{"id":"https://openalex.org/W3190944854","doi":"https://doi.org/10.17504/protocols.io.kracv2e","title":"Immunofluorescence for free-floating brain sections v1","display_name":"Immunofluorescence for free-floating brain sections v1","publication_year":2017,"publication_date":"2017-11-09","ids":{"openalex":"https://openalex.org/W3190944854","doi":"https://doi.org/10.17504/protocols.io.kracv2e","mag":"3190944854"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.kracv2e","doi":"https://doi.org/10.17504/protocols.io.kracv2e","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.kracv2e","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.17504/protocols.io.kracv2e","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074225272","display_name":"Jill R. Crittenden","orcid":"https://orcid.org/0000-0002-1981-1917","observed_orcids":["https://orcid.org/0000-0002-1981-1917"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jill R. Crittenden","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103221437","display_name":"Tomoko Yoshida","orcid":"https://orcid.org/0000-0003-3961-0095","observed_orcids":["https://orcid.org/0000-0003-3961-0095"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Tomoko Yoshida","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Margaret I. Davis","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Margaret I. Davis","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5081686751","display_name":"Ann M. Graybiel","orcid":"https://orcid.org/0000-0002-4326-7720","observed_orcids":["https://orcid.org/0000-0002-4326-7720"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ann M. Graybiel","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.6338000297546387,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.6338000297546387,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.2012999951839447,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14134","display_name":"Medical and Biological Sciences","score":0.048700001090765,"subfield":{"id":"https://openalex.org/subfields/2702","display_name":"Anatomy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/immunofluorescence","display_name":"immunofluorescence","score":0.9760000109672546},{"id":"https://openalex.org/keywords/brain-sections","display_name":"brain sections","score":0.8489999771118164},{"id":"https://openalex.org/keywords/tissue-fixation","display_name":"tissue fixation","score":0.8259999752044678},{"id":"https://openalex.org/keywords/brain-tissue-preparation","display_name":"brain tissue preparation","score":0.5130000114440918}],"concepts":[{"id":"https://openalex.org/C2778236600","wikidata":"https://www.wikidata.org/wiki/Q592324","display_name":"Immunofluorescence","level":3,"score":0.6526808142662048},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1443222463130951},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.11243000626564026},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.05160480737686157}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.kracv2e","doi":"https://doi.org/10.17504/protocols.io.kracv2e","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.kracv2e","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.kracv2e","doi":"https://doi.org/10.17504/protocols.io.kracv2e","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.kracv2e","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4409759133","https://openalex.org/W4393303300","https://openalex.org/W4389429210","https://openalex.org/W4413392627","https://openalex.org/W4295350592","https://openalex.org/W4409540340","https://openalex.org/W4409699123","https://openalex.org/W4406707196","https://openalex.org/W4394000983","https://openalex.org/W7221575169"],"abstract_inverted_index":{"This":[0],"protocol":[1],"describes":[2],"how":[3],"to":[4],"prepare":[5],"fixed":[6],"brain":[7],"sections":[8],"and":[9],"process":[10],"them":[11],"for":[12],"immunofluorescence.":[13]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2021-08-16T00:00:00"}