{"id":"https://openalex.org/W3176562370","doi":"https://doi.org/10.1096/fasebj.2018.32.1_supplement.686.11","title":"Structural Insights into Phospholipase C\u025b Activity","display_name":"Structural Insights into Phospholipase C\u025b Activity","publication_year":2018,"publication_date":"2018-04-01","ids":{"openalex":"https://openalex.org/W3176562370","doi":"https://doi.org/10.1096/fasebj.2018.32.1_supplement.686.11","mag":"3176562370"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.2018.32.1_supplement.686.11","doi":"https://doi.org/10.1096/fasebj.2018.32.1_supplement.686.11","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.2018.32.1_supplement.686.11","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070571932","display_name":"Elisabeth E. Garland\u2010Kuntz","orcid":"https://orcid.org/0000-0003-0397-8998","observed_orcids":["https://orcid.org/0000-0003-0397-8998"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Elisabeth Erin Garland\u2010Kuntz","raw_affiliation_strings":["Chemistry Purdue University West Lafayette IN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Purdue University West Lafayette IN","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032952821","display_name":"Frank S. Vago","orcid":"https://orcid.org/0000-0002-8071-944X","observed_orcids":["https://orcid.org/0000-0002-8071-944X"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Frank S. Vago","raw_affiliation_strings":["Biological Sciences Purdue University West Lafayette IN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Sciences Purdue University West Lafayette IN","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060445526","display_name":"Michelle M. Van Camp","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michelle M. Van Camp","raw_affiliation_strings":["Chemistry Purdue University West Lafayette IN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Purdue University West Lafayette IN","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091146367","display_name":"Monita Sieng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Monita Sieng","raw_affiliation_strings":["Chemistry Purdue University West Lafayette IN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Purdue University West Lafayette IN","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063820728","display_name":"Arryn T. Blaine","orcid":"https://orcid.org/0000-0002-8678-7531","observed_orcids":["https://orcid.org/0000-0002-8678-7531"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Arryn T. Blaine","raw_affiliation_strings":["Chemistry Purdue University West Lafayette IN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Purdue University West Lafayette IN","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031540622","display_name":"Wen Jiang","orcid":"https://orcid.org/0000-0002-1292-2411","observed_orcids":["https://orcid.org/0000-0002-1292-2411"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wen Jiang","raw_affiliation_strings":["Biological Sciences Purdue University West Lafayette IN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Sciences Purdue University West Lafayette IN","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5044423979","display_name":"Angeline M. Lyon","orcid":"https://orcid.org/0000-0001-7501-0148","observed_orcids":["https://orcid.org/0000-0001-7501-0148"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Angeline M. Lyon","raw_affiliation_strings":["Biological Sciences Purdue University West Lafayette IN","Chemistry Purdue University West Lafayette IN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Sciences Purdue University West Lafayette IN","institution_ids":["https://openalex.org/I219193219"]},{"raw_affiliation_string":"Chemistry Purdue University West Lafayette IN","institution_ids":["https://openalex.org/I219193219"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I219193219"],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.22074538,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"32","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.9740999937057495,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.9740999937057495,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.022199999541044235,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phospholipase-c-epsilon","display_name":"phospholipase C epsilon","score":0.972000002861023},{"id":"https://openalex.org/keywords/phospholipase-c-beta","display_name":"phospholipase C beta","score":0.8619999885559082},{"id":"https://openalex.org/keywords/ph-domain","display_name":"pleckstrin homology domain","score":0.8410000205039978},{"id":"https://openalex.org/keywords/conformational-heterogeneity","display_name":"conformational heterogeneity","score":0.8209999799728394},{"id":"https://openalex.org/keywords/solution-structure","display_name":"solution structure","score":0.8730000257492065},{"id":"https://openalex.org/keywords/enzyme-activity","display_name":"enzyme activity","score":0.49900001287460327},{"id":"https://openalex.org/keywords/membrane-binding","display_name":"membrane binding","score":0.5690000057220459},{"id":"https://openalex.org/keywords/inositol-1-4-5-trisphosphate","display_name":"inositol 1,4,5-trisphosphate","score":0.8009999990463257},{"id":"https://openalex.org/keywords/diacylglycerol","display_name":"diacylglycerol","score":0.996999979019165},{"id":"https://openalex.org/keywords/protein-kinase-c","display_name":"protein kinase C","score":0.6169999837875366},{"id":"https://openalex.org/keywords/cardiac-hypertrophy","display_name":"cardiac hypertrophy","score":0.7419999837875366},{"id":"https://openalex.org/keywords/oncogenic-signaling","display_name":"oncogenic signaling","score":0.7099999785423279},{"id":"https://openalex.org/keywords/g-protein-coupled-receptors","display_name":"G protein-coupled receptors","score":0.8510000109672546},{"id":"https://openalex.org/keywords/receptor-tyrosine-kinases","display_name":"receptor tyrosine kinase","score":0.8889999985694885},{"id":"https://openalex.org/keywords/enzyme-regulation","display_name":"enzyme regulation","score":0.7710000276565552}],"concepts":[{"id":"https://openalex.org/C2778597717","wikidata":"https://www.wikidata.org/wiki/Q420248","display_name":"Phospholipase C","level":3,"score":0.7616787552833557},{"id":"https://openalex.org/C10522644","wikidata":"https://www.wikidata.org/wiki/Q24775216","display_name":"HAMP domain","level":4,"score":0.5756194591522217},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5678244829177856},{"id":"https://openalex.org/C163235415","wikidata":"https://www.wikidata.org/wiki/Q899814","display_name":"Second messenger system","level":3,"score":0.5631245970726013},{"id":"https://openalex.org/C66135807","wikidata":"https://www.wikidata.org/wiki/Q24726231","display_name":"Gq alpha subunit","level":4,"score":0.5194846391677856},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.500389575958252},{"id":"https://openalex.org/C2777427919","wikidata":"https://www.wikidata.org/wiki/Q407997","display_name":"Inositol","level":3,"score":0.47160014510154724},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.4641052484512329},{"id":"https://openalex.org/C2780610907","wikidata":"https://www.wikidata.org/wiki/Q2273248","display_name":"Phosphatidylinositol","level":3,"score":0.46312791109085083},{"id":"https://openalex.org/C36020004","wikidata":"https://www.wikidata.org/wiki/Q106951674","display_name":"Diacylglycerol kinase","level":4,"score":0.45568370819091797},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44918709993362427},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.44636157155036926},{"id":"https://openalex.org/C78297661","wikidata":"https://www.wikidata.org/wiki/Q10760862","display_name":"Phosphoinositide phospholipase C","level":4,"score":0.42407655715942383},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.4238690435886383},{"id":"https://openalex.org/C12927208","wikidata":"https://www.wikidata.org/wiki/Q210430","display_name":"Phospholipase","level":3,"score":0.4136071503162384},{"id":"https://openalex.org/C49658373","wikidata":"https://www.wikidata.org/wiki/Q24776397","display_name":"Pleckstrin homology domain","level":3,"score":0.4129073917865753},{"id":"https://openalex.org/C135285700","wikidata":"https://www.wikidata.org/wiki/Q38173","display_name":"G protein-coupled receptor","level":3,"score":0.40837928652763367},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.39374715089797974},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.38050535321235657},{"id":"https://openalex.org/C195794163","wikidata":"https://www.wikidata.org/wiki/Q420877","display_name":"Protein kinase C","level":3,"score":0.332541286945343},{"id":"https://openalex.org/C144292202","wikidata":"https://www.wikidata.org/wiki/Q898273","display_name":"Protein domain","level":3,"score":0.30881381034851074},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12461158633232117}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.2018.32.1_supplement.686.11","doi":"https://doi.org/10.1096/fasebj.2018.32.1_supplement.686.11","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.2018.32.1_supplement.686.11","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3171052695","https://openalex.org/W3177434171","https://openalex.org/W4389018528","https://openalex.org/W4225415120","https://openalex.org/W3172303075","https://openalex.org/W4207077248","https://openalex.org/W2893773596","https://openalex.org/W3177299553","https://openalex.org/W6907884422","https://openalex.org/W4414861278"],"abstract_inverted_index":{"Phospholipase":[0],"C":[1,27],"(PLC)":[2],"enzymes":[3,175,198],"hydrolyze":[4],"phosphatidylinositol":[5],"lipids":[6],"to":[7,32,40,138,150,182,226],"produce":[8],"second":[9],"messengers,":[10],"including":[11],"inositol\u20101,4,5\u2010triphosphate":[12],"(IP":[13],"3":[14],")":[15],"and":[16,23,35,42,51,72,86,121,179,199,205],"diacylgycerol":[17],"(DAG),":[18],"which":[19],"increase":[20],"intracellular":[21],"calcium":[22],"activate":[24],"protein":[25],"kinase":[26],"(PKC),":[28],"respectively.":[29],"PLC\u025b":[30,55,95,135],"contributes":[31],"cardiac":[33],"hypertrophy":[34],"contractility,":[36],"as":[37,39],"well":[38],"oncogenic":[41],"inflammatory":[43],"signaling":[44,197],"pathways":[45],"downstream":[46],"of":[47,67,119,133,140,169,195],"receptor":[48],"tyrosine":[49],"kinases":[50],"G":[52],"protein\u2010coupled":[53],"receptors.":[54],"shares":[56],"a":[57],"conserved":[58],"core":[59],"with":[60,244],"other":[61],"PLC":[62],"enzymes,":[63],"but":[64,104],"the":[65,94,112,124,134,141,154,166,170,192,232],"roles":[66,201],"individual":[68],"domains":[69],"in":[70,130,148,161,173,202,248],"activity":[71],"membrane":[73],"binding":[74],"have":[75,91],"not":[76],"been":[77],"established.":[78],"Using":[79],"biochemical":[80],"assays,":[81],"small\u2010angle":[82],"X\u2010ray":[83],"scattering":[84],"(SAXS),":[85],"electron":[87],"microscopy":[88],"(EM),":[89],"we":[90,110],"found":[92],"that":[93,123,139],"PH":[96,125,156,171],"domain":[97,117,126,157,172],"is":[98,105,127,158,176,230,238],"necessary":[99],"for":[100,107,220],"basal":[101],"lipase":[102],"activity,":[103],"dispensable":[106],"stability.":[108],"Importantly,":[109],"provide":[111,187],"first":[113],"structural":[114],"insights":[115,190],"into":[116,191],"organization":[118],"PLC\u025b,":[120],"reveal":[122,146],"conformationally":[128],"heterogeneous":[129],"solution.":[131,162],"Comparisons":[132],"solution":[136,193],"structure":[137],"closely":[142],"related":[143],"PLC\u03b2":[144,155],"enzyme":[145],"that,":[147],"contrast":[149],"its":[151],"crystal":[152],"structure,":[153],"also":[159,164],"mobile":[160],"We":[163],"show":[165],"dynamic":[167],"nature":[168],"these":[174,196],"functionally":[177],"important,":[178],"may":[180],"contribute":[181],"their":[183,200],"regulation.":[184],"These":[185],"findings":[186],"critical":[188],"new":[189],"structures":[194],"cardiovascular":[203],"disease":[204],"cancer.":[206],"Support":[207],"or":[208],"Funding":[209],"Information":[210],"American":[211],"Heart":[212],"Association":[213],"Scientist":[214],"Development":[215],"Grant":[216],"16SDG29920017;":[217],"Purdue":[218],"Center":[219],"Cancer":[221],"Research;":[222],"Showalter":[223],"Foundation;":[224],"all":[225],"A.M.L":[227],"This":[228],"abstract":[229,246],"from":[231],"Experimental":[233],"Biology":[234],"2018":[235],"Meeting.":[236],"There":[237],"no":[239],"full":[240],"text":[241],"article":[242],"associated":[243],"this":[245],"published":[247],"The":[249],"FASEB":[250],"Journal":[251],".":[252]},"counts_by_year":[],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2021-07-05T00:00:00"}