{"id":"https://openalex.org/W3172326375","doi":"https://doi.org/10.3390/antiox10060965","title":"RedEfish: Generation of the Polycistronic mScarlet: GSG-T2A: Ttpa Zebrafish Line","display_name":"RedEfish: Generation of the Polycistronic mScarlet: GSG-T2A: Ttpa Zebrafish Line","publication_year":2021,"publication_date":"2021-06-16","ids":{"openalex":"https://openalex.org/W3172326375","doi":"https://doi.org/10.3390/antiox10060965","mag":"3172326375","pmid":"https://pubmed.ncbi.nlm.nih.gov/34208660","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8235169"},"language":"en","primary_location":{"id":"doi:10.3390/antiox10060965","doi":"https://doi.org/10.3390/antiox10060965","is_oa":true,"landing_page_url":"https://doi.org/10.3390/antiox10060965","pdf_url":"https://www.mdpi.com/2076-3921/10/6/965/pdf?version=1624287779","source":{"id":"https://openalex.org/S2737566431","display_name":"Antioxidants","issn_l":"2076-3921","issn":["2076-3921"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Antioxidants","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/2076-3921/10/6/965/pdf?version=1624287779","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5025270848","display_name":"Brian Head","orcid":"https://orcid.org/0000-0002-7520-2227","observed_orcids":["https://orcid.org/0000-0002-7520-2227"]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brian Head","raw_affiliation_strings":["Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA","Molecular and Cell Biology Program, Oregon State University, Corvallis, OR 97331, USA"],"raw_orcid":"https://orcid.org/0000-0002-7520-2227","affiliations":[{"raw_affiliation_string":"Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA","institution_ids":["https://openalex.org/I131249849"]},{"raw_affiliation_string":"Molecular and Cell Biology Program, Oregon State University, Corvallis, OR 97331, USA","institution_ids":["https://openalex.org/I131249849"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109593116","display_name":"Jane K. La Du","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jane La Du","raw_affiliation_strings":["Department of Environmental Toxicology, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Toxicology, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331, USA","institution_ids":["https://openalex.org/I131249849"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091730097","display_name":"Carrie L. Barton","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Carrie Barton","raw_affiliation_strings":["Department of Environmental Toxicology, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Toxicology, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331, USA","institution_ids":["https://openalex.org/I131249849"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041047024","display_name":"Jie Zhang","orcid":"https://orcid.org/0000-0001-9697-6689","observed_orcids":["https://orcid.org/0000-0001-9697-6689"]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]},{"id":"https://openalex.org/I52158045","display_name":"China Agricultural University","ror":"https://ror.org/04v3ywz14","country_code":"CN","type":"education","lineage":["https://openalex.org/I52158045"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Jie Zhang","raw_affiliation_strings":["College of Science, China Agriculture University, Beijing 100083, China","Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Science, China Agriculture University, Beijing 100083, China","institution_ids":["https://openalex.org/I52158045"]},{"raw_affiliation_string":"Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA","institution_ids":["https://openalex.org/I131249849"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068993905","display_name":"Carmen P. Wong","orcid":"https://orcid.org/0000-0001-8294-2584","observed_orcids":["https://orcid.org/0000-0001-8294-2584"]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Carmen Wong","raw_affiliation_strings":["Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA","institution_ids":["https://openalex.org/I131249849"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085287919","display_name":"Emily Ho","orcid":"https://orcid.org/0000-0002-7687-2820","observed_orcids":["https://orcid.org/0000-0002-7687-2820"]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Emily Ho","raw_affiliation_strings":["Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA","School of Biological and Population Health Sciences, College of Public Health, Oregon State University, Corvallis, OR 97331, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA","institution_ids":["https://openalex.org/I131249849"]},{"raw_affiliation_string":"School of Biological and Population Health Sciences, College of Public Health, Oregon State University, Corvallis, OR 97331, USA","institution_ids":["https://openalex.org/I131249849"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010885167","display_name":"Robert L. Tanguay","orcid":"https://orcid.org/0000-0001-6190-3682","observed_orcids":["https://orcid.org/0000-0001-6190-3682"]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robyn L. Tanguay","raw_affiliation_strings":["Department of Environmental Toxicology, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331, USA"],"raw_orcid":"https://orcid.org/0000-0001-6190-3682","affiliations":[{"raw_affiliation_string":"Department of Environmental Toxicology, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331, USA","institution_ids":["https://openalex.org/I131249849"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5069647536","display_name":"Maret G. Traber","orcid":"https://orcid.org/0000-0002-2892-4024","observed_orcids":["https://orcid.org/0000-0002-2892-4024"]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Maret G. Traber","raw_affiliation_strings":["Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA","School of Biological and Population Health Sciences, College of Public Health, Oregon State University, Corvallis, OR 97331, USA"],"raw_orcid":"https://orcid.org/0000-0002-2892-4024","affiliations":[{"raw_affiliation_string":"Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA","institution_ids":["https://openalex.org/I131249849"]},{"raw_affiliation_string":"School of Biological and Population Health Sciences, College of Public Health, Oregon State University, Corvallis, OR 97331, USA","institution_ids":["https://openalex.org/I131249849"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5069647536"],"corresponding_institution_ids":["https://openalex.org/I131249849"],"apc_list":{"value":2000,"currency":"CHF","value_usd":2160},"apc_paid":{"value":2000,"currency":"CHF","value_usd":2160},"fwci":0.13,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":{"value":0.3531856,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"10","issue":"6","first_page":"965","last_page":"965"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11685","display_name":"Zebrafish Biomedical Research Applications","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11685","display_name":"Zebrafish Biomedical Research Applications","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alpha-tocopherol-transfer-protein","display_name":"alpha-tocopherol transfer protein","score":0.9520000219345093},{"id":"https://openalex.org/keywords/vitamin-e","display_name":"vitamin E","score":0.578000009059906},{"id":"https://openalex.org/keywords/zebrafish-embryonic-development","display_name":"zebrafish embryonic development","score":0.8299999833106995},{"id":"https://openalex.org/keywords/transgenic-zebrafish-lines","display_name":"transgenic zebrafish lines","score":0.859000027179718},{"id":"https://openalex.org/keywords/olfactory-pit","display_name":"olfactory pit","score":0.6449999809265137},{"id":"https://openalex.org/keywords/lateral-line","display_name":"lateral line","score":0.7680000066757202},{"id":"https://openalex.org/keywords/caudal-vertebrae","display_name":"caudal vertebrae","score":0.8410000205039978},{"id":"https://openalex.org/keywords/in-vivo-fluorescence-imaging","display_name":"in vivo fluorescence imaging","score":0.4790000021457672}],"concepts":[{"id":"https://openalex.org/C2776878037","wikidata":"https://www.wikidata.org/wiki/Q169444","display_name":"Zebrafish","level":3,"score":0.9573487639427185},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7496111392974854},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.609161376953125},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.4641486406326294},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4210856258869171},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.38487672805786133},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.37925469875335693},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.3217933475971222}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3390/antiox10060965","doi":"https://doi.org/10.3390/antiox10060965","is_oa":true,"landing_page_url":"https://doi.org/10.3390/antiox10060965","pdf_url":"https://www.mdpi.com/2076-3921/10/6/965/pdf?version=1624287779","source":{"id":"https://openalex.org/S2737566431","display_name":"Antioxidants","issn_l":"2076-3921","issn":["2076-3921"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Antioxidants","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8235169","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8235169","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Antioxidants (Basel)","raw_type":"Text"},{"id":"pmid:34208660","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34208660","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Antioxidants (Basel, Switzerland)","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:727bfea3d7584fd498865ab7403a9423","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/727bfea3d7584fd498865ab7403a9423","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Antioxidants, Vol 10, Iss 6, p 965 (2021)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3390/antiox10060965","doi":"https://doi.org/10.3390/antiox10060965","is_oa":true,"landing_page_url":"https://doi.org/10.3390/antiox10060965","pdf_url":"https://www.mdpi.com/2076-3921/10/6/965/pdf?version=1624287779","source":{"id":"https://openalex.org/S2737566431","display_name":"Antioxidants","issn_l":"2076-3921","issn":["2076-3921"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Antioxidants","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8791306544","display_name":"Pacific Northwest Center for Translational Environmental Health Research","funder_award_id":"P30ES030287","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"}],"funders":[{"id":"https://openalex.org/F4320337361","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3172326375.pdf","grobid_xml":"https://content.openalex.org/works/W3172326375.grobid-xml"},"referenced_works_count":81,"referenced_works":["https://openalex.org/W69152386","https://openalex.org/W1504564486","https://openalex.org/W1528309942","https://openalex.org/W1545772717","https://openalex.org/W1554831422","https://openalex.org/W1665002463","https://openalex.org/W1932232911","https://openalex.org/W1964277117","https://openalex.org/W1975537268","https://openalex.org/W1980699864","https://openalex.org/W1987679415","https://openalex.org/W1989900621","https://openalex.org/W1996904509","https://openalex.org/W2006977912","https://openalex.org/W2007676659","https://openalex.org/W2016600864","https://openalex.org/W2023545713","https://openalex.org/W2023899667","https://openalex.org/W2030607230","https://openalex.org/W2032938045","https://openalex.org/W2039890032","https://openalex.org/W2047757141","https://openalex.org/W2048368606","https://openalex.org/W2052608182","https://openalex.org/W2056975314","https://openalex.org/W2061978941","https://openalex.org/W2066266422","https://openalex.org/W2067813271","https://openalex.org/W2069747031","https://openalex.org/W2081249700","https://openalex.org/W2082162743","https://openalex.org/W2082287281","https://openalex.org/W2082450648","https://openalex.org/W2082631610","https://openalex.org/W2085391061","https://openalex.org/W2094873926","https://openalex.org/W2095316753","https://openalex.org/W2096834231","https://openalex.org/W2113263139","https://openalex.org/W2116200047","https://openalex.org/W2117590145","https://openalex.org/W2122549452","https://openalex.org/W2122844026","https://openalex.org/W2125349610","https://openalex.org/W2138145343","https://openalex.org/W2139193085","https://openalex.org/W2139271958","https://openalex.org/W2139596617","https://openalex.org/W2140837014","https://openalex.org/W2148292178","https://openalex.org/W2151820614","https://openalex.org/W2162060922","https://openalex.org/W2164577521","https://openalex.org/W2169824691","https://openalex.org/W2231801360","https://openalex.org/W2305729456","https://openalex.org/W2327287555","https://openalex.org/W2397926173","https://openalex.org/W2412312909","https://openalex.org/W2432833347","https://openalex.org/W2471116487","https://openalex.org/W2551836638","https://openalex.org/W2571676839","https://openalex.org/W2590192683","https://openalex.org/W2615853094","https://openalex.org/W2655234677","https://openalex.org/W2743783440","https://openalex.org/W2749289774","https://openalex.org/W2768587531","https://openalex.org/W2793562786","https://openalex.org/W2793823516","https://openalex.org/W2885234628","https://openalex.org/W2945320727","https://openalex.org/W2966774173","https://openalex.org/W2972619920","https://openalex.org/W2984196690","https://openalex.org/W3046981728","https://openalex.org/W3087518541","https://openalex.org/W3097871247","https://openalex.org/W3127556672","https://openalex.org/W6601940058"],"related_works":["https://openalex.org/W3174506173","https://openalex.org/W7095309855","https://openalex.org/W2081249700","https://openalex.org/W3087518541","https://openalex.org/W3030761553","https://openalex.org/W913969302","https://openalex.org/W2802829594","https://openalex.org/W7000633651","https://openalex.org/W2026311612","https://openalex.org/W2792921725"],"abstract_inverted_index":{"The":[0,184],"vitamin":[1],"E":[2],"regulatory":[3],"protein,":[4],"the":[5,42,60,71,83,119,130,153,163,167],"alpha-tocopherol":[6],"transfer":[7],"protein":[8,50,155],"(Ttpa),":[9],"is":[10],"necessary":[11],"for":[12,67],"zebrafish":[13,18,26,38],"embryo":[14,19,196],"development.":[15,197],"To":[16],"evaluate":[17],"Ttpa":[20,193],"function,":[21],"we":[22],"generated":[23],"a":[24,188],"fluorescent-tagged":[25],"transgenic":[27],"line":[28,169],"using":[29],"CRISPR-Cas9":[30],"technology.":[31],"One-cell":[32],"stage":[33],"embryos":[34,111],"(from":[35],"Casper":[36],"(colorless)":[37],"adults)":[39],"were":[40,65,90,182],"injected":[41],"mScarlet":[43,72,154],"coding":[44,73],"sequence":[45],"in":[46,93,109,136,158,172],"combination":[47],"with":[48],"cas9":[49],"complexed":[51],"to":[52,76,81,191],"single":[53],"guide":[54],"RNA":[55],"molecule":[56],"targeting":[57],"5'":[58],"of":[59,70],"ttpa":[61],"genomic":[62],"region.":[63],"Embryos":[64],"genotyped":[66],"proper":[68],"insertion":[69],"sequence,":[74],"raised":[75],"adulthood":[77],"and":[78,100,124,146,170],"successively":[79],"in-crossed":[80],"produce":[82],"homozygote":[84],"RedEfish":[85,89,110,131,185],"(mScarlet:":[86],"GSG-T2A:":[87],"Ttpa).":[88],"characterized":[91],"by":[92,134],"vivo":[94],"fluorescence":[95,135],"detection":[96],"at":[97,112],"1,":[98],"7":[99,128],"14":[101],"days":[102],"post-fertilization":[103],"(dpf).":[104],"Fluorescent":[105],"color":[106],"was":[107,116,132,156],"detectable":[108],"1":[113],"dpf;":[114],"it":[115],"distributed":[117,161],"throughout":[118,162],"developing":[120],"brain,":[121],"posterior":[122,143],"tailbud":[123],"yolk":[125,148],"sac.":[126,149],"At":[127],"dpf,":[129],"identifiable":[133],"olfactory":[137,159],"pits,":[138,160],"gill":[139],"arches,":[140],"pectoral":[141],"fins,":[142],"tail":[144],"region":[145],"residual":[147],"Subsequently":[150],"(14":[151],"dpf),":[152],"found":[157],"digestive":[164],"tract,":[165],"along":[166],"lateral":[168],"especially":[171],"caudal":[173],"vertebrae.":[174],"No":[175],"adverse":[176],"morphological":[177],"outcomes":[178],"or":[179],"developmental":[180],"delays":[181],"observed.":[183],"will":[186],"be":[187],"powerful":[189],"model":[190],"study":[192],"function":[194],"during":[195]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2021-06-22T00:00:00"}