{"id":"https://openalex.org/W3173383565","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.709.11","title":"Architecture of the PKA RII\u03b2 Holoenzyme","display_name":"Architecture of the PKA RII\u03b2 Holoenzyme","publication_year":2009,"publication_date":"2009-04-01","ids":{"openalex":"https://openalex.org/W3173383565","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.709.11","mag":"3173383565"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.23.1_supplement.709.11","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.709.11","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.23.1_supplement.709.11","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074819049","display_name":"Simon H. J. Brown","orcid":"https://orcid.org/0000-0003-3997-4614","observed_orcids":["https://orcid.org/0000-0003-3997-4614"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Simon Brown","raw_affiliation_strings":["Chemistry and Biochemistry UCSD La Jolla CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry and Biochemistry UCSD La Jolla CA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071641744","display_name":"Jeffrey Copps","orcid":"https://orcid.org/0000-0003-4793-7258","observed_orcids":["https://orcid.org/0000-0003-4793-7258"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeffrey Copps","raw_affiliation_strings":["Chemistry and Biochemistry UCSD La Jolla CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry and Biochemistry UCSD La Jolla CA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007579445","display_name":"Eric V. Smith\u2010Nguyen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eric Smith\u2010Nguyen","raw_affiliation_strings":["Chemistry and Biochemistry UCSD La Jolla CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry and Biochemistry UCSD La Jolla CA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074463638","display_name":"Donald Blumenthal","orcid":"https://orcid.org/0000-0002-8614-1167","observed_orcids":["https://orcid.org/0000-0002-8614-1167"]},"institutions":[{"id":"https://openalex.org/I223532165","display_name":"University of Utah","ror":"https://ror.org/03r0ha626","country_code":"US","type":"education","lineage":["https://openalex.org/I223532165"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Donald K. Blumenthal","raw_affiliation_strings":["Pharmacology and Toxicology University of Utah, n/a UT"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology and Toxicology University of Utah, n/a UT","institution_ids":["https://openalex.org/I223532165"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5045962448","display_name":"Susan Serota Taylor","orcid":"https://orcid.org/0000-0002-7702-6108","observed_orcids":["https://orcid.org/0000-0002-7702-6108"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Susan S. Taylor","raw_affiliation_strings":["Chemistry and Biochemistry UCSD La Jolla CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry and Biochemistry UCSD La Jolla CA","institution_ids":["https://openalex.org/I36258959"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.3502817,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"23","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.4108999967575073,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.4108999967575073,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.4097000062465668,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.1234000027179718,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-kinase-a","display_name":"protein kinase A","score":0.9599999785423279},{"id":"https://openalex.org/keywords/tetrameric-complex","display_name":"tetrameric complex","score":0.5920000076293945},{"id":"https://openalex.org/keywords/camp-binding","display_name":"cAMP binding","score":0.6150000095367432},{"id":"https://openalex.org/keywords/conformational-change","display_name":"conformational change","score":0.8320000171661377},{"id":"https://openalex.org/keywords/deletion-mutants","display_name":"deletion mutants","score":0.6380000114440918},{"id":"https://openalex.org/keywords/small-angle-x-ray-scattering","display_name":"small-angle X-ray scattering","score":0.6819999814033508},{"id":"https://openalex.org/keywords/small-angle-neutron-scattering","display_name":"small-angle neutron scattering","score":0.9929999709129333},{"id":"https://openalex.org/keywords/protein-architecture","display_name":"protein architecture","score":0.4880000054836273},{"id":"https://openalex.org/keywords/disordered-linker","display_name":"disordered linker","score":0.6259999871253967}],"concepts":[{"id":"https://openalex.org/C131888329","wikidata":"https://www.wikidata.org/wiki/Q3027679","display_name":"Small-angle X-ray scattering","level":3,"score":0.8155325055122375},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.810234010219574},{"id":"https://openalex.org/C2778886173","wikidata":"https://www.wikidata.org/wiki/Q53832","display_name":"Tetramer","level":3,"score":0.7746764421463013},{"id":"https://openalex.org/C2779546866","wikidata":"https://www.wikidata.org/wiki/Q531306","display_name":"Dimer","level":2,"score":0.6083820462226868},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5946140289306641},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5722720623016357},{"id":"https://openalex.org/C2777339483","wikidata":"https://www.wikidata.org/wiki/Q647203","display_name":"Conformational change","level":2,"score":0.5347200036048889},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.5346518754959106},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5013647079467773},{"id":"https://openalex.org/C53345823","wikidata":"https://www.wikidata.org/wiki/Q5531554","display_name":"Gene isoform","level":3,"score":0.4938364624977112},{"id":"https://openalex.org/C2780557392","wikidata":"https://www.wikidata.org/wiki/Q523796","display_name":"Linker","level":2,"score":0.48699435591697693},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.2998550832271576},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.252044677734375},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1720336675643921},{"id":"https://openalex.org/C191486275","wikidata":"https://www.wikidata.org/wiki/Q210028","display_name":"Scattering","level":2,"score":0.16533946990966797},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.14866581559181213},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.11369317770004272},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08765202760696411},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.23.1_supplement.709.11","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.709.11","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.23.1_supplement.709.11","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G7349692883","display_name":"Assembly and regulation of dynamic PKA macromolecular signaling systems","funder_award_id":"R01GM034921","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2054968858","https://openalex.org/W2316851197","https://openalex.org/W2001137280","https://openalex.org/W2292375785","https://openalex.org/W2055439983","https://openalex.org/W124556777","https://openalex.org/W2072398950","https://openalex.org/W2766736787","https://openalex.org/W7066616738","https://openalex.org/W1507403816"],"abstract_inverted_index":{"How":[0],"the":[1,16,32,51,62,67,71,94,98,104,110,122,130,142,153,161,174,183,192,198],"dimeric":[2,117],"PKA":[3],"regulatory":[4,41],"(RI":[5],"and":[6,47,73,86,106,139,187],"RII)":[7],"subunits":[8,18],"assemble":[9],"into":[10,165],"an":[11,158],"inactive":[12],"tetrameric":[13],"complex":[14],"with":[15],"catalytic":[17,52],"is":[19,144,195],"very":[20],"isoform":[21],"specific.":[22],"By":[23,54],"solving":[24],"structures":[25],"of":[26,66,97,109],"holoenzyme":[27,163],"complexes,":[28],"we":[29,59,78,113],"now":[30],"appreciate":[31],"global":[33,95],"conformational":[34,100,107,150],"changes":[35],"that":[36,120,194],"take":[37],"place":[38],"in":[39,70,197],"each":[40],"subunit":[42,156],"as":[43],"they":[44],"release":[45],"cAMP":[46,124],"wrap":[48],"themselves":[49],"around":[50],"subunit.":[53],"using":[55],"monomeric":[56],"deletion":[57,185],"mutants":[58],"can":[60],"map":[61,93],"novel":[63],"isoform\u2010specific":[64],"interactions":[65],"inhibition":[68],"sites":[69],"RI":[72],"RII":[74],"subunits.":[75],"In":[76,127],"parallel":[77],"have":[79,114],"used":[80],"small":[81,87],"angle":[82,88],"X\u2010ray":[83],"scattering":[84,90],"(SAXS)":[85],"neutron":[89],"(SANS)":[91],"to":[92,129,191,206],"architectures":[96],"different":[99],"states.":[101],"To":[102],"define":[103],"shape":[105],"dynamics":[108],"RII\u03b2":[111,146,155,162,200],"subunit,":[112,132],"engineered":[115],"a":[116,135,148,166],"mutant,":[118,186],"RII\u03b2(1\u2010280),":[119],"lacks":[121],"second":[123],"binding":[125],"domain.":[126],"contrast":[128],"RII\u03b1":[131],"which":[133],"forms":[134,157],"highly":[136],"asymmetric":[137],"complex,":[138],"RI\u03b1,":[140],"where":[141],"tetramer":[143],"Y\u2010shaped,":[145],"undergoes":[147],"major":[149],"change.":[151],"While":[152],"free":[154,199],"extended":[159],"dimer,":[160],"folds":[164],"compact,":[167],"globular":[168],"conformation.":[169],"Based":[170],"on":[171],"SAXS":[172],"data,":[173],"properties":[175],"required":[176],"for":[177],"this":[178],"change":[179],"are":[180],"included":[181],"within":[182],"RII\u03b2(1\u2010280)":[184],"most":[188],"likely":[189],"relate":[190],"linker":[193],"disordered":[196],"dimer.":[201],"[Supported":[202],"by":[203],"NIH":[204],"GM34921":[205],"SST]":[207]},"counts_by_year":[],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2021-07-05T00:00:00"}