{"id":"https://openalex.org/W3203078633","doi":"https://doi.org/10.1038/s41598-021-99389-4","title":"Applying PICRUSt and 16S rRNA functional characterisation to predicting co-digestion strategies of various animal manures for biogas production","display_name":"Applying PICRUSt and 16S rRNA functional characterisation to predicting co-digestion strategies of various animal manures for biogas production","publication_year":2021,"publication_date":"2021-10-07","ids":{"openalex":"https://openalex.org/W3203078633","doi":"https://doi.org/10.1038/s41598-021-99389-4","mag":"3203078633","pmid":"https://pubmed.ncbi.nlm.nih.gov/34620937"},"language":"en","primary_location":{"id":"doi:10.1038/s41598-021-99389-4","doi":"https://doi.org/10.1038/s41598-021-99389-4","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-021-99389-4","pdf_url":"https://www.nature.com/articles/s41598-021-99389-4.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/s41598-021-99389-4.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051595229","display_name":"Grace N. Ijoma","orcid":"https://orcid.org/0000-0002-1568-8851","observed_orcids":["https://orcid.org/0000-0002-1568-8851"]},"institutions":[{"id":"https://openalex.org/I165390105","display_name":"University of South Africa","ror":"https://ror.org/048cwvf49","country_code":"ZA","type":"education","lineage":["https://openalex.org/I165390105"]}],"countries":["ZA"],"is_corresponding":true,"raw_author_name":"Grace N. Ijoma","raw_affiliation_strings":["Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa. nkechiijoma@gmail.com","Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa"],"raw_orcid":"https://orcid.org/0000-0002-1568-8851","affiliations":[{"raw_affiliation_string":"Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa. nkechiijoma@gmail.com","institution_ids":["https://openalex.org/I165390105"]},{"raw_affiliation_string":"Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa","institution_ids":["https://openalex.org/I165390105"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091645914","display_name":"Rosina Nkuna","orcid":"https://orcid.org/0000-0002-1125-2989","observed_orcids":["https://orcid.org/0000-0002-1125-2989"]},"institutions":[{"id":"https://openalex.org/I165390105","display_name":"University of South Africa","ror":"https://ror.org/048cwvf49","country_code":"ZA","type":"education","lineage":["https://openalex.org/I165390105"]}],"countries":["ZA"],"is_corresponding":false,"raw_author_name":"Rosina Nkuna","raw_affiliation_strings":["Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa"],"raw_orcid":"https://orcid.org/0000-0002-1125-2989","affiliations":[{"raw_affiliation_string":"Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa","institution_ids":["https://openalex.org/I165390105"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038962258","display_name":"Asheal Mutungwazi","orcid":"https://orcid.org/0000-0001-9808-0261","observed_orcids":["https://orcid.org/0000-0001-9808-0261"]},"institutions":[{"id":"https://openalex.org/I165390105","display_name":"University of South Africa","ror":"https://ror.org/048cwvf49","country_code":"ZA","type":"education","lineage":["https://openalex.org/I165390105"]}],"countries":["ZA"],"is_corresponding":false,"raw_author_name":"Asheal Mutungwazi","raw_affiliation_strings":["Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa"],"raw_orcid":"https://orcid.org/0000-0001-9808-0261","affiliations":[{"raw_affiliation_string":"Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa","institution_ids":["https://openalex.org/I165390105"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022473429","display_name":"Charles Rashama","orcid":"https://orcid.org/0000-0002-2149-755X","observed_orcids":["https://orcid.org/0000-0002-2149-755X"]},"institutions":[{"id":"https://openalex.org/I165390105","display_name":"University of South Africa","ror":"https://ror.org/048cwvf49","country_code":"ZA","type":"education","lineage":["https://openalex.org/I165390105"]}],"countries":["ZA"],"is_corresponding":false,"raw_author_name":"Charles Rashama","raw_affiliation_strings":["Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa"],"raw_orcid":"https://orcid.org/0000-0002-2149-755X","affiliations":[{"raw_affiliation_string":"Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa","institution_ids":["https://openalex.org/I165390105"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5060246107","display_name":"Tonderayi Sylvester Matambo","orcid":"https://orcid.org/0000-0001-9432-3772","observed_orcids":["https://orcid.org/0000-0001-9432-3772"]},"institutions":[{"id":"https://openalex.org/I165390105","display_name":"University of South Africa","ror":"https://ror.org/048cwvf49","country_code":"ZA","type":"education","lineage":["https://openalex.org/I165390105"]}],"countries":["ZA"],"is_corresponding":false,"raw_author_name":"Tonderayi S. Matambo","raw_affiliation_strings":["Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa"],"raw_orcid":"https://orcid.org/0000-0001-9432-3772","affiliations":[{"raw_affiliation_string":"Institute for the Development of Energy for African Sustainability, University of South Africa, Roodepoort, 1709, South Africa","institution_ids":["https://openalex.org/I165390105"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5051595229"],"corresponding_institution_ids":["https://openalex.org/I165390105"],"apc_list":{"value":1990,"currency":"USD","value_usd":1990},"apc_paid":{"value":1990,"currency":"USD","value_usd":1990},"fwci":3.8122,"has_fulltext":true,"cited_by_count":43,"citation_normalized_percentile":{"value":0.93507981,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":96,"max":99},"biblio":{"volume":"11","issue":"1","first_page":"19913","last_page":"19913"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10284","display_name":"Anaerobic Digestion and Biogas Production","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2215","display_name":"Building and Construction"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10284","display_name":"Anaerobic Digestion and Biogas Production","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2215","display_name":"Building and Construction"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11231","display_name":"Microbial Fuel Cells and Bioremediation","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2305","display_name":"Environmental Engineering"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11275","display_name":"Composting and Vermicomposting Techniques","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1111","display_name":"Soil Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/anaerobic-digestion","display_name":"anaerobic digestion","score":0.7879999876022339},{"id":"https://openalex.org/keywords/co-digestion","display_name":"co-digestion","score":0.9399999976158142},{"id":"https://openalex.org/keywords/livestock-manure","display_name":"livestock manure","score":0.9810000061988831},{"id":"https://openalex.org/keywords/biogas-production","display_name":"biogas production","score":0.9330000281333923},{"id":"https://openalex.org/keywords/picrust","display_name":"PICRUSt","score":0.7680000066757202},{"id":"https://openalex.org/keywords/16s-rrna","display_name":"16S rRNA","score":0.847000002861023},{"id":"https://openalex.org/keywords/kegg-orthology","display_name":"KEGG Orthology","score":0.722000002861023},{"id":"https://openalex.org/keywords/horse-manure","display_name":"horse manure","score":0.9490000009536743},{"id":"https://openalex.org/keywords/cattle-manure","display_name":"cattle manure","score":0.9909999966621399},{"id":"https://openalex.org/keywords/pig-manure","display_name":"pig manure","score":1.0},{"id":"https://openalex.org/keywords/biochemical-methane-potential","display_name":"biochemical methane potential","score":0.8539999723434448},{"id":"https://openalex.org/keywords/acidogenesis","display_name":"acidogenesis","score":0.5609999895095825},{"id":"https://openalex.org/keywords/acetogenesis","display_name":"acetogenesis","score":0.9890000224113464},{"id":"https://openalex.org/keywords/carbohydrate-metabolism","display_name":"carbohydrate metabolism","score":0.46799999475479126},{"id":"https://openalex.org/keywords/phosphotransferase","display_name":"phosphotransferase","score":0.8240000009536743}],"concepts":[{"id":"https://openalex.org/C499616599","wikidata":"https://www.wikidata.org/wiki/Q555853","display_name":"Anaerobic digestion","level":3,"score":0.6988031268119812},{"id":"https://openalex.org/C2781357212","wikidata":"https://www.wikidata.org/wiki/Q5275608","display_name":"Digestion (alchemy)","level":2,"score":0.6948543787002563},{"id":"https://openalex.org/C75212476","wikidata":"https://www.wikidata.org/wiki/Q171076","display_name":"Biogas","level":2,"score":0.645923376083374},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5841400623321533},{"id":"https://openalex.org/C53247381","wikidata":"https://www.wikidata.org/wiki/Q21119664","display_name":"Acidogenesis","level":4,"score":0.5782604813575745},{"id":"https://openalex.org/C38304854","wikidata":"https://www.wikidata.org/wiki/Q185716","display_name":"Manure","level":2,"score":0.5752881169319153},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.49447202682495117},{"id":"https://openalex.org/C2778713356","wikidata":"https://www.wikidata.org/wiki/Q2919619","display_name":"Fructokinase","level":3,"score":0.4328210651874542},{"id":"https://openalex.org/C529335014","wikidata":"https://www.wikidata.org/wiki/Q41534","display_name":"Starch","level":2,"score":0.43116405606269836},{"id":"https://openalex.org/C2776970464","wikidata":"https://www.wikidata.org/wiki/Q122043","display_name":"Fructose","level":2,"score":0.4173041880130768},{"id":"https://openalex.org/C2341566","wikidata":"https://www.wikidata.org/wiki/Q910759","display_name":"Cow dung","level":3,"score":0.41679179668426514},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3954036235809326},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.36983969807624817},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2848568558692932},{"id":"https://openalex.org/C6557445","wikidata":"https://www.wikidata.org/wiki/Q173113","display_name":"Agronomy","level":1,"score":0.2279679775238037},{"id":"https://openalex.org/C2780560099","wikidata":"https://www.wikidata.org/wiki/Q83323","display_name":"Fertilizer","level":2,"score":0.12934935092926025},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.10565903782844543},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.08472272753715515},{"id":"https://openalex.org/C516920438","wikidata":"https://www.wikidata.org/wiki/Q37129","display_name":"Methane","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000693","descriptor_name":"Anaerobiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000693","descriptor_name":"Anaerobiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000693","descriptor_name":"Anaerobiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000821","descriptor_name":"Animal Feed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000821","descriptor_name":"Animal Feed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000821","descriptor_name":"Animal Feed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005285","descriptor_name":"Fermentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005285","descriptor_name":"Fermentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005285","descriptor_name":"Fermentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008372","descriptor_name":"Manure","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D008372","descriptor_name":"Manure","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D008372","descriptor_name":"Manure","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D008697","descriptor_name":"Methane","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D008697","descriptor_name":"Methane","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D008697","descriptor_name":"Methane","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D054892","descriptor_name":"Metagenome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054892","descriptor_name":"Metagenome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054892","descriptor_name":"Metagenome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056186","descriptor_name":"Metagenomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D056186","descriptor_name":"Metagenomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D056186","descriptor_name":"Metagenomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D056804","descriptor_name":"Biofuels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D056804","descriptor_name":"Biofuels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D056804","descriptor_name":"Biofuels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1038/s41598-021-99389-4","doi":"https://doi.org/10.1038/s41598-021-99389-4","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-021-99389-4","pdf_url":"https://www.nature.com/articles/s41598-021-99389-4.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"pmid:34620937","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34620937","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8497515","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8497515","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Sci Rep","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:3a95c45181e6486ab69c999733ed194d","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/3a95c45181e6486ab69c999733ed194d","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1038/s41598-021-99389-4","doi":"https://doi.org/10.1038/s41598-021-99389-4","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-021-99389-4","pdf_url":"https://www.nature.com/articles/s41598-021-99389-4.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.5666999816894531,"id":"https://metadata.un.org/sdg/2"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1509130374","display_name":null,"funder_award_id":"DST/CON0197/2017","funder_id":"https://openalex.org/F4320317010","funder_display_name":"Department of Science and Innovation, South Africa"}],"funders":[{"id":"https://openalex.org/F4320317010","display_name":"Department of Science and Innovation, South Africa","ror":null},{"id":"https://openalex.org/F4320320719","display_name":"Department of Science and Technology, Ministry of Science and Technology, India","ror":"https://ror.org/0101xrq71"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3203078633.pdf","grobid_xml":"https://content.openalex.org/works/W3203078633.grobid-xml"},"referenced_works_count":131,"referenced_works":["https://openalex.org/W761500788","https://openalex.org/W1544361270","https://openalex.org/W1603491745","https://openalex.org/W1606288493","https://openalex.org/W1773421998","https://openalex.org/W1842963151","https://openalex.org/W1856219842","https://openalex.org/W1859870252","https://openalex.org/W1887356965","https://openalex.org/W1909727327","https://openalex.org/W1952647799","https://openalex.org/W1963531361","https://openalex.org/W1967966999","https://openalex.org/W1978046919","https://openalex.org/W1978381467","https://openalex.org/W1983636509","https://openalex.org/W1991892080","https://openalex.org/W2000848198","https://openalex.org/W2022023730","https://openalex.org/W2025621225","https://openalex.org/W2026902894","https://openalex.org/W2031117980","https://openalex.org/W2040112902","https://openalex.org/W2041644889","https://openalex.org/W2046458324","https://openalex.org/W2057540875","https://openalex.org/W2062223074","https://openalex.org/W2063782289","https://openalex.org/W2065055809","https://openalex.org/W2089442209","https://openalex.org/W2097634793","https://openalex.org/W2099665447","https://openalex.org/W2104435982","https://openalex.org/W2105876737","https://openalex.org/W2106492499","https://openalex.org/W2110300022","https://openalex.org/W2117106990","https://openalex.org/W2118217258","https://openalex.org/W2120252476","https://openalex.org/W2129029433","https://openalex.org/W2129992387","https://openalex.org/W2135646174","https://openalex.org/W2145132019","https://openalex.org/W2148749381","https://openalex.org/W2159010530","https://openalex.org/W2159482845","https://openalex.org/W2219309259","https://openalex.org/W2262287101","https://openalex.org/W2289442482","https://openalex.org/W2291011464","https://openalex.org/W2332064818","https://openalex.org/W2334372952","https://openalex.org/W2401905738","https://openalex.org/W2416595113","https://openalex.org/W2427290044","https://openalex.org/W2496834145","https://openalex.org/W2509051298","https://openalex.org/W2510540571","https://openalex.org/W2518527147","https://openalex.org/W2529356329","https://openalex.org/W2580767461","https://openalex.org/W2588214264","https://openalex.org/W2593046558","https://openalex.org/W2611297635","https://openalex.org/W2612872172","https://openalex.org/W2620630126","https://openalex.org/W2737654008","https://openalex.org/W2739246990","https://openalex.org/W2758035336","https://openalex.org/W2768597717","https://openalex.org/W2777103518","https://openalex.org/W2778425787","https://openalex.org/W2779680122","https://openalex.org/W2783242163","https://openalex.org/W2797564545","https://openalex.org/W2799451234","https://openalex.org/W2883805016","https://openalex.org/W2884829691","https://openalex.org/W2888926155","https://openalex.org/W2890031062","https://openalex.org/W2893388127","https://openalex.org/W2896983921","https://openalex.org/W2897574871","https://openalex.org/W2901294656","https://openalex.org/W2901645227","https://openalex.org/W2901686903","https://openalex.org/W2905300225","https://openalex.org/W2905498624","https://openalex.org/W2910571685","https://openalex.org/W2912973122","https://openalex.org/W2914484401","https://openalex.org/W2920025494","https://openalex.org/W2925327687","https://openalex.org/W2944535522","https://openalex.org/W2945050756","https://openalex.org/W2954442150","https://openalex.org/W2954979592","https://openalex.org/W2969718750","https://openalex.org/W2974418630","https://openalex.org/W2980507114","https://openalex.org/W2992240681","https://openalex.org/W2997057391","https://openalex.org/W3003044952","https://openalex.org/W3004227146","https://openalex.org/W3006610610","https://openalex.org/W3013068412","https://openalex.org/W3015461485","https://openalex.org/W3018002314","https://openalex.org/W3018177943","https://openalex.org/W3018901688","https://openalex.org/W3034616104","https://openalex.org/W3038172987","https://openalex.org/W3041840808","https://openalex.org/W3044286336","https://openalex.org/W3044747673","https://openalex.org/W3087947947","https://openalex.org/W3091952020","https://openalex.org/W3093052378","https://openalex.org/W3095394567","https://openalex.org/W3098673665","https://openalex.org/W3107163060","https://openalex.org/W3111380248","https://openalex.org/W3111726075","https://openalex.org/W3118018179","https://openalex.org/W3127302140","https://openalex.org/W3133493442","https://openalex.org/W3196025804","https://openalex.org/W4231099889","https://openalex.org/W4234938677","https://openalex.org/W4246406171","https://openalex.org/W4294216483"],"related_works":["https://openalex.org/W2946132100","https://openalex.org/W2034639174","https://openalex.org/W1555150184","https://openalex.org/W3017104984","https://openalex.org/W2009435281","https://openalex.org/W2083679481","https://openalex.org/W1981885716","https://openalex.org/W2793202822","https://openalex.org/W1991593228","https://openalex.org/W4235134868"],"abstract_inverted_index":{"An":[0],"estimated":[1],"25":[2],"million":[3],"tons":[4],"of":[5,27,41,75,105,109,115,126,136,192,247],"animal":[6],"manure":[7,189,249],"is":[8,45,272],"produced":[9],"globally":[10],"every":[11],"year,":[12],"causing":[13],"considerable":[14],"impact":[15],"to":[16,48,63,71,97,197],"the":[17,25,39,42,59,73,98,113,123,153,161,193,198,230,243,248,254,258],"environment.":[18],"These":[19],"impacts":[20],"can":[21,233,265],"be":[22,234,266],"managed":[23],"through":[24,36,236],"use":[26],"anaerobic":[28],"digestion":[29],"(AD)":[30],"This":[31],"process":[32,44,232],"achieves":[33],"waste":[34],"degradation":[35],"enzymatic":[37],"activity,":[38],"efficiency":[40],"AD":[43,202,231],"directly":[46],"related":[47,196],"microorganisms":[49],"that":[50,87,167,208,239],"produce":[51],"these":[52,228],"enzymes.":[53],"Biomethane":[54],"potential":[55],"(BMP)":[56],"assays":[57,116],"remain":[58],"standard":[60],"theoretical":[61],"framework":[62],"pre-determine":[64],"biogas":[65,81],"yield":[66],"and":[67,91,129,148,172,181,222],"have":[68],"been":[69],"used":[70],"determine":[72],"feasibility":[74],"substrates":[76],"or":[77],"their":[78],"combination":[79],"for":[80,214,220,223,257],"production.":[82],"However,":[83],"an":[84,95],"integrated":[85],"approach":[86,271],"combines":[88],"substrate":[89],"choice":[90],"co-digestion":[92,237],"would":[93],"provide":[94],"improvement":[96],"current":[99],"predictive":[100],"models.":[101],"PICRUSt":[102],"(Phylogenetic":[103],"Investigation":[104],"Communities":[106],"by":[107],"Reconstruction":[108],"Unobserved":[110],"States)":[111],"addresses":[112],"limitations":[114],"in":[117,151,187,260,274],"this":[118,121,261,269],"regard.":[119],"In":[120],"paper,":[122],"biochemical":[124],"functions":[125],"horse,":[127],"cow,":[128],"pig":[130],"manures":[131],"are":[132],"predicted.":[133],"A":[134],"total":[135],"135":[137],"predicted":[138,219,244],"KEGG":[139],"Orthologies":[140],"(KOs)":[141],"showed":[142,166,207],"amino":[143,170],"acids,":[144],"carbohydrate,":[145],"energy,":[146],"lipid,":[147],"xenobiotic":[149],"metabolisms":[150,183],"all":[152],"samples.":[154,190,250],"Linear":[155],"discriminant":[156],"analysis":[157,276],"(LDA)":[158],"combined":[159,270],"with":[160],"effect":[162],"size":[163],"measurements":[164],"(LEfSe),":[165],"fructose,":[168],"mannose,":[169],"acid":[171],"nucleotide":[173],"sugar,":[174],"phosphotransferase":[175],"(PST)":[176],"as":[177,179],"well":[178],"starch":[180],"sucrose":[182],"were":[184,195,212,218],"significantly":[185],"higher":[186],"horse":[188],"36":[191],"KOs":[194],"acidogenesis":[199],"and/or":[200],"acetogenesis":[201],"stages.":[203],"Extended":[204],"bar":[205],"plots":[206],"11":[209],"significant":[210],"predictions":[211],"observed":[213],"horse-cow,":[215],"while":[216],"5":[217],"horse-pig":[221],"cow-pig":[224],"manures.":[225],"Based":[226],"on":[227],"predictions,":[229],"enhanced":[235],"strategies":[238],"takes":[240],"into":[241],"account":[242],"metabolic":[245],"contributions":[246],"The":[251],"results":[252],"supported":[253],"BMP":[255],"calculations":[256],"samples":[259],"study.":[262],"Biogas":[263],"yields":[264],"improved":[267],"if":[268],"employed":[273],"routine":[275],"before":[277],"co-digesting":[278],"different":[279],"substrates.":[280]},"counts_by_year":[{"year":2026,"cited_by_count":7},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":13},{"year":2023,"cited_by_count":11},{"year":2022,"cited_by_count":9}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2025-10-10T00:00:00"}