{"id":"https://openalex.org/W3190469712","doi":"https://doi.org/10.1097/ju.0000000000002020.01","title":"PD27-01 DEFINING SYNAPTOTAGMIN-1 AS THE RECEPTOR FOR BOTULINUM NEUROTOXIN B IN THE BLADDER","display_name":"PD27-01 DEFINING SYNAPTOTAGMIN-1 AS THE RECEPTOR FOR BOTULINUM NEUROTOXIN B IN THE BLADDER","publication_year":2021,"publication_date":"2021-08-04","ids":{"openalex":"https://openalex.org/W3190469712","doi":"https://doi.org/10.1097/ju.0000000000002020.01","mag":"3190469712"},"language":"en","primary_location":{"id":"doi:10.1097/ju.0000000000002020.01","doi":"https://doi.org/10.1097/ju.0000000000002020.01","is_oa":false,"landing_page_url":"https://doi.org/10.1097/ju.0000000000002020.01","pdf_url":null,"source":{"id":"https://openalex.org/S30525748","display_name":"The Journal of Urology","issn_l":"0022-5347","issn":["0022-5347","1527-3792"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Urology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5035230975","display_name":"Hatim Thaker","orcid":"https://orcid.org/0000-0002-0373-4267","observed_orcids":["https://orcid.org/0000-0002-0373-4267"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hatim Thaker","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100436657","display_name":"Jie Zhang","orcid":"https://orcid.org/0000-0001-8831-7464","observed_orcids":["https://orcid.org/0000-0001-8831-7464","https://orcid.org/0000-0001-6826-7367"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jie Zhang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069777569","display_name":"Shin-Ichiro Miyashita","orcid":"https://orcid.org/0000-0002-4815-9323","observed_orcids":["https://orcid.org/0000-0002-4815-9323"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shin-Ichiro Miyashita","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006501212","display_name":"Vivian Cristofaro","orcid":"https://orcid.org/0000-0002-4826-2021","observed_orcids":["https://orcid.org/0000-0002-4826-2021"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Vivian Cristofaro","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115590136","display_name":"Maryrose P. Sullivan","orcid":"https://orcid.org/0000-0001-6731-415X","observed_orcids":["https://orcid.org/0000-0001-6731-415X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Maryrose Sullivan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069789939","display_name":"Rosalyn M. Adam","orcid":"https://orcid.org/0000-0002-0943-6236","observed_orcids":["https://orcid.org/0000-0002-0943-6236"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Rosalyn Adam","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5086559528","display_name":"Min Dong","orcid":"https://orcid.org/0000-0002-1744-7293","observed_orcids":["https://orcid.org/0000-0002-1744-7293"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Min Dong","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3000,"currency":"USD","value_usd":3000},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.17848233,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"206","issue":"Supplement 3","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10279","display_name":"Urinary Bladder and Prostate Research","score":0.6395999789237976,"subfield":{"id":"https://openalex.org/subfields/2748","display_name":"Urology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10279","display_name":"Urinary Bladder and Prostate Research","score":0.6395999789237976,"subfield":{"id":"https://openalex.org/subfields/2748","display_name":"Urology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11274","display_name":"Botulinum Toxin and Related Neurological Disorders","score":0.34130001068115234,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11232","display_name":"Urinary Tract Infections Management","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/botulinum-neurotoxin-b","display_name":"botulinum neurotoxin B","score":0.859000027179718},{"id":"https://openalex.org/keywords/synaptotagmin-1","display_name":"synaptotagmin-1","score":0.9750000238418579},{"id":"https://openalex.org/keywords/synaptotagmin-2","display_name":"synaptotagmin 2","score":0.9959999918937683},{"id":"https://openalex.org/keywords/receptor-specificity","display_name":"receptor specificity","score":0.6690000295639038},{"id":"https://openalex.org/keywords/bladder","display_name":"urinary bladder","score":0.5550000071525574},{"id":"https://openalex.org/keywords/detrusor-overactivity","display_name":"detrusor overactivity","score":0.9879999756813049},{"id":"https://openalex.org/keywords/onabotulinumtoxina","display_name":"onabotulinumtoxinA","score":0.8119999766349792},{"id":"https://openalex.org/keywords/smooth-muscle","display_name":"smooth muscle","score":0.4950000047683716},{"id":"https://openalex.org/keywords/skeletal-muscle","display_name":"skeletal muscle","score":0.7960000038146973},{"id":"https://openalex.org/keywords/urothelium","display_name":"urothelium","score":0.5770000219345093},{"id":"https://openalex.org/keywords/lower-urinary-tract-dysfunction","display_name":"lower urinary tract dysfunction","score":0.503000020980835}],"concepts":[{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.7258967161178589},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.5319030284881592},{"id":"https://openalex.org/C2777478456","wikidata":"https://www.wikidata.org/wiki/Q208413","display_name":"Botulinum toxin","level":2,"score":0.46357303857803345},{"id":"https://openalex.org/C2778941218","wikidata":"https://www.wikidata.org/wiki/Q331231","display_name":"Overactive bladder","level":3,"score":0.4382244646549225},{"id":"https://openalex.org/C126894567","wikidata":"https://www.wikidata.org/wiki/Q105650","display_name":"Urology","level":1,"score":0.3616068959236145},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.34137171506881714},{"id":"https://openalex.org/C141071460","wikidata":"https://www.wikidata.org/wiki/Q40821","display_name":"Surgery","level":1,"score":0.2055450975894928},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.19508680701255798},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1097/ju.0000000000002020.01","doi":"https://doi.org/10.1097/ju.0000000000002020.01","is_oa":false,"landing_page_url":"https://doi.org/10.1097/ju.0000000000002020.01","pdf_url":null,"source":{"id":"https://openalex.org/S30525748","display_name":"The Journal of Urology","issn_l":"0022-5347","issn":["0022-5347","1527-3792"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Urology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3023898534","https://openalex.org/W3205080025","https://openalex.org/W4360607966","https://openalex.org/W142366136","https://openalex.org/W2132166776","https://openalex.org/W424190","https://openalex.org/W2981965744","https://openalex.org/W2076818171","https://openalex.org/W2000807287","https://openalex.org/W4207064017"],"abstract_inverted_index":{"You":[0],"have":[1,182],"accessJournal":[2],"of":[3,141,166,292,378,442,467],"UrologyUrodynamics/Lower":[4],"Urinary":[5],"Tract":[6],"Dysfunction/Female":[7],"Pelvic":[8],"Medicine:":[9],"Basic":[10],"Research":[11,472],"&":[12,505],"Pathophysiology":[13],"(PD27)1":[14],"Sep":[15],"2021PD27-01":[16],"DEFINING":[17],"SYNAPTOTAGMIN-1":[18],"AS":[19],"THE":[20,27],"RECEPTOR":[21],"FOR":[22],"BOTULINUM":[23],"NEUROTOXIN":[24],"B":[25],"IN":[26],"BLADDER":[28],"Hatim":[29,44,517],"Thaker,":[30],"Jie":[31,53,524],"Zhang,":[32],"Shin-Ichiro":[33,62,531],"Miyashita,":[34],"Vivian":[35,71,538],"Cristofaro,":[36],"Maryrose":[37,80,545],"Sullivan,":[38],"Rosalyn":[39,89,552],"Adam,":[40],"and":[41,98,123,178,212,219,247,256,265,327,364,388,407,459,475,483,494,513],"Min":[42,99,559],"Dong":[43,101,560],"ThakerHatim":[45],"Thaker":[46,518],"More":[47,56,65,74,83,92,102,519,526,533,540,547,554,561],"articles":[48,57,66,75,84,93,103,520,527,534,541,548,555,562],"by":[49,58,67,76,85,94,104,238,489,508,521,528,535,542,549,556,563],"this":[50,59,68,77,86,95,105,522,529,536,543,550,557,564],"author":[51,60,69,78,87,96,106,523,530,537,544,551,558,565],",":[52,61,70,79,88,97],"ZhangJie":[54],"Zhang":[55,525],"MiyashitaShin-Ichiro":[63],"Miyashita":[64,532],"CristofaroVivian":[72],"Cristofaro":[73,539],"SullivanMaryrose":[81],"Sullivan":[82,546],"AdamRosalyn":[90],"Adam":[91,553],"DongMin":[100],"View":[107],"All":[108,567],"Author":[109],"Informationhttps://doi.org/10.1097/JU.0000000000002020.01AboutPDF":[110],"ToolsAdd":[111],"to":[112,145,159,201,217,267,310,323,332,384,449,452,479,485],"favoritesDownload":[113],"CitationsTrack":[114],"CitationsPermissionsReprints":[115],"ShareFacebookLinked":[116],"InTwitterEmail":[117],"Abstract":[118],"INTRODUCTION":[119],"AND":[120],"OBJECTIVE:":[121],"Neurogenic":[122],"idiopathic":[124],"detrusor":[125],"overactivity":[126],"(DO)":[127],"is":[128,150,429],"currently":[129],"treated":[130],"with":[131,168,263,269,351],"onabotulinumtoxin":[132],"A":[133],"(BoNT/A)":[134],"as":[135,156,185],"third":[136],"line":[137],"therapy.":[138],"In":[139,395],"cases":[140],"treatment":[142],"failure,":[143],"alternatives":[144],"BoNT/A":[146],"are":[147],"limited.":[148],"BoNT/B":[149,161,186,208,221,251,293,334,354,379,397,415,435,451],"another":[151],"serotype":[152],"that":[153,427],"could":[154],"serve":[155],"an":[157,447],"alternative":[158],"BoNT/A.":[160],"shares":[162],"the":[163,189,203,229,430],"same":[164],"mode":[165],"action":[167,209,313],"BoNT/A,":[169],"but":[170,367],"uses":[171],"different":[172],"receptors.":[173,187],"Two":[174],"homologous":[175],"proteins,":[176],"synaptotagmin-1":[177],"-2":[179],"(Syt-1,":[180],"-2),":[181],"been":[183],"identified":[184],"However,":[188],"dominant":[190],"receptor":[191,227,249,433],"form":[192],"in":[193,210,223,228,287,296,335,359,362,386,401,405,436],"bladder":[194,290,315,376,437,457],"tissues":[195,342,458],"remains":[196],"unknown.":[197],"Here,":[198],"we":[199],"aim":[200],"determine":[202],"specific":[204,226],"Syt":[205],"responsible":[206],"for":[207,434],"skeletal":[211,340,403,464],"smooth":[213,284],"muscle,":[214],"allowing":[215],"us":[216],"engineer":[218,450],"improve":[220,453],"efficacy":[222,455],"recognizing":[224],"its":[225,454,461],"bladder.":[230],"METHODS:":[231],"Transgenic":[232],"knock-in":[233],"(KI)":[234],"mice":[235,304,391,413],"were":[236,260],"generated":[237],"CRISPR/Cas9":[239],"approach,":[240],"where":[241],"a":[242,344,356],"triple-residue":[243],"mutation":[244],"on":[245,314,456,463],"Syt-1":[246,324,328,369,408,428,443],"Syt-2":[248,345,365,389,411,445],"abolishes":[250],"binding.":[252],"Wild":[253],"type":[254],"(WT)":[255],"KI":[257,329,390,409,412],"mouse":[258],"bladders":[259,319,330,371],"harvested,":[261],"incubated":[262],"BoNT/B,":[264],"subjected":[266],"immunostaining":[268],"confocal":[270],"microscopy":[271],"or":[272],"ex":[273],"vivo":[274,348],"nerve-evoked":[275,360],"contraction":[276,349],"studies":[277,350],"via":[278,299],"electrical":[279],"field":[280],"stimulation.":[281],"To":[282],"evaluate":[283],"muscle":[285,341],"response":[286],"vivo,":[288],"intramural":[289],"injection":[291,377],"(3.25":[294],"pg":[295],"5":[297],"uL)":[298],"laparotomy":[300],"was":[301],"done.":[302],"Post-op,":[303],"underwent":[305],"voiding":[306],"spot":[307],"assay":[308],"(VSA)":[309],"assess":[311],"toxin":[312],"function.":[316],"RESULTS:":[317],"WT":[318,363,387,406],"demonstrated":[320],"high":[321],"immunoreactivity":[322],"(Panel":[325,337,372,393,422],"A),":[326],"failed":[331],"bind":[333],"vitro":[336],"B).":[338],"Diaphragm":[339],"showed":[343,355],"predominance.":[346],"Ex":[347],"0.3":[352],"nM":[353],"90%":[357],"reduction":[358],"contractions":[361],"KI,":[366,370],"not":[368],"D).":[373],"VSA":[374],"after":[375],"reduced":[380],"voided":[381],"volumes,":[382],"akin":[383],"retention,":[385],"only":[392],"E).":[394],"contrast,":[396],"produced":[398],"full":[399],"paralysis":[400,421],"hindlimb":[402],"muscles":[404],"mice;":[410],"tolerated":[414],"at":[416],"30x":[417],"higher":[418],"doses":[419],"without":[420],"C).":[423],"CONCLUSIONS:":[424],"We":[425],"demonstrate":[426],"physiologically":[431],"relevant":[432],"tissues.":[438],"The":[439],"differential":[440],"expression":[441],"vs":[444],"provides":[446],"opportunity":[448],"minimize":[460],"effect":[462],"muscles.":[465],"Source":[466],"Funding:":[468],"AUA/Urology":[469],"Care":[470],"Foundation":[471],"Scholar":[473],"Award":[474],"SUFU":[476],"Chemodenervation":[477],"Grant":[478],"H.T.R01NS080833,":[480],"R01AI132387,":[481],"R01AI139087,":[482],"R21NS106159":[484],"M.D":[486],"\u00a9":[487],"2021":[488,507],"American":[490,509],"Urological":[491,510],"Association":[492,511],"Education":[493,512],"Research,":[495,514],"Inc.FiguresReferencesRelatedDetails":[496],"Volume":[497],"206Issue":[498],"Supplement":[499],"3September":[500],"2021Page:":[501],"e443-e443":[502],"Advertisement":[503,568],"Copyright":[504],"Permissions\u00a9":[506],"Inc.MetricsAuthor":[515],"Information":[516],"Expand":[566],"Loading":[569],"...":[570]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2021-08-16T00:00:00"}