{"id":"https://openalex.org/W3190572311","doi":"https://doi.org/10.1002/ece3.7788","title":"Method for quick DNA barcode reference library construction","display_name":"Method for quick DNA barcode reference library construction","publication_year":2021,"publication_date":"2021-08-04","ids":{"openalex":"https://openalex.org/W3190572311","doi":"https://doi.org/10.1002/ece3.7788","mag":"3190572311","pmid":"https://pubmed.ncbi.nlm.nih.gov/34522329","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8427591"},"language":"en","primary_location":{"id":"doi:10.1002/ece3.7788","doi":"https://doi.org/10.1002/ece3.7788","is_oa":true,"landing_page_url":"https://doi.org/10.1002/ece3.7788","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ece3.7788","source":{"id":"https://openalex.org/S2482021738","display_name":"Ecology and Evolution","issn_l":"2045-7758","issn":["2045-7758"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Ecology and Evolution","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ece3.7788","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074537749","display_name":"Yanlei Liu","orcid":"https://orcid.org/0000-0002-8160-0141","observed_orcids":["https://orcid.org/0000-0002-8160-0141"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210158097","display_name":"Institute of Botany","ror":"https://ror.org/05hr3ch11","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097"]},{"id":"https://openalex.org/I4210165038","display_name":"University of Chinese Academy of Sciences","ror":"https://ror.org/05qbk4x57","country_code":"CN","type":"education","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210165038"]},{"id":"https://openalex.org/I4391767923","display_name":"State Key Laboratory of Systematic and Evolutionary Botany","ror":"https://ror.org/05wq99a66","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yanlei Liu","raw_affiliation_strings":["College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China","State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China"],"raw_orcid":"https://orcid.org/0000-0002-8160-0141","affiliations":[{"raw_affiliation_string":"College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210165038"]},{"raw_affiliation_string":"State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101440217","display_name":"Chao Xu","orcid":"https://orcid.org/0000-0002-9678-4772","observed_orcids":["https://orcid.org/0000-0002-9678-4772"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210158097","display_name":"Institute of Botany","ror":"https://ror.org/05hr3ch11","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097"]},{"id":"https://openalex.org/I4391767923","display_name":"State Key Laboratory of Systematic and Evolutionary Botany","ror":"https://ror.org/05wq99a66","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chao Xu","raw_affiliation_strings":["State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080744549","display_name":"Yuzhe Sun","orcid":"https://orcid.org/0000-0002-1891-7883","observed_orcids":["https://orcid.org/0000-0002-1891-7883"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210158097","display_name":"Institute of Botany","ror":"https://ror.org/05hr3ch11","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097"]},{"id":"https://openalex.org/I4210165038","display_name":"University of Chinese Academy of Sciences","ror":"https://ror.org/05qbk4x57","country_code":"CN","type":"education","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210165038"]},{"id":"https://openalex.org/I4391767923","display_name":"State Key Laboratory of Systematic and Evolutionary Botany","ror":"https://ror.org/05wq99a66","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yuzhe Sun","raw_affiliation_strings":["College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China","State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210165038"]},{"raw_affiliation_string":"State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100451597","display_name":"Xun Chen","orcid":"https://orcid.org/0009-0003-8671-5217","observed_orcids":["https://orcid.org/0009-0003-8671-5217","https://orcid.org/0000-0001-7467-8066"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210158097","display_name":"Institute of Botany","ror":"https://ror.org/05hr3ch11","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097"]},{"id":"https://openalex.org/I4391767923","display_name":"State Key Laboratory of Systematic and Evolutionary Botany","ror":"https://ror.org/05wq99a66","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]},{"id":"https://openalex.org/I47689461","display_name":"Northeast Forestry University","ror":"https://ror.org/02yxnh564","country_code":"CN","type":"education","lineage":["https://openalex.org/I47689461"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xun Chen","raw_affiliation_strings":["College of Landscape Architecture, Northeast Forestry University, Harbin, China","State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Landscape Architecture, Northeast Forestry University, Harbin, China","institution_ids":["https://openalex.org/I47689461"]},{"raw_affiliation_string":"State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033665614","display_name":"Wenpan Dong","orcid":"https://orcid.org/0000-0002-8371-3246","observed_orcids":["https://orcid.org/0000-0002-8371-3246"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I31683504","display_name":"Beijing Forestry University","ror":"https://ror.org/04xv2pc41","country_code":"CN","type":"education","lineage":["https://openalex.org/I1327237609","https://openalex.org/I31683504","https://openalex.org/I4210127390"]},{"id":"https://openalex.org/I4210158097","display_name":"Institute of Botany","ror":"https://ror.org/05hr3ch11","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097"]},{"id":"https://openalex.org/I4391767923","display_name":"State Key Laboratory of Systematic and Evolutionary Botany","ror":"https://ror.org/05wq99a66","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wenpan Dong","raw_affiliation_strings":["Laboratory of Systematic Evolution and Biogeography of Woody Plants, School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China","State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Systematic Evolution and Biogeography of Woody Plants, School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China","institution_ids":["https://openalex.org/I31683504"]},{"raw_affiliation_string":"State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102417515","display_name":"Xueying Yang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1302611135","display_name":"Ministry of Public Security of the People's Republic of China","ror":"https://ror.org/00bt9we26","country_code":"CN","type":"government","lineage":["https://openalex.org/I1302611135"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Xueying Yang","raw_affiliation_strings":["National Engineering Laboratory for Forensic Science, Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Engineering Laboratory for Forensic Science, Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, China","institution_ids":["https://openalex.org/I1302611135"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5014924327","display_name":"Shiliang Zhou","orcid":"https://orcid.org/0000-0001-5013-5497","observed_orcids":["https://orcid.org/0000-0001-5013-5497"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210158097","display_name":"Institute of Botany","ror":"https://ror.org/05hr3ch11","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097"]},{"id":"https://openalex.org/I4210165038","display_name":"University of Chinese Academy of Sciences","ror":"https://ror.org/05qbk4x57","country_code":"CN","type":"education","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210165038"]},{"id":"https://openalex.org/I4391767923","display_name":"State Key Laboratory of Systematic and Evolutionary Botany","ror":"https://ror.org/05wq99a66","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Shiliang Zhou","raw_affiliation_strings":["College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China","State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210165038"]},{"raw_affiliation_string":"State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4391767923"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":7,"corresponding_author_ids":["https://openalex.org/A5014924327","https://openalex.org/A5102417515"],"corresponding_institution_ids":["https://openalex.org/I1302611135","https://openalex.org/I19820366","https://openalex.org/I4210158097","https://openalex.org/I4210165038","https://openalex.org/I4391767923"],"apc_list":{"value":2200,"currency":"USD","value_usd":2200},"apc_paid":{"value":2200,"currency":"USD","value_usd":2200},"fwci":3.0008,"has_fulltext":true,"cited_by_count":28,"citation_normalized_percentile":{"value":0.91522231,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"11","issue":"17","first_page":"11627","last_page":"11638"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.9603999853134155,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.9603999853134155,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.020099999383091927,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10385","display_name":"Plant Diversity and Evolution","score":0.006899999920278788,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-barcoding","display_name":"DNA barcoding","score":0.9959999918937683},{"id":"https://openalex.org/keywords/plant-identification","display_name":"plant identification","score":0.9139999747276306},{"id":"https://openalex.org/keywords/its1","display_name":"ITS1","score":0.4490000009536743},{"id":"https://openalex.org/keywords/its2","display_name":"ITS2","score":1.0},{"id":"https://openalex.org/keywords/matk","display_name":"matK","score":0.984000027179718},{"id":"https://openalex.org/keywords/rbcl-gene","display_name":"rbcL","score":0.996999979019165},{"id":"https://openalex.org/keywords/sequencing-technology-comparison","display_name":"sequencing platform comparison","score":0.6869999766349792},{"id":"https://openalex.org/keywords/sanger-sequencing","display_name":"Sanger sequencing","score":0.9120000004768372},{"id":"https://openalex.org/keywords/flowering-plants","display_name":"flowering plants","score":0.5429999828338623},{"id":"https://openalex.org/keywords/species-coverage","display_name":"species coverage","score":0.49900001287460327}],"concepts":[{"id":"https://openalex.org/C141732470","wikidata":"https://www.wikidata.org/wiki/Q1154642","display_name":"DNA barcoding","level":2,"score":0.8708735704421997},{"id":"https://openalex.org/C2776841711","wikidata":"https://www.wikidata.org/wiki/Q856","display_name":"Barcode","level":2,"score":0.8585875034332275},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.68435138463974},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6197495460510254},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6083975434303284},{"id":"https://openalex.org/C164928082","wikidata":"https://www.wikidata.org/wiki/Q6063435","display_name":"Ion semiconductor sequencing","level":4,"score":0.5990270972251892},{"id":"https://openalex.org/C76818968","wikidata":"https://www.wikidata.org/wiki/Q181940","display_name":"Sanger sequencing","level":4,"score":0.5842482447624207},{"id":"https://openalex.org/C106337198","wikidata":"https://www.wikidata.org/wiki/Q13564012","display_name":"Illumina dye sequencing","level":4,"score":0.42672795057296753},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.40790241956710815},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.373540997505188},{"id":"https://openalex.org/C77088390","wikidata":"https://www.wikidata.org/wiki/Q8513","display_name":"Database","level":1,"score":0.33691054582595825},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2868502140045166},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.21035602688789368},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.1002/ece3.7788","doi":"https://doi.org/10.1002/ece3.7788","is_oa":true,"landing_page_url":"https://doi.org/10.1002/ece3.7788","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ece3.7788","source":{"id":"https://openalex.org/S2482021738","display_name":"Ecology and Evolution","issn_l":"2045-7758","issn":["2045-7758"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Ecology and Evolution","raw_type":"journal-article"},{"id":"pmid:34522329","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34522329","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Ecology and evolution","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8427591","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8427591/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Ecol Evol","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:d72b8dc7ddbf41518f364e9ee4f45cab","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/d72b8dc7ddbf41518f364e9ee4f45cab","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Ecology and Evolution, Vol 11, Iss 17, Pp 11627-11638 (2021)","raw_type":"article"},{"id":"pmh:oai:ir.ibcas.ac.cn:2S10CLM1/26312","doi":null,"is_oa":false,"landing_page_url":"http://ir.ibcas.ac.cn/handle/2S10CLM1/26312","pdf_url":null,"source":{"id":"https://openalex.org/S7407064337","display_name":"Institute of Botany, CAS Institutional Repository","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Article"}],"best_oa_location":{"id":"doi:10.1002/ece3.7788","doi":"https://doi.org/10.1002/ece3.7788","is_oa":true,"landing_page_url":"https://doi.org/10.1002/ece3.7788","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ece3.7788","source":{"id":"https://openalex.org/S2482021738","display_name":"Ecology and Evolution","issn_l":"2045-7758","issn":["2045-7758"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Ecology and Evolution","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1373841872","display_name":null,"funder_award_id":"NSFC31872679","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G7401049179","display_name":null,"funder_award_id":"XDA19050303","funder_id":"https://openalex.org/F4320321133","funder_display_name":"Chinese Academy of Sciences"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320321133","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35"},{"id":"https://openalex.org/F4320326699","display_name":"Ministry of Public Security of the People's Republic of China","ror":"https://ror.org/00bt9we26"},{"id":"https://openalex.org/F4320330304","display_name":"Institute of Forensic Science, Ministry of Public Security","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3190572311.pdf","grobid_xml":"https://content.openalex.org/works/W3190572311.grobid-xml"},"referenced_works_count":68,"referenced_works":["https://openalex.org/W1660155968","https://openalex.org/W1877951127","https://openalex.org/W1968458420","https://openalex.org/W1975874700","https://openalex.org/W1996816368","https://openalex.org/W2014985379","https://openalex.org/W2020200409","https://openalex.org/W2021656021","https://openalex.org/W2026992598","https://openalex.org/W2039930928","https://openalex.org/W2042972043","https://openalex.org/W2047669659","https://openalex.org/W2052447833","https://openalex.org/W2057536116","https://openalex.org/W2071510336","https://openalex.org/W2086378149","https://openalex.org/W2088833470","https://openalex.org/W2093296928","https://openalex.org/W2098035523","https://openalex.org/W2099901798","https://openalex.org/W2108718991","https://openalex.org/W2111536597","https://openalex.org/W2124715997","https://openalex.org/W2129971040","https://openalex.org/W2132543522","https://openalex.org/W2133058864","https://openalex.org/W2140078093","https://openalex.org/W2157107905","https://openalex.org/W2162667876","https://openalex.org/W2167406761","https://openalex.org/W2189103325","https://openalex.org/W2257034565","https://openalex.org/W2401404581","https://openalex.org/W2414074348","https://openalex.org/W2513506562","https://openalex.org/W2531474461","https://openalex.org/W2538509698","https://openalex.org/W2553482582","https://openalex.org/W2576417104","https://openalex.org/W2592367925","https://openalex.org/W2618969823","https://openalex.org/W2626813446","https://openalex.org/W2724140861","https://openalex.org/W2725502961","https://openalex.org/W2743198371","https://openalex.org/W2744432346","https://openalex.org/W2752511450","https://openalex.org/W2752948555","https://openalex.org/W2757805629","https://openalex.org/W2769882831","https://openalex.org/W2792034759","https://openalex.org/W2795121360","https://openalex.org/W2799524357","https://openalex.org/W2801835880","https://openalex.org/W2803457956","https://openalex.org/W2919896006","https://openalex.org/W2950453293","https://openalex.org/W2962534736","https://openalex.org/W2963276645","https://openalex.org/W2977654937","https://openalex.org/W2982671208","https://openalex.org/W2998955672","https://openalex.org/W3008860748","https://openalex.org/W3017323551","https://openalex.org/W3022965930","https://openalex.org/W3083045266","https://openalex.org/W4248181066","https://openalex.org/W6959921104"],"related_works":["https://openalex.org/W4307583995","https://openalex.org/W3094391910","https://openalex.org/W2607484964","https://openalex.org/W3028912213","https://openalex.org/W2765449108","https://openalex.org/W3136994990","https://openalex.org/W2340109007","https://openalex.org/W6977480413","https://openalex.org/W2189409557","https://openalex.org/W4396233908"],"abstract_inverted_index":{"Abstract":[0],"DNA":[1,61,115,175,182],"barcoding":[2,183],"has":[3],"become":[4],"one":[5],"of":[6,17,25,28,71],"the":[7,23,88,94,139,160,169],"most":[8],"important":[9],"techniques":[10],"in":[11,195],"plant":[12],"species":[13,30,188],"identification.":[14],"Successful":[15],"application":[16],"this":[18],"technology":[19],"is":[20,111,164,190],"dependent":[21],"on":[22,75],"availability":[24],"reference":[26,184],"database":[27],"high":[29,187],"coverage.":[31],"Unfortunately,":[32],"there":[33],"are":[34,148],"experimental":[35],"and":[36,69,83,87,101,134,155,159,171],"data":[37],"processing":[38],"challenges":[39],"to":[40,54,123,145,166,192],"construct":[41],"such":[42],"a":[43,46,119,196],"library":[44,185],"within":[45],"short":[47],"time.":[48],"Here,":[49],"we":[50,104],"present":[51],"our":[52,146],"solutions":[53],"these":[55],"challenges.":[56],"We":[57,117],"sequenced":[58],"six":[59],"conventional":[60,114],"barcode":[62],"fragments":[63],"(ITS1,":[64],"ITS2,":[65],"matK1,":[66],"matK2,":[67],"rbcL1,":[68],"rbcL2)":[70],"380":[72],"flowering":[73],"plants":[74],"next\u2010generation":[76],"sequencing":[77,90,95],"(NGS)":[78],"platforms":[79],"(Illumina":[80],"Hiseq":[81],"2500":[82],"Ion":[84],"Torrent":[85],"S5)":[86],"Sanger":[89],"platform.":[91],"After":[92],"comparing":[93],"depths,":[96],"read":[97],"lengths,":[98],"base":[99,102],"qualities,":[100],"accuracies,":[103],"conclude":[105],"that":[106],"Illumina":[107],"Hiseq2500":[108],"PE250":[109],"run":[110],"suitable":[112],"for":[113,130,174],"barcoding.":[116,176],"developed":[118],"new":[120],"\u201cCotu\u201d":[121],"method":[122,147],"create":[124],"consensus":[125],"sequences":[126,133],"from":[127],"NGS":[128],"reads":[129],"longer":[131],"output":[132],"more":[135],"reliable":[136],"bases":[137],"than":[138],"other":[140],"three":[141],"methods.":[142],"Step\u2010by\u2010step":[143],"instructions":[144],"provided.":[149],"By":[150],"using":[151],"high\u2010throughput":[152],"machines":[153],"(PCR":[154],"NGS),":[156],"labeling":[157],"PCR,":[158],"Cotu":[161],"method,":[162],"it":[163],"possible":[165],"significantly":[167],"reduce":[168],"cost":[170],"labor":[172],"investments":[173],"A":[177],"regional":[178],"or":[179],"even":[180],"global":[181],"with":[186],"coverage":[189],"likely":[191],"be":[193],"constructed":[194],"few":[197],"years.":[198]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":11},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2021-08-16T00:00:00"}