{"id":"https://openalex.org/W3202852589","doi":"https://doi.org/10.1007/s00299-021-02796-0","title":"Optimization of T-DNA configuration with UBIQUITIN10 promoters and tRNA\u2013sgRNA complexes promotes highly efficient genome editing in allotetraploid tobacco","display_name":"Optimization of T-DNA configuration with UBIQUITIN10 promoters and tRNA\u2013sgRNA complexes promotes highly efficient genome editing in allotetraploid tobacco","publication_year":2021,"publication_date":"2021-10-08","ids":{"openalex":"https://openalex.org/W3202852589","doi":"https://doi.org/10.1007/s00299-021-02796-0","mag":"3202852589","pmid":"https://pubmed.ncbi.nlm.nih.gov/34623476"},"language":"en","primary_location":{"id":"doi:10.1007/s00299-021-02796-0","doi":"https://doi.org/10.1007/s00299-021-02796-0","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s00299-021-02796-0","pdf_url":null,"source":{"id":"https://openalex.org/S196783408","display_name":"Plant Cell Reports","issn_l":"0721-7714","issn":["0721-7714","1432-203X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Cell Reports","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5091297252","display_name":"Manoj Kumar","orcid":"https://orcid.org/0000-0001-5970-2902","observed_orcids":["https://orcid.org/0000-0001-5970-2902"]},"institutions":[{"id":"https://openalex.org/I376102","display_name":"Agricultural Research Organization","ror":"https://ror.org/05hbrxp80","country_code":"IL","type":"government","lineage":["https://openalex.org/I376102"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Manoj Kumar","raw_affiliation_strings":["Department of Ornamental Plants and Agricultural Biotechnology, The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeZion, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ornamental Plants and Agricultural Biotechnology, The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeZion, Israel","institution_ids":["https://openalex.org/I376102"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069390391","display_name":"Dana Ayzenshtat","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I376102","display_name":"Agricultural Research Organization","ror":"https://ror.org/05hbrxp80","country_code":"IL","type":"government","lineage":["https://openalex.org/I376102"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Dana Ayzenshtat","raw_affiliation_strings":["Department of Ornamental Plants and Agricultural Biotechnology, The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeZion, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ornamental Plants and Agricultural Biotechnology, The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeZion, Israel","institution_ids":["https://openalex.org/I376102"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007053521","display_name":"Adar Marko","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I376102","display_name":"Agricultural Research Organization","ror":"https://ror.org/05hbrxp80","country_code":"IL","type":"government","lineage":["https://openalex.org/I376102"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Adar Marko","raw_affiliation_strings":["Department of Ornamental Plants and Agricultural Biotechnology, The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeZion, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ornamental Plants and Agricultural Biotechnology, The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeZion, Israel","institution_ids":["https://openalex.org/I376102"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5087666494","display_name":"Samuel E. Bocobza","orcid":"https://orcid.org/0000-0002-8138-4426","observed_orcids":["https://orcid.org/0000-0002-8138-4426"]},"institutions":[{"id":"https://openalex.org/I376102","display_name":"Agricultural Research Organization","ror":"https://ror.org/05hbrxp80","country_code":"IL","type":"government","lineage":["https://openalex.org/I376102"]}],"countries":["IL"],"is_corresponding":true,"raw_author_name":"Samuel Bocobza","raw_affiliation_strings":["Department of Ornamental Plants and Agricultural Biotechnology, The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeZion, Israel. bocobza@volcani.agri.gov.il","Department of Ornamental Plants and Agricultural Biotechnology, The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeZion, Israel"],"raw_orcid":"https://orcid.org/0000-0002-8138-4426","affiliations":[{"raw_affiliation_string":"Department of Ornamental Plants and Agricultural Biotechnology, The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeZion, Israel. bocobza@volcani.agri.gov.il","institution_ids":["https://openalex.org/I376102"]},{"raw_affiliation_string":"Department of Ornamental Plants and Agricultural Biotechnology, The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeZion, Israel","institution_ids":["https://openalex.org/I376102"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5087666494"],"corresponding_institution_ids":["https://openalex.org/I376102"],"apc_list":{"value":3860,"currency":"USD","value_usd":3860},"apc_paid":null,"fwci":0.9088,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.6957689,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":95,"max":99},"biblio":{"volume":"41","issue":"1","first_page":"175","last_page":"194"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9897000193595886,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9897000193595886,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.004900000058114529,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9-genome-editing","display_name":"CRISPR/Cas9 genome editing","score":0.8379999995231628},{"id":"https://openalex.org/keywords/nicotiana-tabacum","display_name":"tobacco","score":0.9980000257492065},{"id":"https://openalex.org/keywords/transgene-expression","display_name":"transgene expression","score":0.7089999914169312},{"id":"https://openalex.org/keywords/transgene-silencing","display_name":"transgene silencing","score":0.6460000276565552},{"id":"https://openalex.org/keywords/solanaceae","display_name":"Solanaceae","score":0.6679999828338623}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9001463651657104},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.6735193133354187},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.6531904339790344},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.6396962404251099},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5861191749572754},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.5627400279045105},{"id":"https://openalex.org/C2781205006","wikidata":"https://www.wikidata.org/wiki/Q181095","display_name":"Nicotiana tabacum","level":3,"score":0.5507538914680481},{"id":"https://openalex.org/C189819185","wikidata":"https://www.wikidata.org/wiki/Q384283","display_name":"Subgenomic mRNA","level":4,"score":0.5241461396217346},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.514579713344574},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5097913146018982},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.49163299798965454},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.46290668845176697},{"id":"https://openalex.org/C77957584","wikidata":"https://www.wikidata.org/wiki/Q1133550","display_name":"Gene knockout","level":3,"score":0.45549947023391724},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.35440361499786377},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.31092071533203125}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014026","descriptor_name":"Nicotiana","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014452","descriptor_name":"Ubiquitins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014452","descriptor_name":"Ubiquitins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018744","descriptor_name":"DNA, Plant","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D018744","descriptor_name":"DNA, Plant","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018745","descriptor_name":"Genome, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018749","descriptor_name":"RNA, Plant","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D018749","descriptor_name":"RNA, Plant","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D057891","descriptor_name":"Tetraploidy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/s00299-021-02796-0","doi":"https://doi.org/10.1007/s00299-021-02796-0","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s00299-021-02796-0","pdf_url":null,"source":{"id":"https://openalex.org/S196783408","display_name":"Plant Cell Reports","issn_l":"0721-7714","issn":["0721-7714","1432-203X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Cell Reports","raw_type":"journal-article"},{"id":"pmid:34623476","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34623476","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant cell reports","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.5365999937057495,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G256235209","display_name":null,"funder_award_id":"20\u201001\u20100209","funder_id":"https://openalex.org/F4320322739","funder_display_name":"Ministry of Agriculture and Rural Development"}],"funders":[{"id":"https://openalex.org/F4320322739","display_name":"Ministry of Agriculture and Rural Development","ror":"https://ror.org/05e6hcm17"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":111,"referenced_works":["https://openalex.org/W84311565","https://openalex.org/W167748462","https://openalex.org/W1510606824","https://openalex.org/W1572946039","https://openalex.org/W1591420606","https://openalex.org/W1783181079","https://openalex.org/W1860470769","https://openalex.org/W1882933654","https://openalex.org/W1885181197","https://openalex.org/W1921003950","https://openalex.org/W1943210967","https://openalex.org/W1951228636","https://openalex.org/W1965853301","https://openalex.org/W1970893992","https://openalex.org/W1971229919","https://openalex.org/W1974954117","https://openalex.org/W1980201684","https://openalex.org/W1981540614","https://openalex.org/W1986135627","https://openalex.org/W1986405290","https://openalex.org/W1986717620","https://openalex.org/W1990379499","https://openalex.org/W1991710875","https://openalex.org/W1992783297","https://openalex.org/W2000768580","https://openalex.org/W2001845502","https://openalex.org/W2002171861","https://openalex.org/W2002712030","https://openalex.org/W2005839167","https://openalex.org/W2010763778","https://openalex.org/W2011739387","https://openalex.org/W2014174829","https://openalex.org/W2021767455","https://openalex.org/W2024556021","https://openalex.org/W2026273254","https://openalex.org/W2028592324","https://openalex.org/W2030617019","https://openalex.org/W2030984741","https://openalex.org/W2035270026","https://openalex.org/W2035680796","https://openalex.org/W2036028864","https://openalex.org/W2040665850","https://openalex.org/W2042064790","https://openalex.org/W2045439285","https://openalex.org/W2046576215","https://openalex.org/W2046639072","https://openalex.org/W2057847071","https://openalex.org/W2077152657","https://openalex.org/W2080757130","https://openalex.org/W2081692784","https://openalex.org/W2086491289","https://openalex.org/W2090230257","https://openalex.org/W2093247158","https://openalex.org/W2093481141","https://openalex.org/W2095180061","https://openalex.org/W2107913200","https://openalex.org/W2110556684","https://openalex.org/W2113385887","https://openalex.org/W2114280998","https://openalex.org/W2115189270","https://openalex.org/W2115603949","https://openalex.org/W2127313385","https://openalex.org/W2132051679","https://openalex.org/W2133836136","https://openalex.org/W2135008913","https://openalex.org/W2136656609","https://openalex.org/W2153186861","https://openalex.org/W2155989425","https://openalex.org/W2156102046","https://openalex.org/W2158215557","https://openalex.org/W2159586784","https://openalex.org/W2163820831","https://openalex.org/W2165016943","https://openalex.org/W2191974075","https://openalex.org/W2196121581","https://openalex.org/W2207565062","https://openalex.org/W2240027552","https://openalex.org/W2264491011","https://openalex.org/W2337751047","https://openalex.org/W2349412605","https://openalex.org/W2398269215","https://openalex.org/W2471889160","https://openalex.org/W2484320633","https://openalex.org/W2515114895","https://openalex.org/W2515143162","https://openalex.org/W2541675569","https://openalex.org/W2568479992","https://openalex.org/W2569391057","https://openalex.org/W2579163968","https://openalex.org/W2622892549","https://openalex.org/W2731682345","https://openalex.org/W2782595117","https://openalex.org/W2782905727","https://openalex.org/W2783310185","https://openalex.org/W2803479625","https://openalex.org/W2890617934","https://openalex.org/W2895213500","https://openalex.org/W2912430338","https://openalex.org/W2912796099","https://openalex.org/W2915630863","https://openalex.org/W2952594972","https://openalex.org/W2973187420","https://openalex.org/W2989848563","https://openalex.org/W2991141137","https://openalex.org/W3000527518","https://openalex.org/W3042562775","https://openalex.org/W3109531730","https://openalex.org/W3118169118","https://openalex.org/W3133801250","https://openalex.org/W3179208630","https://openalex.org/W4211210980"],"related_works":["https://openalex.org/W4280489565","https://openalex.org/W4308295089","https://openalex.org/W4394566742","https://openalex.org/W4283027238","https://openalex.org/W7161757773","https://openalex.org/W2003240933","https://openalex.org/W4206045745","https://openalex.org/W3041162101","https://openalex.org/W4386084972","https://openalex.org/W2989848563"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":8},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":6}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2021-10-11T00:00:00"}
