{"id":"https://openalex.org/W3138074373","doi":"https://doi.org/10.1128/aem.02464-20","title":"Separate Upper Pathway Ring Cleavage Dioxygenases Are Required for Growth of Sphingomonas wittichii Strain RW1 on Dibenzofuran and Dibenzo- p -Dioxin","display_name":"Separate Upper Pathway Ring Cleavage Dioxygenases Are Required for Growth of Sphingomonas wittichii Strain RW1 on Dibenzofuran and Dibenzo- p -Dioxin","publication_year":2021,"publication_date":"2021-03-18","ids":{"openalex":"https://openalex.org/W3138074373","doi":"https://doi.org/10.1128/aem.02464-20","mag":"3138074373","pmid":"https://pubmed.ncbi.nlm.nih.gov/33741618","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8208143"},"language":"en","primary_location":{"id":"doi:10.1128/aem.02464-20","doi":"https://doi.org/10.1128/aem.02464-20","is_oa":false,"landing_page_url":"https://doi.org/10.1128/aem.02464-20","pdf_url":null,"source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC8208143","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082939873","display_name":"Thamer Yousif Mutter","orcid":"https://orcid.org/0000-0002-7631-954X","observed_orcids":["https://orcid.org/0000-0002-7631-954X"]},"institutions":[{"id":"https://openalex.org/I102322142","display_name":"Rutgers, The State University of New Jersey","ror":"https://ror.org/05vt9qd57","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142"]},{"id":"https://openalex.org/I27768575","display_name":"University of Anbar","ror":"https://ror.org/055a6gk50","country_code":"IQ","type":"education","lineage":["https://openalex.org/I27768575"]}],"countries":["IQ","US"],"is_corresponding":false,"raw_author_name":"Thamer Y. Mutter","raw_affiliation_strings":["Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, New Jersey, USA","Department of Biology, College of Science, University of Anbar, Ramadi, Iraq"],"raw_orcid":"https://orcid.org/0000-0002-7631-954X","affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, New Jersey, USA","institution_ids":["https://openalex.org/I102322142"]},{"raw_affiliation_string":"Department of Biology, College of Science, University of Anbar, Ramadi, Iraq","institution_ids":["https://openalex.org/I27768575"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5004252995","display_name":"Gerben J. Zylstra","orcid":"https://orcid.org/0000-0003-0419-1353","observed_orcids":["https://orcid.org/0000-0003-0419-1353"]},"institutions":[{"id":"https://openalex.org/I102322142","display_name":"Rutgers, The State University of New Jersey","ror":"https://ror.org/05vt9qd57","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gerben J. Zylstra","raw_affiliation_strings":["Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, New Jersey, USA"],"raw_orcid":"https://orcid.org/0000-0003-0419-1353","affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, New Jersey, USA","institution_ids":["https://openalex.org/I102322142"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5004252995"],"corresponding_institution_ids":["https://openalex.org/I102322142"],"apc_list":null,"apc_paid":null,"fwci":0.3116,"has_fulltext":false,"cited_by_count":9,"citation_normalized_percentile":{"value":0.4608954,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"87","issue":"11","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10324","display_name":"Microbial bioremediation and biosurfactants","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10324","display_name":"Microbial bioremediation and biosurfactants","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10122","display_name":"Toxic Organic Pollutants Impact","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/2307","display_name":"Health, Toxicology and Mutagenesis"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T13736","display_name":"Steroid Chemistry and Biochemistry","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dioxygenase","display_name":"dioxygenase","score":0.781000018119812},{"id":"https://openalex.org/keywords/plasmid-encoded-enzymes","display_name":"plasmid-encoded enzymes","score":0.7149999737739563}],"concepts":[{"id":"https://openalex.org/C2781039906","wikidata":"https://www.wikidata.org/wiki/Q419513","display_name":"Dibenzofuran","level":2,"score":0.6279433965682983},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.5416013598442078},{"id":"https://openalex.org/C2778022156","wikidata":"https://www.wikidata.org/wiki/Q576145","display_name":"Strain (injury)","level":2,"score":0.5129677653312683},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4984736442565918},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.4777061939239502},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.44613319635391235},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40424397587776184},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2896241545677185},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.0762646496295929},{"id":"https://openalex.org/C43369102","wikidata":"https://www.wikidata.org/wiki/Q2307625","display_name":"Fracture (geology)","level":2,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D001572","descriptor_name":"Benzofurans","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001673","descriptor_name":"Biodegradation, Environmental","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004147","descriptor_name":"Dioxins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004785","descriptor_name":"Environmental Pollutants","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D020578","descriptor_name":"Sphingomonas","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D020578","descriptor_name":"Sphingomonas","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D049308","descriptor_name":"Dioxygenases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049308","descriptor_name":"Dioxygenases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1128/aem.02464-20","doi":"https://doi.org/10.1128/aem.02464-20","is_oa":false,"landing_page_url":"https://doi.org/10.1128/aem.02464-20","pdf_url":null,"source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:7468760","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC8208143","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmid:33741618","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33741618","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and environmental microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8208143","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8208143/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:7468760","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC8208143","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G9000906718","display_name":"Environmental, Microbial and Mammalian Biomolecular Responses to AhR Ligands","funder_award_id":"P42ES004911","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"}],"funders":[{"id":"https://openalex.org/F4320326118","display_name":"Higher Committee for Education Development in Iraq","ror":"https://ror.org/00wnaaa58"},{"id":"https://openalex.org/F4320337361","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":45,"referenced_works":["https://openalex.org/W15925722","https://openalex.org/W1606915127","https://openalex.org/W1764228493","https://openalex.org/W1877788388","https://openalex.org/W1901295806","https://openalex.org/W1906753924","https://openalex.org/W1921830981","https://openalex.org/W1963993112","https://openalex.org/W1972010400","https://openalex.org/W1972529480","https://openalex.org/W1973041235","https://openalex.org/W1980110825","https://openalex.org/W1983215674","https://openalex.org/W1998714404","https://openalex.org/W2017818053","https://openalex.org/W2029678365","https://openalex.org/W2040271747","https://openalex.org/W2048901691","https://openalex.org/W2049148151","https://openalex.org/W2061202321","https://openalex.org/W2068464543","https://openalex.org/W2074198920","https://openalex.org/W2079935164","https://openalex.org/W2088901912","https://openalex.org/W2089824415","https://openalex.org/W2096500603","https://openalex.org/W2103205839","https://openalex.org/W2104728938","https://openalex.org/W2105279940","https://openalex.org/W2106496156","https://openalex.org/W2121178227","https://openalex.org/W2122331717","https://openalex.org/W2122416046","https://openalex.org/W2130402515","https://openalex.org/W2135150658","https://openalex.org/W2137213055","https://openalex.org/W2139069433","https://openalex.org/W2144634347","https://openalex.org/W2150889419","https://openalex.org/W2158937196","https://openalex.org/W2161324288","https://openalex.org/W2314225431","https://openalex.org/W2429844382","https://openalex.org/W2991122353","https://openalex.org/W3029377544"],"related_works":["https://openalex.org/W3198500797","https://openalex.org/W6977847992","https://openalex.org/W7018376893","https://openalex.org/W3041271038","https://openalex.org/W2992591797","https://openalex.org/W4206931817","https://openalex.org/W2122416046","https://openalex.org/W1764228493","https://openalex.org/W1906753924","https://openalex.org/W4386588706"],"abstract_inverted_index":{"RW1":[0,63,114],"has":[1,33,45,144],"been":[2,34,42,46],"the":[3,23,31,56,62,86,107,113,141],"subject":[4],"of":[5,12,97,109],"numerous":[6],"investigations,":[7],"because":[8],"it":[9],"is":[10,117],"one":[11],"only":[13,145],"a":[14,98],"few":[15],"strains":[16],"known":[17],"to":[18,53],"grow":[19],"on":[20,112],"DXN":[21,66,93,103,120,137,142],"as":[22],"sole":[24],"carbon":[25],"and":[26,36,58,65,71,92,130],"energy":[27],"source.":[28],"However,":[29],"while":[30],"genome":[32],"sequenced":[35],"several":[37],"DBF":[38,64,91,110],"pathway":[39,80,108,143],"enzymes":[40,59,83,133],"have":[41],"purified,":[43],"there":[44],"very":[47],"little":[48],"research":[49],"using":[50],"physiological":[51],"techniques":[52],"precisely":[54],"identify":[55],"genes":[57],"involved":[60,84,101],"in":[61,85,102],"catabolic":[67,88],"pathways.":[68],"Using":[69],"knockout":[70],"gene":[72],"replacement":[73],"mutagenesis,":[74],"our":[75,124],"work":[76,125],"identifies":[77],"separate":[78],"upper":[79],"ring":[81],"cleavage":[82],"related":[87],"pathways":[89],"for":[90,119,136],"degradation.":[94,121],"The":[95],"identification":[96],"new":[99],"enzyme":[100],"biodegradation":[104],"explains":[105],"why":[106],"degradation":[111],"megaplasmid":[115],"pSWIT02":[116],"inefficient":[118],"In":[122],"addition,":[123],"demonstrates":[126],"that":[127,140],"both":[128],"plasmid-":[129],"chromosomally":[131],"encoded":[132],"are":[134],"necessary":[135],"degradation,":[138],"suggesting":[139],"recently":[146],"evolved.":[147]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2021-03-29T00:00:00"}