{"id":"https://openalex.org/W3205438579","doi":"https://doi.org/10.3389/fmicb.2021.699235","title":"Metabolic Engineering of Non-carotenoid-Producing Yeast Yarrowia lipolytica for the Biosynthesis of Zeaxanthin","display_name":"Metabolic Engineering of Non-carotenoid-Producing Yeast Yarrowia lipolytica for the Biosynthesis of Zeaxanthin","publication_year":2021,"publication_date":"2021-10-07","ids":{"openalex":"https://openalex.org/W3205438579","doi":"https://doi.org/10.3389/fmicb.2021.699235","mag":"3205438579","pmid":"https://pubmed.ncbi.nlm.nih.gov/34690947","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8529107"},"language":"en","primary_location":{"id":"doi:10.3389/fmicb.2021.699235","doi":"https://doi.org/10.3389/fmicb.2021.699235","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2021.699235","pdf_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2021.699235/pdf","source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2021.699235/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103971527","display_name":"Yuxiao Xie","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yuxiao Xie","raw_affiliation_strings":["Department of Biological Systems Engineering, Washington State University, Pullman, WA, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Systems Engineering, Washington State University, Pullman, WA, United States","institution_ids":["https://openalex.org/I72951846"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038373317","display_name":"Shulin Chen","orcid":"https://orcid.org/0000-0003-0816-2664","observed_orcids":["https://orcid.org/0000-0003-0816-2664"]},"institutions":[{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shulin Chen","raw_affiliation_strings":["Department of Biological Systems Engineering, Washington State University, Pullman, WA, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Systems Engineering, Washington State University, Pullman, WA, United States","institution_ids":["https://openalex.org/I72951846"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5027810259","display_name":"Xiaochao Xiong","orcid":"https://orcid.org/0000-0003-3063-2685","observed_orcids":["https://orcid.org/0000-0003-3063-2685"]},"institutions":[{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Xiaochao Xiong","raw_affiliation_strings":["Department of Biological Systems Engineering, Washington State University, Pullman, WA, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Systems Engineering, Washington State University, Pullman, WA, United States","institution_ids":["https://openalex.org/I72951846"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5027810259"],"corresponding_institution_ids":["https://openalex.org/I72951846"],"apc_list":{"value":2950,"currency":"USD","value_usd":2950},"apc_paid":{"value":2950,"currency":"USD","value_usd":2950},"fwci":1.1884,"has_fulltext":true,"cited_by_count":32,"citation_normalized_percentile":{"value":0.76820487,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":95,"max":99},"biblio":{"volume":"12","issue":null,"first_page":"699235","last_page":"699235"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.7968999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.7968999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.11909999698400497,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12673","display_name":"Microbial Metabolism and Applications","score":0.06840000301599503,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/yarrowia-lipolytica","display_name":"Yarrowia lipolytica","score":0.9980000257492065},{"id":"https://openalex.org/keywords/carotenoid-production","display_name":"carotenoid production","score":0.7710000276565552},{"id":"https://openalex.org/keywords/beta-carotene-biosynthesis","display_name":"\u03b2-carotene biosynthesis","score":0.8220000267028809},{"id":"https://openalex.org/keywords/beta-carotene-hydroxylase","display_name":"\u03b2-carotene hydroxylase","score":0.9539999961853027},{"id":"https://openalex.org/keywords/pantoea-ananatis","display_name":"Pantoea ananatis","score":0.9760000109672546},{"id":"https://openalex.org/keywords/lycopene","display_name":"lycopene","score":0.6949999928474426},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"metabolic engineering","score":0.6589999794960022}],"concepts":[{"id":"https://openalex.org/C2779490540","wikidata":"https://www.wikidata.org/wiki/Q10723443","display_name":"Yarrowia","level":3,"score":0.9403776526451111},{"id":"https://openalex.org/C2776099141","wikidata":"https://www.wikidata.org/wiki/Q169337","display_name":"Zeaxanthin","level":4,"score":0.9349846839904785},{"id":"https://openalex.org/C28781525","wikidata":"https://www.wikidata.org/wiki/Q191907","display_name":"Carotenoid","level":2,"score":0.7325267791748047},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6132444739341736},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6078647971153259},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.5707706212997437},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.5425143837928772},{"id":"https://openalex.org/C2780962900","wikidata":"https://www.wikidata.org/wiki/Q413740","display_name":"Astaxanthin","level":3,"score":0.5054340362548828},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.44632455706596375},{"id":"https://openalex.org/C2776998673","wikidata":"https://www.wikidata.org/wiki/Q190156","display_name":"Carotene","level":2,"score":0.43010926246643066},{"id":"https://openalex.org/C2778411929","wikidata":"https://www.wikidata.org/wiki/Q422067","display_name":"Lutein","level":3,"score":0.42226383090019226},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3242514729499817},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2394210696220398}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3389/fmicb.2021.699235","doi":"https://doi.org/10.3389/fmicb.2021.699235","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2021.699235","pdf_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2021.699235/pdf","source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},{"id":"pmid:34690947","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34690947","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8529107","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8529107/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Microbiol","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:f4608815f65f4dbb91d3a0e537b3aa63","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/f4608815f65f4dbb91d3a0e537b3aa63","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Microbiology, Vol 12 (2021)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fmicb.2021.699235","doi":"https://doi.org/10.3389/fmicb.2021.699235","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2021.699235","pdf_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2021.699235/pdf","source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.40450000762939453,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320309913","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17"},{"id":"https://openalex.org/F4320321613","display_name":"Technische Universit\u00e4t Dresden","ror":"https://ror.org/042aqky30"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3205438579.pdf","grobid_xml":"https://content.openalex.org/works/W3205438579.grobid-xml"},"referenced_works_count":42,"referenced_works":["https://openalex.org/W40033085","https://openalex.org/W1856836325","https://openalex.org/W1880932991","https://openalex.org/W1972272564","https://openalex.org/W2014390233","https://openalex.org/W2058681549","https://openalex.org/W2065603022","https://openalex.org/W2079377150","https://openalex.org/W2091858866","https://openalex.org/W2098327518","https://openalex.org/W2130777296","https://openalex.org/W2145741655","https://openalex.org/W2150363952","https://openalex.org/W2334159352","https://openalex.org/W2346827413","https://openalex.org/W2406707649","https://openalex.org/W2461927112","https://openalex.org/W2551321380","https://openalex.org/W2606736518","https://openalex.org/W2612533264","https://openalex.org/W2625501200","https://openalex.org/W2762949402","https://openalex.org/W2765487566","https://openalex.org/W2791263336","https://openalex.org/W2791931465","https://openalex.org/W2799414564","https://openalex.org/W2886079965","https://openalex.org/W2889216082","https://openalex.org/W2900837785","https://openalex.org/W2901121611","https://openalex.org/W2911678795","https://openalex.org/W2913465470","https://openalex.org/W2945456477","https://openalex.org/W2978947507","https://openalex.org/W2980517903","https://openalex.org/W3016638481","https://openalex.org/W3023190156","https://openalex.org/W3037693826","https://openalex.org/W3044879384","https://openalex.org/W3047419816","https://openalex.org/W3049102029","https://openalex.org/W3160732670"],"related_works":["https://openalex.org/W4386501755","https://openalex.org/W4404058969","https://openalex.org/W4292261525","https://openalex.org/W2765487566","https://openalex.org/W3008999672","https://openalex.org/W7112494113","https://openalex.org/W2762949402","https://openalex.org/W4391912129","https://openalex.org/W4408031530","https://openalex.org/W3154824894"],"abstract_inverted_index":{"Zeaxanthin":[0],"is":[1,15,34],"vital":[2],"to":[3,52,116,173,232,286],"human":[4],"health;":[5],"thus,":[6],"its":[7],"production":[8,62],"has":[9,44,66,78],"received":[10],"much":[11],"attention,":[12],"and":[13,30,43,58,97,143,182,267,281],"it":[14],"also":[16],"an":[17,284],"essential":[18],"precursor":[19],"for":[20,120,159,278,290],"the":[21,37,86,128,139,146,155,162,166,178,187,201,214,234,245,248,252,257,272,291],"biosynthesis":[22,76,280],"of":[23,36,88,125,148,157,169,180,189,217,239,247,254,265,274,293],"other":[24],"critical":[25],"carotenoids":[26,54],"such":[27,55],"as":[28,48,56],"astaxanthin":[29],"crocetin.":[31],"Yarrowia":[32],"lipolytica":[33,65,115,171],"one":[35],"most":[38],"intensively":[39],"studied":[40],"non-conventional":[41],"yeasts":[42],"been":[45,68,79],"genetically":[46],"engineered":[47],"a":[49,174,183,205,225,262,275],"cell":[50,150,276],"factory":[51,277],"produce":[53],"lycopene":[57,266],"\u03b2-carotene.":[59,268],"However,":[60],"zeaxanthin":[61,141,181,193,279],"by":[63,85,200],"Y.":[64,114,170],"not":[67],"well":[69],"investigated.":[70],"To":[71],"fill":[72],"this":[73,83,288],"gap,":[74],"\u03b2-carotene":[75,104],"pathway":[77],"first":[80],"constructed":[81],"in":[82,138,177,186,244],"study":[84],"expression":[87,124],"genes,":[89],"including":[90],"crtE":[91],",":[92,94,96],"crtB":[93],"crtI":[95],"carRP":[98],".":[99],"Three":[100],"crtZ":[101,126],"genes":[102],"encoding":[103],"hydroxylase":[105],"from":[106,127],"different":[107],"organisms":[108],"were":[109],"individually":[110],"introduced":[111],"into":[112,165],"\u03b2-carotene-producing":[113],"evaluate":[117],"their":[118],"performance":[119],"producing":[121,160],"zeaxanthin.":[122,190],"The":[123,191,269],"bacterium":[129],"Pantoea":[130],"ananatis":[131],"(formerly":[132],"Erwinia":[133],"uredovora,":[134],"Eu-crtZ":[135,158],")":[136],"resulted":[137],"highest":[140,192,215],"titer":[142,179,194],"content":[144,188,216],"on":[145,204],"basis":[147],"dry":[149],"weight":[151],"(DCW).":[152],"After":[153],"verifying":[154],"function":[156],"zeaxanthin,":[161],"high-copy-number":[163],"integration":[164],"ribosomal":[167],"DNA":[168],"led":[172],"4.02-fold":[175],"increase":[176,185],"721%":[184],"achieved":[195],"21.98":[196],"\u00b1":[197,221],"1.80":[198],"mg/L":[199],"strain":[202],"grown":[203],"yeast":[206,227],"extract":[207],"peptone":[208],"dextrose":[209],"(YPD)\u2013rich":[210],"medium.":[211],"In":[212],"contrast,":[213],"DCW":[218],"reached":[219],"3.20":[220],"0.11":[222],"mg/g":[223],"using":[224],"synthetic":[226],"nitrogen":[228],"base":[229],"(YNB)":[230],"medium":[231,250],"culture":[233],"cells.":[235],"Over":[236],"18.0":[237],"g/L":[238],"citric":[240],"acid":[241],"was":[242],"detected":[243],"supernatant":[246],"YPD":[249],"at":[251],"end":[253],"cultivation.":[255],"Furthermore,":[256],"zeaxanthin-producing":[258],"strains":[259],"still":[260],"accumulated":[261],"large":[263],"amount":[264],"results":[270],"demonstrated":[271],"potential":[273],"opened":[282],"up":[283],"avenue":[285],"engineer":[287],"host":[289],"overproduction":[292],"carotenoids.":[294]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":10},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":11},{"year":2022,"cited_by_count":3}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2021-10-25T00:00:00"}