{"id":"https://openalex.org/W3203729472","doi":"https://doi.org/10.1016/j.sbi.2021.07.010","title":"High throughput and quantitative enzymology in the genomic era","display_name":"High throughput and quantitative enzymology in the genomic era","publication_year":2021,"publication_date":"2021-09-27","ids":{"openalex":"https://openalex.org/W3203729472","doi":"https://doi.org/10.1016/j.sbi.2021.07.010","mag":"3203729472","pmid":"https://pubmed.ncbi.nlm.nih.gov/34592682","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8648990"},"language":"en","primary_location":{"id":"doi:10.1016/j.sbi.2021.07.010","doi":"https://doi.org/10.1016/j.sbi.2021.07.010","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.sbi.2021.07.010","pdf_url":null,"source":{"id":"https://openalex.org/S175155502","display_name":"Current Opinion in Structural Biology","issn_l":"0959-440X","issn":["0959-440X","1879-033X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Current Opinion in Structural Biology","raw_type":"journal-article"},"volume":null,"type":"review","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://doi.org/10.1016/j.sbi.2021.07.010","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079499900","display_name":"Daniel Alexander Mokhtari","orcid":"https://orcid.org/0000-0003-4206-9471","observed_orcids":["https://orcid.org/0000-0003-4206-9471"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D.A. Mokhtari","raw_affiliation_strings":["Department of Biochemistry, Stanford University, Stanford, CA, 94305, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Stanford University, Stanford, CA, 94305, USA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110865446","display_name":"Mason J. Appel","orcid":"https://orcid.org/0000-0001-7501-8115","observed_orcids":["https://orcid.org/0000-0001-7501-8115"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M.J. Appel","raw_affiliation_strings":["Department of Biochemistry, Stanford University, Stanford, CA, 94305, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Stanford University, Stanford, CA, 94305, USA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085863617","display_name":"Polly Morrell Fordyce","orcid":"https://orcid.org/0000-0002-9505-0638","observed_orcids":["https://orcid.org/0000-0002-9505-0638"]},"institutions":[{"id":"https://openalex.org/I4210100286","display_name":"Chan Zuckerberg Biohub San Francisco","ror":"https://ror.org/00knt4f32","country_code":"US","type":"facility","lineage":["https://openalex.org/I180670191","https://openalex.org/I4210100286","https://openalex.org/I4210121800","https://openalex.org/I95457486","https://openalex.org/I97018004"]},{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"P.M. Fordyce","raw_affiliation_strings":["Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA; ChEM-H Institute, Stanford University, Stanford, CA, 94305, USA; Department of Genetics, Stanford University, Stanford, CA, 94305, USA; Chan Zuckerberg Biohub San Francisco, CA, 94110, USA. Electronic address: pfordyce@stanford.edu","ChEM-H Institute, Stanford University, Stanford, CA, 94305, USA","Chan Zuckerberg Biohub San Francisco, CA, 94110, USA","Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA","Department of Genetics, Stanford University, Stanford, CA, 94305, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA; ChEM-H Institute, Stanford University, Stanford, CA, 94305, USA; Department of Genetics, Stanford University, Stanford, CA, 94305, USA; Chan Zuckerberg Biohub San Francisco, CA, 94110, USA. Electronic address: pfordyce@stanford.edu","institution_ids":["https://openalex.org/I4210100286","https://openalex.org/I97018004"]},{"raw_affiliation_string":"ChEM-H Institute, Stanford University, Stanford, CA, 94305, USA","institution_ids":["https://openalex.org/I97018004"]},{"raw_affiliation_string":"Chan Zuckerberg Biohub San Francisco, CA, 94110, USA","institution_ids":["https://openalex.org/I4210100286"]},{"raw_affiliation_string":"Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA","institution_ids":["https://openalex.org/I97018004"]},{"raw_affiliation_string":"Department of Genetics, Stanford University, Stanford, CA, 94305, USA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5076625801","display_name":"Daniel Herschlag","orcid":"https://orcid.org/0000-0002-4685-1973","observed_orcids":["https://orcid.org/0000-0002-4685-1973"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"D. Herschlag","raw_affiliation_strings":["Department of Biochemistry, Stanford University, Stanford, CA, 94305, USA; Department of Chemical Engineering, Stanford University, Stanford, CA, 94305, USA; ChEM-H Institute, Stanford University, Stanford, CA, 94305, USA. Electronic address: herschla@stanford.edu","ChEM-H Institute, Stanford University, Stanford, CA, 94305, USA","Department of Biochemistry, Stanford University, Stanford, CA, 94305, USA","Department of Chemical Engineering, Stanford University, Stanford, CA, 94305, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Stanford University, Stanford, CA, 94305, USA; Department of Chemical Engineering, Stanford University, Stanford, CA, 94305, USA; ChEM-H Institute, Stanford University, Stanford, CA, 94305, USA. Electronic address: herschla@stanford.edu","institution_ids":["https://openalex.org/I97018004"]},{"raw_affiliation_string":"ChEM-H Institute, Stanford University, Stanford, CA, 94305, USA","institution_ids":["https://openalex.org/I97018004"]},{"raw_affiliation_string":"Department of Biochemistry, Stanford University, Stanford, CA, 94305, USA","institution_ids":["https://openalex.org/I97018004"]},{"raw_affiliation_string":"Department of Chemical Engineering, Stanford University, Stanford, CA, 94305, USA","institution_ids":["https://openalex.org/I97018004"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5076625801","https://openalex.org/A5085863617"],"corresponding_institution_ids":["https://openalex.org/I4210100286","https://openalex.org/I97018004"],"apc_list":{"value":4400,"currency":"USD","value_usd":4400},"apc_paid":null,"fwci":0.5063,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.45148331,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":96,"max":99},"biblio":{"volume":"71","issue":null,"first_page":"259","last_page":"273"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.6161999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.6161999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.3411000072956085,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T13539","display_name":"thermodynamics and calorimetric analyses","score":0.014100000262260437,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.7990000247955322},{"id":"https://openalex.org/keywords/high-throughput-enzyme-assay","display_name":"high-throughput enzyme assay","score":0.7670000195503235},{"id":"https://openalex.org/keywords/enzyme-mechanism","display_name":"enzyme mechanism","score":0.5049999952316284},{"id":"https://openalex.org/keywords/enzyme-structure-function-relationship","display_name":"enzyme structure-function","score":0.5889999866485596},{"id":"https://openalex.org/keywords/rate-constants","display_name":"rate constants","score":0.39399999380111694}],"concepts":[{"id":"https://openalex.org/C146849305","wikidata":"https://www.wikidata.org/wiki/Q370766","display_name":"Ground truth","level":2,"score":0.7041080594062805},{"id":"https://openalex.org/C176217482","wikidata":"https://www.wikidata.org/wiki/Q860554","display_name":"Metric (unit)","level":2,"score":0.6423965692520142},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5942801833152771},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.5739741325378418},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.39364200830459595},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.37248313426971436},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.2937169671058655},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.21896997094154358},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.09242895245552063},{"id":"https://openalex.org/C21547014","wikidata":"https://www.wikidata.org/wiki/Q1423657","display_name":"Operations management","level":1,"score":0.0},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001703","descriptor_name":"Biophysics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D055592","descriptor_name":"Biophysical Phenomena","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.sbi.2021.07.010","doi":"https://doi.org/10.1016/j.sbi.2021.07.010","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.sbi.2021.07.010","pdf_url":null,"source":{"id":"https://openalex.org/S175155502","display_name":"Current Opinion in Structural Biology","issn_l":"0959-440X","issn":["0959-440X","1879-033X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Current Opinion in Structural Biology","raw_type":"journal-article"},{"id":"pmid:34592682","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34592682","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current opinion in structural biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8648990","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8648990","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Curr Opin Struct Biol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1016/j.sbi.2021.07.010","doi":"https://doi.org/10.1016/j.sbi.2021.07.010","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.sbi.2021.07.010","pdf_url":null,"source":{"id":"https://openalex.org/S175155502","display_name":"Current Opinion in Structural Biology","issn_l":"0959-440X","issn":["0959-440X","1879-033X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Current Opinion in Structural Biology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G9000879693","display_name":"Quantitative, High-throughput Mechanistic Enzymology","funder_award_id":"R01GM064798","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":120,"referenced_works":["https://openalex.org/W360939430","https://openalex.org/W598971431","https://openalex.org/W1493541064","https://openalex.org/W1526446139","https://openalex.org/W1632044512","https://openalex.org/W1837057750","https://openalex.org/W1965093499","https://openalex.org/W1967582753","https://openalex.org/W1972346855","https://openalex.org/W1974093948","https://openalex.org/W1978159078","https://openalex.org/W1984527736","https://openalex.org/W1984965754","https://openalex.org/W1987696643","https://openalex.org/W1987953658","https://openalex.org/W1998785678","https://openalex.org/W2000293919","https://openalex.org/W2002219567","https://openalex.org/W2013425283","https://openalex.org/W2013726100","https://openalex.org/W2018093699","https://openalex.org/W2026513163","https://openalex.org/W2035100713","https://openalex.org/W2038282326","https://openalex.org/W2041205020","https://openalex.org/W2047851714","https://openalex.org/W2049576685","https://openalex.org/W2049934777","https://openalex.org/W2054191418","https://openalex.org/W2055808883","https://openalex.org/W2059997198","https://openalex.org/W2060588922","https://openalex.org/W2061042699","https://openalex.org/W2062237921","https://openalex.org/W2072660673","https://openalex.org/W2086951662","https://openalex.org/W2087126377","https://openalex.org/W2087432921","https://openalex.org/W2091748192","https://openalex.org/W2094795801","https://openalex.org/W2102066010","https://openalex.org/W2105406676","https://openalex.org/W2107609376","https://openalex.org/W2108116455","https://openalex.org/W2109801072","https://openalex.org/W2112499570","https://openalex.org/W2112636022","https://openalex.org/W2112907786","https://openalex.org/W2119246087","https://openalex.org/W2119273654","https://openalex.org/W2125096693","https://openalex.org/W2130479394","https://openalex.org/W2142962510","https://openalex.org/W2146030191","https://openalex.org/W2146134187","https://openalex.org/W2151100442","https://openalex.org/W2158700188","https://openalex.org/W2159985140","https://openalex.org/W2160903751","https://openalex.org/W2162943526","https://openalex.org/W2163740345","https://openalex.org/W2164208203","https://openalex.org/W2194144568","https://openalex.org/W2239008737","https://openalex.org/W2265800909","https://openalex.org/W2278720940","https://openalex.org/W2313577593","https://openalex.org/W2315174892","https://openalex.org/W2325324490","https://openalex.org/W2380593613","https://openalex.org/W2463220226","https://openalex.org/W2470892237","https://openalex.org/W2471654536","https://openalex.org/W2525394342","https://openalex.org/W2556032506","https://openalex.org/W2564162774","https://openalex.org/W2606362592","https://openalex.org/W2606439133","https://openalex.org/W2609912642","https://openalex.org/W2619833189","https://openalex.org/W2621926380","https://openalex.org/W2623556860","https://openalex.org/W2727251566","https://openalex.org/W2740096108","https://openalex.org/W2754649780","https://openalex.org/W2766457884","https://openalex.org/W2767078712","https://openalex.org/W2773276133","https://openalex.org/W2806783543","https://openalex.org/W2849619728","https://openalex.org/W2908318836","https://openalex.org/W2909711988","https://openalex.org/W2947786247","https://openalex.org/W2950343954","https://openalex.org/W2952317511","https://openalex.org/W2963734385","https://openalex.org/W2968494487","https://openalex.org/W2975848503","https://openalex.org/W2980610099","https://openalex.org/W2987965949","https://openalex.org/W2989608901","https://openalex.org/W2991366853","https://openalex.org/W2999044305","https://openalex.org/W3023074808","https://openalex.org/W3032758350","https://openalex.org/W3044778276","https://openalex.org/W3044941661","https://openalex.org/W3046468150","https://openalex.org/W3088927464","https://openalex.org/W3091857140","https://openalex.org/W3096624764","https://openalex.org/W3106395629","https://openalex.org/W3108171657","https://openalex.org/W3111135617","https://openalex.org/W3128777856","https://openalex.org/W3135682489","https://openalex.org/W3140562424","https://openalex.org/W3171407157","https://openalex.org/W3188536111","https://openalex.org/W4230546834"],"related_works":["https://openalex.org/W4409002246","https://openalex.org/W4251974256","https://openalex.org/W4388903750","https://openalex.org/W7154132720","https://openalex.org/W2912490461","https://openalex.org/W2907138185","https://openalex.org/W1972309823","https://openalex.org/W2311960866","https://openalex.org/W2909856037","https://openalex.org/W4393052547"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":8},{"year":2022,"cited_by_count":5}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2021-10-11T00:00:00"}