{"id":"https://openalex.org/W3202740459","doi":"https://doi.org/10.1111/ijlh.13711","title":"Comparison of flow cytometry and next\u2010generation sequencing in minimal residual disease monitoring of acute myeloid leukemia: One institute\u2019s practical clinical experience","display_name":"Comparison of flow cytometry and next\u2010generation sequencing in minimal residual disease monitoring of acute myeloid leukemia: One institute\u2019s practical clinical experience","publication_year":2021,"publication_date":"2021-09-29","ids":{"openalex":"https://openalex.org/W3202740459","doi":"https://doi.org/10.1111/ijlh.13711","mag":"3202740459","pmid":"https://pubmed.ncbi.nlm.nih.gov/34585519"},"language":"en","primary_location":{"id":"doi:10.1111/ijlh.13711","doi":"https://doi.org/10.1111/ijlh.13711","is_oa":false,"landing_page_url":"https://doi.org/10.1111/ijlh.13711","pdf_url":null,"source":{"id":"https://openalex.org/S66208518","display_name":"International Journal of Laboratory Hematology","issn_l":"1751-5521","issn":["1751-5521","1751-553X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Laboratory Hematology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5050327708","display_name":"Paul McGowan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paul F. McGowan","raw_affiliation_strings":["Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","institution_ids":["https://openalex.org/I4210128618"]},{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058265159","display_name":"Stephen D. Hyter","orcid":"https://orcid.org/0000-0001-8176-9644","observed_orcids":["https://orcid.org/0000-0001-8176-9644"]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephen D. Hyter","raw_affiliation_strings":["Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","institution_ids":["https://openalex.org/I4210128618"]},{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016471127","display_name":"Wei Cui","orcid":"https://orcid.org/0000-0002-8177-0538","observed_orcids":["https://orcid.org/0000-0002-8177-0538"]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wei Cui","raw_affiliation_strings":["Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA"],"raw_orcid":"https://orcid.org/0000-0002-8177-0538","affiliations":[{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","institution_ids":["https://openalex.org/I4210128618"]},{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087567207","display_name":"Regina M. Plummer","orcid":"https://orcid.org/0000-0001-6183-4779","observed_orcids":["https://orcid.org/0000-0001-6183-4779"]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Regina M. Plummer","raw_affiliation_strings":["Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","institution_ids":["https://openalex.org/I4210128618"]},{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033625395","display_name":"Andrew K. Godwin","orcid":"https://orcid.org/0000-0002-3987-9580","observed_orcids":["https://orcid.org/0000-0002-3987-9580"]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew K. Godwin","raw_affiliation_strings":["Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","institution_ids":["https://openalex.org/I4210128618"]},{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101882204","display_name":"Da Zhang","orcid":"https://orcid.org/0000-0001-5454-7763","observed_orcids":["https://orcid.org/0000-0001-5454-7763"]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Da Zhang","raw_affiliation_strings":["Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA"],"raw_orcid":"https://orcid.org/0000-0001-5454-7763","affiliations":[{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA","institution_ids":["https://openalex.org/I4210128618"]},{"raw_affiliation_string":"Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA","institution_ids":["https://openalex.org/I4210128618"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5101882204"],"corresponding_institution_ids":["https://openalex.org/I4210128618"],"apc_list":{"value":3400,"currency":"USD","value_usd":3400},"apc_paid":null,"fwci":1.1406,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.78173011,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":98},"biblio":{"volume":"44","issue":"1","first_page":"118","last_page":"126"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10950","display_name":"Acute Lymphoblastic Leukemia research","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11559","display_name":"Retinoids in leukemia and cellular processes","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/acute-myeloid-leukemia","display_name":"acute myeloid leukemia","score":0.9810000061988831},{"id":"https://openalex.org/keywords/measurable-residual-disease","display_name":"minimal residual disease","score":0.9909999966621399},{"id":"https://openalex.org/keywords/flow-cytometry","display_name":"flow cytometry","score":0.902999997138977},{"id":"https://openalex.org/keywords/next-generation-sequencing","display_name":"next-generation sequencing","score":0.9940000176429749},{"id":"https://openalex.org/keywords/molecular-monitoring","display_name":"molecular monitoring","score":0.5049999952316284},{"id":"https://openalex.org/keywords/clonal-hematopoiesis","display_name":"clonal hematopoiesis","score":0.8560000061988831},{"id":"https://openalex.org/keywords/variants-of-uncertain-significance","display_name":"variants of uncertain significance","score":0.7269999980926514}],"concepts":[{"id":"https://openalex.org/C2779823535","wikidata":"https://www.wikidata.org/wiki/Q6865356","display_name":"Minimal residual disease","level":3,"score":0.8352196216583252},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.7587522268295288},{"id":"https://openalex.org/C2778729363","wikidata":"https://www.wikidata.org/wiki/Q11688946","display_name":"Myeloid leukemia","level":2,"score":0.6228258609771729},{"id":"https://openalex.org/C2779282312","wikidata":"https://www.wikidata.org/wiki/Q1956561","display_name":"Myeloid","level":2,"score":0.5778957605361938},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.5766534805297852},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.4748024046421051},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.4399679899215698},{"id":"https://openalex.org/C2778461978","wikidata":"https://www.wikidata.org/wiki/Q29496","display_name":"Leukemia","level":2,"score":0.42101335525512695},{"id":"https://openalex.org/C3018877446","wikidata":"https://www.wikidata.org/wiki/Q1421432","display_name":"Disease monitoring","level":3,"score":0.41394394636154175},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3735818862915039},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.352486252784729},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.3242700695991516},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.27454811334609985},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.19939187169075012},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.15423747897148132},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.15184497833251953},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1364263892173767}],"mesh":[{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012189","descriptor_name":"Retrospective Studies","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014408","descriptor_name":"Biomarkers, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015470","descriptor_name":"Leukemia, Myeloid, Acute","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D015470","descriptor_name":"Leukemia, Myeloid, Acute","qualifier_ui":"Q000209","qualifier_name":"etiology","is_major_topic":false},{"descriptor_ui":"D015470","descriptor_name":"Leukemia, Myeloid, Acute","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016130","descriptor_name":"Immunophenotyping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018365","descriptor_name":"Neoplasm, Residual","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D019468","descriptor_name":"Disease Management","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1111/ijlh.13711","doi":"https://doi.org/10.1111/ijlh.13711","is_oa":false,"landing_page_url":"https://doi.org/10.1111/ijlh.13711","pdf_url":null,"source":{"id":"https://openalex.org/S66208518","display_name":"International Journal of Laboratory Hematology","issn_l":"1751-5521","issn":["1751-5521","1751-553X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Laboratory Hematology","raw_type":"journal-article"},{"id":"pmid:34585519","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34585519","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of laboratory hematology","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9537000060081482,"id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[{"display_name":"Good health and well-being","score":0.4300000071525574,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[{"display_name":"Observational Study","id":"https://openalex.org/study-designs/observational-study"}],"awards":[],"funders":[{"id":"https://openalex.org/F4320332445","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W1513924279","https://openalex.org/W1747932062","https://openalex.org/W1920829940","https://openalex.org/W1997412169","https://openalex.org/W2051978340","https://openalex.org/W2072404566","https://openalex.org/W2110096889","https://openalex.org/W2153870379","https://openalex.org/W2276580523","https://openalex.org/W2419140434","https://openalex.org/W2528238909","https://openalex.org/W2559496536","https://openalex.org/W2760499085","https://openalex.org/W2783450169","https://openalex.org/W2794480810","https://openalex.org/W2890272121","https://openalex.org/W2922336474","https://openalex.org/W2928266645","https://openalex.org/W2950498014","https://openalex.org/W2980900748","https://openalex.org/W3013889537","https://openalex.org/W3131323362"],"related_works":["https://openalex.org/W7161412899","https://openalex.org/W4405054863","https://openalex.org/W4382011447","https://openalex.org/W4379333361","https://openalex.org/W3214189228","https://openalex.org/W2604039584","https://openalex.org/W2885961002","https://openalex.org/W2225929878","https://openalex.org/W1970434092","https://openalex.org/W2765292430"],"abstract_inverted_index":{"INTRODUCTION:":[0],"Monitoring":[1],"patients":[2,60],"with":[3,70,128,150],"acute":[4],"myeloid":[5],"leukemia":[6],"can":[7],"be":[8,139],"implemented":[9],"through":[10],"various":[11],"techniques":[12],"such":[13],"as":[14],"multiparameter":[15],"flow":[16,39,123,151],"cytometry,":[17],"real-time":[18],"quantitative":[19],"polymerase":[20],"chain":[21],"reaction,":[22],"and":[23,38,67,76,144],"next-generation":[24,36],"sequencing.":[25],"However,":[26],"there":[27],"is":[28],"scarce":[29],"studies":[30,79],"when":[31,52],"comparing":[32],"the":[33],"data":[34,69],"of":[35,73,115,159],"sequencing":[37,137],"cytometry":[40,124,152],"for":[41],"monitoring":[42,85],"disease":[43],"progression,":[44],"particularly":[45],"how":[46],"they":[47],"might":[48],"supplement":[49],"one":[50],"another":[51],"used":[53,140],"in":[54,84,131,141,148,163],"tandem.":[55],"METHODS:":[56],"We":[57],"investigated":[58],"107":[59],"via":[61],"retrospective":[62],"analysis":[63],"using":[64],"follow-up":[65],"MFC":[66,75],"NGS":[68,78,94],"a":[71,155],"total":[72],"717":[74],"247":[77],"to":[80,96,153],"compare":[81],"these":[82,160],"methods":[83],"minimal/measurable":[86,133],"residual":[87,134],"disease.":[88,135],"RESULTS:":[89],"instances,":[90],"13":[91],"had":[92],"similar":[93],"profiles":[95],"their":[97],"original":[98],"day":[99],"0":[100],"diagnosis.":[101],"The":[102],"remaining":[103],"cases":[104],"showed":[105],"preleukemic":[106],"clonal":[107],"hematopoiesis":[108],"mutations,":[109],"\"likely":[110],"pathogenic":[111],"mutations,\"":[112],"or":[113],"\"variants":[114],"uncertain":[116],"significance.\"":[117],"CONCLUSION:":[118],"Our":[119],"findings":[120],"show":[121],"that":[122],"has":[125],"its":[126],"advantages":[127],"comparable":[129],"sensitivity":[130],"detecting":[132],"Next-generation":[136],"could":[138],"an":[142],"increased":[143],"more":[145,156],"regular":[146],"capacity":[147],"conjunction":[149],"achieve":[154],"comprehensive":[157],"surveillance":[158],"patients,":[161],"resulting":[162],"improved":[164],"outcomes.":[165]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2021-10-11T00:00:00"}