{"id":"https://openalex.org/W3198643202","doi":"https://doi.org/10.3390/app11188314","title":"Extraction of ADP-Heptose and Kdo2-Lipid A from E. coli Deficient in the Heptosyltransferase I Gene","display_name":"Extraction of ADP-Heptose and Kdo2-Lipid A from E. coli Deficient in the Heptosyltransferase I Gene","publication_year":2021,"publication_date":"2021-09-08","ids":{"openalex":"https://openalex.org/W3198643202","doi":"https://doi.org/10.3390/app11188314","mag":"3198643202"},"language":"en","primary_location":{"id":"doi:10.3390/app11188314","doi":"https://doi.org/10.3390/app11188314","is_oa":true,"landing_page_url":"https://doi.org/10.3390/app11188314","pdf_url":"https://www.mdpi.com/2076-3417/11/18/8314/pdf?version=1631086719","source":{"id":"https://openalex.org/S4210205812","display_name":"Applied Sciences","issn_l":"2076-3417","issn":["2076-3417"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","norway-1","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/2076-3417/11/18/8314/pdf?version=1631086719","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5066445757","display_name":"Jozafina Milicaj","orcid":"https://orcid.org/0000-0002-6334-5941","observed_orcids":["https://orcid.org/0000-0002-6334-5941"]},"institutions":[{"id":"https://openalex.org/I100538780","display_name":"Wesleyan University","ror":"https://ror.org/05h7xva58","country_code":"US","type":"education","lineage":["https://openalex.org/I100538780"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jozafina Milicaj","raw_affiliation_strings":["Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA"],"raw_orcid":"https://orcid.org/0000-0002-6334-5941","affiliations":[{"raw_affiliation_string":"Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA","institution_ids":["https://openalex.org/I100538780"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088152942","display_name":"Colleen D. Castro","orcid":"https://orcid.org/0000-0002-4579-0339","observed_orcids":["https://orcid.org/0000-0002-4579-0339"]},"institutions":[{"id":"https://openalex.org/I100538780","display_name":"Wesleyan University","ror":"https://ror.org/05h7xva58","country_code":"US","type":"education","lineage":["https://openalex.org/I100538780"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Colleen D. Castro","raw_affiliation_strings":["Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA"],"raw_orcid":"https://orcid.org/0000-0002-4579-0339","affiliations":[{"raw_affiliation_string":"Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA","institution_ids":["https://openalex.org/I100538780"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064100237","display_name":"Nadiya Jaunbocus","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100538780","display_name":"Wesleyan University","ror":"https://ror.org/05h7xva58","country_code":"US","type":"education","lineage":["https://openalex.org/I100538780"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nadiya Jaunbocus","raw_affiliation_strings":["Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA","institution_ids":["https://openalex.org/I100538780"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019188545","display_name":"Erika Anne Taylor","orcid":"https://orcid.org/0000-0001-5598-640X","observed_orcids":["https://orcid.org/0000-0001-5598-640X"]},"institutions":[{"id":"https://openalex.org/I100538780","display_name":"Wesleyan University","ror":"https://ror.org/05h7xva58","country_code":"US","type":"education","lineage":["https://openalex.org/I100538780"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Erika A. Taylor","raw_affiliation_strings":["Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA"],"raw_orcid":"https://orcid.org/0000-0001-5598-640X","affiliations":[{"raw_affiliation_string":"Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA","institution_ids":["https://openalex.org/I100538780"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5019188545"],"corresponding_institution_ids":["https://openalex.org/I100538780"],"apc_list":{"value":2000,"currency":"CHF","value_usd":2160},"apc_paid":{"value":2000,"currency":"CHF","value_usd":2160},"fwci":0.3964,"has_fulltext":true,"cited_by_count":4,"citation_normalized_percentile":{"value":0.46623318,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":96,"max":97},"biblio":{"volume":"11","issue":"18","first_page":"8314","last_page":"8314"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.8999000191688538,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.8999000191688538,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.04349999874830246,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10943","display_name":"Escherichia coli research studies","score":0.017899999395012856,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adp-heptose","display_name":"ADP-heptose","score":0.9459999799728394},{"id":"https://openalex.org/keywords/kdo2-lipid-a","display_name":"Kdo2-lipid A","score":0.9850000143051147},{"id":"https://openalex.org/keywords/lipopolysaccharide-biosynthesis","display_name":"lipopolysaccharide biosynthesis","score":0.9399999976158142},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9269999861717224},{"id":"https://openalex.org/keywords/gene-disruption","display_name":"gene disruption","score":0.45899999141693115},{"id":"https://openalex.org/keywords/phenol-extraction","display_name":"phenol extraction","score":0.5640000104904175},{"id":"https://openalex.org/keywords/ultracentrifugation","display_name":"ultracentrifugation","score":0.7120000123977661},{"id":"https://openalex.org/keywords/gram-negative-infections","display_name":"gram-negative bacterial infection","score":0.656000018119812}],"concepts":[{"id":"https://openalex.org/C144464004","wikidata":"https://www.wikidata.org/wiki/Q3077338","display_name":"Lipid A","level":3,"score":0.76080322265625},{"id":"https://openalex.org/C2776621215","wikidata":"https://www.wikidata.org/wiki/Q424182","display_name":"Heptose","level":4,"score":0.6646077036857605},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6344107985496521},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5538583397865295},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4785221815109253},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.458112895488739},{"id":"https://openalex.org/C2777313579","wikidata":"https://www.wikidata.org/wiki/Q898365","display_name":"Glycosylation","level":2,"score":0.42536115646362305},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.41745099425315857},{"id":"https://openalex.org/C2778754761","wikidata":"https://www.wikidata.org/wiki/Q421804","display_name":"Lipopolysaccharide","level":2,"score":0.386372447013855},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3337748348712921},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.29199421405792236},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.16821131110191345},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.3390/app11188314","doi":"https://doi.org/10.3390/app11188314","is_oa":true,"landing_page_url":"https://doi.org/10.3390/app11188314","pdf_url":"https://www.mdpi.com/2076-3417/11/18/8314/pdf?version=1631086719","source":{"id":"https://openalex.org/S4210205812","display_name":"Applied Sciences","issn_l":"2076-3417","issn":["2076-3417"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","norway-1","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Sciences","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:94665a32d82e41588a5f921d7ee1dae1","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/94665a32d82e41588a5f921d7ee1dae1","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Applied Sciences, Vol 11, Iss 18, p 8314 (2021)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3390/app11188314","doi":"https://doi.org/10.3390/app11188314","is_oa":true,"landing_page_url":"https://doi.org/10.3390/app11188314","pdf_url":"https://www.mdpi.com/2076-3417/11/18/8314/pdf?version=1631086719","source":{"id":"https://openalex.org/S4210205812","display_name":"Applied Sciences","issn_l":"2076-3417","issn":["2076-3417"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","norway-1","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Sciences","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.428600013256073,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/6","score":0.699999988079071,"display_name":"Clean water and sanitation"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G5872260395","display_name":null,"funder_award_id":"1R15AI119907-01","funder_id":"https://openalex.org/F4320337544","funder_display_name":"Office of Extramural Research, National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320337544","display_name":"Office of Extramural Research, National Institutes of Health","ror":"https://ror.org/05aq6yn88"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3198643202.pdf","grobid_xml":"https://content.openalex.org/works/W3198643202.grobid-xml"},"referenced_works_count":35,"referenced_works":["https://openalex.org/W111685663","https://openalex.org/W1498580352","https://openalex.org/W1556311992","https://openalex.org/W1634828361","https://openalex.org/W1968820299","https://openalex.org/W2007331931","https://openalex.org/W2011683499","https://openalex.org/W2029224565","https://openalex.org/W2059013449","https://openalex.org/W2060534420","https://openalex.org/W2075814736","https://openalex.org/W2086902791","https://openalex.org/W2092278008","https://openalex.org/W2102528366","https://openalex.org/W2115494622","https://openalex.org/W2138882635","https://openalex.org/W2140663643","https://openalex.org/W2147891696","https://openalex.org/W2152311269","https://openalex.org/W2158253179","https://openalex.org/W2160711703","https://openalex.org/W2168016317","https://openalex.org/W2172150657","https://openalex.org/W2177149876","https://openalex.org/W2281081384","https://openalex.org/W2578503505","https://openalex.org/W2767158766","https://openalex.org/W2794330111","https://openalex.org/W2890043911","https://openalex.org/W2901083612","https://openalex.org/W2905026248","https://openalex.org/W2944088013","https://openalex.org/W3005408528","https://openalex.org/W4236273750","https://openalex.org/W4293048996"],"related_works":["https://openalex.org/W2254024421","https://openalex.org/W2059013449","https://openalex.org/W2172150657","https://openalex.org/W7166172642","https://openalex.org/W1634828361","https://openalex.org/W2911823606","https://openalex.org/W2106934132","https://openalex.org/W2102528366","https://openalex.org/W1598202930","https://openalex.org/W2063224703"],"abstract_inverted_index":{"The":[0],"enzymes":[1,27,70],"involved":[2,40],"in":[3,41,139,190,196,214,226,260],"lipopolysaccharide":[4],"(LPS)":[5],"biosynthesis,":[6],"including":[7,217],"Heptosyltransferase":[8,98],"I":[9,99],"(HepI),":[10],"are":[11,28,50,145,157,172,201,231],"critical":[12],"for":[13,31,151,174],"maintaining":[14],"the":[15,18,42,62,83,103,110,164,167,191,197,203,215,227,234,240,252,258,286],"integrity":[16],"of":[17,44,68,85,122,130,141,153,166,176,199,222,229,282,288],"bacterial":[19,37,76,91],"cell":[20],"wall,":[21],"and":[22,56,109,125,127,147,178,207,256,277],"therefore":[23],"these":[24,69,86,131,154],"LPS":[25,95],"biosynthetic":[26,96],"validated":[29],"targets":[30],"drug":[32],"discovery":[33],"to":[34,61,74,81,136,163,247,274],"treat":[35],"Gram-negative":[36],"infections.":[38],"Enzymes":[39],"biosynthesis":[43],"lipopolysaccharides":[45],"(LPSs)":[46],"utilize":[47],"substrates":[48,87,133],"that":[49,211],"synthetically":[51],"complex,":[52],"with":[53,78,149,254],"numerous":[54],"stereocenters":[55],"site-specific":[57],"glycosylation":[58],"patterns.":[59],"Due":[60],"relatively":[63],"complex":[64],"substrate":[65],"structures,":[66],"characterization":[67,117,287],"has":[71],"necessitated":[72],"strategies":[73],"generate":[75],"cells":[77,237],"gene":[79],"disruptions":[80],"enable":[82],"extraction":[84,152,175,198,228],"from":[88,182],"large":[89],"scale":[90],"growths.":[92],"Like":[93],"many":[94],"enzymes,":[97],"binds":[100],"two":[101,132],"substrates:":[102],"sugar":[104,111],"acceptor":[105],"substrate,":[106,113],"Kdo2-Lipid":[107,123,179],"A,":[108],"donor":[112],"ADP-l-glycero-d-manno-heptose":[114],"(ADPH).":[115],"HepI":[116],"experiments":[118],"require":[119],"copious":[120],"amounts":[121],"A":[124,180],"ADPH,":[126],"unsuccessful":[128],"extractions":[129,283],"can":[134],"lead":[135],"serious":[137],"delays":[138],"collection":[140],"data.":[142],"While":[143],"there":[144],"papers":[146],"theses":[148],"protocols":[150,171,272],"substrates,":[155],"they":[156],"often":[158],"missing":[159],"small":[160],"details":[161],"essential":[162],"success":[165,189,280],"extraction.":[168],"Herein":[169],"detailed":[170],"given":[173],"ADPH":[177,200],"(KLA)":[181],"E.":[183],"coli,":[184],"which":[185],"have":[186],"had":[187],"proven":[188],"Taylor":[192],"lab.":[193],"Key":[194,224],"steps":[195,225],"clearing":[202],"extract":[204],"through":[205],"ultracentrifugation":[206],"keeping":[208],"all":[209],"water":[210],"touches":[212],"anything":[213],"extraction,":[216,241],"filters,":[218],"at":[219],"a":[220,268,278],"pH":[221],"8.0.":[223],"KLA":[230,259],"properly":[232],"lysing":[233],"dried":[235],"down":[236],"before":[238],"starting":[239],"maximizing":[242],"yield":[243,276],"by":[244],"allowing":[245],"precipitate":[246],"form":[248],"overnight,":[249],"appropriately":[250],"washing":[251],"pellet":[253],"phenol":[255],"dissolving":[257],"1%":[261],"TEA":[262],"using":[263],"visual":[264],"cues,":[265],"rather":[266],"than":[267],"specific":[269],"volume.":[270],"These":[271],"led":[273],"increased":[275],"higher":[279],"rate":[281],"thereby":[284],"enabling":[285],"HepI.":[289]},"counts_by_year":[{"year":2022,"cited_by_count":4}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2021-09-13T00:00:00"}