{"id":"https://openalex.org/W3196571805","doi":"https://doi.org/10.1055/s-0041-1728194","title":"In vitro characterization of K5A and K5R variants of Factor IX","display_name":"In vitro characterization of K5A and K5R variants of Factor IX","publication_year":2021,"publication_date":"2021-06-01","ids":{"openalex":"https://openalex.org/W3196571805","doi":"https://doi.org/10.1055/s-0041-1728194","mag":"3196571805"},"language":"en","primary_location":{"id":"doi:10.1055/s-0041-1728194","doi":"https://doi.org/10.1055/s-0041-1728194","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0041-1728194","pdf_url":null,"source":{"id":"https://openalex.org/S60043848","display_name":"H\u00e4mostaseologie","issn_l":"0720-9355","issn":["0720-9355","2567-5761"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"H\u00e4mostaseologie","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112052418","display_name":"Shirim Machado","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S Knoll Machado","raw_affiliation_strings":["R&D, CSL Behring GmbH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"R&D, CSL Behring GmbH","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052386387","display_name":"T Kraushaar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Kraushaar","raw_affiliation_strings":["R&D, CSL Behring GmbH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"R&D, CSL Behring GmbH","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111715744","display_name":"John Roder","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J R\u00f6der","raw_affiliation_strings":["R&D, CSL Behring GmbH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"R&D, CSL Behring GmbH","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065177429","display_name":"Philipp Claar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P Claar","raw_affiliation_strings":["R&D, CSL Behring GmbH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"R&D, CSL Behring GmbH","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"C Hardy","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C Hardy","raw_affiliation_strings":["R&D, CSL Behring GmbH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"R&D, CSL Behring GmbH","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041926668","display_name":"MW Nolte","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"MW Nolte","raw_affiliation_strings":["R&D, CSL Behring GmbH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"R&D, CSL Behring GmbH","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064098844","display_name":"Sabine Pestel","orcid":"https://orcid.org/0000-0003-0999-1915","observed_orcids":["https://orcid.org/0000-0003-0999-1915"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S Pestel","raw_affiliation_strings":["R&D, CSL Behring GmbH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"R&D, CSL Behring GmbH","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068605002","display_name":"Michael Bacher","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"M Bacher","raw_affiliation_strings":["Institute of Immunology, Philipps University Marburg"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Immunology, Philipps University Marburg","institution_ids":["https://openalex.org/I161103922"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009966155","display_name":"Padmapriya Ponnuswamy","orcid":"https://orcid.org/0000-0001-6941-8796","observed_orcids":["https://orcid.org/0000-0001-6941-8796"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P Ponnuswamy","raw_affiliation_strings":["R&D, CSL Behring GmbH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"R&D, CSL Behring GmbH","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.24074074,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11280","display_name":"Hemophilia Treatment and Research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11280","display_name":"Hemophilia Treatment and Research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11917","display_name":"Coagulation, Bradykinin, Polyphosphates, and Angioedema","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10746","display_name":"Platelet Disorders and Treatments","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/factor-ix","display_name":"factor IX","score":0.8209999799728394},{"id":"https://openalex.org/keywords/extracellular-matrix-binding","display_name":"extracellular matrix binding","score":0.9259999990463257},{"id":"https://openalex.org/keywords/tenase-complex","display_name":"tenase complex","score":0.746999979019165},{"id":"https://openalex.org/keywords/clotting-activity","display_name":"clotting activity","score":0.6740000247955322},{"id":"https://openalex.org/keywords/recombinant-factor-ix","display_name":"recombinant factor IX","score":0.8180000185966492},{"id":"https://openalex.org/keywords/albumin-fusion-protein","display_name":"albumin fusion protein","score":0.5789999961853027},{"id":"https://openalex.org/keywords/michaelis-constant","display_name":"Michaelis constant","score":0.5},{"id":"https://openalex.org/keywords/surface-plasmon-resonance-binding","display_name":"surface plasmon resonance binding","score":0.7149999737739563},{"id":"https://openalex.org/keywords/hemophilia","display_name":"hemophilia","score":0.6980000138282776}],"concepts":[{"id":"https://openalex.org/C2777468819","wikidata":"https://www.wikidata.org/wiki/Q173670","display_name":"Arginine","level":3,"score":0.773776650428772},{"id":"https://openalex.org/C2776016237","wikidata":"https://www.wikidata.org/wiki/Q178430","display_name":"Lysine","level":3,"score":0.7404405474662781},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.6077011227607727},{"id":"https://openalex.org/C2781221834","wikidata":"https://www.wikidata.org/wiki/Q425360","display_name":"Factor IX","level":2,"score":0.6062765121459961},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5382359623908997},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5333393216133118},{"id":"https://openalex.org/C2778382381","wikidata":"https://www.wikidata.org/wiki/Q179217","display_name":"Coagulation","level":2,"score":0.4842798113822937},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4178951680660248},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.37799686193466187},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.35101813077926636},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3221256136894226},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22529557347297668},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10715830326080322},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.07208505272865295}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1055/s-0041-1728194","doi":"https://doi.org/10.1055/s-0041-1728194","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0041-1728194","pdf_url":null,"source":{"id":"https://openalex.org/S60043848","display_name":"H\u00e4mostaseologie","issn_l":"0720-9355","issn":["0720-9355","2567-5761"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"H\u00e4mostaseologie","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.6240000128746033}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4405048237","https://openalex.org/W1985872523","https://openalex.org/W4376105481","https://openalex.org/W6906446350","https://openalex.org/W4362521366","https://openalex.org/W4310113756","https://openalex.org/W7204525128","https://openalex.org/W4413116451","https://openalex.org/W2093444214","https://openalex.org/W1994839642"],"abstract_inverted_index":{"Objective":[0],"Recent":[1],"studies":[2],"indicate":[3],"that":[4,28,217,308],"binding":[5,30,298,313],"of":[6,32,67,105,136,167,228,252,284,300,315],"coagulation":[7],"factor":[8],"IX":[9],"(FIX)":[10],"to":[11,16,34,38,50,59,74,82,170,188,256,293,303],"extracellular":[12],"matrix":[13],"(ECM)":[14],"contributes":[15],"its":[17,22],"extravascular":[18],"(EV)":[19],"storage":[20],"and":[21,53,100,118,156,202,230,238,265,288],"hemostatic":[23],"efficacy.":[24],"It":[25],"is":[26,48,57],"postulated":[27],"the":[29,64,106,127,157,181,196,215,218,243,281,285,312,318],"affinity":[31],"FIX":[33,68,113,122,192,316],"ECM,":[35],"more":[36],"specifically":[37],"Collagen":[39],"IV":[40],"(Col-IV),":[41],"increases":[42],"when":[43,55],"Lysine":[44,56],"at":[45,272],"position":[46],"5":[47],"mutated":[49,58],"Arginine":[51],"(K5R)":[52],"decreases":[54],"Alanine":[60],"(K5A).":[61],"To":[62],"understand":[63],"physiological":[65],"role":[66],"in":[69,80,111,119,130,317],"EV":[70],"space":[71],"we":[72],"aimed":[73],"characterize":[75],"recombinantly":[76],"generated":[77],"K5":[78,107,178,247,270,286],"variants":[79,92,108,138,179,248,271,287],"comparison":[81],"wildtype":[83],"rFIX":[84,91,137,168,266,302],"molecules.":[85],"We":[86],"used":[87,162],"albumin":[88],"fused":[89],"(FP)":[90],"(rFIX-FP,":[93],"K5A-FP,":[94],"K5R-FP)":[95],"for":[96],"this":[97],"purpose.":[98],"Material":[99],"Methods":[101],"A":[102],"functional":[103],"characterization":[104],"was":[109,139,161,172,275],"conducted":[110],"a":[112,120,142,151],"one":[114],"stage":[115],"clotting":[116,193,222],"assay":[117],"modified":[121],"chromogenic":[123,144],"activity":[124,135,220,223,254],"assay,":[125],"mimicking":[126],"tenase":[128,197],"complex":[129,198],"vitro.":[131],"In":[132,195],"addition,":[133],"enzymatic":[134,253,291],"characterized":[140],"using":[141],"FIXa-specific":[143],"substrate.":[145],"Both":[146],"characterizations":[147],"were":[148],"analyzed":[149],"on":[150,297],"Michaelis-Menten":[152],"kinetic":[153,158],"based":[154],"model,":[155],"parameter":[159],"Km":[160,207],"as":[163,311],"key":[164],"comparator.":[165],"Binding":[166],"molecules":[169],"Col-IV":[171,264,304,310],"measured":[173],"by":[174],"SPR.":[175],"Results":[176],"All":[177,331],"decreased":[180],"clot":[182],"formation":[183],"time":[184],"substantially":[185],"from":[186],"76":[187],"37-38":[189],"seconds":[190],"(\u22481IU/mL":[191],"activity).":[194],"both":[199],"mutants":[200],"K5A-FP":[201,231],"K5R-FP":[203,229],"showed":[204,249,280],"an":[205],"identical":[206],"(0.20":[208],"nM).":[209],"These":[210],"results":[211,307],"are":[212],"consistent":[213],"with":[214],"observation":[216],"specific":[219,234,245],"(FIX":[221],"per":[224],"total":[225],"protein":[226],"amount)":[227],"displayed":[232],"similar":[233],"activities,":[235],"60":[236],"IU/mg":[237],"59":[239],"IU/mg,":[240],"respectively.":[241],"For":[242],"FIXa":[244],"substrate,":[246],"comparable":[250,290],"rates":[251],"relative":[255],"wildtype.":[257,294],"Finally,":[258],"no":[259],"detectable":[260],"interaction":[261],"between":[262],"immobilized":[263],"or":[267,269],"rFIX-FP":[268],"0.2":[273],"\u00b5M":[274],"observed.":[276],"Conclusion":[277],"Our":[278],"experiments":[279],"structural":[282],"integrity":[283],"their":[289],"characteristics":[292],"In-house":[295],"data":[296],"properties":[299],"these":[301],"contradicts":[305],"published":[306],"posit":[309],"partner":[314],"EVS.":[319],"Publication":[320,322],"History":[321],"Date:":[323],"18":[324],"June":[325],"2021":[326],"(online)":[327],"\u00a9":[328],"2021.":[329],"Thieme.":[330],"rights":[332],"reserved.":[333],"Georg":[334],"Thieme":[335],"Verlag":[336],"KG":[337],"R\u00fcdigerstra\u00dfe":[338],"14,":[339],"70469":[340],"Stuttgart,":[341],"Germany":[342]},"counts_by_year":[],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2021-09-13T00:00:00"}
