{"id":"https://openalex.org/W3202259972","doi":"https://doi.org/10.3389/fimmu.2021.730766","title":"A Barcoded Flow Cytometric Assay to Explore the Antibody Responses Against SARS-CoV-2 Spike and Its Variants","display_name":"A Barcoded Flow Cytometric Assay to Explore the Antibody Responses Against SARS-CoV-2 Spike and Its Variants","publication_year":2021,"publication_date":"2021-09-23","ids":{"openalex":"https://openalex.org/W3202259972","doi":"https://doi.org/10.3389/fimmu.2021.730766","mag":"3202259972","pmid":"https://pubmed.ncbi.nlm.nih.gov/34630410","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8496935"},"language":"en","primary_location":{"id":"doi:10.3389/fimmu.2021.730766","doi":"https://doi.org/10.3389/fimmu.2021.730766","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fimmu.2021.730766","pdf_url":"https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.730766/pdf","source":{"id":"https://openalex.org/S2595292759","display_name":"Frontiers in Immunology","issn_l":"1664-3224","issn":["1664-3224"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Immunology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.730766/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5006908636","display_name":"Niklas Vesper","orcid":"https://orcid.org/0009-0006-8165-0612","observed_orcids":["https://orcid.org/0009-0006-8165-0612"]},"institutions":[{"id":"https://openalex.org/I161046081","display_name":"University of Freiburg","ror":"https://ror.org/0245cg223","country_code":"DE","type":"education","lineage":["https://openalex.org/I161046081"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Niklas Vesper","raw_affiliation_strings":["Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany","Research Centres Bioss, Centre for Biological signal studies, CIBSS, Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081"]},{"raw_affiliation_string":"Research Centres Bioss, Centre for Biological signal studies, CIBSS, Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002531545","display_name":"Yaneth M. Ortiz","orcid":"https://orcid.org/0000-0002-1229-761X","observed_orcids":["https://orcid.org/0000-0002-1229-761X"]},"institutions":[{"id":"https://openalex.org/I161046081","display_name":"University of Freiburg","ror":"https://ror.org/0245cg223","country_code":"DE","type":"education","lineage":["https://openalex.org/I161046081"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Yaneth Ortiz","raw_affiliation_strings":["Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany","Research Centres Bioss, Centre for Biological signal studies, CIBSS, Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081"]},{"raw_affiliation_string":"Research Centres Bioss, Centre for Biological signal studies, CIBSS, Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052161120","display_name":"Frauke Bartels-Burgahn","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161046081","display_name":"University of Freiburg","ror":"https://ror.org/0245cg223","country_code":"DE","type":"education","lineage":["https://openalex.org/I161046081"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Frauke Bartels-Burgahn","raw_affiliation_strings":["Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany","Research Centres Bioss, Centre for Biological signal studies, CIBSS, Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081"]},{"raw_affiliation_string":"Research Centres Bioss, Centre for Biological signal studies, CIBSS, Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062482652","display_name":"Jianying Yang","orcid":"https://orcid.org/0000-0001-8197-5413","observed_orcids":["https://orcid.org/0000-0001-8197-5413"]},"institutions":[{"id":"https://openalex.org/I161046081","display_name":"University of Freiburg","ror":"https://ror.org/0245cg223","country_code":"DE","type":"education","lineage":["https://openalex.org/I161046081"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Jianying Yang","raw_affiliation_strings":["Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany","Research Centres Bioss, Centre for Biological signal studies, CIBSS, Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081"]},{"raw_affiliation_string":"Research Centres Bioss, Centre for Biological signal studies, CIBSS, Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002158875","display_name":"Kathrin de la Rosa","orcid":"https://orcid.org/0000-0003-4809-3157","observed_orcids":["https://orcid.org/0000-0003-4809-3157"]},"institutions":[{"id":"https://openalex.org/I205582932","display_name":"Max Delbr\u00fcck Center","ror":"https://ror.org/04p5ggc03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I205582932"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Kathrin de la Rosa","raw_affiliation_strings":["Department of Cancer and Immunology, Max-Delbr\u00fcck-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cancer and Immunology, Max-Delbr\u00fcck-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany","institution_ids":["https://openalex.org/I205582932"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051481020","display_name":"Matthias Tenbusch","orcid":"https://orcid.org/0000-0003-3951-9056","observed_orcids":["https://orcid.org/0000-0003-3951-9056"]},"institutions":[{"id":"https://openalex.org/I181369854","display_name":"Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg","ror":"https://ror.org/00f7hpc57","country_code":"DE","type":"education","lineage":["https://openalex.org/I181369854"]},{"id":"https://openalex.org/I4210088053","display_name":"Universit\u00e4tsklinikum Erlangen","ror":"https://ror.org/0030f2a11","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I4210088053"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Matthias Tenbusch","raw_affiliation_strings":["Institute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany","institution_ids":["https://openalex.org/I181369854","https://openalex.org/I4210088053"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044270582","display_name":"Sebastian R. Schulz","orcid":"https://orcid.org/0000-0001-6554-3346","observed_orcids":["https://orcid.org/0000-0001-6554-3346"]},"institutions":[{"id":"https://openalex.org/I181369854","display_name":"Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg","ror":"https://ror.org/00f7hpc57","country_code":"DE","type":"education","lineage":["https://openalex.org/I181369854"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Sebastian Schulz","raw_affiliation_strings":["Division of Molecular Immunology, Internal Medicine III, Nikolaus-Fiebiger-Center of Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Molecular Immunology, Internal Medicine III, Nikolaus-Fiebiger-Center of Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany","institution_ids":["https://openalex.org/I181369854"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013492221","display_name":"Stephanie Finzel","orcid":"https://orcid.org/0000-0002-7625-9612","observed_orcids":["https://orcid.org/0000-0002-7625-9612"]},"institutions":[{"id":"https://openalex.org/I161046081","display_name":"University of Freiburg","ror":"https://ror.org/0245cg223","country_code":"DE","type":"education","lineage":["https://openalex.org/I161046081"]},{"id":"https://openalex.org/I2801240073","display_name":"University Medical Center Freiburg","ror":"https://ror.org/03vzbgh69","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I2801240073"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Stephanie Finzel","raw_affiliation_strings":["Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081","https://openalex.org/I2801240073"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066746944","display_name":"Hans\u2010Martin J\u00e4ck","orcid":"https://orcid.org/0000-0002-6332-8463","observed_orcids":["https://orcid.org/0000-0002-6332-8463"]},"institutions":[{"id":"https://openalex.org/I181369854","display_name":"Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg","ror":"https://ror.org/00f7hpc57","country_code":"DE","type":"education","lineage":["https://openalex.org/I181369854"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Hans-Martin J\u00e4ck","raw_affiliation_strings":["Division of Molecular Immunology, Internal Medicine III, Nikolaus-Fiebiger-Center of Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Molecular Immunology, Internal Medicine III, Nikolaus-Fiebiger-Center of Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany","institution_ids":["https://openalex.org/I181369854"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018033673","display_name":"Hermann Eibel","orcid":"https://orcid.org/0000-0003-2901-2809","observed_orcids":["https://orcid.org/0000-0003-2901-2809"]},"institutions":[{"id":"https://openalex.org/I161046081","display_name":"University of Freiburg","ror":"https://ror.org/0245cg223","country_code":"DE","type":"education","lineage":["https://openalex.org/I161046081"]},{"id":"https://openalex.org/I2801240073","display_name":"University Medical Center Freiburg","ror":"https://ror.org/03vzbgh69","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I2801240073"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Hermann Eibel","raw_affiliation_strings":["Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, Freiburg, Germany","Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081","https://openalex.org/I2801240073"]},{"raw_affiliation_string":"Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081","https://openalex.org/I2801240073"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004962774","display_name":"Reinhard Edmund Voll","orcid":"https://orcid.org/0000-0002-5542-9133","observed_orcids":["https://orcid.org/0000-0002-5542-9133"]},"institutions":[{"id":"https://openalex.org/I161046081","display_name":"University of Freiburg","ror":"https://ror.org/0245cg223","country_code":"DE","type":"education","lineage":["https://openalex.org/I161046081"]},{"id":"https://openalex.org/I2801240073","display_name":"University Medical Center Freiburg","ror":"https://ror.org/03vzbgh69","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I2801240073"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Reinhard E. Voll","raw_affiliation_strings":["Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, Freiburg, Germany","Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081","https://openalex.org/I2801240073"]},{"raw_affiliation_string":"Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081","https://openalex.org/I2801240073"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013470603","display_name":"Michael G. Reth","orcid":"https://orcid.org/0000-0002-1025-7198","observed_orcids":["https://orcid.org/0000-0002-1025-7198"]},"institutions":[{"id":"https://openalex.org/I161046081","display_name":"University of Freiburg","ror":"https://ror.org/0245cg223","country_code":"DE","type":"education","lineage":["https://openalex.org/I161046081"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Michael Reth","raw_affiliation_strings":["Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany","Research Centres Bioss, Centre for Biological signal studies, CIBSS, Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081"]},{"raw_affiliation_string":"Research Centres Bioss, Centre for Biological signal studies, CIBSS, Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany","institution_ids":["https://openalex.org/I161046081"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5013470603"],"corresponding_institution_ids":["https://openalex.org/I161046081"],"apc_list":{"value":2950,"currency":"USD","value_usd":2950},"apc_paid":{"value":2950,"currency":"USD","value_usd":2950},"fwci":0.3918,"has_fulltext":true,"cited_by_count":5,"citation_normalized_percentile":{"value":0.64635971,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"12","issue":null,"first_page":"730766","last_page":"730766"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10118","display_name":"SARS-CoV-2 and COVID-19 Research","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10118","display_name":"SARS-CoV-2 and COVID-19 Research","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11754","display_name":"SARS-CoV-2 detection and testing","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12576","display_name":"vaccines and immunoinformatics approaches","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sars-cov-2","display_name":"SARS-CoV-2","score":0.9570000171661377},{"id":"https://openalex.org/keywords/covid-19","display_name":"COVID-19 pandemic","score":0.8370000123977661},{"id":"https://openalex.org/keywords/sars-cov-2-variants","display_name":"SARS-CoV-2 variants","score":0.8299999833106995},{"id":"https://openalex.org/keywords/spike-protein","display_name":"spike protein","score":0.9430000185966492},{"id":"https://openalex.org/keywords/receptor-binding-domain","display_name":"receptor binding domain","score":0.949999988079071},{"id":"https://openalex.org/keywords/humoral-immune-response","display_name":"humoral immune response","score":0.7680000066757202},{"id":"https://openalex.org/keywords/antibody-response","display_name":"antibody response","score":0.47999998927116394},{"id":"https://openalex.org/keywords/ace2-binding-affinity","display_name":"ACE2 binding affinity","score":0.7829999923706055},{"id":"https://openalex.org/keywords/n501y-mutation","display_name":"N501Y mutation","score":0.9049999713897705},{"id":"https://openalex.org/keywords/e484k-mutation","display_name":"E484K mutation","score":0.9990000128746033},{"id":"https://openalex.org/keywords/alpha-variant","display_name":"Alpha variant","score":0.7459999918937683},{"id":"https://openalex.org/keywords/beta-variant","display_name":"Beta variant","score":1.0},{"id":"https://openalex.org/keywords/immune-evasion","display_name":"immune evasion","score":0.8100000023841858},{"id":"https://openalex.org/keywords/covid-19-vaccination","display_name":"COVID-19 vaccination","score":0.7900000214576721},{"id":"https://openalex.org/keywords/igg","display_name":"IgG","score":0.75},{"id":"https://openalex.org/keywords/iga","display_name":"IgA","score":1.0}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6175781488418579},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.6165882349014282},{"id":"https://openalex.org/C167672396","wikidata":"https://www.wikidata.org/wiki/Q417800","display_name":"CD8","level":3,"score":0.6031370162963867},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5479108095169067},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5391071438789368},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5023908615112305},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.49739959836006165},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.4851408898830414},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.423193097114563},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3951943814754486},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.380222350358963},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2253706455230713},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.20121461153030396},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.16382616758346558},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.1542392373085022},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000085962","descriptor_name":"Angiotensin-Converting Enzyme 2","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000086382","descriptor_name":"COVID-19","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D000914","descriptor_name":"Antibodies, Viral","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000917","descriptor_name":"Antibody Formation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007070","descriptor_name":"Immunoglobulin A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007074","descriptor_name":"Immunoglobulin G","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007117","descriptor_name":"Immunization, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014611","descriptor_name":"Vaccination","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064370","descriptor_name":"Spike Glycoprotein, Coronavirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D064370","descriptor_name":"Spike Glycoprotein, Coronavirus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":7,"locations":[{"id":"doi:10.3389/fimmu.2021.730766","doi":"https://doi.org/10.3389/fimmu.2021.730766","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fimmu.2021.730766","pdf_url":"https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.730766/pdf","source":{"id":"https://openalex.org/S2595292759","display_name":"Frontiers in Immunology","issn_l":"1664-3224","issn":["1664-3224"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Immunology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:7389138","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC8496935","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:8496935","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8496935","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Immunol","raw_type":"Text"},{"id":"pmid:34630410","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34630410","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in immunology","raw_type":"Journal Article"},{"id":"pmh:oai:edoc.mdc-berlin.de:20965","doi":null,"is_oa":false,"landing_page_url":"http://edoc.mdc-berlin.de/20965/5/20965suppl.zip","pdf_url":null,"source":{"id":"https://openalex.org/S4306400224","display_name":"MDC Repository (Max-Delbrueck-Center for Molecular Medicine)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I149899117","host_organization_name":"Max Planck Society","host_organization_lineage":["https://openalex.org/I149899117"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Article"},{"id":"pmh:oai:doaj.org/article:8028980ae84e44d8981c50d0be50140f","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/8028980ae84e44d8981c50d0be50140f","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Immunology, Vol 12 (2021)","raw_type":"article"},{"id":"pmh:oai:ub.uni-erlangen.de-opus:17346","doi":null,"is_oa":true,"landing_page_url":"https://opus4.kobv.de/opus4-fau/frontdoor/index/index/docId/17346","pdf_url":null,"source":{"id":"https://openalex.org/S4306401636","display_name":"OPUS Repository (Kooperativer Bibliotheksverbund Berlin-Brandenburg)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fimmu.2021.730766","doi":"https://doi.org/10.3389/fimmu.2021.730766","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fimmu.2021.730766","pdf_url":"https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.730766/pdf","source":{"id":"https://openalex.org/S2595292759","display_name":"Frontiers in Immunology","issn_l":"1664-3224","issn":["1664-3224"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Immunology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8560000061988831}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2512731383","display_name":null,"funder_award_id":"GRK 1660","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"},{"id":"https://openalex.org/G3151753491","display_name":null,"funder_award_id":"01KX2021","funder_id":"https://openalex.org/F4320331433","funder_display_name":"Bayerisches Staatsministerium f\u00fcr Wissenschaft und Kunst"},{"id":"https://openalex.org/G3401020598","display_name":null,"funder_award_id":"TRR130","funder_id":"https://openalex.org/F4320321450","funder_display_name":"Bayerische Forschungsstiftung"},{"id":"https://openalex.org/G551823739","display_name":null,"funder_award_id":"TRR130","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"},{"id":"https://openalex.org/G553083828","display_name":null,"funder_award_id":"01KX2021","funder_id":"https://openalex.org/F4320321450","funder_display_name":"Bayerische Forschungsstiftung"},{"id":"https://openalex.org/G56512868","display_name":"Humane Antik\u00f6rper: Von nat\u00fcrlichem und artifiziellem Engineering zur Analyse von Repertoires","funder_award_id":"394523286","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"},{"id":"https://openalex.org/G5791815451","display_name":null,"funder_award_id":"01KX2021","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"}],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"},{"id":"https://openalex.org/F4320321450","display_name":"Bayerische Forschungsstiftung","ror":"https://ror.org/00emnts94"},{"id":"https://openalex.org/F4320331433","display_name":"Bayerisches Staatsministerium f\u00fcr Wissenschaft und Kunst","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3202259972.pdf","grobid_xml":"https://content.openalex.org/works/W3202259972.grobid-xml"},"referenced_works_count":31,"referenced_works":["https://openalex.org/W1591208477","https://openalex.org/W2799959526","https://openalex.org/W2892199161","https://openalex.org/W3006594788","https://openalex.org/W3007643904","https://openalex.org/W3009335299","https://openalex.org/W3009906937","https://openalex.org/W3009912996","https://openalex.org/W3013093478","https://openalex.org/W3033966524","https://openalex.org/W3039066506","https://openalex.org/W3086125063","https://openalex.org/W3092136311","https://openalex.org/W3092192687","https://openalex.org/W3092403777","https://openalex.org/W3093260768","https://openalex.org/W3108500958","https://openalex.org/W3112389805","https://openalex.org/W3123108673","https://openalex.org/W3123551159","https://openalex.org/W3124492191","https://openalex.org/W3124827150","https://openalex.org/W3127518626","https://openalex.org/W3130156934","https://openalex.org/W3133525304","https://openalex.org/W3136588845","https://openalex.org/W3161511556","https://openalex.org/W3165479483","https://openalex.org/W3170598451","https://openalex.org/W3171375949","https://openalex.org/W3192159682"],"related_works":["https://openalex.org/W3215818195","https://openalex.org/W4386954639","https://openalex.org/W4285739889","https://openalex.org/W4384037040","https://openalex.org/W4210858720","https://openalex.org/W6998918935","https://openalex.org/W4403906390","https://openalex.org/W4294191236","https://openalex.org/W4225614889","https://openalex.org/W6982386531"],"abstract_inverted_index":{"The":[0,196,278],"SARS-CoV-2":[1,321],"pandemic":[2],"has":[3,27],"spread":[4,36],"to":[5,33,63,84,165,228,237,247,254,312,323],"all":[6],"parts":[7],"of":[8,37,109,125,177,198,203,230,276,320],"the":[9,35,46,49,61,65,89,105,114,140,150,155,166,178,182,190,199,214,216,223,231,239,248,255,265,268,287,293,298,314],"world":[10],"and":[11,16,53,60,86,142,186,192,251,290,322],"can":[12],"cause":[13],"life-threatening":[14],"pneumonia":[15],"other":[17],"severe":[18],"disease":[19],"manifestations":[20],"known":[21],"as":[22],"COVID-19.":[23],"This":[24,111,260],"health":[25],"crisis":[26],"resulted":[28],"in":[29,45,222,235,262,292,297],"a":[30,72,132,174,309],"significant":[31],"effort":[32],"stop":[34],"this":[38],"new":[39,55,325],"coronavirus.":[40],"However,":[41,171,234],"while":[42],"propagating":[43],"itself":[44],"human":[47,66,115],"population,":[48],"virus":[50],"accumulates":[51],"mutations":[52],"generates":[54],"variants":[56,319],"with":[57,162,207,264],"increased":[58],"fitness":[59],"ability":[62],"escape":[64,274],"immune":[67,91,201,220,273,281],"response.":[68],"Here":[69],"we":[70,172,303],"describe":[71],"color-based":[73],"barcoded":[74],"spike":[75,101],"flow":[76],"cytometric":[77],"assay":[78,112],"(BSFA)":[79],"that":[80,139,212,229,267,305],"is":[81,226,261,271],"particularly":[82],"useful":[83,310],"evaluate":[85,313],"directly":[87],"compare":[88],"humoral":[90,200,315],"response":[92,202,221,282,289],"directed":[93],"against":[94,317,328],"either":[95],"wild":[96],"type":[97],"(WT)":[98],"or":[99,104,127,131],"mutant":[100,128],"(S)":[102],"proteins":[103,130],"receptor-binding":[106],"domains":[107],"(RBD)":[108],"SARS-CoV-2.":[110,277],"employs":[113],"B":[116,157],"lymphoma":[117],"cell":[118,158],"line":[119,263],"Ramos,":[120],"transfected":[121],"for":[122,189],"stable":[123],"expression":[124,176],"WT":[126,151,238],"S":[129,152,258],"chimeric":[133,179],"RBD-CD8":[134,180],"fusion":[135],"protein.":[136,259],"We":[137],"find":[138,173],"alpha":[141,191,249],"beta":[143,193,256,269],"mutants":[144],"are":[145],"more":[146,284],"stably":[147],"expressed":[148],"than":[149,286,296],"protein":[153],"on":[154],"Ramos":[156],"surface":[159],"and/or":[160],"bind":[161,244],"higher":[163,291],"affinity":[164],"viral":[167],"entry":[168],"receptor":[169],"ACE2.":[170],"reduce":[175],"carrying":[181],"point":[183],"mutation":[184],"N501Y":[185],"E484K":[187],"characteristic":[188],"variant,":[194],"respectively.":[195],"comparison":[197,236],"12":[204,208],"vaccinated":[205,224,299],"probands":[206],"COVID-19":[209,294],"patients":[210,295],"shows":[211],"after":[213],"boost,":[215],"S-specific":[217],"IgG":[218,241,288],"class":[219,280],"group":[225],"similar":[227],"patient":[232],"group.":[233,300],"specific":[240],"serum":[242],"antibodies":[243],"less":[245],"well":[246],"variant":[250,257,270,275],"only":[252],"poorly":[253],"notion":[266],"an":[272],"IgA":[279],"was":[283],"variable":[285],"In":[301],"summary,":[302],"think":[304],"our":[306],"BSFA":[307],"represents":[308],"tool":[311],"immunity":[316],"emerging":[318],"analyze":[324],"vaccination":[326],"protocols":[327],"these":[329],"variants.":[330]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2021-10-11T00:00:00"}