_affiliation_string":"Program in Cellular and Molecular Medicine, Boston Children's Hospital, 1 Blackfan Circle, Boston, MA, 02115, USA","institution_ids":["https://openalex.org/I1288882113"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5018356508","https://openalex.org/A5081514135"],"corresponding_institution_ids":["https://openalex.org/I1288882113"],"apc_list":{"value":1600,"currency":"GBP","value_usd":1962},"apc_paid":{"value":1600,"currency":"GBP","value_usd":1962},"fwci":0.9408,"has_fulltext":true,"cited_by_count":16,"citation_normalized_percentile":{"value":0.72347225,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"2","issue":"6","first_page":"1692","last_page":"1700"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.6791999936103821,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.6791999936103821,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.28200000524520874,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.01140000019222498,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/g-protein-coupled-receptors","display_name":"G protein-coupled receptors","score":0.9940000176429749},{"id":"https://openalex.org/keywords/nanobodies","display_name":"nanobodies","score":0.8510000109672546},{"id":"https://openalex.org/keywords/site-specific-functionalization","display_name":"site-specific functionalization","score":0.734000027179718},{"id":"https://openalex.org/keywords/receptor-activation","display_name":"receptor activation","score":0.7059999704360962},{"id":"https://openalex.org/keywords/cell-surface-proteins","display_name":"cell surface proteins","score":0.5299999713897705}],"concepts":[{"id":"https://openalex.org/C201815810","wikidata":"https://www.wikidata.org/wiki/Q833066","display_name":"Tethering","level":2,"score":0.6412699222564697},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.625590443611145},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5810841917991638},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.5729668736457825},{"id":"https://openalex.org/C135285700","wikidata":"https://www.wikidata.org/wiki/Q38173","display_name":"G protein-coupled receptor","level":3,"score":0.5310930013656616},{"id":"https://openalex.org/C115537861","wikidata":"https://www.wikidata.org/wiki/Q7645993","display_name":"Surface modification","level":2,"score":0.5271399021148682},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4761536121368408},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.4757290184497833},{"id":"https://openalex.org/C149774189","wikidata":"https://www.wikidata.org/wiki/Q24770541","display_name":"Immunoglobulin domain","level":3,"score":0.4647601842880249},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.45091021060943604},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3522348701953888},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.34908294677734375},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3142196536064148},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.23011791706085205},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.13491597771644592},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.10809031128883362},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1039/d1cb00118c","doi":"https://doi.org/10.1039/d1cb00118c","is_oa":true,"landing_page_url":"https://doi.org/10.1039/d1cb00118c","pdf_url":"https://pubs.rsc.org/en/content/articlepdf/2021/cb/d1cb00118c","source":{"id":"https://openalex.org/S4210222191","display_name":"RSC Chemical Biology","issn_l":"2633-0679","issn":["2633-0679"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","norway-1","oarl","poland-20","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RSC Chemical Biology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8637866","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8637866","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RSC Chem Biol","raw_type":"Text"},{"id":"pmid:34977584","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34977584","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RSC chemical biology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1039/d1cb00118c","doi":"https://doi.org/10.1039/d1cb00118c","is_oa":true,"landing_page_url":"https://doi.org/10.1039/d1cb00118c","pdf_url":"https://pubs.rsc.org/en/content/articlepdf/2021/cb/d1cb00118c","source":{"id":"https://openalex.org/S4210222191","display_name":"RSC Chemical Biology","issn_l":"2633-0679","issn":["2633-0679"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","norway-1","oarl","poland-20","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RSC Chemical Biology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.46810001134872437,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3483810560","display_name":"Sortase-mediated protein engineering for the study of host-pathogen interactions","funder_award_id":"R01AI087879","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G3552353607","display_name":"Hormonal Control of Calcium Metabolism","funder_award_id":"P01DK011794","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G645124401","display_name":"Imposing order on the family of ubiquitin-conjugating (E2) enzymes through intracellular perturbation with nanobodies","funder_award_id":"DP1AI150593","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G9000224774","display_name":null,"funder_award_id":"DK011794","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320306155","display_name":"Cancer Research Institute","ror":"https://ror.org/02f3xk561"},{"id":"https://openalex.org/F4320308349","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3205837077.pdf","grobid_xml":"https://content.openalex.org/works/W3205837077.grobid-xml"},"referenced_works_count":41,"referenced_works":["https://openalex.org/W1988176721","https://openalex.org/W1997730366","https://openalex.org/W1998292458","https://openalex.org/W2028636893","https://openalex.org/W2047687741","https://openalex.org/W2048553889","https://openalex.org/W2079576324","https://openalex.org/W2089788910","https://openalex.org/W2114049739","https://openalex.org/W2114287433","https://openalex.org/W2133572151","https://openalex.org/W2151619201","https://openalex.org/W2156674480","https://openalex.org/W2221707331","https://openalex.org/W2233887337","https://openalex.org/W2342566303","https://openalex.org/W2482833677","https://openalex.org/W2557128364","https://openalex.org/W2597045666","https://openalex.org/W2610778602","https://openalex.org/W2619645085","https://openalex.org/W2766254284","https://openalex.org/W2775795535","https://openalex.org/W2795986766","https://openalex.org/W2799734443","https://openalex.org/W2886130158","https://openalex.org/W2901953282","https://openalex.org/W2919234929","https://openalex.org/W2949064374","https://openalex.org/W2965468477","https://openalex.org/W2972952141","https://openalex.org/W2999524723","https://openalex.org/W3013193077","https://openalex.org/W3022893071","https://openalex.org/W3032049180","https://openalex.org/W3047014325","https://openalex.org/W3081642245","https://openalex.org/W3095931871","https://openalex.org/W3109929953","https://openalex.org/W3126819640","https://openalex.org/W3134299762"],"related_works":["https://openalex.org/W7160879836","https://openalex.org/W7162990290","https://openalex.org/W2911833824","https://openalex.org/W2740185469","https://openalex.org/W4396849605","https://openalex.org/W4396850130","https://openalex.org/W7128779091","https://openalex.org/W7160832252","https://openalex.org/W4210790831","https://openalex.org/W2919234929"],"abstract_inverted_index":{"Antibodies":[0],"raised":[1],"against":[2],"many":[3],"cell":[4,24,37,69],"surface":[5,25,34,70],"proteins,":[6,26],"including":[7],"G":[8],"protein-coupled":[9],"receptors,":[10],"remain":[11],"important":[12],"tools":[13],"for":[14,23,81],"their":[15,84],"functional":[16],"characterization.":[17],"By":[18],"linking":[19],"antibodies":[20,45,60,74,107],"to":[21,32,77,83],"ligands":[22,80],"such":[27],"adducts":[28],"can":[29],"be":[30],"targeted":[31],"the":[33],"of":[35,39,43,86,90,94,112],"a":[36],"type":[38],"choice.":[40],"Site-specific":[41],"functionalization":[42],"full-size":[44],"with":[46,101],"synthetic":[47],"moieties":[48],"remains":[49],"challenging.":[50],"Here":[51],"we":[52],"present":[53],"new":[54],"approaches":[55],"in":[56],"which":[57],"single":[58],"domain":[59],"(known":[61],"as":[62],"VHHs":[63],"or":[64,72],"nanobodies)":[65],"that":[66],"target":[67],"either":[68],"proteins":[71],"conventional":[73],"are":[75],"used":[76],"indirectly":[78],"deliver":[79],"GPCRs":[82],"sites":[85],"action.":[87],"The":[88],"combination":[89],"high":[91],"yield":[92],"production":[93],"nanobodies,":[95],"facile":[96],"site-specific":[97],"functionalization,":[98],"and":[99,105],"compatibility":[100],"commercially":[102],"available":[103],"mouse":[104],"rabbit":[106],"should":[108],"enable":[109],"wide":[110],"application":[111],"this":[113],"approach.":[114]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":1}],"updated_date":"2026-10-29T09:56:32.521200","created_date":"2021-10-25T00:00:00"}