{"id":"https://openalex.org/W3186432004","doi":"https://doi.org/10.1021/acs.analchem.1c02117","title":"Rapid Highly-Efficient Digestion and Peptide Mapping of Adeno-Associated Viruses","display_name":"Rapid Highly-Efficient Digestion and Peptide Mapping of Adeno-Associated Viruses","publication_year":2021,"publication_date":"2021-07-22","ids":{"openalex":"https://openalex.org/W3186432004","doi":"https://doi.org/10.1021/acs.analchem.1c02117","mag":"3186432004","pmid":"https://pubmed.ncbi.nlm.nih.gov/34291903","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8743033"},"language":"en","primary_location":{"id":"doi:10.1021/acs.analchem.1c02117","doi":"https://doi.org/10.1021/acs.analchem.1c02117","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.analchem.1c02117","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"editorial","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8743033/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087006882","display_name":"Estee Naggar Toole","orcid":"https://orcid.org/0000-0002-0785-1073","observed_orcids":["https://orcid.org/0000-0002-0785-1073"]},"institutions":[{"id":"https://openalex.org/I12912129","display_name":"Northeastern University","ror":"https://ror.org/04t5xt781","country_code":"US","type":"education","lineage":["https://openalex.org/I12912129"]},{"id":"https://openalex.org/I4210142480","display_name":"Thermo Fisher Scientific (United States)","ror":"https://ror.org/03x1ewr52","country_code":"US","type":"company","lineage":["https://openalex.org/I4210142480"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Estee Naggar Toole","raw_affiliation_strings":["Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States","Thermo Fisher Scientific, West Palm Beach, Florida 33401, United States"],"raw_orcid":"https://orcid.org/0000-0002-0785-1073","affiliations":[{"raw_affiliation_string":"Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States","institution_ids":["https://openalex.org/I12912129"]},{"raw_affiliation_string":"Thermo Fisher Scientific, West Palm Beach, Florida 33401, United States","institution_ids":["https://openalex.org/I4210142480"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016499640","display_name":"Craig P. Dufresne","orcid":"https://orcid.org/0000-0003-0785-7798","observed_orcids":["https://orcid.org/0000-0003-0785-7798"]},"institutions":[{"id":"https://openalex.org/I4210142480","display_name":"Thermo Fisher Scientific (United States)","ror":"https://ror.org/03x1ewr52","country_code":"US","type":"company","lineage":["https://openalex.org/I4210142480"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Craig Dufresne","raw_affiliation_strings":["Thermo Fisher Scientific, West Palm Beach, Florida 33401, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Thermo Fisher Scientific, West Palm Beach, Florida 33401, United States","institution_ids":["https://openalex.org/I4210142480"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040252979","display_name":"Somak Ray","orcid":"https://orcid.org/0000-0002-9817-6033","observed_orcids":["https://orcid.org/0000-0002-9817-6033"]},"institutions":[{"id":"https://openalex.org/I12912129","display_name":"Northeastern University","ror":"https://ror.org/04t5xt781","country_code":"US","type":"education","lineage":["https://openalex.org/I12912129"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Somak Ray","raw_affiliation_strings":["Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States","institution_ids":["https://openalex.org/I12912129"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021078562","display_name":"Alexander Boris Schwann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210100141","display_name":"Thermo Fisher Scientific (Switzerland)","ror":"https://ror.org/012wakq07","country_code":"CH","type":"company","lineage":["https://openalex.org/I4210100141","https://openalex.org/I4210142480"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Alexander Schwann","raw_affiliation_strings":["Thermo Fisher Scientific, Neuhofstrasse 11, 4153 Reinach, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Thermo Fisher Scientific, Neuhofstrasse 11, 4153 Reinach, Switzerland","institution_ids":["https://openalex.org/I4210100141"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036992593","display_name":"Ken Cook","orcid":"https://orcid.org/0000-0001-5176-7736","observed_orcids":["https://orcid.org/0000-0001-5176-7736"]},"institutions":[{"id":"https://openalex.org/I4210126472","display_name":"Thermo Fisher Scientific (United Kingdom)","ror":"https://ror.org/03bndes49","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210126472","https://openalex.org/I4210142480"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Ken Cook","raw_affiliation_strings":["Thermo Fisher Scientific, Stafford House, 1 Boundary Park, Hemel Hempstead HP2 7GE, United Kingdom"],"raw_orcid":"https://orcid.org/0000-0001-5176-7736","affiliations":[{"raw_affiliation_string":"Thermo Fisher Scientific, Stafford House, 1 Boundary Park, Hemel Hempstead HP2 7GE, United Kingdom","institution_ids":["https://openalex.org/I4210126472"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5065359511","display_name":"Alexander R. Ivanov","orcid":"https://orcid.org/0000-0002-4691-8488","observed_orcids":["https://orcid.org/0000-0002-4691-8488"]},"institutions":[{"id":"https://openalex.org/I12912129","display_name":"Northeastern University","ror":"https://ror.org/04t5xt781","country_code":"US","type":"education","lineage":["https://openalex.org/I12912129"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Alexander R. Ivanov","raw_affiliation_strings":["Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States"],"raw_orcid":"https://orcid.org/0000-0002-4691-8488","affiliations":[{"raw_affiliation_string":"Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States","institution_ids":["https://openalex.org/I12912129"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5065359511"],"corresponding_institution_ids":["https://openalex.org/I12912129"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":23,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"93","issue":"30","first_page":"10403","last_page":"10410"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.5669000148773193,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.5669000148773193,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.1615999937057495,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.08900000154972076,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adeno-associated-virus","display_name":"adeno-associated virus","score":0.9869999885559082},{"id":"https://openalex.org/keywords/peptide-mapping","display_name":"peptide mapping","score":0.8050000071525574},{"id":"https://openalex.org/keywords/capsid-protein","display_name":"capsid protein","score":0.6259999871253967},{"id":"https://openalex.org/keywords/vp1","display_name":"VP1","score":0.6320000290870667},{"id":"https://openalex.org/keywords/pepsin-digestion","display_name":"pepsin digestion","score":0.8510000109672546},{"id":"https://openalex.org/keywords/gene-therapy-vectors","display_name":"gene therapy vectors","score":0.6290000081062317},{"id":"https://openalex.org/keywords/protein-sequence-coverage","display_name":"protein sequence coverage","score":0.38199999928474426}],"concepts":[{"id":"https://openalex.org/C202878990","wikidata":"https://www.wikidata.org/wiki/Q830465","display_name":"Capsid","level":3,"score":0.8943302035331726},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8324401378631592},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.7107151746749878},{"id":"https://openalex.org/C2781357212","wikidata":"https://www.wikidata.org/wiki/Q5275608","display_name":"Digestion (alchemy)","level":2,"score":0.5081849098205566},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.48254793882369995},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4255714416503906},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.4249264597892761},{"id":"https://openalex.org/C2781047461","wikidata":"https://www.wikidata.org/wiki/Q679692","display_name":"Biopharmaceutical","level":2,"score":0.4246191084384918},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.41988909244537354},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3723676800727844},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.23054465651512146},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.10513514280319214},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.09156674146652222},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000229","descriptor_name":"Dependovirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002213","descriptor_name":"Capsid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004063","descriptor_name":"Digestion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010449","descriptor_name":"Peptide Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D036022","descriptor_name":"Capsid Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1021/acs.analchem.1c02117","doi":"https://doi.org/10.1021/acs.analchem.1c02117","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.analchem.1c02117","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:34291903","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34291903","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8743033","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8743033/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Anal Chem","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:8743033","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8743033/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Anal Chem","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1148643047","display_name":null,"funder_award_id":"R01CA218500","funder_id":"https://openalex.org/F4320306080","funder_display_name":"Foundation for the National Institutes of Health"},{"id":"https://openalex.org/G1678693847","display_name":null,"funder_award_id":"R01GM120272 (ARI)","funder_id":"https://openalex.org/F4320306080","funder_display_name":"Foundation for the National Institutes of Health"},{"id":"https://openalex.org/G5353294128","display_name":null,"funder_award_id":"R35GM136421 (ARI)","funder_id":"https://openalex.org/F4320306080","funder_display_name":"Foundation for the National Institutes of Health"},{"id":"https://openalex.org/G5816863262","display_name":null,"funder_award_id":"R35GM136421","funder_id":"https://openalex.org/F4320306080","funder_display_name":"Foundation for the National Institutes of Health"},{"id":"https://openalex.org/G6720513513","display_name":"Deep Proteomic Profiling of Rare Cells","funder_award_id":"R01GM120272","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G7158645798","display_name":"Effect of methodological and biological variability on molecular profiling of extracellular vesicles in cancer detection","funder_award_id":"R01CA218500","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G7775587985","display_name":null,"funder_award_id":"R01GM120272","funder_id":"https://openalex.org/F4320306080","funder_display_name":"Foundation for the National Institutes of Health"},{"id":"https://openalex.org/G8174178988","display_name":null,"funder_award_id":"R01CA218500 (ARI)","funder_id":"https://openalex.org/F4320306080","funder_display_name":"Foundation for the National Institutes of Health"},{"id":"https://openalex.org/G9000856280","display_name":"Robust ultra-high sensitivity proteomic technologies for limited samples","funder_award_id":"R35GM136421","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320306080","display_name":"Foundation for the National Institutes of Health","ror":"https://ror.org/00k86s890"},{"id":"https://openalex.org/F4320310151","display_name":"Dana-Farber Cancer Institute","ror":"https://ror.org/02jzgtq86"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W1051909975","https://openalex.org/W1148361150","https://openalex.org/W1967200408","https://openalex.org/W1967931040","https://openalex.org/W1969538192","https://openalex.org/W1983984493","https://openalex.org/W2000298298","https://openalex.org/W2035778653","https://openalex.org/W2046488302","https://openalex.org/W2075922302","https://openalex.org/W2085981159","https://openalex.org/W2087386675","https://openalex.org/W2102998463","https://openalex.org/W2103525510","https://openalex.org/W2106356549","https://openalex.org/W2111051741","https://openalex.org/W2111240083","https://openalex.org/W2157464327","https://openalex.org/W2166737662","https://openalex.org/W2334166582","https://openalex.org/W2625692031","https://openalex.org/W2692664495","https://openalex.org/W2739319013","https://openalex.org/W2787783783","https://openalex.org/W2896754864","https://openalex.org/W3044195134","https://openalex.org/W3128082495","https://openalex.org/W4298904855"],"related_works":["https://openalex.org/W3205366844","https://openalex.org/W3185499745","https://openalex.org/W4200205927","https://openalex.org/W2111526115","https://openalex.org/W7100142744","https://openalex.org/W2625692031","https://openalex.org/W4280498685","https://openalex.org/W4323074874","https://openalex.org/W4390022860","https://openalex.org/W4302011899"],"abstract_inverted_index":{"Adeno-associated":[0],"viruses":[1],"(AAVs)":[2],"comprise":[3],"an":[4,90],"area":[5],"of":[6,67,76,139,158,163,183],"rapidly":[7],"growing":[8],"interest":[9],"due":[10,108],"to":[11,14,39,61,109,119,197,202],"their":[12,68],"ability":[13],"act":[15],"as":[16],"a":[17,32,190],"gene":[18,23],"delivery":[19],"vehicle":[20],"in":[21,35,80,133],"novel":[22],"therapy":[24],"strategies":[25],"and":[26,49,57,72,116,147,156,199],"vaccine":[27],"development.":[28],"Peptide":[29],"mapping":[30,54,162],"is":[31,55,104,117,128],"common":[33],"technique":[34],"the":[36,41,73,81,140,154,164,184,204],"biopharmaceutical":[37],"industry":[38],"confirm":[40],"correct":[42],"sequence,":[43],"product":[44],"purity,":[45],"post-translational":[46],"modifications":[47],"(PTMs),":[48],"stability.":[50],"However,":[51],"conventional":[52],"peptide":[53,92,161],"time-consuming":[56],"has":[58],"proven":[59],"difficult":[60],"reproduce":[62],"with":[63,174,189],"viral":[64,144,166],"capsids":[65],"because":[66],"high":[69],"structural":[70],"stability":[71],"suboptimal":[74],"localization":[75],"trypsin":[77],"cleavage":[78,149],"sites":[79],"AAV":[82],"protein":[83,188],"sequences.":[84],"In":[85],"this":[86],"study,":[87],"we":[88,178],"present":[89],"optimized":[91],"mapping-based":[93],"workflow":[94,103],"that":[95],"provides":[96],"thorough":[97],"characterization":[98],"within":[99],"1":[100],"day.":[101],"This":[102],"also":[105],"highly":[106],"reproducible":[107],"its":[110],"simplicity":[111],"having":[112],"very":[113],"few":[114],"steps":[115],"easy":[118],"perform":[120],"proteolytic":[121],"digestion":[122,159,191],"utilizing":[123],"thermally":[124],"stable":[125],"pepsin,":[126],"which":[127],"active":[129],"at":[130],"70":[131],"\u00b0C":[132],"acidic":[134,137],"conditions.":[135],"The":[136],"conditions":[138],"peptic":[141],"digestions":[142],"drive":[143],"capsid":[145,167,187],"denaturation":[146],"improve":[148],"site":[150],"accessibility.":[151],"We":[152],"characterized":[153],"efficiency":[155],"ease":[157],"through":[160],"AAV2":[165],"protein.":[168],"Using":[169],"nanoflow":[170],"liquid":[171],"chromatography":[172],"coupled":[173],"tandem":[175],"mass":[176],"spectrometry,":[177],"achieved":[179],"100%":[180],"sequence":[181],"coverage":[182],"low-abundance":[185],"VP1":[186],"process":[192],"taking":[193],"only":[194],"10":[195],"min":[196,201],"prepare":[198],"45":[200],"complete":[203],"digestion.":[205]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":8},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2021-08-02T00:00:00"}