{"id":"https://openalex.org/W3174040938","doi":"https://doi.org/10.1096/fasebj.25.1_supplement.886.1","title":"Unexpected Properties of the Human LINE\u20101 Retrotransposon ORF1p","display_name":"Unexpected Properties of the Human LINE\u20101 Retrotransposon ORF1p","publication_year":2011,"publication_date":"2011-04-01","ids":{"openalex":"https://openalex.org/W3174040938","doi":"https://doi.org/10.1096/fasebj.25.1_supplement.886.1","mag":"3174040938"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.25.1_supplement.886.1","doi":"https://doi.org/10.1096/fasebj.25.1_supplement.886.1","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.25.1_supplement.886.1","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Kathryn E Callahan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kathryn E Callahan","raw_affiliation_strings":["NIDDK, NIH, Bethesda, MD"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIDDK, NIH, Bethesda, MD","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058399280","display_name":"Alison Burgess Hickman","orcid":"https://orcid.org/0000-0001-7666-0249","observed_orcids":["https://orcid.org/0000-0001-7666-0249"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alison B Hickman","raw_affiliation_strings":["NIDDK, NIH, Bethesda, MD"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIDDK, NIH, Bethesda, MD","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109275482","display_name":"Charles E. Jones","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Charles E Jones","raw_affiliation_strings":["NIDDK, NIH, Bethesda, MD"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIDDK, NIH, Bethesda, MD","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074374757","display_name":"Rodolfo Ghirlando","orcid":"https://orcid.org/0000-0003-4880-4959","observed_orcids":["https://orcid.org/0000-0003-4880-4959"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rodolfo Ghirlando","raw_affiliation_strings":["NIDDK, NIH, Bethesda, MD"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIDDK, NIH, Bethesda, MD","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109386937","display_name":"Anthony V. Furano","orcid":"https://orcid.org/0000-0002-4489-6828","observed_orcids":["https://orcid.org/0000-0002-4489-6828"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anthony V Furano","raw_affiliation_strings":["NIDDK, NIH, Bethesda, MD"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIDDK, NIH, Bethesda, MD","institution_ids":["https://openalex.org/I1299303238"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1299303238"],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.52210593,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"25","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10434","display_name":"Chromosomal and Genetic Variations","score":0.9667999744415283,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10434","display_name":"Chromosomal and Genetic Variations","score":0.9667999744415283,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.00430000014603138,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11213","display_name":"Genomic variations and chromosomal abnormalities","score":0.0024999999441206455,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/line-1-retrotransposons","display_name":"LINE-1 retrotransposons","score":0.9879999756813049},{"id":"https://openalex.org/keywords/orf1p","display_name":"ORF1p","score":0.996999979019165},{"id":"https://openalex.org/keywords/nucleic-acid-chaperone","display_name":"nucleic acid chaperone","score":0.8560000061988831},{"id":"https://openalex.org/keywords/dna-duplex-stabilization","display_name":"DNA duplex stabilization","score":0.7099999785423279},{"id":"https://openalex.org/keywords/dna-melting","display_name":"DNA melting","score":0.5590000152587891},{"id":"https://openalex.org/keywords/protein-polymerization","display_name":"protein polymerization","score":0.7179999947547913},{"id":"https://openalex.org/keywords/retrotransposition","display_name":"retrotransposition","score":0.7879999876022339},{"id":"https://openalex.org/keywords/orf2p","display_name":"ORF2p","score":0.5889999866485596},{"id":"https://openalex.org/keywords/baculovirus-expression-system","display_name":"baculovirus expression system","score":0.6499999761581421}],"concepts":[{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.8219000697135925},{"id":"https://openalex.org/C7029365","wikidata":"https://www.wikidata.org/wiki/Q413988","display_name":"Retrotransposon","level":5,"score":0.7704010009765625},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6043913960456848},{"id":"https://openalex.org/C2777271071","wikidata":"https://www.wikidata.org/wiki/Q422529","display_name":"Nuclease","level":3,"score":0.5872761607170105},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5646712779998779},{"id":"https://openalex.org/C126142528","wikidata":"https://www.wikidata.org/wiki/Q3771876","display_name":"Nucleic acid structure","level":4,"score":0.4964495301246643},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4675509035587311},{"id":"https://openalex.org/C4918238","wikidata":"https://www.wikidata.org/wiki/Q121438","display_name":"Transposable element","level":4,"score":0.42618751525878906},{"id":"https://openalex.org/C2775962898","wikidata":"https://www.wikidata.org/wiki/Q5073152","display_name":"Chaperone (clinical)","level":2,"score":0.4256457984447479},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.38103020191192627},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.379592627286911},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37755322456359863},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3284648656845093},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.3026115894317627},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12157642841339111},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.07234957814216614},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.25.1_supplement.886.1","doi":"https://doi.org/10.1096/fasebj.25.1_supplement.886.1","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.25.1_supplement.886.1","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2088307639","https://openalex.org/W7099431743","https://openalex.org/W2121584886","https://openalex.org/W2054236369","https://openalex.org/W2005361700","https://openalex.org/W2114766453","https://openalex.org/W2171669483","https://openalex.org/W4404531747","https://openalex.org/W3004939016","https://openalex.org/W4244205731"],"abstract_inverted_index":{"LINE\u20101":[0],"(L1)":[1],"is":[2,31,43,119,151],"the":[3,15,32,36,85,109,115,125,131,134,139,149,171,179],"dominant":[4],"autonomous":[5],"retrotransposon":[6],"in":[7,40,152,183,187],"mammals":[8],"generating":[9],"~40%":[10],"of":[11,20,165,173],"mammalian":[12,21],"DNA":[13],"over":[14],"last":[16],"100":[17],"million":[18],"years":[19],"evolution.":[22],"L1":[23,33,41,62],"encodes":[24],"two":[25,102],"proteins,":[26],"ORF1p":[27,39,46,77,107,156,182],"and":[28,51,78,93,112,128,137,160,185],"ORF2p.":[29],"ORF2p":[30],"replicase.":[34],"However,":[35,99],"precise":[37],"role":[38],"replication":[42,63],"unknown.":[44],"Mouse":[45],"both":[47],"binds":[48,108,130,136],"nucleic":[49,55,97,166],"acids":[50],"acts":[52],"as":[53,162],"a":[54,163],"acid":[56,167],"chaperone,":[57],"an":[58],"activity":[59,92],"essential":[60],"for":[61,96],"(Martin,":[64],"S.":[65],"2006.":[66],"J.":[67],"Biomed.":[68],"Biotechnol.":[69],"2006:":[70],"ID#45621).":[71],"We":[72,169],"purified":[73],"baculovirus":[74],"expressed":[75],"human":[76],"found":[79],"that":[80],"it":[81,122],"was":[82],"similar":[83],"to":[84,90,178],"mouse":[86],"protein":[87,135,150],"with":[88,114,176],"respect":[89,177],"chaperone":[91],"its":[94],"affinity":[95],"acids.":[98],"we":[100],"uncovered":[101],"unexpected":[103],"properties:":[104],"(1)":[105],"Although":[106],"mismatched":[110,126,140],"dsDNA":[111,141],"ssDNA":[113],"same":[116],"affinity,":[117],"this":[118],"not":[120],"because":[121],"first":[123],"melts":[124],"duplex":[127],"then":[129],"ssDNA.":[132],"Rather,":[133],"stabilizes":[138],"before":[142],"melting":[143],"it,":[144],"which":[145],"only":[146],"occurs":[147],"when":[148],"molar":[153],"excess.":[154],"(2)":[155],"rapidly":[157],"undergoes":[158],"polymerization":[159],"depolymerization":[161],"function":[164],"concentration.":[168],"discuss":[170],"implications":[172],"these":[174],"findings":[175],"biochemical":[180],"properties":[181],"vitro":[184],"retrotransposition":[186],"vivo.":[188]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2021-07-05T00:00:00"}