{"id":"https://openalex.org/W3159216397","doi":"https://doi.org/10.1038/s41598-021-88391-5","title":"High sensitivity sanger sequencing detection of BRAF mutations in metastatic melanoma FFPE tissue specimens","display_name":"High sensitivity sanger sequencing detection of BRAF mutations in metastatic melanoma FFPE tissue specimens","publication_year":2021,"publication_date":"2021-04-27","ids":{"openalex":"https://openalex.org/W3159216397","doi":"https://doi.org/10.1038/s41598-021-88391-5","mag":"3159216397","pmid":"https://pubmed.ncbi.nlm.nih.gov/33907234","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8079675"},"language":"en","primary_location":{"id":"doi:10.1038/s41598-021-88391-5","doi":"https://doi.org/10.1038/s41598-021-88391-5","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-021-88391-5","pdf_url":"https://www.nature.com/articles/s41598-021-88391-5.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/s41598-021-88391-5.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080230106","display_name":"Lauren Yuxuan Cheng","orcid":"https://orcid.org/0000-0001-7692-5446","observed_orcids":["https://orcid.org/0000-0001-7692-5446"]},"institutions":[{"id":"https://openalex.org/I74775410","display_name":"Rice University","ror":"https://ror.org/008zs3103","country_code":"US","type":"education","lineage":["https://openalex.org/I74775410"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lauren Y. Cheng","raw_affiliation_strings":["Department of Bioengineering, Rice University, 65000 Main St, Houston, TX, 77030, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, Rice University, 65000 Main St, Houston, TX, 77030, USA","institution_ids":["https://openalex.org/I74775410"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008839819","display_name":"Lauren Elaine Haydu","orcid":"https://orcid.org/0000-0002-6360-0199","observed_orcids":["https://orcid.org/0000-0002-6360-0199"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lauren E. Haydu","raw_affiliation_strings":["Department of Surgical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Surgical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100737515","display_name":"Ping Song","orcid":"https://orcid.org/0000-0001-5947-4604","observed_orcids":["https://orcid.org/0000-0001-5947-4604"]},"institutions":[{"id":"https://openalex.org/I74775410","display_name":"Rice University","ror":"https://ror.org/008zs3103","country_code":"US","type":"education","lineage":["https://openalex.org/I74775410"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ping Song","raw_affiliation_strings":["Department of Bioengineering, Rice University, 65000 Main St, Houston, TX, 77030, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, Rice University, 65000 Main St, Houston, TX, 77030, USA","institution_ids":["https://openalex.org/I74775410"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021834083","display_name":"Jianyi Nie","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74775410","display_name":"Rice University","ror":"https://ror.org/008zs3103","country_code":"US","type":"education","lineage":["https://openalex.org/I74775410"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jianyi Nie","raw_affiliation_strings":["Department of Bioengineering, Rice University, 65000 Main St, Houston, TX, 77030, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, Rice University, 65000 Main St, Houston, TX, 77030, USA","institution_ids":["https://openalex.org/I74775410"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067831105","display_name":"Michael T. Tetzlaff","orcid":"https://orcid.org/0000-0001-7899-4138","observed_orcids":["https://orcid.org/0000-0001-7899-4138"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael T. Tetzlaff","raw_affiliation_strings":["Department of Translational Molecular Pathology, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Translational Molecular Pathology, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040776645","display_name":"Lawrence N. Kwong","orcid":"https://orcid.org/0000-0001-8370-1432","observed_orcids":["https://orcid.org/0000-0001-8370-1432"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lawrence N. Kwong","raw_affiliation_strings":["Department of Translational Molecular Pathology, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Translational Molecular Pathology, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081648218","display_name":"Jeffrey E. Gershenwald","orcid":"https://orcid.org/0000-0003-4519-5369","observed_orcids":["https://orcid.org/0000-0003-4519-5369"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeffrey E. Gershenwald","raw_affiliation_strings":["Department of Surgical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Surgical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048327749","display_name":"Michael A. Davies","orcid":"https://orcid.org/0000-0002-0977-0912","observed_orcids":["https://orcid.org/0000-0002-0977-0912"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael A. Davies","raw_affiliation_strings":["Department of Melanoma Medical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Melanoma Medical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016320004","display_name":"David Yu Zhang","orcid":"https://orcid.org/0000-0002-0213-7663","observed_orcids":["https://orcid.org/0000-0002-0213-7663"]},"institutions":[{"id":"https://openalex.org/I74775410","display_name":"Rice University","ror":"https://ror.org/008zs3103","country_code":"US","type":"education","lineage":["https://openalex.org/I74775410"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"David Yu Zhang","raw_affiliation_strings":["Department of Bioengineering, Rice University, 65000 Main St, Houston, TX, 77030, USA. dyz1@rice.edu","Systems, Synthetic, and Physical Biology, Rice University, Houston, TX, USA. dyz1@rice.edu","Systems, Synthetic, and Physical Biology, Rice University, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, Rice University, 65000 Main St, Houston, TX, 77030, USA. dyz1@rice.edu","institution_ids":["https://openalex.org/I74775410"]},{"raw_affiliation_string":"Systems, Synthetic, and Physical Biology, Rice University, Houston, TX, USA. dyz1@rice.edu","institution_ids":["https://openalex.org/I74775410"]},{"raw_affiliation_string":"Systems, Synthetic, and Physical Biology, Rice University, Houston, TX, USA","institution_ids":["https://openalex.org/I74775410"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5016320004"],"corresponding_institution_ids":["https://openalex.org/I74775410"],"apc_list":{"value":1990,"currency":"USD","value_usd":1990},"apc_paid":{"value":1990,"currency":"USD","value_usd":1990},"fwci":1.3589,"has_fulltext":true,"cited_by_count":28,"citation_normalized_percentile":{"value":0.80635733,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":95,"max":98},"biblio":{"volume":"11","issue":"1","first_page":"9043","last_page":"9043"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11533","display_name":"Melanoma and MAPK Pathways","score":0.8125,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11533","display_name":"Melanoma and MAPK Pathways","score":0.8125,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11287","display_name":"Cancer Genomics and Diagnostics","score":0.14509999752044678,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10392","display_name":"Cutaneous Melanoma Detection and Management","score":0.004600000102072954,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/braf-mutation","display_name":"BRAF mutation","score":0.968999981880188},{"id":"https://openalex.org/keywords/metastatic-melanoma","display_name":"metastatic melanoma","score":0.9710000157356262},{"id":"https://openalex.org/keywords/formalin-fixed-paraffin-embedded-tissue","display_name":"formalin-fixed paraffin-embedded tissue","score":0.7139999866485596},{"id":"https://openalex.org/keywords/sanger-sequencing","display_name":"Sanger sequencing","score":0.7710000276565552},{"id":"https://openalex.org/keywords/variant-allele-frequency","display_name":"variant allele frequency","score":0.7570000290870667},{"id":"https://openalex.org/keywords/low-frequency-mutations","display_name":"low-frequency mutations","score":0.621999979019165},{"id":"https://openalex.org/keywords/lymph-node-metastasis","display_name":"lymph node metastasis","score":0.7360000014305115},{"id":"https://openalex.org/keywords/droplet-digital-pcr","display_name":"droplet digital PCR","score":0.7329999804496765},{"id":"https://openalex.org/keywords/immunohistochemistry","display_name":"immunohistochemistry","score":0.8529999852180481},{"id":"https://openalex.org/keywords/subclonal-heterogeneity","display_name":"subclonal heterogeneity","score":0.7039999961853027},{"id":"https://openalex.org/keywords/mutation-detection-sensitivity","display_name":"mutation detection sensitivity","score":0.41100001335144043}],"concepts":[{"id":"https://openalex.org/C76818968","wikidata":"https://www.wikidata.org/wiki/Q181940","display_name":"Sanger sequencing","level":4,"score":0.9563708305358887},{"id":"https://openalex.org/C160798450","wikidata":"https://www.wikidata.org/wiki/Q4230870","display_name":"Concordance","level":2,"score":0.5623537302017212},{"id":"https://openalex.org/C21790070","wikidata":"https://www.wikidata.org/wiki/Q18030215","display_name":"Neuroblastoma RAS viral oncogene homolog","level":5,"score":0.5610753297805786},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5246027708053589},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.5013248920440674},{"id":"https://openalex.org/C164928082","wikidata":"https://www.wikidata.org/wiki/Q6063435","display_name":"Ion semiconductor sequencing","level":4,"score":0.497121125459671},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4558594822883606},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.44039100408554077},{"id":"https://openalex.org/C2776470698","wikidata":"https://www.wikidata.org/wiki/Q21851559","display_name":"V600E","level":4,"score":0.4282796382904053},{"id":"https://openalex.org/C48126324","wikidata":"https://www.wikidata.org/wiki/Q5276136","display_name":"Digital polymerase chain reaction","level":4,"score":0.4278287887573242},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.39585238695144653},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.3718150854110718},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3553188443183899},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3396802246570587},{"id":"https://openalex.org/C2781187634","wikidata":"https://www.wikidata.org/wiki/Q20969980","display_name":"KRAS","level":4,"score":0.06287747621536255}],"mesh":[{"descriptor_ui":"D004252","descriptor_name":"DNA Mutational Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008545","descriptor_name":"Melanoma","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008545","descriptor_name":"Melanoma","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012189","descriptor_name":"Retrospective Studies","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012878","descriptor_name":"Skin Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012878","descriptor_name":"Skin Neoplasms","qualifier_ui":"Q000556","qualifier_name":"secondary","is_major_topic":true},{"descriptor_ui":"D016612","descriptor_name":"Paraffin Embedding","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016707","descriptor_name":"Tissue Fixation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D048493","descriptor_name":"Proto-Oncogene Proteins B-raf","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":6,"locations":[{"id":"doi:10.1038/s41598-021-88391-5","doi":"https://doi.org/10.1038/s41598-021-88391-5","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-021-88391-5","pdf_url":"https://www.nature.com/articles/s41598-021-88391-5.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"doi:10.21203/rs.3.rs-109681/v1","doi":"https://doi.org/10.21203/rs.3.rs-109681/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-109681/v1","pdf_url":"https://www.researchsquare.com/article/rs-109681/v1.pdf?c=1606959927000","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:8079675","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8079675","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sci Rep","raw_type":"Text"},{"id":"pmid:33907234","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33907234","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:a31f95e2d618422bb5e0d6e0b871a339","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/a31f95e2d618422bb5e0d6e0b871a339","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)","raw_type":"article"},{"id":"pmh:oai:repository.rice.edu:1911/110695","doi":null,"is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-021-88391-5.","pdf_url":null,"source":{"id":"https://openalex.org/S4306401268","display_name":"Rice Research Repository (Rice University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I74775410","host_organization_name":"Rice University","host_organization_lineage":["https://openalex.org/I74775410"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Journal article"}],"best_oa_location":{"id":"doi:10.1038/s41598-021-88391-5","doi":"https://doi.org/10.1038/s41598-021-88391-5","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-021-88391-5","pdf_url":"https://www.nature.com/articles/s41598-021-88391-5.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3821986886","display_name":null,"funder_award_id":"21702-5014","funder_id":"https://openalex.org/F4320338478","funder_display_name":"U.S. Army Medical Research Acquisition Activity"},{"id":"https://openalex.org/G3970896122","display_name":null,"funder_award_id":"MD 21702-5014","funder_id":"https://openalex.org/F4320338478","funder_display_name":"U.S. Army Medical Research Acquisition Activity"},{"id":"https://openalex.org/G5172680926","display_name":null,"funder_award_id":"W81XWH1810144","funder_id":"https://openalex.org/F4320338478","funder_display_name":"U.S. Army Medical Research Acquisition Activity"},{"id":"https://openalex.org/G6699535822","display_name":"Highly multiplexed and mutation-sensitive quantitative PCR for cancer diagnostics","funder_award_id":"R01CA203964","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G7523293838","display_name":null,"funder_award_id":"P50 CA093459","funder_id":"https://openalex.org/F4320309684","funder_display_name":"Division of Cancer Prevention, National Cancer Institute"},{"id":"https://openalex.org/G816327597","display_name":null,"funder_award_id":"P50CA093459","funder_id":"https://openalex.org/F4320332521","funder_display_name":"University of Texas MD Anderson Cancer Center"},{"id":"https://openalex.org/G8829139859","display_name":"Prevalence of Rare Passenger Mutations in Biopsy Tissue as Cancer Stratification Markers","funder_award_id":"RP180147","funder_id":"https://openalex.org/F4320308129","funder_display_name":"Cancer Prevention and Research Institute of Texas"},{"id":"https://openalex.org/G979602875","display_name":"The University of Texas M. D. Anderson Cancer Center SPORE in Melanoma","funder_award_id":"P50CA093459","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320308129","display_name":"Cancer Prevention and Research Institute of Texas","ror":"https://ror.org/0003xa228"},{"id":"https://openalex.org/F4320309684","display_name":"Division of Cancer Prevention, National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332521","display_name":"University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320338478","display_name":"U.S. Army Medical Research Acquisition Activity","ror":null},{"id":"https://openalex.org/F4320338488","display_name":"DOD Peer Reviewed Cancer Research Program","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3159216397.pdf","grobid_xml":"https://content.openalex.org/works/W3159216397.grobid-xml"},"referenced_works_count":37,"referenced_works":["https://openalex.org/W1757182694","https://openalex.org/W1950572133","https://openalex.org/W1965370740","https://openalex.org/W1995261749","https://openalex.org/W2035640092","https://openalex.org/W2089135124","https://openalex.org/W2095598060","https://openalex.org/W2096119920","https://openalex.org/W2097995306","https://openalex.org/W2106543129","https://openalex.org/W2121377606","https://openalex.org/W2121545342","https://openalex.org/W2124662474","https://openalex.org/W2128327828","https://openalex.org/W2128542677","https://openalex.org/W2130688310","https://openalex.org/W2132992059","https://openalex.org/W2135925159","https://openalex.org/W2152897456","https://openalex.org/W2160766792","https://openalex.org/W2168711434","https://openalex.org/W2190346722","https://openalex.org/W2220812108","https://openalex.org/W2560367415","https://openalex.org/W2565744835","https://openalex.org/W2605233023","https://openalex.org/W2771036075","https://openalex.org/W2772753306","https://openalex.org/W2793817570","https://openalex.org/W2793856923","https://openalex.org/W2806295244","https://openalex.org/W2917102036","https://openalex.org/W2951231181","https://openalex.org/W2977293565","https://openalex.org/W2999417355","https://openalex.org/W3005246628","https://openalex.org/W6684641524"],"related_works":["https://openalex.org/W1998649993","https://openalex.org/W135216053","https://openalex.org/W3011003431","https://openalex.org/W2514973575","https://openalex.org/W3194719825","https://openalex.org/W1270134304","https://openalex.org/W1900973458","https://openalex.org/W2113716683","https://openalex.org/W2069473958","https://openalex.org/W2075624068"],"abstract_inverted_index":{"Mutations":[0],"in":[1,48],"the":[2,8,41,75,82,143,147],"BRAF":[3,78,92,132,195],"gene":[4],"at":[5,134],"or":[6,37,61,64],"near":[7],"p.":[9],"V600":[10,93],"locus":[11],"are":[12,38],"informative":[13],"for":[14,20,51,129,162,186],"therapy":[15],"selection,":[16],"but":[17,170],"current":[18],"methods":[19,128],"analyzing":[21],"FFPE":[22,100,198],"tissue":[23,103,109],"DNA":[24],"generally":[25],"have":[26,178],"a":[27,173],"limit":[28],"of":[29,31,90,191,208],"detection":[30,188,205],"5%":[32],"variant":[33,43],"allele":[34],"frequency":[35],"(VAF),":[36],"limited":[39],"to":[40,57,96,167],"single":[42],"(V600E).":[44],"These":[45],"can":[46,183],"result":[47],"false":[49,174],"negatives":[50],"samples":[52,130],"with":[53,131],"low":[54,58,193],"VAFs":[55],"due":[56],"tumor":[59],"content":[60],"subclonal":[62],"heterogeneity,":[63],"harbor":[65],"non-V600":[66,213],"mutations.":[67],"Here,":[68],"we":[69,177],"show":[70],"that":[71,180],"Sanger":[72,181,201],"sequencing":[73,182],"using":[74,111],"NuProbe":[76],"VarTrace":[77],"assay,":[79],"based":[80],"on":[81,107],"Blocker":[83],"Displacement":[84],"Amplification":[85],"(BDA)":[86],"technology,":[87],"is":[88],"capable":[89],"detecting":[91],"mutations":[94,133,165,196,214],"down":[95,166],"0.20%":[97],"VAF":[98,164,194],"from":[99,146,197],"lymph":[101],"node":[102],"samples.":[104,199,219],"Comparison":[105],"experiments":[106],"adjacent":[108],"sections":[110],"BDA":[112,150,200],"Sanger,":[113,151],"immunohistochemistry":[114],"(IHC),":[115],"digital":[116],"droplet":[117],"PCR":[118],"(ddPCR),":[119],"and":[120,153,189,206],"NGS":[121,154],"showed":[122],"100%":[123],"concordance":[124],"among":[125],"all":[126],"4":[127],"\u2265":[135],"1%":[136],"VAF,":[137,169],"though":[138],"ddPCR":[139],"did":[140],"not":[141],"distinguish":[142],"V600K":[144],"mutation":[145],"V600E":[148],"mutation.":[149],"ddPCR,":[152],"(with":[155],"orthogonal":[156],"confirmation)":[157],"were":[158],"also":[159,203],"pairwise":[160],"concordant":[161],"lower":[163],"0.26%":[168],"IHC":[171],"produced":[172],"negative.":[175],"Thus,":[176],"shown":[179],"be":[184],"effective":[185],"rapid":[187],"quantitation":[190,207],"multiple":[192],"method":[202],"enabled":[204],"less":[209],"frequent,":[210],"potentially":[211],"actionable":[212],"as":[215],"demonstrated":[216],"by":[217],"synthetic":[218]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":6}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2021-05-10T00:00:00"}