{"id":"https://openalex.org/W3176166370","doi":"https://doi.org/10.3389/fpls.2021.691754","title":"Overcoming Bottlenecks for Metabolic Engineering of Sesquiterpene Production in Tomato Fruits","display_name":"Overcoming Bottlenecks for Metabolic Engineering of Sesquiterpene Production in Tomato Fruits","publication_year":2021,"publication_date":"2021-06-17","ids":{"openalex":"https://openalex.org/W3176166370","doi":"https://doi.org/10.3389/fpls.2021.691754","mag":"3176166370","pmid":"https://pubmed.ncbi.nlm.nih.gov/34220915","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8248349"},"language":"en","primary_location":{"id":"doi:10.3389/fpls.2021.691754","doi":"https://doi.org/10.3389/fpls.2021.691754","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2021.691754","pdf_url":"https://www.frontiersin.org/articles/10.3389/fpls.2021.691754/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/articles/10.3389/fpls.2021.691754/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005438302","display_name":"Michael Gutensohn","orcid":"https://orcid.org/0000-0002-4281-6687","observed_orcids":["https://orcid.org/0000-0002-4281-6687"]},"institutions":[{"id":"https://openalex.org/I12097938","display_name":"West Virginia University","ror":"https://ror.org/011vxgd24","country_code":"US","type":"education","lineage":["https://openalex.org/I12097938"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Michael Gutensohn","raw_affiliation_strings":["Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV, United States","institution_ids":["https://openalex.org/I12097938"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055468288","display_name":"Laura K. Henry","orcid":"https://orcid.org/0000-0003-0037-2114","observed_orcids":["https://orcid.org/0000-0003-0037-2114"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Laura K. Henry","raw_affiliation_strings":["Department of Biochemistry, Purdue University, West Lafayette, IN, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Purdue University, West Lafayette, IN, United States","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080645503","display_name":"Scott A. Gentry","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Scott A. Gentry","raw_affiliation_strings":["Department of Biochemistry, Purdue University, West Lafayette, IN, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Purdue University, West Lafayette, IN, United States","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067437653","display_name":"Joseph H. Lynch","orcid":"https://orcid.org/0000-0003-0685-1562","observed_orcids":["https://orcid.org/0000-0003-0685-1562"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joseph H. Lynch","raw_affiliation_strings":["Department of Biochemistry, Purdue University, West Lafayette, IN, United States","Purdue Center for Plant Biology, Purdue University, West Lafayette, IN, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Purdue University, West Lafayette, IN, United States","institution_ids":["https://openalex.org/I219193219"]},{"raw_affiliation_string":"Purdue Center for Plant Biology, Purdue University, West Lafayette, IN, United States","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101781385","display_name":"Thuong Thi Hong Nguyen","orcid":"https://orcid.org/0000-0003-4054-1832","observed_orcids":["https://orcid.org/0000-0003-4054-1832"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thuong T. H. Nguyen","raw_affiliation_strings":["Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005511937","display_name":"Eran Pichersky","orcid":"https://orcid.org/0000-0002-4343-1535","observed_orcids":["https://orcid.org/0000-0002-4343-1535"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eran Pichersky","raw_affiliation_strings":["Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5033878915","display_name":"Natalia Dudareva","orcid":"https://orcid.org/0000-0003-0777-7763","observed_orcids":["https://orcid.org/0000-0003-0777-7763"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Natalia Dudareva","raw_affiliation_strings":["Department of Biochemistry, Purdue University, West Lafayette, IN, United States","Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, United States","Purdue Center for Plant Biology, Purdue University, West Lafayette, IN, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Purdue University, West Lafayette, IN, United States","institution_ids":["https://openalex.org/I219193219"]},{"raw_affiliation_string":"Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, United States","institution_ids":["https://openalex.org/I219193219"]},{"raw_affiliation_string":"Purdue Center for Plant Biology, Purdue University, West Lafayette, IN, United States","institution_ids":["https://openalex.org/I219193219"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5005438302"],"corresponding_institution_ids":["https://openalex.org/I12097938"],"apc_list":{"value":2950,"currency":"USD","value_usd":2950},"apc_paid":{"value":2950,"currency":"USD","value_usd":2950},"fwci":0.8389,"has_fulltext":true,"cited_by_count":22,"citation_normalized_percentile":{"value":0.67381279,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"12","issue":null,"first_page":"691754","last_page":"691754"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tomato-fruit","display_name":"tomato fruit","score":0.574999988079071},{"id":"https://openalex.org/keywords/terpene-synthase","display_name":"terpene synthase","score":0.7049999833106995},{"id":"https://openalex.org/keywords/nerolidol","display_name":"nerolidol","score":0.8949999809265137},{"id":"https://openalex.org/keywords/linalool","display_name":"linalool","score":0.9990000128746033},{"id":"https://openalex.org/keywords/farnesyl-pyrophosphate","display_name":"farnesyl pyrophosphate","score":0.7239999771118164},{"id":"https://openalex.org/keywords/geranyl-diphosphate","display_name":"geranyl diphosphate","score":0.9660000205039978},{"id":"https://openalex.org/keywords/3-hydroxy-3-methylglutaryl-coenzyme-a-reductase","display_name":"HMGR","score":0.5360000133514404},{"id":"https://openalex.org/keywords/dxs","display_name":"1-deoxy-D-xylulose 5-phosphate synthase","score":0.8690000176429749},{"id":"https://openalex.org/keywords/fruit-ripening","display_name":"fruit ripening","score":0.48399999737739563},{"id":"https://openalex.org/keywords/antirrhinum-majus","display_name":"Antirrhinum majus","score":0.781000018119812}],"concepts":[{"id":"https://openalex.org/C2778905741","wikidata":"https://www.wikidata.org/wiki/Q72494054","display_name":"Nerolidol","level":4,"score":0.9638311862945557},{"id":"https://openalex.org/C2779729912","wikidata":"https://www.wikidata.org/wiki/Q410932","display_name":"Linalool","level":3,"score":0.8726496696472168},{"id":"https://openalex.org/C44937612","wikidata":"https://www.wikidata.org/wiki/Q212364","display_name":"Terpene","level":2,"score":0.73345947265625},{"id":"https://openalex.org/C206103511","wikidata":"https://www.wikidata.org/wiki/Q426694","display_name":"Terpenoid","level":2,"score":0.7314266562461853},{"id":"https://openalex.org/C2776211264","wikidata":"https://www.wikidata.org/wiki/Q80218","display_name":"Sesquiterpene","level":2,"score":0.7190810441970825},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.7168866395950317},{"id":"https://openalex.org/C2777671706","wikidata":"https://www.wikidata.org/wiki/Q32528","display_name":"Monoterpene","level":2,"score":0.617196798324585},{"id":"https://openalex.org/C2778240251","wikidata":"https://www.wikidata.org/wiki/Q410836","display_name":"Geraniol","level":3,"score":0.49209874868392944},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.47008016705513},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.46150830388069153},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.45555150508880615},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3701106905937195},{"id":"https://openalex.org/C2777278459","wikidata":"https://www.wikidata.org/wiki/Q170885","display_name":"Essential oil","level":2,"score":0.3160335421562195},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.11922770738601685}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3389/fpls.2021.691754","doi":"https://doi.org/10.3389/fpls.2021.691754","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2021.691754","pdf_url":"https://www.frontiersin.org/articles/10.3389/fpls.2021.691754/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"},{"id":"pmid:34220915","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34220915","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in plant science","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8248349","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8248349/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Plant Sci","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:f40397945b864b7dbfb5ea61ecd34c18","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/f40397945b864b7dbfb5ea61ecd34c18","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Plant Science, Vol 12 (2021)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fpls.2021.691754","doi":"https://doi.org/10.3389/fpls.2021.691754","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2021.691754","pdf_url":"https://www.frontiersin.org/articles/10.3389/fpls.2021.691754/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.6875,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4119715150","display_name":null,"funder_award_id":"177845","funder_id":"https://openalex.org/F4320306114","funder_display_name":"U.S. Department of Agriculture"},{"id":"https://openalex.org/G6374980157","display_name":null,"funder_award_id":"177845","funder_id":"https://openalex.org/F4320332299","funder_display_name":"National Institute of Food and Agriculture"},{"id":"https://openalex.org/G7682603709","display_name":null,"funder_award_id":"WVA00730","funder_id":"https://openalex.org/F4320311274","funder_display_name":"West Virginia University"},{"id":"https://openalex.org/G8177196356","display_name":null,"funder_award_id":"WVA00730","funder_id":"https://openalex.org/F4320306114","funder_display_name":"U.S. Department of Agriculture"}],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"},{"id":"https://openalex.org/F4320309036","display_name":"Purdue University","ror":"https://ror.org/02dqehb95"},{"id":"https://openalex.org/F4320311274","display_name":"West Virginia University","ror":"https://ror.org/011vxgd24"},{"id":"https://openalex.org/F4320332299","display_name":"National Institute of Food and Agriculture","ror":"https://ror.org/05qx3fv49"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3176166370.pdf","grobid_xml":"https://content.openalex.org/works/W3176166370.grobid-xml"},"referenced_works_count":66,"referenced_works":["https://openalex.org/W276882634","https://openalex.org/W1514654196","https://openalex.org/W1527930169","https://openalex.org/W1603371265","https://openalex.org/W1669895398","https://openalex.org/W1937587271","https://openalex.org/W1965628579","https://openalex.org/W1967039923","https://openalex.org/W1976961269","https://openalex.org/W1990589501","https://openalex.org/W1991163179","https://openalex.org/W1992103687","https://openalex.org/W1994470176","https://openalex.org/W1997191182","https://openalex.org/W1998745287","https://openalex.org/W2003217432","https://openalex.org/W2007484902","https://openalex.org/W2024426942","https://openalex.org/W2026688176","https://openalex.org/W2032796248","https://openalex.org/W2034815044","https://openalex.org/W2040781918","https://openalex.org/W2046331410","https://openalex.org/W2048710714","https://openalex.org/W2052212755","https://openalex.org/W2053833571","https://openalex.org/W2060003782","https://openalex.org/W2062382198","https://openalex.org/W2062474632","https://openalex.org/W2066194007","https://openalex.org/W2085685413","https://openalex.org/W2086967972","https://openalex.org/W2094639130","https://openalex.org/W2097691925","https://openalex.org/W2098437048","https://openalex.org/W2101127344","https://openalex.org/W2101940514","https://openalex.org/W2108488573","https://openalex.org/W2110763841","https://openalex.org/W2121021883","https://openalex.org/W2122587586","https://openalex.org/W2127637303","https://openalex.org/W2130865348","https://openalex.org/W2131486174","https://openalex.org/W2132135175","https://openalex.org/W2134249696","https://openalex.org/W2134982440","https://openalex.org/W2138403893","https://openalex.org/W2138443296","https://openalex.org/W2141093159","https://openalex.org/W2148113442","https://openalex.org/W2151234135","https://openalex.org/W2152447906","https://openalex.org/W2159181928","https://openalex.org/W2161458733","https://openalex.org/W2163873574","https://openalex.org/W2488682942","https://openalex.org/W2509940547","https://openalex.org/W2598844457","https://openalex.org/W2602808539","https://openalex.org/W2732583686","https://openalex.org/W2885171375","https://openalex.org/W2945728116","https://openalex.org/W3000597397","https://openalex.org/W3092367360","https://openalex.org/W4244772439"],"related_works":["https://openalex.org/W4323349345","https://openalex.org/W2488682942","https://openalex.org/W2006131414","https://openalex.org/W3205942187","https://openalex.org/W2487504942","https://openalex.org/W2148113442","https://openalex.org/W2141093159","https://openalex.org/W2161458733","https://openalex.org/W7161235672","https://openalex.org/W2554755349"],"abstract_inverted_index":{"Terpenoids":[0],"are":[1,18,115,177],"a":[2,180,248,312],"large":[3],"and":[4,15,34,60,111,120,138,278,294,304],"diverse":[5],"class":[6],"of":[7,23,31,38,65,72,82,86,150,153,167,226,238,252],"plant":[8],"metabolites":[9],"that":[10,114,237,246],"also":[11],"includes":[12],"volatile":[13],"mono-":[14],"sesquiterpenes":[16],"which":[17,176],"involved":[19],"in":[20,51,75,80,94,117,144,156,173,210,222,228,240,259,266,311],"biotic":[21],"interactions":[22],"plants.":[24],"Due":[25],"to":[26,45,201,215,219,233],"the":[27,35,118,151,168,194,204,260,272,286],"limited":[28],"natural":[29],"availability":[30,152],"these":[32,124,154],"terpenes":[33],"tight":[36],"regulation":[37],"their":[39,49,142],"biosynthesis,":[40],"there":[41],"is":[42,254],"strong":[43],"interest":[44],"introduce":[46],"or":[47],"enhance":[48],"production":[50,81],"crop":[52],"plants":[53,77],"by":[54,179],"metabolic":[55,298],"engineering":[56,64,93],"for":[57,91,187,256],"agricultural,":[58],"pharmaceutical":[59],"industrial":[61],"applications.":[62],"While":[63,207,292],"monoterpenes":[66],"has":[67],"been":[68],"quite":[69],"successful,":[70],"expression":[71,143,296,308],"sesquiterpene":[73,92,205,257,267],"synthases":[74,128,162],"engineered":[76],"frequently":[78],"resulted":[79,310],"only":[83,247,309],"minor":[84],"amounts":[85],"sesquiterpenes.":[87],"To":[88,262],"identify":[89],"bottlenecks":[90],"plants,":[95],"we":[96,269],"have":[97,129],"used":[98],"two":[99,125],"nearly":[100],"identical":[101],"terpene":[102,127,161],"synthases,":[103],"snapdragon":[104],"(":[105],"Antirrhinum":[106],"majus":[107],")":[108],"nerolidol/linalool":[109],"synthase-1":[110],"-2":[112],"(AmNES/LIS-1/-2),":[113],"localized":[116],"cytosol":[119],"plastids,":[121],"respectively.":[122],"Since":[123],"bifunctional":[126],"very":[130],"similar":[131],"catalytic":[132],"properties":[133],"with":[134],"geranyl":[135],"diphosphate":[136,140],"(GPP)":[137],"farnesyl":[139],"(FPP),":[141],"target":[145],"tissues":[146],"allows":[147],"indirect":[148],"determination":[149],"substrates":[155],"both":[157],"subcellular":[158],"compartments.":[159],"Both":[160],"were":[163,199],"expressed":[164],"under":[165],"control":[166],"ripening":[169],"specific":[170],"PG":[171],"promoter":[172],"tomato":[174],"fruits,":[175,224],"characterized":[178],"highly":[181],"active":[182],"terpenoid":[183],"metabolism":[184],"providing":[185],"precursors":[186],"carotenoid":[188],"biosynthesis.":[189],"As":[190],"AmNES/LIS-2":[191,223,241],"fruits":[192,198,212,230],"produced":[193],"monoterpene":[195],"linalool,":[196],"AmNES/LIS-1":[197,211,229],"found":[200],"exclusively":[202],"produce":[203],"nerolidol.":[206],"nerolidol":[208,301],"emission":[209,221],"was":[213,231],"60-":[214],"584-fold":[216],"lower":[217,235],"compared":[218],"linalool":[220],"accumulation":[225],"nerolidol-glucosides":[227],"4-":[232],"14-fold":[234],"than":[236],"linalool-glucosides":[239],"fruits.":[242],"These":[243],"results":[244],"suggest":[245],"relatively":[249],"small":[250],"pool":[251],"FPP":[253],"available":[255],"formation":[258,302],"cytosol.":[261],"potentially":[263],"overcome":[264],"limitations":[265],"production,":[268],"transiently":[270],"co-expressed":[271],"key":[273],"pathway-enzymes":[274],"hydroxymethylglutaryl-CoA":[275],"reductase":[276],"(HMGR)":[277],"1-deoxy-D-xylulose":[279],"5-phosphate":[280],"synthase":[281],"(DXS),":[282],"as":[283,285],"well":[284],"regulator":[287],"isopentenyl":[288],"phosphate":[289],"kinase":[290],"(IPK).":[291],"HMGR":[293],"IPK":[295],"increased":[297],"flux":[299],"toward":[300],"5.7-":[303],"2.9-fold,":[305],"respectively,":[306],"DXS":[307],"2.5-fold":[313],"increase.":[314]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2021-07-05T00:00:00"}